Abstract：
:Lipids are essential for cellular function as sources of fuel, critical signaling molecules and membrane components. Deficiencies in lipid processing and transport underlie many metabolic diseases. To better understand metabolic function as it relates to disease etiology, a whole animal approach is advantageous, one in which multiple organs and cell types can be assessed simultaneously in vivo. Towards this end, we have developed an assay to visualize fatty acid (FA) metabolism in larval zebrafish (Danio rerio). The method utilizes egg yolk liposomes to deliver different chain length FA analogs (BODIPY-FL) to six day-old larvae. Following liposome incubation, larvae accumulate the analogs throughout their digestive organs, providing a comprehensive readout of organ structure and physiology. Using this assay we have observed that different chain length FAs are differentially transported and metabolized by the larval digestive system. We show that this assay can also reveal structural and metabolic defects in digestive mutants. Because this labeling technique can be used to investigate digestive organ morphology and function, we foresee its application in diverse studies of organ development and physiology.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Carten JD,Bradford MK,Farber SAdoi
10.1016/j.ydbio.2011.09.010subject
Has Abstractpub_date
2011-12-15 00:00:00pages
276-85issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(11)01254-1journal_volume
360pub_type
杂志文章abstract：:We describe mesendoderm morphogenesis during gastrulation in the frog Xenopus laevis and investigate the mechanics of these movements with tissue explants. When a dorsal marginal zone explant is plated onto fibronectin, the mesendoderm moves away from the dorsal axial tissues as an intact sheet. Mesendodermal cells wi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0537
更新日期：2002-02-15 00:00:00
abstract：:Oocyte maturation in mouse is associated with a dramatic reorganisation of the endoplasmic reticulum (ER) from a network of cytoplasmic accumulations in the germinal vesicle-stage oocyte (GV) to a network of distinctive cortical clusters in the metaphase II egg (MII). Multiple lines of evidence suggest that this redis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.02.006
更新日期：2007-05-01 00:00:00
abstract：:DNA replication and its connection to M phase restraint are studied extensively at the level of single cells but rarely in the context of a developing animal. C. elegans lin-6 mutants lack DNA synthesis in postembryonic somatic cell lineages, while entry into mitosis continues. These mutants grow slowly and either die...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.12.009
更新日期：2011-02-15 00:00:00
abstract：:The levels of al-1 and al-2 transcripts change dramatically in response to light and development during the formation of Neurospora crassa asexual spores (conidia). al-1 and al-2 mRNAs accumulate throughout conidiation irrespective of lighting conditions initially at low levels. As conidiation proceeds, two increases ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1129
更新日期：1995-05-01 00:00:00
abstract：:Acquisition of limb-forming ability by discrete regions of the lateral plate of the chick embryo is thought to depend on a signaling cascade moving sequentially from the area of Hensen's node to the somitic mesoderm, the intermediate mesoderm, and then to the prospective limb-forming regions of the lateral plate (Step...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8646
更新日期：1997-08-15 00:00:00
abstract：:Nonmuscle myosin-II is a key motor protein that drives cell shape change and cell movement. Here, we analyze the function of nonmuscle myosin-II during Drosophila embryonic myogenesis. We find that nonmuscle myosin-II and the adhesion molecule, PS2 integrin, colocalize at the developing muscle termini. In the paradigm...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0452
更新日期：2001-11-15 00:00:00
abstract：:Stimulation of epithelial cell cycling by the neuropeptide head activator was analyzed in Hydra magnipapillata and compared with the action of head activator on bud formation and tentacle formation during head regeneration. The results obtained indicate that head activator treatment stimulates epithelial cell division...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.8491
更新日期：1997-03-01 00:00:00
abstract：:In many organisms, like Caenorhabditis elegans and Drosophila melanogaster, establishment of spatial patterns and definition of cell fate are driven by the segregation of determinants in response to spatial cues, as early as oogenesis or fertilization. In these organisms, a family of conserved proteins, the PAR protei...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.03.001
