Abstract：
:Three myogenic clonal cell lines were isolated from C3H 10T1/2 C18 cells (10T1/2) treated with 5-azacytidine (5-aza-CR). These lines reproducibly underwent fusion at confluence into functional myotubes capable of contracting in response to acetylcholine. The degree of fusion could be increased two- to threefold if the cells were grown on gelatin-coated dishes. All of the cell lines lost some of their myogenic potential after repeated passaging and the percentage of colonies capable of forming muscle was not increased by permissive media containing 2% horse serum. The 10T1/2 cells expressed only the BB form of creatine phosphokinase but all of the myogenic clones expressed additionally the MM and MB forms of the isozyme after fusion. The overall genomic level of 5-methylcytosine was decreased in some but not all of the cell clones tested. Comparisons between the 10T1/2 cells which never form muscle without 5-aza-CR treatment and clonal derivatives of committed cell types might be of value in understanding the molecular basis of the commitment process.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Liu L,Harrington M,Jones PAdoi
10.1016/0012-1606(86)90302-7subject
Has Abstractpub_date
1986-10-01 00:00:00pages
331-6issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(86)90302-7journal_volume
117pub_type
杂志文章abstract：:Kidney development is a complex, little understood process based on inductive interactions and intricate epithelial and mesenchymal morphogenesis. Here, we report the use of subtractive hybridization to clone cDNAs expressed in early nephrogenesis. cDNA made from E14.5 mouse kidney was hybridized with adult mouse live...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1139
更新日期：1995-05-01 00:00:00
abstract：:Maize leaves are divided into repeated longitudinal units consisting of vascular tissue, bundle sheath (BS), and mesophyll (M) cells. We have carried out a cell lineage analysis of these cell types using six spontaneous striping mutants of maize. We show that certain cell division patterns are preferentially utilized,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90304-7
更新日期：1989-05-01 00:00:00
abstract：:The gastrointestinal (GI) tract of metazoans is lined by a series of regionally distinct epithelia. To maintain structure and function of the GI tract, regionally diversified differentiation of somatic stem cell (SC) lineages is critical. The adult Drosophila midgut provides an accessible model to study SC regulation ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.08.027
更新日期：2016-11-15 00:00:00
abstract：:The mab-21 gene was first identified because of its requirement for ray identity specification in Caenorhabditis elegans. It is now known to constitute a family of genes that are highly conserved from vertebrates to invertebrates, and two homologues Mab21l1 and Mab21l2 have been identified in many species. Here we des...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.07.016
更新日期：2004-10-15 00:00:00
abstract：:Directed cell movement is integral to both embryogenesis and hematopoiesis. In the adult, the chemokine family of secreted proteins signals migration of hematopoietic cells through G-coupled chemokine receptors. We detected embryonic expression of chemokine receptor messages by RT-PCR with degenerate primers at embryo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9405
更新日期：1999-09-15 00:00:00
abstract：:It is known that secreted proteins from the anterior lateral endoderm, FGF8 and BMP2, are involved in mesodermal cardiac differentiation, which determines the first cardiac field, defined by the expression of the earliest specific cardiac markers Nkx-2.5 and Gata4. However, the molecular mechanisms responsible for ear...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.07.007
更新日期：2015-10-01 00:00:00
abstract：:A series of chimeras was generated by injecting single normal, parthenogenetic, or androgenetic blastomeres carrying transgenic markers under the zona pellucida of nontransgenic eight-cell embryos. These chimeras were cultured to the blastocyst stage and sectioned, and the transgenic component was detected by in situ ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(89)80028-4
更新日期：1989-02-01 00:00:00
abstract：:Full-grown Xenopus oocytes, Stage VI (1200-1300 microns), undergo meiotic maturation when exposed to progesterone. Smaller stage IV (800 microns) and stage V (1000 microns) oocytes remain in prophase arrest when exposed to this steroid. The larger stage VI oocytes undergo an intracellular alkalization from 7.2 to 7.6,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90288-4
