Abstract:
:Urodele amphibians can regenerate their limbs. During limb regeneration, dermal fibroblasts are transformed into undifferentiated cells called blastema cells. These dermis-blastema cells show multipotency. Such so-called endogenous reprogramming of cell differentiation is one of the main targets of amphibian limb regeneration studies. It is well recognized that nerve presence controls the initiation of limb regeneration. Accordingly, nerve factors have been sought in amphibian limb regeneration. To investigate it, a relatively new study system called the accessory limb model (ALM) was developed. Using ALM, two signaling cascades (Fgf and Gdf5 signaling) came under focus. In the present study, Growth and differentiation factor-5 (Gdf5) application to wounded skin initiated limb regeneration responses and resulted in induction of a blastema-like structure in the absence of a nerve. However, the Gdf5-induced structure showed defects as a regeneration blastema, such as absence of detectable Prrx1 expression by in situ hybridization. The defects could be remedied by additional Fibroblasts growth factor (Fgf) inputs. These two inputs (Gdf5 and Fgfs) were sufficient to substitute for the nerve functions in the induction of limb regeneration. Indeed, Fgf2, Fgf8, and Gdf5 applications with the contralateral skin graft resulted in limb formation without nerve supply. Furthermore, acquisition of cartilage differentiation potential of dermal fibroblasts was tested in an in vivo and in vitro combination assay. Dermal fibroblasts cultured with Gdf5 were difficult to participate in cartilage formation when the cultured cells were grafted into cartilage forming region. In contrast, dermal fibroblasts cultured with Fgf2 and Fgf8 became easier to participate into cartilage formation in the same procedure. These results contribute to our understanding of molecular mechanisms of the early phase of amphibian limb regeneration.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Makanae A,Hirata A,Honjo Y,Mitogawa K,Satoh Adoi
10.1016/j.ydbio.2013.05.010subject
Has Abstractpub_date
2013-09-01 00:00:00pages
213-26issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(13)00254-6journal_volume
381pub_type
杂志文章abstract::This study reports a morphological, skeletal, and molecular characterization of the supernumerary limbs induced by systemic administration of all-trans retinoic acid to egg-cylinder stage mouse embryos. As initially described by Rutledge et al. (Proc. Natl. Acad. Sci. USA 91, 5436, 1994), we have found that oral admin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0126
更新日期:1996-06-15 00:00:00
abstract::The relationship between ascorbic acid concentration and cellular transport mechanisms was studied in chicken embryos (Gallus gallus domesticus). Unincubated (Day 0) fertile eggs did not contain detectable levels of ascorbic acid as assayed by high performance liquid chromatography with electrochemical detection. Howe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90298-w
更新日期:1990-06-01 00:00:00
abstract::Immunological approaches were used to characterize an antigen that is present within the cortical granules of mouse oocytes and eggs. Immunoelectron microscopy shows a specific localization of the antigen to the cortical granules in the cortex of mouse oocytes and eggs. Following in vitro fertilization, the antigen is...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90392-v
更新日期:1990-10-01 00:00:00
abstract::External genital development begins with formation of paired genital swellings, which develop into the genital tubercle. Proximodistal outgrowth and axial patterning of the genital tubercle are coordinated to give rise to the penis or clitoris. The genital tubercle consists of lateral plate mesoderm, surface ectoderm,...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0668
更新日期:2002-07-01 00:00:00
abstract::John W. Saunders, Jr. made seminal discoveries unveiling how chick embryos develop their limbs. He discovered the apical ectodermal ridge (AER), the zone of polarizing activity (ZPA), and the domains of interdigital cell death within the developing limb and determined their function through experimental analysis. Thes...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2016.11.005
更新日期:2017-09-15 00:00:00
abstract::Integrin α5-null embryos die in mid-gestation from severe defects in cardiovascular morphogenesis, which stem from defective development of the neural crest, heart and vasculature. To investigate the role of integrin α5β1 in cardiovascular development, we used the Mesp1(Cre) knock-in strain of mice to ablate integrin ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.09.014
更新日期:2014-11-15 00:00:00
