Abstract:
:We examined the effects of ciliary neurotrophic factor (CNTF) and depolarization, two environmental signals that influence noradrenergic and cholinergic function, on neuropeptide expression by cultured sympathetic neurons. Sciatic nerve extract, a rich source of CNTF, increased levels of vasoactive intestinal peptide (VIP), substance P, and somatostatin severalfold while significantly reducing levels of neuropeptide Y (NPY). No change was observed in the levels of leu-enkephalin (L-Enk). These effects were abolished by immunoprecipitation of CNTF-like molecules from the extract with an antiserum raised against recombinant CNTF, and recombinant CNTF caused changes in neuropeptide levels similar to those of sciatic nerve extract. Alterations in neuropeptide levels by CNTF were dose-dependent, with maximal induction at concentrations of 5-25 ng/ml. Peptide levels were altered after only 3 days of CNTF exposure and continued to change for 14 days. Depolarization of sympathetic neuron cultures with elevated potassium elicited a different spectrum of effects; it increased VIP and NPY content but did not alter substance P, somatostatin, or L-Enk. Depolarization is known to block cholinergic induction in response to heart cell conditioned medium and we found that it blocked the induction of choline acetyltransferase (ChAT) and peptides by recombinant cholinergic differentiation factor/leukemia inhibitory factor (CDF/LIF). In contrast, it did not antagonize the effects of CNTF on either ChAT activity or neuropeptide expression. Thus, while CNTF has effects on neurotransmitter properties similar to those previously reported for CDF/LIF, the actions of these two factors are differentially modulated by depolarization, suggesting that the mechanisms of cholinergic and neuropeptide induction for the two factors differ. In addition, in contrast to CDF/LIF, CNTF did not alter levels of ChAT, VIP, substance P, or somatostatin in cultured dorsal root ganglion neurons. These observations indicate that CNTF and depolarization affect the expression of neuropeptides by sympathetic neurons and provide evidence for an overlapping yet distinct spectrum of actions of the two neuronal differentiation factors, CNTF and CDF/LIF.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Rao MS,Tyrrell S,Landis SC,Patterson PHdoi
10.1016/0012-1606(92)90242-9subject
Has Abstractpub_date
1992-04-01 00:00:00pages
281-93issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(92)90242-9journal_volume
150pub_type
杂志文章abstract::Amphibian embryos from the genus Xenopus are among the best species for understanding early vertebrate development and for studying basic cell biological processes. Xenopus, and in particular the diploid Xenopus tropicalis, is also ideal for functional genomics. Understanding the behavior of genes in this accessible m...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.04.023
更新日期:2004-07-15 00:00:00
abstract::Development of the Drosophila embryonic midgut is dependent on a number of genes, including tinman and bagpipe, which are required for formation of the visceral mesoderm, and the homeotic genes and their targets, which act locally in the visceral mesoderm to direct formation of specific midgut constrictions. Here we r...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1171
更新日期:1995-06-01 00:00:00
abstract::Temporally-regulated maternal RNA translation is essential for embryonic development, with defective degradation resulting in stalled 2-cell embryos. We show that DDX1, a DEAD box protein implicated in RNA transport, may be a key regulator of maternal RNA utilization. DDX1 protein localizes exclusively to cytoplasmic ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.07.014
更新日期:2019-11-15 00:00:00
abstract::The uterus undergoes a series of dramatic changes in response to an implanting conceptus that, in some mammalian species, includes differentiation of the endometrial stroma into decidual tissue. This process, called decidualization, can be induced artificially in rodents indicating that the conceptus may not be essent...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.03.006
更新日期:2006-06-15 00:00:00
abstract::Alkaline phosphatase (AP) activity is expressed by endodermal cells of ascidian larvae. It was reported previously that the expression of AP activity is resistant to treatment with actinomycin D, a transcription inhibitor that inhibits the appearance of several other tissue-specific molecules and morphological markers...