更新日期：2005-06-15 00:00:00
abstract：:The organizer is an essential signaling center required for axial formation during vertebrate embryonic development. In the basal chordate amphioxus, the dorsal blastopore lip of the gastrula has been proposed to be homologous to the vertebrate organizer. Lefty is one of the first genes to be expressed in the organize...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.08.006
更新日期：2019-12-01 00:00:00
abstract：:We have investigated the ability of growing dictyate oocytes and early preimplantation embryos of the mouse to process extrachromosomal DNA molecules with free ends by intranuclearly microinjecting DNA fragments containing a region of homology of various extent at either the 5' or 3' terminus. Homologous recombination...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0199
更新日期：2001-05-01 00:00:00
abstract：:Wnt signaling pathways have been demonstrated to play important roles in controlling tissue patterning and cell proliferation. In the gastrointestinal tract, mutations that lead to activation of the canonical Wnt pathway through beta-catenin result in familial and sporadic colon cancers. The downstream transcription f...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00185-4
更新日期：2003-07-15 00:00:00
abstract：:The regulation of Sertoli cell transferrin and sulfated glycoprotein-2 (SGP-2) mRNA levels by vitamin A was studied in vitamin A deficient rats. Vitamin A deficiency differentially affected the levels of these mRNAs. Sertoli cells from vitamin A deficient rats contained 3-fold less transferrin mRNA and 1.8-fold more S...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90167-9
更新日期：1987-06-01 00:00:00
abstract：:One way to better understand the molecular mechanisms involved in the construction of a nervous system is to identify the downstream effectors of major regulatory proteins. We previously showed that Engrailed (EN) and Gooseberry-Neuro (GsbN) transcription factors act in partnership to drive the formation of posterior ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.10.018
更新日期：2017-12-15 00:00:00
abstract：:The sensory neurons of the Drosophila wing differentiate during the initial stages of metamorphosis, appearing in the imaginal wing disc as it everts and flattens. These identifiable neurons arise in a stereotyped sequence, and lay down a specific pattern of axon bundles which travel proximally to the CNS. In several ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90044-2
更新日期：1985-04-01 00:00:00
abstract：:Fertilization triggers activation of Src-family kinases in eggs of various species including marine invertebrates and lower vertebrates. While immunofluorescence studies have localized Src-family kinases to the plasma membrane or cortical cytoplasm, no information is available regarding the extent to which these kinas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.03.041
更新日期：2006-07-15 00:00:00
abstract：:Neurons and glia are produced in stereotyped patterns after neuroblast cell division during development of the Drosophila central nervous system. The first cell division of thoracic neuroblast 6-4 (NB6-4T) is asymmetric, giving rise to a glial precursor cell and a neuronal precursor cell. In contrast, abdominal NB6-4 ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9727
更新日期：2000-06-15 00:00:00
abstract：:Fetomodulin (FM) was previously shown to be a surface marker protein of parietal endoderm by in vitro differentiation of F9 embryonal carcinoma cells and by immunohistochemistry of in vivo embryos. BALB/3T3 and sarcoma S180 cells of the mouse were also shown to possess a protein which was indistinguishable from FM by ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90058-q
更新日期：1990-07-01 00:00:00
abstract：:Msx1 and Msx2 are highly conserved, Nk-related homeodomain transcription factors that are essential for a variety of tissue-tissue interactions during vertebrate organogenesis. Here we show that combined deficiencies of Msx1 and Msx2 cause conotruncal anomalies associated with malalignment of the cardiac outflow tract...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.05.037
更新日期：2007-08-15 00:00:00
abstract：:Prophase-arrested oocytes of Ruditapes philippinarum are triggered to undergo germinal vesicle breakdown under the influence of the neurohormone serotonin (5HT) and then arrest in metaphase 1. Our data show that these oocytes possess a single class of original 5HT receptors. Their binding parameters have been determin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1222
