Abstract:
:Prophase-arrested oocytes of Ruditapes philippinarum are triggered to undergo germinal vesicle breakdown under the influence of the neurohormone serotonin (5HT) and then arrest in metaphase 1. Our data show that these oocytes possess a single class of original 5HT receptors. Their binding parameters have been determined on semipurified membrane preparations incubated with [3H]5HT. No significant differences were observed when comparing 5HT-competent and -incompetent batches as well as prophase- or metaphase-arrested oocytes. Specific experiments including incubation with mastoparan or mas 7, GTP iontophoresis, and IP3 quantification strongly suggest that these receptors must be coupled with G-proteins to be functional. Peak change in IP3 mass occurs at 3 min and is likely to trigger the 5HT-dependent Ca2+ transient that begins at this time. In metaphase-arrested oocytes, binding of 5HT to its receptors no longer produces a Ca2+ surger. This is likely to result from a negative retrocontrol loop which would involve kinase C and exert its effect upstream of the Ca2+ surge. Indeed, the phorbol ester PMA proved able to reduce the Ca2+ response and to block 5HT action when applied during the first 3 min corresponding to the hormone-dependent period. Such an inhibition was reversed in the presence of 5 microM of the C kinase inhibitor GF109203X and could be bypassed by ionophore, ammonia, and thapsigargin, which trigger a receptor-independent Ca2+ surge.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Gobet I,Durocher Y,Leclerc C,Moreau M,Guerrier Pdoi
10.1006/dbio.1994.1222subject
Has Abstractpub_date
1994-08-01 00:00:00pages
540-9issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(84)71222-Xjournal_volume
164pub_type
杂志文章abstract::We have cloned a partial cDNA of chicken glial cell line-derived neurotrophic factor (GDNF) and systematically examined its expression pattern as well as that of GDNF-binding components (GDNF family receptor alpha-1 and 2: GFRalpha-1 and 2) and a common signal transduction receptor (c-ret protooncogene: RET) during ve...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9543
更新日期:2000-01-01 00:00:00
abstract::Normal growth and morphogenesis of the cranial vault reflect a balance between cell proliferation in the sutures and osteogenesis at the margins of the cranial bones. In the clinical condition craniosynostosis, the sutures fuse prematurely as a result of precocious osteogenic differentiation and craniofacial malformat...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0533
更新日期:2002-03-01 00:00:00
abstract::While Wnt signaling is known to be involved in early steps of neural crest development, the mechanism remains unclear. Because Wnt signaling is able to posteriorize anterior neural tissues, neural crest induction by Wnts has been proposed to be an indirect consequence of posteriorization of neural tissues rather than ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.12.016
更新日期:2005-03-01 00:00:00
abstract::In the early Drosophila embryo, Bone morphogenetic protein (BMP) activity is positively and negatively regulated by the BMP-binding proteins Short gastrulation (Sog) and Twisted gastrulation (Tsg). We show here that a similar mechanism operates during crossvein formation, utilizing Sog and a new member of the tsg gene...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.02.029
更新日期:2005-06-01 00:00:00
abstract::Rhodopsin is essential for normal photoreceptor development in Drosophila (O'Tousa et al., 1989; Leonard et al., 1992; Kumar and Ready, 1995) and in mice (Humphries et al., 1997). Here we report studies in which a rhodopsin transgene is expressed at restricted stages during the development of Drosophila photoreceptors...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8636
更新日期:1997-08-01 00:00:00
abstract::The adult fungiform taste papilla is a complex of specialized cell types residing in the stratified squamous tongue epithelium. This unique sensory organ includes taste buds, papilla epithelium and lateral walls that extend into underlying connective tissue to surround a core of lamina propria cells. Fungiform papilla...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.07.022
更新日期:2013-10-01 00:00:00
abstract::This first study of the onset of spermatogenesis in the sea urchin, Strongylocentrotus purpuratus, was undertaken using individuals reared in the laboratory. Spermatogenesis commences about 11-12 months after metamorphosis in these animals. Bindin message accumulates in late spermatocytes and early spermatids which li...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90149-d
更新日期:1990-11-01 00:00:00
