Abstract：
:Cytoskeletal intermediate filaments were studied in muscular dysgenesis (mdg) and tetrodotoxin-treated inactive mouse embryo muscle cultures during myofibrillogenesis. Both muscular dysgenesis and tetrodotoxin-treated muscles are characterized in vitro by a total lack of contractile activity and an abnormal development of myofibrils. We studied the organization of the microtubule and intermediate filament networks with immunofluorescence, using anti-tubulin, anti-vimentin, and anti-desmin antibodies during normal and mdg/mdg myogenesis in vitro. Mdg/mdg myotubes present a heterogeneous microtubule network with scattered areas of decreased microtubule density. At the myoblast stage, cells expressed both vimentin and desmin. After fusion only desmin expression is revealed. In mutant myotubes the desmin network remains in a diffuse position and does not reorganize itself transversely, as it does during normal myogenesis. The absence of a mature organization of the desmin network in mdg/mdg myotubes is accompanied by a lack of organization of myofibrils. The role of muscle activity in the organization of myofibrils and desmin filaments was tested in two ways: (i) mdg/mdg myotubes were rendered active by coculturing with normal spinal cord cells, and (ii) normal myotubes were treated with tetrodotoxin (TTX) to suppress contractions. Mdg/mdg innervated myotubes showed cross-striated myofibrils, whereas desmin filaments remained diffuse. TTX-treated myotubes possessed disorganized myofibrils and a very unusual pattern of distribution of desmin: intensively stained desmin aggregates were superimposed upon the diffuse network. We conclude, on the basis of these results, that myofibrillar organization does not directly involve intermediate filaments but does need contractile activity.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Tassin AM,Pinçon-Raymond M,Paulin D,Rieger Fdoi
10.1016/0012-1606(88)90159-5subject
Has Abstractpub_date
1988-09-01 00:00:00pages
37-47issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(88)90159-5journal_volume
129pub_type
杂志文章abstract：:We describe the identification, biochemical characterisation, and mutation of a novel mouse gene: Sp5. Sp5 encodes a protein having a C-terminal C(2)H(2) zinc finger domain closely related to that of the transcription factor Sp1. In vitro, DNA binding studies show that it binds to the GC box, a DNA motif present in th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9878
更新日期：2000-11-15 00:00:00
abstract：:The protein tyrosine phosphatase PTP1, which mediates reversible phosphorylation on tyrosine, has been shown to play an important regulatory role during Dictyostelium development. Mutants lacking PTP1 develop more rapidly than normal, while strains that overexpress PTP1 display aberrant morphology. However, the signal...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0171
更新日期：2001-04-01 00:00:00
abstract：:Choriogenesis (eggshell formation) within the silkmoth Antheraea polyphemus proceeds in parallel for the two major subpopulations of follicle cells, diverging only during the very late period when aeropyle crown surface structures form in one region but not in the other. Correlated with their appearance is the synthes...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1301
更新日期：1993-11-01 00:00:00
abstract：:Notch activation inhibits neuronal differentiation during development of the nervous system; however, the dynamic role of Notch signaling in individual cell lineages remains poorly understood. We have characterized 3.4 kb 5'-regulatory sequence of a Notch target gene, her4, and used it to drive fluorescent gene expres...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.10.020
更新日期：2007-01-15 00:00:00
abstract：:Metazoan replication-dependent histone mRNAs accumulate to high levels during S phase as a result of an increase in the rate of histone gene transcription, pre-mRNA processing, and mRNA stability at the G1-S transition. However, relatively little is known about the contribution of these processes to histone expression...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.06.019
更新日期：2004-10-01 00:00:00
abstract：:Treatment of metaphase II-arrested hamster eggs with activators of protein kinase C has been reported to promote resumption of the cell cycle, second polar body emission, and pronucleus formation (G.I. Gallicano, S.M. Schwarz, R.W. McGaughey, and D.G. Capco, 1993, Dev. Biol. 156, 94-106). In contrast, we have not obse...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1233
更新日期：1995-08-01 00:00:00
abstract：:Specification of the primordial germ cells (PGCs) is essential for sexually reproducing animals. Although the mechanisms of PGC specification are diverse between organisms, the RNA binding protein Nanos is consistently required in the germ line in all species tested. How Nanos is selectively expressed in the germ line...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.04.010
更新日期：2019-08-01 00:00:00
abstract：:The subdivision of the developing field by region-specific expression of genes encoding transcription factors is an essential step during appendage development in arthropod and vertebrates. In Drosophila leg development, the distal-most region (pretarsus) is specified by the expression of homeobox genes, aristaless an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.12.005
更新日期：2005-03-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1212
更新日期：1995-08-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9549
更新日期：2000-01-15 00:00:00
abstract：:Mutations in the XNP/ATR-X gene cause several X-linked mental retardation syndromes in humans. The XNP/ATR-X gene encodes a DNA-helicase belonging to the SNF2 family. It has been proposed that XNP/ATR-X might be involved in chromatin remodelling. The lack of a mouse model for the ATR-X syndrome has, however, hampered ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.10.014
更新日期：2005-02-01 00:00:00
abstract：:The nervous system development of the sea cucumber Stichopus japonicus was investigated to explore the development of the bilateral larval nervous system into the pentaradial adult form typical of echinoderms. The first nerve cells were detected in the apical region of epidermis in the late gastrula. In the auriculari...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.12.038
更新日期：2006-04-01 00:00:00
abstract：:P19 embryonal carcinoma cells are multipotential stem cells that differentiate into striated muscle as well as some other cell types when aggregated and exposed to dimethyl sulfoxide (DMSO). Immunofluorescence experiments using monospecific antibodies indicated that the majority of muscle cells were mononucleate and c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90202-t