更新日期：1994-08-01 00:00:00
abstract：:CD9 is a membrane protein belonging to the tetraspanin family. Despite CD9's broad tissue distribution, the only abnormality observed in CD9-deficient mice was infertility of females, which was responsible for a defect in the sperm-egg fusion process. However, the function of CD9 in sperm-egg fusion is not clear at al...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0694
更新日期：2002-07-15 00:00:00
abstract：:We describe the identification, biochemical characterisation, and mutation of a novel mouse gene: Sp5. Sp5 encodes a protein having a C-terminal C(2)H(2) zinc finger domain closely related to that of the transcription factor Sp1. In vitro, DNA binding studies show that it binds to the GC box, a DNA motif present in th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9878
更新日期：2000-11-15 00:00:00
abstract：:Tube formation is a widespread process during organogenesis. Specific cellular behaviors participate in the invagination of epithelial monolayers that form tubes. However, little is known about the evolutionary mechanisms of cell assembly into tubes during development. In Caenorhabditis elegans, the detailed step-to-s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.10.029
更新日期：2004-02-15 00:00:00
abstract：:Stem cells are essential for the regeneration and homeostasis of many organs, such as tooth, hair, skin, and intestine. Although human tooth regeneration is limited, a number of animals have evolved continuously growing teeth that provide models of stem cell-based organ renewal. A well-studied model is the mouse incis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.03.012
更新日期：2012-06-15 00:00:00
abstract：:We have investigated oocyte development in Hydra vulgaris, a member of one of the oldest metazoan phyla. We show that oocyte determination involves a mechanism that establishes a subset of precursor interstitial cells competent to differentiate into oocytes. The oocyte is singled out from this subset and the competenc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9790
更新日期：2000-08-15 00:00:00
abstract：:Cerebral and spinal vascular systems are organized individually, and they then conjugate at their border, through the integration of basilar artery and vertebral arteries. Zebrafish (Danio rerio) is an ideal organism for studying early vascular development, and the precise procedure of cranial and truncal vascular for...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.07.015
更新日期：2015-10-01 00:00:00
abstract：:Development of the craniofacial structures requires the precise differentiation of cranial neural crest cells into osteoblasts or chondrocytes. Here, we explore the epigenetic and non-epigenetic mechanisms that are required for the development of craniofacial chondrocytes. We previously demonstrated that the acetyltra...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.05.006
更新日期：2020-08-01 00:00:00
abstract：:Heparan sulfate proteoglycans (HSPGs) regulate a number of major developmental processes, but their roles in synovial joint formation remain unknown. Here we created conditional mouse embryo mutants lacking Ext1 in developing joints by mating Ext1(f/f) and Gdf5-Cre mice. Ext1 encodes a subunit of the Ext1/Ext2 Golgi-a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.12.022
更新日期：2011-03-01 00:00:00
abstract：:A protein complex consisting of Mago Nashi and Tsunagi/Y14 is required to establish the major body axes and for the localization of primordial germ cell determinants during Drosophila melanogaster oogenesis. The Mago Nashi:Tsunagi/Y14 heterodimer also serves as the core of the exon junction complex (EJC), a multiprote...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.06.007
更新日期：2007-08-15 00:00:00
abstract：:Islet1 (Isl1) is a LIM homedomain protein that plays a pivotal role in cardiac progenitors of the second heart field. Here, lineage studies with an inducible isl1-cre demonstrated that most Isl1 progenitors have migrated into the heart by E9. Although Isl1 expression is downregulated in most cardiac progenitors as the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.12.048
更新日期：2007-04-01 00:00:00
abstract：:Immunocytochemical studies were performed to determine if denervated rodent iris produces nerve growth factor (NGF) in a form chemically similar to that of the 7S NGF complex in mouse submandibular glands. Antisera to the alpha, beta, and gamma subunits of 7S NGF were raised in rabbits and characterized on immunoblots...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90201-0
更新日期：1986-04-01 00:00:00
