Abstract：
:Alkaline phosphatase (AP) activity is expressed in the endodermal cell lineage of ascidian embryos beginning at gastrulation. AP expression is resistant to levels of actinomycin D which completely suppress the appearance of other tissue-specific enzyme and morphological markers including acetylcholinesterase (AchE), a larval muscle enzyme whose expression requires embryonic transcription. The resistance of AP expression to actinomycin D has led to the proposal that AP may be expressed independent of embryonic transcription by the translational activation of maternal AP mRNA. To test this hypothesis, AP expression was examined in fragments of unfertilized and fertilized Styela plicata eggs by histochemical methods. As expected, nucleate fragments from fertilized eggs developed into larvae which exhibited AP activity in their endodermal cells and AchE activity in their muscle cells. In contrast, anucleate fragments from fertilized eggs, cultured until controls reached the larval stage, did not develop AP or AchE activity. The lack of AP activity was unrelated to the absence of cleavage or to the ooplasmic composition of the anucleate fragments. Anucleate fragments from unfertilized eggs were also AP negative, unless they were inseminated, after which they often developed to the larval stage as andromerogons and exhibited AP activity in their endodermal cells. The development of endodermal AP in andromerogons suggests that the factors responsible for AP expression are not localized in or attached to the maternal nucleus. In summary, the results suggest that AP expression requires nuclear events and is not determined exclusively by maternal cytoplasmic factors such as preformed mRNA.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Bates WR,Jeffery WRdoi
10.1016/0012-1606(87)90043-1subject
Has Abstractpub_date
1987-02-01 00:00:00pages
382-9issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(87)90043-1journal_volume
119pub_type
杂志文章abstract：:Ammonium transporter C (AmtC) is one of three transporters in Dictyostelium that have been proposed to regulate entry and exit of ammonia in a cell type dependent manner and to mediate ammonia signaling. Previous work demonstrated that disruption of the amtC gene results in a slugger phenotype in which the cells remai...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2005-11-01 00:00:00
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1990-12-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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doi：10.1016/j.ydbio.2006.06.030
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90260-0
更新日期：1987-04-01 00:00:00
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1996-10-10 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9120
更新日期：1999-02-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1248
更新日期：1994-09-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1002
更新日期：1993-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90370-7
更新日期：1985-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2010-08-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.11.017
更新日期：2016-01-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/0012-1606(85)90461-0
更新日期：1985-06-01 00:00:00
abstract：:Epithelial planar cell polarity (PCP) allows epithelial cells to coordinate their development to that of the tissue in which they reside. The mechanisms that impart PCP as well as effectors that execute the polarizing instructions are being sought in many tissues. We report that the epidermal epithelium of Drosophila ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.04.454
更新日期：2006-09-15 00:00:00
abstract：:For both the intricate morphogenetic layout of the sensory cells in the ear and the elegantly radial arrangement of the sensory neurons in the nose, numerous signaling molecules and genetic determinants are required in concert to generate these specialized neuronal populations that help connect us to our environment. ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
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更新日期：2014-05-01 00:00:00
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journal_title：Developmental biology
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更新日期：2016-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2013-04-15 00:00:00
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journal_title：Developmental biology
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更新日期：2001-05-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2019-11-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00139-8
更新日期：2003-06-15 00:00:00
abstract：:Pioneer axons in insect legs are experimentally accessible model systems for the molecular identification and cellular localization of guidance cues regulating the path of axon growth. A detailed study of the Fe2 pioneer axons in the legs of the cockroach was performed to examine the diversity of guidance mechanisms. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8704
更新日期：1997-10-15 00:00:00
abstract：:Cranial placodes are specialized ectodermal regions in the developing vertebrate head that give rise to both neural and non-neural cell types of the neuroendocrine system and the sense organs of the visual, olfactory and acoustic systems. The cranial placodes develop from a panplacodal region which is specifically mar...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.10.024
更新日期：2011-02-01 00:00:00
abstract：:Transcription factors (TFs) can regulate different sets of genes to determine specific cell types by means of combinatorial codes. We previously identified closely-spaced TF binding motifs located 8.2-8.5 kb 5' to the ATG of the murine Pou4f3 gene, a gene required for late hair cell (HC) differentiation and survival. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.09.002
更新日期：2012-12-01 00:00:00
abstract：:Congenital bony syngnathia, a rare but severe human birth defect, is characterized by bony fusion of the mandible to the maxilla. However, the genetic mechanisms underlying this birth defect are poorly understood, largely due to limitation of available animal models. Here we present evidence that transgenic expression...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.04.013
更新日期：2014-07-15 00:00:00
abstract：:Myoblasts of the L6 rat cell line were grown in Ham's F12 nutrient medium containing 10% fetal calf serum (F12 + FCS). Although the cells were confluent by 6 days in culture, fusion was not observed even if cultures were maintained for 10-14 days. At least 80% of the cells in such confluent unfused cultures were in th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90192-1
更新日期：1984-04-01 00:00:00
abstract：:Sequence analysis of cDNA clones encoding fibronectin (FN) from Xenopus laevis reveals extensive amino acid identities with other vertebrate FNs, including the presence of the Arg-Gly-Asp (RGD) cell attachment site in type III-10 and of a second, cell-binding site (EILDV) in the alternative spliced V region of the pro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90291-n
更新日期：1992-02-01 00:00:00