abstract::Quantification of GFP reporter gene expression at single cell level in living sea urchin embryos can now be accomplished by a new method of confocal laser scanning microscopy (CLSM). Eggs injected with a tissue-specific GFP reporter DNA construct were grown to gastrula stage and their fluorescence recorded as a series...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.06.016
更新日期:2006-11-15 00:00:00
abstract::Metamorphosis in Drosophila melanogaster is orchestrated by the steroid hormone ecdysone, which triggers a cascade of primary-response transcriptional regulators and secondary effector genes during the third larval instar and prepupal periods of development. The early ecdysone-response Broad-Complex (BR-C) gene, a key...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9879
更新日期:2000-11-01 00:00:00
abstract::All metazoan genomes encode multiple RAS GTPase activating proteins (RasGAPs) that negatively regulate the conserved RAS/MAPK signaling pathway. In mammals, several RasGAPs exhibit tumor suppressor activity by preventing excess RAS signal transduction. We have identified gap-3 as the to date missing Caenorhabditiseleg...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.08.026
更新日期:2008-11-15 00:00:00
abstract::Primary mesenchyme cell migration in the sea urchin embryo is inhibited by sulfate deprivation and exposure to exogenous beta-D-xylosides, two treatments known to disrupt proteoglycan synthesis. We show that in the developing sea urchin, exogenous xyloside affects the synthesis by the primary mesenchyme cells of a ver...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90089-l
更新日期:1991-02-01 00:00:00
abstract::Chromatin insulators orchestrate gene transcription during embryo development and cell differentiation by stabilizing interactions between distant genomic sites. Mutations in genes encoding insulator proteins are generally lethal, making in vivo functional analyses of insulator proteins difficult. In Drosophila, howev...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.03.024
更新日期:2015-07-01 00:00:00
abstract::The canonical, beta-catenin-dependent Wnt pathway is a crucial player in the early events of Xenopus development. Dorsal axis formation and mesoderm patterning are accepted effects of this pathway, but the regulation of expression of genes involved in mesoderm specification is not. This conclusion is based largely on ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0786
更新日期:2002-10-01 00:00:00
abstract::The Drosophila brain is tracheated by the cerebral trachea, a branch of the first segmental trachea of the embryo. During larval stages the cerebral trachea splits into several main (primary) branches that grow around the neuropile, forming a perineuropilar tracheal plexus (PNP) at the neuropile surface. Five primary ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.09.022
更新日期:2007-02-01 00:00:00
abstract::The experimental manipulation of early embryologic events, resulting in the misexpression of the homeobox transcription factor pitx2, is associated with subsequent defects of laterality in a number of vertebrate systems. To clarify the role of one pitx2 isoform, pitx2c, in determining the left-right axis of amphibian ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00389-0
更新日期:2003-10-15 00:00:00
abstract::The Drosophila ovarian germline stem cells (GSCs) constantly experience self-renewal and differentiation, ensuring the female fertility throughout life. The balance between GSC self-renewal and differentiation is exquisitely regulated by the stem cell niche, the stem cells themselves and systemic factors. Increasing e...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.04.015
更新日期:2013-07-15 00:00:00
abstract::The location and distribution of neural crest-derived Schwann cells during development of the peripheral nerves of chick forelimbs were examined using chick-quail chimeras. Neural crest cells were labeled by transplantation of the dorsal part of the neural tube from a quail donor to a chick host at levels of the neura...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90014-8
更新日期:1992-03-01 00:00:00
abstract::Hedgehog (Hh) proteins are secreted molecules that play an essential role in development and tumorigenesis. In Drosophila cultured cells, phosphorylation of the kinesin-like Costal2 (Cos2) protein at Ser572 is triggered by the kinase fused (Fu) upon Hh pathway activation. Here, we validate the first phospho-antibody f...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.04.021
更新日期:2010-08-01 00:00:00
abstract::We have analyzed several aspects of the development of flies carrying mutations at the Glued locus. Optic lobe abnormalities in individuals heterozygous for the original Glued allele were previously shown to result from an action of this mutation in the retinula cells. We have estimated when the functioning of this ge...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90256-7
