Abstract:
:Type II collagen is a major component of cartilage extracellular matrix. Differentiation of mesenchyme into cartilage involves the cessation of type I collagen synthesis and the onset of type II collagen synthesis. Solution hybridization of mRNA isolated from chick limb buds with a cDNA probe to type II collagen mRNA showed the presence of small amounts of type II collagen message in mesenchymal chick limbs. We have examined the localization of type II collagen mRNA in mesenchymal chick wing buds by in situ hybridization using single stranded RNA probes. Our results show a small but detectable amount of type II collagen RNA distributed uniformly in early limbs until the first precartilage condensations form at stage 22. This is interesting because it is known that mesenchyme isolated from chick wing buds has the capacity to undergo chondrogenesis in culture, even if taken from nonchondrogenic areas of the limb. At stage 23, type II collagen mRNA is found at significantly increased levels in the cells of the precartilage condensation when compared to the other limb cells. As chondrogenesis proceeds, the amount of type II collagen RNA increases even more in cells of the cartilage elements. The signal in the peripheral tissue is indistinguishable from background. These results show that type II collagen message exists at low levels in cells throughout the mesenchymal chick wing bud, until the formation of the condensation results in an elevation of type II mRNA in the prechondrogenic cells found in the core of the limb.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Swalla BJ,Upholt WB,Solursh Mdoi
10.1016/0012-1606(88)90057-7subject
Has Abstractpub_date
1988-01-01 00:00:00pages
51-8issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(88)90057-7journal_volume
125pub_type
杂志文章abstract::In this study, we present evidence that neurogenic cells inhibit the differentiation of cardiogenic cells. When cells of the entire area pellucida at stage 5 were dissociated and reaggregated, the aggregates differentiated into neural tissues and other structures of any germ layer origin, except for heart tissues, des...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1266
更新日期:1995-09-01 00:00:00
abstract::Mf1, which encodes a winged-helix/forkhead transcription factor, is the murine homolog of human FKHL7, mutated in individuals with autosomal dominant inherited dysgenesis of the anterior segment of the eye (Axenfeld-Reiger anomaly). Mouse embryos homozygous for null mutations in Mf1 (Mf1(lacZ) and Mf1(ch)) show severe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9314
更新日期:1999-07-15 00:00:00
abstract::Expression of cardiac troponin I (TnIcardiac) and slow skeletal troponin I (TnIslow) genes was analyzed at the mRNA and protein level in the developing rat heart. TnIslow mRNA was detectable by in situ hybridization in the embryonic cardiac tube as early as the 13-somite stage (Embryonic Day 10). In contrast, TnIcardi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1074
更新日期:1993-03-01 00:00:00
abstract::The formation of the spinal cord during early embryonic development in vertebrate embryos is a continuous process that begins at gastrulation and continues through to the completion of somitogenesis. Despite the conserved usage of patterning mechanisms and gene regulatory networks that act to generate specific spinal ...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2017.01.021
更新日期:2017-12-01 00:00:00
abstract::Despite undergoing normal development and acquiring normal morphology and motility, mutations in spe-38 or trp-3/spe-41 cause identical phenotypes in Caenorhabditis elegans-mutant sperm fail to fertilize oocytes despite direct contact. SPE-38 is a novel, four-pass transmembrane protein and TRP-3/SPE-41 is a Ca(2+)-per...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.02.037
更新日期:2012-05-15 00:00:00
abstract::Neurons of the dorsal hindbrain and spinal cord are central in receiving, processing and relaying sensory perception and participate in the coordination of sensory-motor output. Numerous cellular and molecular mechanisms that underlie neuronal development in both regions of the nervous system are shared. We discuss he...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2016.10.008
更新日期:2017-12-01 00:00:00
abstract::To analyse cell migration and the differentiation potential of migratory stem cells in Hydractinia, we generated animals with an eGFP reporter gene stably expressed and transmitted via the germline. The transgene was placed under the control of two different actin promoters and the promoter of elongation factor-1α. On...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.08.017
更新日期:2010-12-01 00:00:00
