Abstract:
:We have analyzed several aspects of the development of flies carrying mutations at the Glued locus. Optic lobe abnormalities in individuals heterozygous for the original Glued allele were previously shown to result from an action of this mutation in the retinula cells. We have estimated when the functioning of this gene or its product is required for normal visual system development by using genetic mosaicism induced by somatic recombination and temperature shifts of a temperature-sensitive mutation at this locus. Both methods point to a period in the mid-third instar, suggesting that early events in the formation of ommatidia and/or late events in the program of retinal cells are affected. Application of a new histological stain for developing axons indicates that individuals heterozygous for Glued exhibit abnormalities in the retinula fiber projection by the late third instar. Thus, the adult phenotype is not solely the result of later cellular degeneration or rearrangement. Beneath M+ Gl+ clones which encompass the entire eye were found optic lobe abnormalities with features not seen in either other mosaics or Gl heterozygotes. The possibility that these abnormalities result from temporal asynchrony in the development of eye and and optic lobe in these individuals is discussed and the results of attempts to test this hypothesis are presented.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Harte PJ,Kankel DRdoi
10.1016/0012-1606(83)90256-7subject
Has Abstractpub_date
1983-09-01 00:00:00pages
88-102issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(83)90256-7journal_volume
99pub_type
杂志文章abstract::Horseradish peroxidase (HRP) was used as an intracellular lineage tracer in two experiments designed to reveal the sites of origin of cells that formed the duplicate embryo which developed in relation to an organizer grafted in the ventral marginal zone (VMZ) of Xenopus laevis embryos. In the first experiment a dorsal...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90180-5
更新日期:1984-03-01 00:00:00
abstract::In Drosophila, the RNA helicase VASA (VAS) is required for both germ line formation and oocyte differentiation. While the murine VAS homologue is required for spermatogenesis, it is dispensable for germ line formation. The molecular basis for this apparently dual role of VAS in germ line ontogeny is, however, unclear....
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.04.004
更新日期:2004-07-01 00:00:00
abstract::We report here the identification and characterization of a novel gene, T1 alpha, expressed in high abundance in adult rat lung, fetal lung, and early fetal brain. T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. The cDNA for...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1024
更新日期:1995-01-01 00:00:00
abstract::Tyrosinase is the rate-limiting enzyme for the production of melanin pigmentation. In the mouse and other animals, homozygous null mutations in the Tyrosinase gene (Tyr) result in the absence of pigmentation, i.e. albinism. Here we used the CRISPR/Cas9 system to generate mono- and bi-allelic null mutations in the Tyr ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.06.017
更新日期:2014-09-01 00:00:00
abstract::Tissue specificity of cell adhesion was directly characterized in a unit cell interaction using a novel laser trapping cell manipulator in combination with a fixed micropipet. We quantified the adhesive specificity of endodermal and ectodermal epithelial cells from Hydra, which are known to sort out within hours after...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1068
更新日期:1994-03-01 00:00:00
abstract::Botryllus schlosseri is a colonial marine urochordate in which all adult organisms (called zooids) in a colony die synchronously by apoptosis (programmed cell death) in cyclical fashion. During this death phase called takeover, cell corpses within the dying organism are engulfed by circulating phagocytic cells. The "o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0772
更新日期:2002-09-15 00:00:00
abstract::Murine ovarian folliculogenesis commences after birth involving oocyte growth, somatic cell differentiation and structural remodeling of follicle stromal boundaries. The extracellular metalloproteinase ADAMTS-1 has activity against proteoglycans and collagen and is produced by the granulosa cells of ovarian follicles....
