Abstract:
:The murine allantois will become the umbilical artery and vein of the chorioallantoic placenta. In previous studies, growth and differentiation of the allantois had been elucidated in whole embryos. In this study, the extent to which explanted allantoises grow and differentiate outside of the conceptus was investigated. The explant model was then used to elucidate cell and growth factor requirements in allantoic development. Early headfold-stage murine allantoises were explanted directly onto tissue culture plastic or suspended in test tubes. Explanted allantoises vascularized with distal-to-proximal polarity, they exhibited many of the same signaling factors used by the vitelline and cardiovascular systems, and they contained at least three cell types whose identity, gene expression profiles, topographical associations, and behavior resembled those of intact allantoises. DiI labeling further revealed that isolated allantoises grew and vascularized in the absence of significant cell mingling, thereby supporting a model of mesodermal differentiation in the allantois that is position- and possibly age-dependent. Manipulation of allantoic explants by varying growth media demonstrated that the allantoic endothelial cell lineage, like that of other embryonic vasculatures, is responsive to VEGF(164). Although VEGF(164) was required for both survival and proliferation of allantoic angioblasts, it was not sufficient to induce appropriate epithelialization of these cells. Rather, other VEGF isoforms and/or the outer sheath of mesothelium, whose maintenance did not appear to be dependent upon endothelium, may also play important roles. On the basis of these findings, we propose murine allantoic explants as a new tool for shedding light not only on allantoic development, but for elucidating universal mechanisms of blood vessel formation, including vascular supporting cells, either in the intact organism or in existing in vitro systems.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Downs KM,Temkin R,Gifford S,McHugh Jdoi
10.1006/dbio.2001.0227subject
Has Abstractpub_date
2001-05-15 00:00:00pages
347-64issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(01)90227-1journal_volume
233pub_type
杂志文章abstract::The mammalian Cul4 genes, Cul4A and Cul4B, encode the scaffold components of the cullin-based E3 ubiquitin ligases. The two Cul4 genes are functionally redundant. Recent study indicated that mice expressing a truncated CUL4A that fails to interact with its functional partner ROC1 exhibit no developmental phenotype. We...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.01.028
更新日期:2011-04-15 00:00:00
abstract::In Xenopus gastrulation, the involuting mesodermal and non-involuting ectodermal cells remain separated from each other, undergoing convergent extension. Here, we show that Eph-ephrin signaling is crucial for the tissue separation and convergence during gastrulation. The loss of EphA4 function results in aberrant gast...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.12.012
更新日期:2011-02-15 00:00:00
abstract::We studied the pathway of 5S ribosomal RNA (rRNA) during oogenesis in Xenopus from its storage in the cytoplasm to incorporation into ribosomes in the nucleus. Ribonucleoprotein particle (RNP) assembly assays reveal striking differences in the behavior of oocyte-type and somatic-type 5S rRNA after microinjection into ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1080
更新日期:1995-04-01 00:00:00
abstract::A monoclonal antibody (MAb No. 25-16), raised against purified cortical secretory vesicles (CVs) from the eggs of Strongylocentrotus purpuratus, has been used to identify a previously uncharacterized CV-derived polypeptide component of the sea urchin fertilization envelope (FE). MAb No. 25-16, an IgG1, bound to a grou...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90210-8
更新日期:1989-03-01 00:00:00
abstract::Mammalian oocytes are arrested in meiotic prophase from around the time of birth until just before ovulation. Following an extended period of growth, they are stimulated to mature to the metaphase II stage by a preovulatory luteinizing hormone (LH) surge that occurs with each reproductive cycle. Small, growing oocytes...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.12.014
更新日期:2018-02-01 00:00:00
abstract::The Hedgehog (Hh) and Notch signal transduction pathways control a variety of developmental processes including cell fate choice, differentiation, proliferation, patterning and boundary formation. Because many components of these pathways are conserved, it was predicted and confirmed that pathway components are largel...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.08.064
更新日期:2006-12-01 00:00:00
