Abstract:
:The s-SHIP protein is a shorter isoform of the longer SHIP1 protein and lacks the N-terminal SH2 domain region contained in SHIP1. s-SHIP is expressed in ES cells and in enriched bone marrow stem cells, and may be controlled by a promoter within intron 5 of the ship1 gene. We therefore examined the potential specificity of promoter activity in ES cells of an intron 5/intron 6 ship1 genomic segment and its tissue specificity within transgenic mice expressing GFP from this promoter region. The results indicate that s-SHIP promoter activity is specific for ES cells in vitro and for known and presumptive stem/progenitor cells throughout embryo development of the transgenic mice. Specific GFP expression was observed in the blastocyst, primordial germ cells, thymus, arterioles, osteoblasts, and skin epidermis. The epidermis/epithelium is the progenitor for hair follicles, mammary tissue, and prostate. Interestingly, each of these latter tissues acquired a few GFP-positive cells in the course of their development from the epithelial layers, and these cells express marker proteins for stem/progenitor cells. These results identify potential stem cell populations, mark these cells for analyses in normal and cancer development, and implicate s-SHIP as an important protein in stem/progenitor cell function.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Rohrschneider LR,Custodio JM,Anderson TA,Miller CP,Gu Hdoi
10.1016/j.ydbio.2005.04.032subject
Has Abstractpub_date
2005-07-15 00:00:00pages
503-21issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(05)00270-8journal_volume
283pub_type
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journal_title:Developmental biology
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.10.025
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.07.010
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1226
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journal_title:Developmental biology
pub_type: 杂志文章
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