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.10.012
更新日期:2006-12-15 00:00:00
abstract::It is known that secreted proteins from the anterior lateral endoderm, FGF8 and BMP2, are involved in mesodermal cardiac differentiation, which determines the first cardiac field, defined by the expression of the earliest specific cardiac markers Nkx-2.5 and Gata4. However, the molecular mechanisms responsible for ear...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.07.007
更新日期:2015-10-01 00:00:00
abstract::PDGFB is a growth factor which is vital for the completion of normal prenatal development. In this study, we report the phenotypic analysis of placentas from mouse conceptuses that lack a functional PDGFB or PDGFRbeta gene. Placentas of both types of mutant exhibit changes in the labyrinthine layer, including dilated ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9306
更新日期:1999-08-01 00:00:00
abstract::Extensive elongation of lens fiber cells is a central feature of lens morphogenesis. Our study investigates the role of N-cadherin junctions in this process in vivo. We investigate both the molecular players involved in N-cadherin junctional maturation and the subsequent function of these junctions as epicenters for t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.10.009
更新日期:2011-01-15 00:00:00
abstract::There has been a resurgence of interest in comparative embryology. It is now important to be able to compare gene expression in different species at similar developmental stages. One phenomenon which may make it difficult to compare embryos in this way is heterochrony--a change in developmental timing during evolution...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1006/dbio.1995.8041
更新日期:1995-12-01 00:00:00
abstract::The lens in the vertebrate eye has been shown to be critical for proper differentiation of the surrounding ocular tissues including the cornea, iris and ciliary body. In mice, previous investigators have assayed the consequences of molecular ablation of the lens. However, in these studies, lens ablation was initiated ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.08.016
更新日期:2008-11-01 00:00:00
abstract::We have identified cDNA clones encoding a testis-specific poly(A) polymerase, termed TPAP, a candidate molecule responsible for cytoplasmic polyadenylation of preexisting mRNAs in male haploid germ cells. The TPAP gene was most abundantly expressed coincident with the additional elongation of mRNA poly(A) tails in rou...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9894
更新日期:2000-12-01 00:00:00
abstract::Oral-facial-digital type I (OFDI) syndrome is an X-linked male lethal developmental disorder. It is ascribed to ciliary dysfunction and characterized by malformation of the face, oral cavity, and digits. Conditional inactivation using different Cre lines allowed us to study the role of the Ofd1 transcript in limb deve...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.09.020
更新日期:2011-01-15 00:00:00
abstract::Skeletal muscles of the avian limb are derived from mononucleated myogenic precursor cells (myoblasts) that migrate into the somatopleural mesoderm of the developing limb bud from the ventrolateral dermomyotome of limb adjacent somites. In the present study, we utilized replication-deficient lacZ-encoding retroviruses...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(02)00028-3
更新日期:2003-01-15 00:00:00
abstract::It has been suggested that the mesonephros has a role in normal limb development. This hypothesis was directly tested by removing the mesonephros adjacent to the presumptive limb region of stage 12-18 chick embryos using microsurgery or laser ablation. The experimental manipulation resulted in reduced limb outgrowth o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90230-e
更新日期:1992-05-01 00:00:00
abstract::We have cloned a Caenorhabditis elegans homologue of the Drosophila gap gene hunchback (hb) and have designated it hbl-1 (hunchback-like). hbl-1 encodes a predicted 982-amino-acid protein, containing two putative zinc-finger domains similar to those of Drosophila Hunchback. The gene is transcribed embryonically, but u...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9096
更新日期:1999-01-15 00:00:00
abstract::Hedgehog (Hh) proteins and cAMP-dependent protein kinase A (PKA) generally play opposing roles in developmental patterning events. Humans and mice heterozygous for mutations in the sonic hedgehog (Shh) receptor gene patched-1 (ptc1) have an increased incidence of certain types of cancer, including medulloblastoma (MB)...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.10.031
更新日期:2008-01-01 00:00:00