更新日期：1990-04-01 00:00:00
abstract：:The fertilized ascidian egg is thought to be comprised of distinct regions of tissue-specific cytoplasmic determinants. This idea was tested by bisecting fertilized eggs into egg fragments and culturing them until the unoperated controls developed into larvae. Fertilized eggs were bisected using a microsurgical method...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90178-9
更新日期：1988-09-01 00:00:00
abstract：:Animal development is controlled by gene regulation networks that are composed of sequence-specific transcription factors (TF) and cell signaling molecules (ST). Although housekeeping genes have been reported to show clustering in the animal genomes, whether the genes comprising a given regulatory network are physical...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.01.009
更新日期：2008-04-15 00:00:00
abstract：:The mesoderm of the sea urchin embryo conventionally is divided into two populations of cells; the primary mesenchyme cells (PMCs), which produce the larval skeleton, and the secondary mesenchyme cells (SMCs), which differentiate into a variety of cell types but do not participate in skeletogenesis. In this study we e...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90220-5
更新日期：1988-02-01 00:00:00
abstract：:BMP-2 and BMP-4 are known to be involved in the early events which specify the cardiac lineage. Their later patterns of expression in the developing mouse and chick heart, in the myocardium overlying the atrioventricular canal (AV) and outflow tract (OFT) cushions, also suggest that they may play a role in valvoseptal...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0291
更新日期：2001-07-01 00:00:00
abstract：:Vimentin expression in the lens is striking due to the reported mesenchymal preference of vimentin and the epithelial origin of the lens. The amount of chicken vimentin mRNA levels determined by Northern blot analysis increased 3-fold from 7 to 14 days of embryonic lens development and then decreased 10-fold at 16 day...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90278-q
更新日期：1990-05-01 00:00:00
abstract：:Analysis of 262 transgenic mouse pedigrees suggests that about 30% of the mice produced by microinjection of plasmids into pronuclei are mosaic in the germline. This implies that in these lines integration of the foreign DNA occurred after the first round of chromosomal DNA replication. In mosaics resulting from delay...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90068-0
更新日期：1986-11-01 00:00:00
abstract：:Fetomodulin (FM) was previously shown to be a surface marker protein of parietal endoderm by in vitro differentiation of F9 embryonal carcinoma cells and by immunohistochemistry of in vivo embryos. BALB/3T3 and sarcoma S180 cells of the mouse were also shown to possess a protein which was indistinguishable from FM by ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90058-q
更新日期：1990-07-01 00:00:00
abstract：:During development of the central nervous system (CNS), commissural axons grow toward the ventral midline. After crossing the floor plate, they abruptly change their trajectory from the circumferential to the longitudinal axis. The contacts between the commissural axons and the floor plate cells are involved in this a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.05.034
更新日期：2004-09-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8592
更新日期：1997-06-15 00:00:00
abstract：:We describe the characterization and a functional analysis in Xenopus development of RalB, a small G protein. RalB RNA and protein are detectable during oogenesis and early development, but the gene is expressed only weakly in adult tissues. The RalB transcripts are processed by poly(A) extension during oocyte maturat...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9254
更新日期：1999-05-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.05.662
更新日期：2011-09-01 00:00:00
abstract：:The N-ethyl-N-nitrosourea (ENU) ←forward genetic screen is a useful tool for the unbiased discovery of novel mechanisms regulating developmental processes. We recovered the dorothy mutation in such a screen designed to recover recessive mutations affecting craniofacial development in the mouse. Dorothy embryos die pre...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.10.039
更新日期：2020-02-15 00:00:00
abstract：:During epithelial development cells become polarized along their apical-basal axis and some epithelia also exhibit polarity in the plane of the tissue. Mutations in the gene encoding a Drosophila Pak family serine/threonine kinase, dPak, disrupt the follicular epithelium that covers developing egg chambers during ooge...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.02.034
更新日期：2007-05-15 00:00:00
abstract：:The serine/threonine kinase LKB1 is a master kinase that regulates a number of critical events such as cell transformation, polarization, development, stress response, and energy metabolism in metazoa. After multiple unsuccessful attempts of generating Dictyostelium lkb1-null cells, an RNAi-based knockdown approach pr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.10.006
更新日期：2011-12-15 00:00:00
abstract：:The Drosophila brain is generated by a complex series of morphogenetic movements. To better understand brain development and to provide a guide for experimental manipulation of brain progenitors, we created a fate map using photoactivated gene expression to mark cells originating within specific mitotic domains and ti...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00220-3
更新日期：2003-08-01 00:00:00
abstract：:Egg yolk phosvitin is one of the most highly phosphorylated extracellular matrix proteins known in nature with unique physico-chemical properties deemed to be critical during ex-vivo egg embryo development. We have utilized our unique live mouse calvarial bone organ culture models under conditions which dissociates th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.06.005
更新日期：2013-09-01 00:00:00
abstract：:Cardiomyocyte differentiation overall has been analyzed in vivo and in vitro at the molecular level by homologous recombination, gene mutation studies, and by transgenics; however, the roles of many signal transduction mechanisms that drive this differentiation process are still not fully understood. One set of signal...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00080-5
更新日期：2003-03-15 00:00:00