更新日期:1983-09-01 00:00:00
abstract::T-box family transcription factors play many roles in Metazoan development. Here we characterise Tbx6r, a unique Tbx6 paralogue isolated from the amphibian Xenopus. The evolution and developmental integration of this divergent T-box gene within the vertebrates reveals an unexpected level of plasticity within this cons...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.01.013
更新日期:2010-04-01 00:00:00
abstract::The changes in the intracellular pH (pHi) of sea urchin sperm associated with motility initiation and acrosome reaction were investigated using uptake of two different probes; 9-aminoacridine and methylamine, as a qualitative index. Sperm suspended in Na+-free sea water were immotile and able to concentrate these amin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90004-0
更新日期:1983-01-01 00:00:00
abstract::Vitelline coats (VCs) of Phallusia mammillata were isolated and purified following homogenization of live eggs in order to investigate the molecular basis of sperm-egg recognition. Clean VCs were partly solubilized by sonication in H2O and the soluble fraction (SFVC), derived from the outer surface of VCs, was used fo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90272-5
更新日期:1991-12-01 00:00:00
abstract::The relationship between cellular position and growth control has been studied in cultures of dissociated fragments of mouse limb bud cells. Using cells derived from various positions along the anterior-posterior axis of the limb bud we have developed culture conditions that optimize growth of positionally isolated ce...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90208-x
更新日期:1992-05-01 00:00:00
abstract::Eight series of mouse chimeras were produced by aggregating 8-cell embryos that differed at the Gpi-1s locus, encoding glucose phosphate isomerase (GPI-1). Chimeric blastocysts (Gpi-1sa/Gpi-1sa <--> Gpi-1sb/Gpi-1sb) were transferred to pseudopregnant females, which produced only GPI-1C enzyme. Quantitative electrophor...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1062
更新日期:1995-03-01 00:00:00
abstract::The Id1 and Id3 genes play major roles during cardiac development, despite their expression being confined to non-myocardial layers (endocardium-endothelium-epicardium). We previously described that Id1Id3 double knockout (dKO) mouse embryos die at mid-gestation from multiple cardiac defects, but early lethality precl...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.10.004
更新日期:2011-01-01 00:00:00
abstract::We have isolated Xbr-1, a novel Xenopus homeobox gene that defines a new class of homeobox genes, distantly related to the Drosophila BarH1 and BarH2 genes. Xbr-1 is predominantly expressed in the developing Xenopus eye, starting as early as the neural plate stage. At early stages of eye development XBR-1 is expressed...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0055
更新日期:1996-02-25 00:00:00
abstract::The rise of evolutionary developmental biology was not the progressive isolation and characterization of developmental genes and gene networks. Many obstacles had to be overcome: the idea that all genes were more or less involved in development; the evidence that developmental processes in insects had nothing in commo...
journal_title:Developmental biology
pub_type: 历史文章,杂志文章
doi:10.1016/j.ydbio.2011.03.013
更新日期:2011-09-01 00:00:00
abstract::Ca2+-sensitive microelectrodes were used to study changes in cytoplasmic free calcium during progesterone-induced meiotic maturation in Xenopus laevis oocytes. In contrast to previous reports, no changes were detected during the maturation process, although a large activation pulse of Ca2+ was seen when in vitro matur...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90475-0
更新日期:1985-06-01 00:00:00
abstract::Branching morphogenesis, the process by which cells or tissues generate tree-like networks that function to increase surface area or in contacting multiple targets, is a common developmental motif in multicellular organisms. We use Drosophila tracheal terminal cells, a component of the insect respiratory system, to in...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.02.021
更新日期:2014-06-01 00:00:00
abstract::Selected stages of the developing chicken cornea have been examined for type VI collagen, employing monoclonal antibodies specific for this molecule. By immunofluorescence, the molecule is not detectable in 5 1/2 day corneas, a time at which the epithelial-derived, acellular primary stroma is the only corneal matrix p...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90013-8
更新日期:1986-12-01 00:00:00