Abstract：
:Adherent cultures of E10.5 rat neuroepithelial cells (NEP cells) from the caudal neural tube require FGF (fibroblast growth factor) and CEE (chick embryo extract) to proliferate and maintain an undifferentiated phenotype in culture. Epidermal growth factor (EGF) does not support E10.5 NEP cells in adherent culture and NEP cells do not form EGF-dependent neurospheres. NEP cells, however, can be grown as FGF-dependent neurospheres. NEP cells express nestin and lack all lineage-specific markers for neuronal and glial sublineages, retain their pleuripotent character over multiple passages, and can differentiate into neurons, astrocytes, and oligodendrocytes when plated on laminin in the absence of CEE. In clonal culture, NEP cells undergo self-renewal and generate colonies that vary in size from single cells to several thousand cells. With the exception of a few single-cell clones, all other NEP-derived clones contain more than one identified phenotype, with over 40% of the colonies containing A2B5, beta-111 tubulin, and GFAP-immunoreactive cells. Thus, NEP cells are multipotent and capable of generating multiple neural derivatives. NEP cells also differentiate into motoneurons immunoreactive for choline acetyl transferase (ChAT) and the low-affinity neurotrophin receptor (p75) in both mass and clonal culture. Double labeling of clones for ChAT and glial, neuronal, or oligodendrocytic lineage markers shows that motoneurons always arose in mixed cultures with other differentiated cells. Thus, NEP cells represent a common progenitor for motoneurons and other spinal cord cells. The relationship of NEP cells with other neural stem cells is discussed.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Kalyani A,Hobson K,Rao MSdoi
10.1006/dbio.1997.8592subject
Has Abstractpub_date
1997-06-15 00:00:00pages
202-23issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(97)98592-4journal_volume
186pub_type
杂志文章abstract：:The desmin gene encodes an intermediate filament protein that is present in skeletal, cardiac, and smooth muscle cells. This study shows that the 4-kb upstream region of the murine desmin promoter directs expression of a lacZ reporter gene throughout the heart from E7.5 and in skeletal muscle and vascular smooth muscl...
journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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journal_title：Developmental biology
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0717
更新日期：2002-08-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9659
更新日期：2000-06-15 00:00:00
abstract：UNLABELLED:Notch signaling plays an acknowledged role in bile-duct development, but its involvement in cholangiocyte-fate determination remains incompletely understood. We investigated the effects of early Notch2 deletion in Notch2(fl/fl)/Alfp-Cre(tg/-) ("Notch2-cKO") and Notch2(fl/fl)/Alfp-Cre(-/-) ("control") mice. F...
journal_title：Developmental biology
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doi：10.1016/j.ydbio.2014.10.002
更新日期：2014-12-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.01.023
更新日期：2011-04-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8704
更新日期：1997-10-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90340-8
更新日期：1983-07-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.04.018
更新日期：2009-07-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.12.029
更新日期：2008-03-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90075-e
更新日期：1991-02-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1990-02-01 00:00:00
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更新日期：2008-09-15 00:00:00
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更新日期：2014-10-01 00:00:00
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1986-10-01 00:00:00
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更新日期：2012-08-01 00:00:00
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更新日期：2005-02-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2013-10-01 00:00:00
abstract：:We have examined the interfaces between protein and lipid sulfation, the response of progenitor cells to specific growth factors, and developmental lineage progression. The experimental system used is the oligodendrocyte (OL), the cell responsible for myelination of axons in the central nervous system. The ability to ...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1994-04-01 00:00:00