abstract::VegT is an essential maternal regulator of germ layer specification in Xenopus. The localization of VegT mRNA to the vegetal cortex of the oocyte during oogenesis ensures its inheritance by vegetal and not animal cells, and directs the differentiation of vegetal cells into endoderm. Similarly localized mRNAs, Vg1 and ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0495
更新日期:2001-12-15 00:00:00
abstract::It has been suggested that the mesonephros has a role in normal limb development. This hypothesis was directly tested by removing the mesonephros adjacent to the presumptive limb region of stage 12-18 chick embryos using microsurgery or laser ablation. The experimental manipulation resulted in reduced limb outgrowth o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90230-e
更新日期:1992-05-01 00:00:00
abstract::Epithelial sheet movement is an essential morphogenetic process during mouse embryonic eyelid closure in which Mitogen-Activated Protein 3 Kinase 1 (MAP3K1) and c-Jun play a critical role. Here we show that MAP3K1 associates with the cytoskeleton, activates Jun N-terminal kinase (JNK) and actin polymerization, and pro...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.09.001
更新日期:2014-11-01 00:00:00
abstract::We have used a subtractive method to clone novel messages enriched in the heart. Here we show that one such message, bves (blood vessel/epicardial substance) is a novel protein that is highly conserved between chicken and mouse. The bves message is detected at high levels in early chick hearts. Using anti-Bves antibod...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9246
更新日期:1999-05-01 00:00:00
abstract::Nucleostemin (NS), a member of a family of nucleolar GTP-binding proteins, is highly expressed in proliferating cells such as stem and cancer cells and is involved in the control of cell cycle progression. Both depletion and overexpression of NS result in stabilization of the tumor suppressor p53 protein in vitro. Alt...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.04.028
更新日期:2011-07-15 00:00:00
abstract::In this paper we present a structural and functional characterization of a new sea urchin embryo transcription factor, SpRunt-1. This factor was isolated by means of its specific interaction with a cis-regulatory target site of the CyIIIa gene. Here we show that this target site, the P7I site, is required for normal e...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0050
更新日期:1996-02-25 00:00:00
abstract::The three fibronectin leucine-rich repeat transmembrane (FLRT) proteins contain 10 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. XFLRT3, a Nodal/TGFbeta target, regulates cell adhesion and modulates FGF signalling during Xenopus gast...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.03.021
更新日期:2008-06-01 00:00:00
abstract::The eukaryotic initiation translation factor eIF6 is a highly conserved, essential protein implicated in translation. eIF6 is regulated in vivo by extracellular signals, such as IGF signaling (for a review see Miluzio et al., 2009). In Xenopus, eif6 over-expression causes a delay in eye development (De Marco et al., 2...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.04.017
更新日期:2017-07-01 00:00:00
abstract::During animal fertilizations, each oocyte or egg must produce a proper intracellular calcium signal for development to proceed normally. As a supplement to recent synopses of fertilization-induced calcium responses in mammals, this paper reviews the spatiotemporal properties of calcium signaling during fertilization a...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1006/dbio.1999.9340
更新日期:1999-07-15 00:00:00
abstract::The germinal vesicle (GV) of starfish oocytes contains a factor which is required to drive the cytoplasmic cycle of the meiotic division. Biochemical investigation of this factor has been difficult due to the small quantities of obtainable GV materials. To overcome this, we have developed a mass-isolation procedure fo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90187-l
更新日期:1992-06-01 00:00:00
abstract::The mammalian Cone-rod homeobox (Crx) gene is a divergent member of the Otx gene family known to be involved in differentiation and survival of retinal photoreceptors and photoentrainment of circadian rhythms. Zebrafish have two genes in the Otx5/crx orthology class, and we previously showed that crx can transactivate...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.01.037
更新日期:2004-05-01 00:00:00
abstract::The cardiac neural crest contains ectomesenchymal and neural anlagen that are necessary for normal heart development. It is not known whether other regions of the neural crest are capable of supporting normal heart development. In the experiments reported herein, quail donor embryos provided cardiac, trunk, or mesence...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90112-7
更新日期:1989-08-01 00:00:00