abstract::A complex regulatory cascade is required for normal cardiac development, and many aspects of this network are conserved from Drosophila to mammals. In Drosophila, the seven-up (svp) gene, an ortholog of the vertebrate chick ovalbumin upstream promoter transcription factors (COUP-TFI and II), is initially activated in ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.10.025
更新日期:2007-02-15 00:00:00
abstract::Using an in vitro model of rat epiphyseal chondrocyte differentiation in which cells are maintained in a three-dimensional cell pellet, we show that exogenous TGF-beta 1 reversibly prevents terminal differentiation of epiphyseal chondrocytes into hypertrophic cells. Through maintenance of gene expression for the carti...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1200
更新日期:1993-08-01 00:00:00
abstract::We have isolated Xbr-1, a novel Xenopus homeobox gene that defines a new class of homeobox genes, distantly related to the Drosophila BarH1 and BarH2 genes. Xbr-1 is predominantly expressed in the developing Xenopus eye, starting as early as the neural plate stage. At early stages of eye development XBR-1 is expressed...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0055
更新日期:1996-02-25 00:00:00
abstract::Heparan sulfate proteoglycans (HSPGs) regulate a number of major developmental processes, but their roles in synovial joint formation remain unknown. Here we created conditional mouse embryo mutants lacking Ext1 in developing joints by mating Ext1(f/f) and Gdf5-Cre mice. Ext1 encodes a subunit of the Ext1/Ext2 Golgi-a...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.12.022
更新日期:2011-03-01 00:00:00
abstract::The s-SHIP protein is a shorter isoform of the longer SHIP1 protein and lacks the N-terminal SH2 domain region contained in SHIP1. s-SHIP is expressed in ES cells and in enriched bone marrow stem cells, and may be controlled by a promoter within intron 5 of the ship1 gene. We therefore examined the potential specifici...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.04.032
更新日期:2005-07-15 00:00:00
abstract::All metazoan genomes encode multiple RAS GTPase activating proteins (RasGAPs) that negatively regulate the conserved RAS/MAPK signaling pathway. In mammals, several RasGAPs exhibit tumor suppressor activity by preventing excess RAS signal transduction. We have identified gap-3 as the to date missing Caenorhabditiseleg...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.08.026
更新日期:2008-11-15 00:00:00
abstract::Embryonic morphogenesis relies on the intrinsic ability of cells, often through remodeling the cytoskeleton, to shape epithelial tissues during development. Epithelial invagination is an example of morphogenesis that depends on this remodeling but the cellular mechanisms driving arrangement of cytoskeletal elements ne...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2020.02.014
更新日期:2020-06-01 00:00:00
abstract::The effect of phenylephrine, an alpha-1 adrenergic agonist, on development of body asymmetry was studied using a rat whole embryo culture system. Embryos were explanted at the presomite stage, cultured in 100% rat serum containing various concentrations of phenylephrine, and examined at the 20-25 somite stage for side...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90067-d
更新日期:1991-01-01 00:00:00
abstract::We previously isolated a p53 cDNA from a Xenopus oocyte library. To determine if p53 has a function in the developmental period, we have studied its expression at the RNA and protein levels during the early development of Xenopus laevis. Two p53 transcripts (3 and 2.2 kb) are expressed from the beginning of Xenopus oo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1230
更新日期:1993-09-01 00:00:00
abstract::The evolutionary divergence of cnidarian and bilaterian lineages from their remote metazoan ancestor occurred at an unknown depth in time before the Cambrian, since crown group representatives of each are found in Lower Cambrian fossil assemblages. We report here a variety of putative embryonic, larval, and adult micr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0714
更新日期:2002-08-01 00:00:00
abstract::Neurons and glia are produced in stereotyped patterns after neuroblast cell division during development of the Drosophila central nervous system. The first cell division of thoracic neuroblast 6-4 (NB6-4T) is asymmetric, giving rise to a glial precursor cell and a neuronal precursor cell. In contrast, abdominal NB6-4 ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9727
更新日期:2000-06-15 00:00:00
abstract::Drosophila nemo (nmo) and other Nemo-like kinase family members (Nlks) are well-established key regulators of numerous conserved signaling pathways, such as Wg and BMP. nmo mutants display pleiotropic defects at different developmental stages, including the embryo. In this study we describe a detailed characterization...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.04.008
更新日期:2010-07-01 00:00:00