abstract::Connective tissue outgrowths of neonatal muscle onto a substratum of bone matrix differentiate into cartilage in response to a bone morphogenetic protein (BMP). The BMP can be separated from bone matrix by extraction with 4 M guanidine hydrochloride (GuHCl) or degraded in situ by endogenous proteolytic enzymes to deac...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90339-9
更新日期:1988-12-01 00:00:00
abstract::The Drosophila brain is tracheated by the cerebral trachea, a branch of the first segmental trachea of the embryo. During larval stages the cerebral trachea splits into several main (primary) branches that grow around the neuropile, forming a perineuropilar tracheal plexus (PNP) at the neuropile surface. Five primary ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.09.022
更新日期:2007-02-01 00:00:00
abstract::How organisms control organ size is not fully understood. We found that Syd/JIP3 is required for proper wing size in Drosophila. JIP3 mutations are associated with organ size defects in mammals. The underlying mechanisms are not well understood. We discovered that Syd/JIP3 inhibition results in a downregulation of the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2020.09.017
更新日期:2021-01-01 00:00:00
abstract::TGF-beta superfamily members signal through a heteromeric receptor complex to regulate craniofacial development. TGF-beta type II receptor appears to bind only TGF-beta, whereas TGF-beta type I receptor (ALK5) also binds to ligands in addition to TGF-beta. Our previous work has shown that conditional inactivation of T...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.03.045
更新日期:2008-08-01 00:00:00
abstract::Cytoskeletal intermediate filaments were studied in muscular dysgenesis (mdg) and tetrodotoxin-treated inactive mouse embryo muscle cultures during myofibrillogenesis. Both muscular dysgenesis and tetrodotoxin-treated muscles are characterized in vitro by a total lack of contractile activity and an abnormal developmen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90159-5
更新日期:1988-09-01 00:00:00
abstract::Nontransformed cultures of vascular smooth muscle cells proliferate until they form a confluent sheet of cells. Subsequently, the cells become reorganized to form multicellular nodules that are loosely attached to the substrate. The formation of nodules is facilitated by the addition of medium conditioned by nodular c...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90095-7
更新日期:1983-06-01 00:00:00
abstract::All metazoan genomes encode multiple RAS GTPase activating proteins (RasGAPs) that negatively regulate the conserved RAS/MAPK signaling pathway. In mammals, several RasGAPs exhibit tumor suppressor activity by preventing excess RAS signal transduction. We have identified gap-3 as the to date missing Caenorhabditiseleg...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.08.026
更新日期:2008-11-15 00:00:00
abstract::The tadpole-type larva of Ciona has emerged as an intriguing model system for the study of neurodevelopment. The Ciona intestinalis connectome has been recently mapped, revealing the smallest central nervous system (CNS) known in any chordate, with only 177 neurons. This minimal CNS is highly reminiscent of larger CNS...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.09.023
更新日期:2019-04-15 00:00:00
abstract::Notch signaling is involved in a large range of developmental processes, and has been functionally implicated in body plan segmentation in two of the three diverse segmented taxa, the vertebrates and arthropods. Here we investigate expression of Notch, Delta, and hes gene homologues during larval and juvenile developm...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.04.015
更新日期:2008-08-01 00:00:00
abstract::Initiation of transcription mediated by RNA polymerase II requires a number of transcription factors among which TFIID is the major core promoter recognition factor. TFIID is composed of highly conserved factors which include the TATA-binding protein (TBP) and about 14 TBP-associated factors (TAFs). Recently, the comp...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.06.006
更新日期:2005-09-01 00:00:00
abstract::Early mammalian embryogenesis is controlled by mechanisms governing the balance between pluripotency and differentiation. The expression of early lineage-specific genes can vary significantly between species, with implications for developmental control and stem cell derivation. However, the mechanisms involved in patt...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.12.008
更新日期:2013-03-01 00:00:00
abstract::The members of the transforming growth factor-beta (TGF-beta) superfamily are secreted proteins that interact with cell-surface receptors to elicit signals that regulate a variety of biological processes during vertebrate embryogenesis. Alk2, also known as ActRIA, Tsk7L, and SKR1, encodes a type I TGF-beta family rece...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9378