更新日期：1984-10-01 00:00:00
abstract：:A fundamental feature of cartilage differentiation in the developing limb is the formation of a prechondrogenic cell condensation. An apparently similar process of prechondrogenic cell aggregation occurs in micromass cultures of limb bud mesenchyme with the formation of cellular aggregates which often differentiate in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90314-9
更新日期：1987-08-01 00:00:00
abstract：:Chick sensory neurons grow to their correct targets in the hindlimb from the outset during normal development and following various experimental manipulations. This may result not because sensory neurons respond to specific limb-derived cues, but because they interact in some way with motoneurons which are responsive ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90023-0
更新日期：1986-12-01 00:00:00
abstract：:Spermatogonial stem cells (SSCs) must balance self-renewal with production of transit-amplifying progenitors that differentiate in response to retinoic acid (RA) before entering meiosis. This self-renewal vs. differentiation spermatogonial fate decision is critical for maintaining tissue homeostasis, as imbalances cau...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.08.004
更新日期：2015-11-01 00:00:00
abstract：:Lipids are essential for cellular function as sources of fuel, critical signaling molecules and membrane components. Deficiencies in lipid processing and transport underlie many metabolic diseases. To better understand metabolic function as it relates to disease etiology, a whole animal approach is advantageous, one i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.09.010
更新日期：2011-12-15 00:00:00
abstract：:Changes in the intracellular free calcium levels in the cortical region of cleaving Xenopus eggs were examined using semi-synthetic aequorin. We detected periodic oscillations in the free calcium levels accompanying the cleavage cycle as early as the first cleavage onward. The calcium levels began to increase at early...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1325
更新日期：1993-12-01 00:00:00
abstract：:Replication-dependent histone genes in the mouse and Xenopus share a common regulatory element within the protein-encoding sequence called the CRAS alpha element (coding region activating sequence alpha) which has been shown to mediate normal expression in vivo and to interact with nuclear factors in vitro in a cell c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.8459
更新日期：1997-02-01 00:00:00
abstract：:Immunohistochemical analysis of myosin heavy chain (MHC) isoform expression in perinatal and adult rat diaphragm muscles was performed with antibodies which permitted the identification of all known MHC isoforms found in typical rat muscles. Isoform switching, leading to the emergence of the adult phenotype, was more ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90473-g
更新日期：1991-03-01 00:00:00
abstract：:Urodele amphibians can regenerate their limbs. During limb regeneration, dermal fibroblasts are transformed into undifferentiated cells called blastema cells. These dermis-blastema cells show multipotency. Such so-called endogenous reprogramming of cell differentiation is one of the main targets of amphibian limb rege...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.05.010
更新日期：2013-09-01 00:00:00
abstract：:Antibodies to nonerythroid alpha spectrin (p 230) were used to study the distribution of this polypeptide in mouse germ cells, zygote, and early embryonic cells. In the primordial germ cells, fetal oocytes, and spermatogonia, spectrin was found predominantly in the form of a narrow condensed subplasmalemmal band, as i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90389-1
更新日期：1986-03-01 00:00:00
abstract：:The Mesp bHLH genes play a conserved role during segmental patterning of the mesoderm in the vertebrate embryo by specifying segmental boundaries and anteroposterior (A-P) segmental polarity. Here we use a xenotransgenic approach to compare the transcriptional enhancers that drive expression of the Mesp genes within s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.12.038
更新日期：2008-03-15 00:00:00
abstract：:We have studied the differentiation of the oocyte vitelline coat (VC) and jelly coat (JC) of the starfish, Astropecten aurantiacus. The precursor material of both envelopes is secreted by the oocyte while the follicle cells do not appear to participate in the secretory process. The first indication of differentiation ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90296-8