abstract::Drosophila melanogaster mature oocytes in ovaries are arrested at metaphase I of meiosis. Eggs that have reached the uterus have released this arrest. It was not known where in the female reproductive tract egg activation occurs and what triggers it. We investigated when and where the egg is activated in Drosophila in...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0246
更新日期:2001-06-15 00:00:00
abstract::In the ascidian Ciona intestinalis, oral siphon amputation activates adult stem cell niches in the branchial sac to divide and dispatch migratory progenitor cells to a regeneration blastema at the site of injury. This study shows that progenitor cells derived from branchial sac stem cell niches have roles in homeostas...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.09.005
更新日期:2019-04-15 00:00:00
abstract::The vertebrate liver and heart arise from adjacent cell layers in the anterior lateral (AL) endoderm and mesoderm of late gastrula embryos, and the earliest stages of liver and heart development are interrelated through reciprocal tissue interactions. Although classical embryological studies performed several decades ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.01.019
更新日期:2004-04-15 00:00:00
abstract::Recently, it has been suggested that a mouse sperm-associated protein with properties similar to the inhibitory guanine nucleotide-binding regulatory protein Gi plays an intermediary role in the zona pellucida-induced acrosome reaction (Endo, Y., Lee, M. A., and Kopf, G. S. 1987. Dev. Biol. 119, 210-216). In the prese...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90157-1
更新日期:1988-09-01 00:00:00
abstract::A mouse mammary epithelial cell line with morphogenetic properties in vivo, Comma-Dbeta, was used to isolate and to characterize mammary progenitor cells. We found that a homogeneous cell population expressing high surface levels of stem cell antigen 1 (Sca-1) was able to give rise in vivo to ductal and alveolar struc...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.02.007
更新日期:2006-05-15 00:00:00
abstract::The scalloped and vestigial genes are both required for the formation of the Drosophila wing, and recent studies have indicated that they can function as a heterodimeric complex to regulate the expression of downstream target genes. We have analyzed the consequences of complete loss of scalloped function, ectopic expr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9939
更新日期:2000-12-15 00:00:00
abstract::Carbohydrate chains on the t12 mutant embryos were analyzed. No abnormalities of the synthesis of the carbohydrate chains were observed in the t12 homozygotes until the late morula stage, when radiolabeled carbohydrate chains were analyzed by Sephadex G-50 column chromatography. Furthermore, polarization of the Con A ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90309-0
更新日期:1988-08-01 00:00:00
abstract::The adrenal cortex has a complex vasculature that is essential for growth, tissue maintenance, and access of secreted steroids to the bloodstream. However, the interaction between vasculature and adrenal cortex during early organogenesis remains largely unclear. In this study, we focused on the zebrafish counterpart o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.04.464
更新日期:2006-09-01 00:00:00
abstract::To form a gonad, germ cells (GCs) and somatic gonadal precursor cells (SGPs) must migrate to the correct location in the developing embryo and establish the cell-cell interactions necessary to create proper gonad architecture. During gonad morphogenesis, SGPs send out cellular extensions to ensheath the individual GCs...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.04.027
更新日期:2012-07-15 00:00:00
abstract::The human kidneys filter 180 l of blood every day via about 2.5 million glomeruli. The three layers of the glomerular filtration apparatus consist of fenestrated endothelium, specialized extracellular matrix known as the glomerular basement membrane (GBM) and the podocyte foot processes with their modified adherens ju...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.10.047
更新日期:2008-01-15 00:00:00
abstract::A reexamination of the cDNA clones of the Strongylocentrotus purpuratus sea urchin egg receptor for sperm resulted in several important changes to the sequence. By using both rapid amplification of cDNA ends (RACE) and Northern blot analysis for confirmation, the corrected deduced amino acid sequence was shown to lack...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8504
更新日期:1997-04-01 00:00:00
abstract::The effect of hydrocortisone on the development of dorsal skin was analyzed in the chick embryo by (1) transmission electron microscopy, (2) indirect immunofluorescence histology of extracellular matrix components (collagen types I, III, and IV; fibronectin; and laminin), and (3) quantitative determination of collagen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90056-3
更新日期:1984-11-01 00:00:00