abstract::Tbx18 has been shown to be essential for ureteral development. However, it remains unclear whether it plays a direct role in kidney development. Here we addressed this by focusing on examining the pattern and contribution of Tbx18+ cells in the kidney and its role in kidney vascular development. Expression studies and...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.04.006
更新日期:2014-07-01 00:00:00
abstract::The adrenal cortex has a complex vasculature that is essential for growth, tissue maintenance, and access of secreted steroids to the bloodstream. However, the interaction between vasculature and adrenal cortex during early organogenesis remains largely unclear. In this study, we focused on the zebrafish counterpart o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.04.464
更新日期:2006-09-01 00:00:00
abstract::During endochondral bone formation, vascular invasion initiates the replacement of avascular cartilage by bone. We demonstrate herein that the cartilage-specific overexpression of VEGF-A(164) in mice results in the hypervascularization of soft connective tissues away from cartilage. Unexpectedly, perichondrial tissue ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.05.552
更新日期:2009-08-15 00:00:00
abstract::Cerebral and spinal vascular systems are organized individually, and they then conjugate at their border, through the integration of basilar artery and vertebral arteries. Zebrafish (Danio rerio) is an ideal organism for studying early vascular development, and the precise procedure of cranial and truncal vascular for...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.07.015
更新日期:2015-10-01 00:00:00
abstract::We present evidence to show that differentiation in wing cells to produce hairs is synchronous over the distal 90% of the wing surface (approximately 28,000 cells). In spite of this synchrony within such a large area a temporal gradient exists between zones (in general anterior to posterior) on the animal surface with...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90047-7
更新日期:1983-02-01 00:00:00
abstract::The rabbit sperm membrane autoantigen RSA-1 is a sialoglycoprotein of 13,000 daltons which first appears on the surface of pachytene spermatocytes. Using specific antiserum to RSA-1 the antigen has been localized by immunofluorescence and immunoperoxidase staining. On testicular cells labeled at 37 degrees C, RSA-1 is...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(81)90400-0
更新日期:1981-06-01 00:00:00
abstract::The surface of eggs of the ascidian Halocynthia roretzi, observed with SEM, is essentially smooth until immediately before cell division when numerous microvilli appear and remain during cytokinesis. As the dividing blastomeres become closely adherent, however, the microvilli disappear and the eggs recover their smoot...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90214-8
更新日期:1984-04-01 00:00:00
abstract::The eukaryotic initiation translation factor eIF6 is a highly conserved, essential protein implicated in translation. eIF6 is regulated in vivo by extracellular signals, such as IGF signaling (for a review see Miluzio et al., 2009). In Xenopus, eif6 over-expression causes a delay in eye development (De Marco et al., 2...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.04.017
更新日期:2017-07-01 00:00:00
abstract::This study evaluated Na/K-ATPase alpha 1- and alpha 3-subunit isoform polypeptide expression and localization during bovine preattachment development. Na/K-ATPase cation transport activity from the one-cell to blastocyst stage was also determined by measuring ouabain-sensitive 86Rb+ uptake. Both alpha1- and alpha 3-su...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8874
更新日期:1998-05-01 00:00:00
abstract::The current gene regulatory network (GRN) for the sea urchin embryo pertains to pregastrular specification functions in the endomesodermal territories. Here we extend gene regulatory network analysis to the adjacent oral and aboral ectoderm territories over the same period. A large fraction of the regulatory genes pre...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.02.029
更新日期:2009-05-15 00:00:00
abstract::The expression of the neural cell adhesion molecules N-CAM and L1 was investigated in the olfactory system of the mouse using immunocytochemical and immunochemical techniques. In the olfactory epithelium, globose basal cells and olfactory neurons were stained by the polyclonal N-CAM antibody reacting with all three co...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90397-1
更新日期:1988-10-01 00:00:00
abstract::During limb development, Sonic hedgehog (SHH) and HOX proteins are considered among the most important factors regulating digit number and identity. SHH signaling prevents the processing of GLI3 into a short form that functions as a strong transcriptional repressor. Gli3 mutant limbs are characterized by a severe poly...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.07.023
更新日期:2007-10-15 00:00:00