更新日期：1983-10-01 00:00:00
abstract：:Gene network analysis requires computationally based models which represent the functional architecture of regulatory interactions, and which provide directly testable predictions. The type of model that is useful is constrained by the particular features of developmentally active cis-regulatory systems. These systems...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1006/dbio.2002.0617
更新日期：2002-06-01 00:00:00
abstract：:Pax3/7 paired homeodomain transcription factors are important markers of muscle stem cells. Pax3 is required upstream of myod for lateral dermomyotomal cells in the amniote somite to form particular muscle cells. Later Pax3/7-dependent cells generate satellite cells and most body muscle. Here we analyse early myogenes...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.10.009
更新日期：2007-02-15 00:00:00
abstract：:Interstitial cell migration was characterized in Hydra vulgaris (formerly H. attenuata) and the effects of axial position and tissue injury on migration were investigated. Migrating cells were labeled with the thymidine analog, bromodeoxyuridine, and grafted into unlabeled host polyps. Alternatively, cells were labele...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90177-k
更新日期：1990-03-01 00:00:00
abstract：:We have cloned a member of the fork head/HNF-3 family of transcription factors from the nematode Caenorhabditis elegans. Within the predicted DNA binding domain, this gene, called Ce-fkh-1, is 75-78% identical to the Drosophila fork head and rat liver HNF-3 alpha, beta, and gamma genes. Ce-fkh-1 mRNA is highly enriche...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0219
更新日期：1996-09-15 00:00:00
abstract：:The task of the visual system is to translate light into neuronal encoded information. This translation of photons into neuronal signals is achieved by photoreceptor neurons (PRs), specialized sensory neurons, located in the eye. Upon perception of light the PRs will send a signal to target neurons, which represent a ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.07.006
更新日期：2011-10-01 00:00:00
abstract：:Eight series of mouse chimeras were produced by aggregating 8-cell embryos that differed at the Gpi-1s locus, encoding glucose phosphate isomerase (GPI-1). Chimeric blastocysts (Gpi-1sa/Gpi-1sa <--> Gpi-1sb/Gpi-1sb) were transferred to pseudopregnant females, which produced only GPI-1C enzyme. Quantitative electrophor...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1062
更新日期：1995-03-01 00:00:00
abstract：:Horseradish peroxidase (HRP) was used as an intracellular lineage tracer in two experiments designed to reveal the sites of origin of cells that formed the duplicate embryo which developed in relation to an organizer grafted in the ventral marginal zone (VMZ) of Xenopus laevis embryos. In the first experiment a dorsal...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90180-5
更新日期：1984-03-01 00:00:00
abstract：:It has been difficult to examine the role of TGF-ß in post-natal tooth development due to perinatal lethality in many of the signaling deficient mouse models. To address the role of Tgfbr2 in postnatal tooth development, we generated a mouse in which Tgfbr2 was deleted in odontoblast- and bone-producing mesenchyme. Os...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.08.003
更新日期：2013-10-01 00:00:00
abstract：:The development of the action potential and responses to neurotransmitters have been described for a population of embryonic spinal neurons developing in vivo. A comparable pattern is seen for spinal neurons developing in dissociated cell culture. The impulse appears very early in this developmental sequence, and the ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90065-4
更新日期：1984-11-01 00:00:00
abstract：:The enteric nervous system (ENS) of the larval moth Manduca sexta consists of two small ganglia and several nerve networks that lie superficially along the alimentary tract. Within this system are approximately 600 neurons that exhibit a spectrum of biochemical and morphological characteristics and that express these ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(89)80039-9
更新日期：1989-01-01 00:00:00
abstract：:The uterus undergoes a series of dramatic changes in response to an implanting conceptus that, in some mammalian species, includes differentiation of the endometrial stroma into decidual tissue. This process, called decidualization, can be induced artificially in rodents indicating that the conceptus may not be essent...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.03.006
更新日期：2006-06-15 00:00:00