更新日期:1999-09-15 00:00:00
abstract::The source of neurite outgrowth in explant cultures of normal adult Apteronotus spinal cord was examined. Explants which contained the central region of spinal cord, including ependyma, showed neurite outgrowth in culture. Explants which did not contain ependyma showed no neurite outgrowth. It is concluded that the ep...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90062-5
更新日期:1987-02-01 00:00:00
abstract::Regulation of peptide neurotransmitter metabolism was examined in dissociated cell cultures of neonatal rat sympathetic and sensory ganglia. Previous studies have shown that pineal gland conditioned medium (PCM) influences substance P (SP) and somatostatin (SS) metabolism in sympathetic neurons in vitro. The present s...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90061-7
更新日期:1984-11-01 00:00:00
abstract::The chick spinal cord can regenerate following injury until advanced developmental stages. It is conceivable that changes in stem/progenitor cell plasticity contribute to the loss of this capacity, which occurs around E13. We investigated the contribution of proliferation, phenotypic changes in radial glia progenitors...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.05.569
更新日期:2009-08-15 00:00:00
abstract::In the present study, we have investigated the role of tyrosine kinase activity during early retinal development in Xenopus laevis. The protein tyrosine kinase (PTK) inhibitors lavendustin A and genistein were used to determine the possible role of tyrosine kinase activity during retinal development in vivo and in vit...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.10.006
更新日期:2004-02-01 00:00:00
abstract::The ancestral state of animal gastrulation and its bearing for our understanding of bilaterian evolution still is one of the most controversially discussed topics in the field of evolutionary and developmental biology. One hypothesis, the so-called amphistomy scenario, suggests the presence of a slit-like blastopore i...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.04.014
更新日期:2017-07-01 00:00:00
abstract::Temporal and spatial aspects of floral determination in seedling terminal buds of the qualitative short-day plant Pharbitis nil were examined using a grafting assay. Floral determination in the terminal buds of 6-day-old P. nil seedlings is rapid; by 9 hr after the end of a 14-hr inductive dark period more than 50% of...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90268-n
更新日期:1990-02-01 00:00:00
abstract::Segmentation is a key step in embryonic development. Acting in all germ layers, it is responsible for the generation of antero-posterior asymmetries. Hox genes, with their diverse expression in individual segments, are fundamental players in the determination of different segmental fates. In vertebrates, Hox gene prod...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.04.016
更新日期:2010-07-01 00:00:00
abstract::Carbohydrate chains on the t12 mutant embryos were analyzed. No abnormalities of the synthesis of the carbohydrate chains were observed in the t12 homozygotes until the late morula stage, when radiolabeled carbohydrate chains were analyzed by Sephadex G-50 column chromatography. Furthermore, polarization of the Con A ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90309-0
更新日期:1988-08-01 00:00:00
abstract::The Caenorhabditis elegans EGL-17/FGF protein is involved in the gonadal signaling that guides the migrations of sex myoblasts (SMs). egl-17::GFP reporter constructs are expressed dynamically in vulval cell lineages. Expression in the primary vulval cells is correlated with the precise positioning of SMs. We have inve...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00033-2
更新日期:2003-05-01 00:00:00
abstract::Exposure of Xenopus animal pole explants to transforming growth factor beta 2 (TGF-beta 2) induced the sequential expression of muscle nicotinic acetylcholine receptor (AChRs) isoforms and their corresponding mRNAs in cells which normally give rise to ectoderm. Single channel recordings revealed two functional classes...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1318
更新日期:1994-11-01 00:00:00
abstract::The transforming growth factor-beta (TGF-beta) superfamily contains a variety of growth factors which all share common sequence elements and structural motifs. These proteins are known to exert a wide spectrum of biological responses on a large variety of cell types in both vertebrates and invertebrates. Many of them ...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:
更新日期:2002-10-15 00:00:00