journal_title:Developmental biology
pub_type: 杂志文章
doi:
更新日期:1998-06-15 00:00:00
abstract::Chromatin regulators contribute to the developmental control of gene expression. In the nematode Caenorhabditis elegans, the roles of chromatin regulation in development have been explored in several contexts, including vulval differentiation. The synthetic multivulva (synMuv) genes are regulators of vulval developmen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.05.018
更新日期:2014-08-15 00:00:00
abstract::Mutations at the Drosophila melanogaster locus female sterile (1) homeotic (fs(1)h) result in segmental abnormalities including missing organs and homeotic transformations in the progeny of mutant mothers. Homeotic transformations are enhanced when the zygotes carry one of several third chromosome mutations, specifica...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90392-1
更新日期:1986-03-01 00:00:00
abstract::During the process of gastrulation in the sea urchin embryo, the vegetal plate invaginates to form the archenteron, a long narrow tube that extends across the blastocoel. Rearrangement of cells within the archenteron is thought to be a key component of the process of archenteron elongation. While these cell rearrangem...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1154
更新日期:1994-06-01 00:00:00
abstract::Alveologenesis is the final step of lung maturation, which subdivides the alveolar region of the lung into smaller units called alveoli. Each of the nascent dividers serves as a new gas-exchange surface, and collectively they drastically increase the surface area for breathing. Disruption of alveologenesis results in ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.11.017
更新日期:2016-01-15 00:00:00
abstract::Metathoracic limb buds were removed unilaterally from Locusta migratoria embryos at 30% of embryonic development, thereby depriving limb-innervating neurons of the opportunity of innervating their normal target muscles. The operated embryos were allowed to hatch and develop to adulthood, and then the connections betwe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90335-5
更新日期:1985-02-01 00:00:00
abstract::The mab-21 gene was first identified because of its requirement for ray identity specification in Caenorhabditis elegans. It is now known to constitute a family of genes that are highly conserved from vertebrates to invertebrates, and two homologues Mab21l1 and Mab21l2 have been identified in many species. Here we des...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.07.016
更新日期:2004-10-15 00:00:00
abstract::The Drosophila brain is tracheated by the cerebral trachea, a branch of the first segmental trachea of the embryo. During larval stages the cerebral trachea splits into several main (primary) branches that grow around the neuropile, forming a perineuropilar tracheal plexus (PNP) at the neuropile surface. Five primary ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.09.022
更新日期:2007-02-01 00:00:00
abstract::Chromatin insulators orchestrate gene transcription during embryo development and cell differentiation by stabilizing interactions between distant genomic sites. Mutations in genes encoding insulator proteins are generally lethal, making in vivo functional analyses of insulator proteins difficult. In Drosophila, howev...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.03.024
更新日期:2015-07-01 00:00:00
abstract::Chromatin structure plays an important role in the regulation of gene expression. Methylation of lysine residues on histone tails is an epigenetic mark that influences chromatin repression when specifically imparted on lysines 9 and 27 of histone H3, and on lysine 20 of H4. Histone lysines can be mono-, di-, and trime...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.02.013
更新日期:2006-05-15 00:00:00
abstract::Immunological approaches were used to characterize an antigen that is present within the cortical granules of mouse oocytes and eggs. Immunoelectron microscopy shows a specific localization of the antigen to the cortical granules in the cortex of mouse oocytes and eggs. Following in vitro fertilization, the antigen is...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90392-v
更新日期:1990-10-01 00:00:00
abstract::Suppression of wing fate and specification of haltere fate in Drosophila by the homeotic gene Ultrabithorax is a classical example of Hox regulation of serial homology (Lewis, E.B. 1978. Nature 276, 565-570) and has served as a paradigm for understanding homeotic gene function. We have used DNA microarray analyses to ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.12.022
更新日期:2006-03-15 00:00:00
abstract::Growth and Differentiation Factor 1 (GDF-1) has been implicated in left-right patterning of the mouse embryo but has no other known function. Here, we demonstrate a genetic interaction between Gdf1 and Nodal during anterior axis development. Gdf1-/-;Nodal+/- mutants displayed several abnormalities that were not presen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.02.002
更新日期:2006-05-15 00:00:00