abstract::Proper patterning and growth of oral structures including teeth, tongue, and palate rely on epithelial-mesenchymal interactions involving coordinated regulation of signal transduction. Understanding molecular mechanisms underpinning oral-facial development will provide novel insights into the etiology of common congen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.05.002
更新日期:2011-08-01 00:00:00
abstract::A new method is presented for preparation of highly purified plasma membranes from fertilized sea urchin eggs. The purified plasma membranes are in vesicle form and are highly enriched in ouabain inhibitable, Na+/K+ ATPase activity. Analysis of membrane proteins by sodium dodecyl sulfate-gel electrophoresis indicates ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90106-9
更新日期:1983-06-01 00:00:00
abstract::N-cadherin is a Ca2+-dependent cell-cell adhesion molecule, which was identified in brain cells of mouse and chicken. In the present study, we have determined the pattern of expression of N-cadherin in chicken embryos at various stages by means of immunohistochemistry. N-cadherin was expressed in cells derived from al...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90119-9
更新日期:1987-03-01 00:00:00
abstract::We report the embryonic phenotype of muscleblind (mbl), a recently described Drosophila gene involved in terminal differentiation of adult ommatidia. mbl is a nuclear protein expressed late in the embryo in pharyngeal, visceral, and somatic muscles, the ventral nerve cord, and the larval photoreceptor system. All thre...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8833
更新日期:1998-03-15 00:00:00
abstract::Myogenesis in the embryo and the adult mammal consists of a highly organized and regulated sequence of cellular processes to form or repair muscle tissue that include cell proliferation, migration, and differentiation. Data from cell culture and in vivo experiments implicate both FGFs and HGF as critical regulators of...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0416
更新日期:2001-11-01 00:00:00
abstract::Only a subset of cleavage stage blastomeres in the Xenopus embryo is competent to contribute cells to the retina; ventral vegetal blastomeres do not form retina even when provided with neuralizing factors or transplanted to the most retinogenic position of the embryo. These results suggest that endogenous maternal fac...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.01.040
更新日期:2007-05-01 00:00:00
abstract::The fate of bacteriophage lambda-DNA was examined after injection into the fertilized eggs of Xenopus laevis. Injection of a large amount of lambda-DNA (ca. 24 ng) into a fertilized Xenopus egg induced the formation around the injected DNA of a giant nucleus-like structure which was surrounded by an apparently normal ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90155-7
更新日期:1986-08-01 00:00:00
abstract::Growth factor signaling regulates tissue-tissue interactions to control organogenesis and tissue homeostasis. Specifically, transforming growth factor beta (TGFβ) signaling plays a crucial role in the development of cranial neural crest (CNC) cell-derived bone, and loss of Tgfbr2 in CNC cells results in craniofacial s...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.02.010
更新日期:2015-04-15 00:00:00
abstract::The correct assembly of junction components, such as E-cadherin and beta-catenin, into the zonula adherens is fundamental for the function of epithelia, both in flies and in vertebrates. In C. elegans, however, the cadherin-catenin system is not essential for general adhesion, raising the question as to the genetic ba...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.0113
更新日期:2001-02-01 00:00:00
abstract::The neural cell adhesion molecule (NCAM) is thought to play a role in the formation of the vertebrate nervous system. In mammals and chicken, it is known that more than 100 different forms of the NCAM protein can be generated by alternative splicing of one primary transcript and it is possible that these different for...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90276-m
更新日期:1992-01-01 00:00:00
abstract::Type II collagen is a major component of cartilage extracellular matrix. Differentiation of mesenchyme into cartilage involves the cessation of type I collagen synthesis and the onset of type II collagen synthesis. Solution hybridization of mRNA isolated from chick limb buds with a cDNA probe to type II collagen mRNA ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90057-7
更新日期:1988-01-01 00:00:00
abstract::Bullous pemphigoid antisera and monoclonal antibodies to type VII collagen were used to localize hemidesmosomes and anchoring fibrils, respectively, in tissues of developing eyes and healing corneal wounds of New Zealand white rabbits. In the 17-day fetal rabbit eye, both antibodies colocalize to the epithelial-stroma...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90136-4
更新日期:1988-04-01 00:00:00