abstract::Fertilization overcomes meiotic arrest by triggering a series of biochemical events, resulting in activation of the egg. A small group of protein tyrosine kinases (PTKs) have been identified in eggs of invertebrates and lower vertebrates and inhibitor studies have suggested that they play a role in late events of egg ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8816
更新日期:1998-02-01 00:00:00
abstract::Acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF) are present in high levels in most areas of the embryonic rodent brain. To begin to understand the role of these growth factors in brain development, the effects of aFGF and bFGF on dissociated cell cultures prepared from embryonic and ne...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90143-5
更新日期:1992-08-01 00:00:00
abstract::Acquisition of limb-forming ability by discrete regions of the lateral plate of the chick embryo is thought to depend on a signaling cascade moving sequentially from the area of Hensen's node to the somitic mesoderm, the intermediate mesoderm, and then to the prospective limb-forming regions of the lateral plate (Step...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8646
更新日期:1997-08-15 00:00:00
abstract::The interstitial cells of hydra form a multipotent stem cell system, producing terminally differentiated nerve cells and nematocytes during asexual growth. Under well-fed conditions the interstitial cell population doubles in size every 4 days. We have investigated the possible role of nerve cells in regulating this b...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90089-2
更新日期:1985-08-01 00:00:00
abstract::The CRISPR/Cas9 system is a rapid, simple, and often extremely efficient gene editing method. This method has been used in a variety of organisms and cell types over the past several years. However, using this technology for generating gene-edited animals involves a number of obstacles. One such obstacle is mosaicism,...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2018.10.008
更新日期:2019-01-15 00:00:00
abstract::It has been suggested that the mesonephros has a role in normal limb development. This hypothesis was directly tested by removing the mesonephros adjacent to the presumptive limb region of stage 12-18 chick embryos using microsurgery or laser ablation. The experimental manipulation resulted in reduced limb outgrowth o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90230-e
更新日期:1992-05-01 00:00:00
abstract::Tissue specificity of cell adhesion was directly characterized in a unit cell interaction using a novel laser trapping cell manipulator in combination with a fixed micropipet. We quantified the adhesive specificity of endodermal and ectodermal epithelial cells from Hydra, which are known to sort out within hours after...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1068
更新日期:1994-03-01 00:00:00
abstract::An increase in the incidence of aneuploidy is well documented with increasing maternal age, in particular in human females. Remarkably, little is known regarding the underlying molecular basis for the age-associated increase in aneuploidy, which is a major source of decreased fertility in humans. Using mouse as a mode...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.01.048
更新日期:2008-04-15 00:00:00
abstract::Mutations impairing the GTPase activity of G protein Galpha subunits can result in activated Galpha subunits that affect signal transduction and cellular responses and, in some cases, promote tumor formation. An analogous mutation in the Dictyostelium Galpha4 subunit gene (Q200L substitution) was constructed and found...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9474
更新日期:1999-11-15 00:00:00
abstract::Pioneer axons in insect legs are experimentally accessible model systems for the molecular identification and cellular localization of guidance cues regulating the path of axon growth. A detailed study of the Fe2 pioneer axons in the legs of the cockroach was performed to examine the diversity of guidance mechanisms. ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8704
更新日期:1997-10-15 00:00:00
abstract::Syndecan, a cell surface proteoglycan, binds multiple extracellular ligands, and is developmentally regulated in epithelial and mesenchymal tissues. The branching morphogenesis of embryonic lung is dependent on epithelial-mesenchymal interactions and, based on studies with inhibitors, on proteoglycan synthesis. To ass...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90286-c
更新日期:1991-10-01 00:00:00
abstract::Sequence analysis of cDNA clones encoding fibronectin (FN) from Xenopus laevis reveals extensive amino acid identities with other vertebrate FNs, including the presence of the Arg-Gly-Asp (RGD) cell attachment site in type III-10 and of a second, cell-binding site (EILDV) in the alternative spliced V region of the pro...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90291-n
更新日期:1992-02-01 00:00:00
abstract::The aim of this study was to evaluate the role segmentation plays in the determination of neuronal identity in the hindbrain. We focused on two specific sets of hindbrain neurons, namely, the vestibulospinal and vestibulo-ocular neurons, which comprise distinct groups that can be identified and distinguished by virtue...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8986
更新日期:1998-10-01 00:00:00