Abstract：
:Mutations impairing the GTPase activity of G protein Galpha subunits can result in activated Galpha subunits that affect signal transduction and cellular responses and, in some cases, promote tumor formation. An analogous mutation in the Dictyostelium Galpha4 subunit gene (Q200L substitution) was constructed and found to inhibit Galpha4-mediated responses to folic acid, including the accumulation of cyclic nucleotides and chemotactic cell movement. The Galpha4-Q200L subunit also severely inhibited responses to cAMP, including cyclic nucleotide accumulation, cAMP chemotaxis, and cellular aggregation. An analogous mutation in the Galpha2 subunit (Q208L substitution), previously reported to inhibit cAMP responses (K. Okaichi et al., 1992, Mol. Biol. Cell 3, 735-747), was also found to partially inhibit folic acid chemotaxis. Chemotactic responses to folic acid and cAMP and developmental aggregation were also inhibited by a mutant Galpha5 subunit with the analogous alteration (Q199L substitution). All aggregation-defective Galpha mutants were capable of multicellular development after a temporary incubation at 4 degrees C and this development was found to be dependent on wild-type Galpha4 function. This study indicates that mutant Galpha subunits can inhibit signal transduction pathways mediated by other Galpha subunits.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Srinivasan J,Gundersen RE,Hadwiger JAdoi
10.1006/dbio.1999.9474subject
Has Abstractpub_date
1999-11-15 00:00:00pages
443-52issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(99)99474-5journal_volume
215pub_type
杂志文章abstract：:Drosophila thoracic muscles are comprised of both direct flight muscles (DFMs) and indirect flight muscles (IFMs). The IFMs can be further subdivided into dorsolongitudinal muscles (DLMs) and dorsoventral muscles (DVMs). The correct patterning of each category of muscles requires the coordination of specific executive...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00255-0
更新日期：2003-08-15 00:00:00
abstract：:Oligodendrocyte precursor cells (OPCs) can be differentiated in culture into either oligodendrocytes or type-2 astrocytes (2As), depending on the culture conditions. Whereas oligodendrocyte development can occur in the absence of inducing signals, 2A development apparently cannot. Fetal calf serum (FCS) and bone morph...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.11.013
更新日期：2004-03-01 00:00:00
abstract：:During metamorphosis, the steroid hormone 20-hydroxyecdysone induces morphogenesis of imaginal discs, including the formation of appendages. IMP-E2 is an ecdysone-dependent, single-copy Drosophila gene, whose transcripts accumulate rapidly in imaginal discs in response to the hormone. The IMP-E2 product is secreted at...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90084-v
更新日期：1990-08-01 00:00:00
abstract：:Bicaudal-D (Bic-D) is required for the transport of determinant mRNAs and proteins to the presumptive oocyte, an essential step in the differentiation of the oocyte. Bic-D protein contains four well-defined heptad repeat domains characteristic of intermediate filament proteins. We characterized the ovarian phenotypes ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0170
更新日期：2001-04-01 00:00:00
abstract：:N-cadherin is a Ca2+-dependent cell-cell adhesion molecule, which was identified in brain cells of mouse and chicken. In the present study, we have determined the pattern of expression of N-cadherin in chicken embryos at various stages by means of immunohistochemistry. N-cadherin was expressed in cells derived from al...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90119-9
更新日期：1987-03-01 00:00:00
abstract：:The mesoderm of the sea urchin embryo conventionally is divided into two populations of cells; the primary mesenchyme cells (PMCs), which produce the larval skeleton, and the secondary mesenchyme cells (SMCs), which differentiate into a variety of cell types but do not participate in skeletogenesis. In this study we e...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90220-5
更新日期：1988-02-01 00:00:00
abstract：:Immature, Stage VI oocytes of Xenopus laevis fail to activate (i.e., to propagate a cortical reaction and elevate a fertilization envelope) when pricked or exposed to A23187. We determined the times during maturation when immature oocytes treated with progesterone in vitro developed the capacity to respond to pricking...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90177-5
更新日期：1984-03-01 00:00:00
abstract：:The eukaryotic initiation translation factor eIF6 is a highly conserved, essential protein implicated in translation. eIF6 is regulated in vivo by extracellular signals, such as IGF signaling (for a review see Miluzio et al., 2009). In Xenopus, eif6 over-expression causes a delay in eye development (De Marco et al., 2...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.04.017
更新日期：2017-07-01 00:00:00
abstract：:Midline defects account for approximately 5% of congenital abnormalities observed at birth. However, the molecular mechanisms underlying the formation of the ventral body wall are not well understood. Recent studies linked mutations in Porcupine-an O-acetyl transferase mediating Wnt ligand acylation-with defects in th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.02.014
更新日期：2015-05-15 00:00:00
abstract：:Islet1 (Isl1) is a LIM homedomain protein that plays a pivotal role in cardiac progenitors of the second heart field. Here, lineage studies with an inducible isl1-cre demonstrated that most Isl1 progenitors have migrated into the heart by E9. Although Isl1 expression is downregulated in most cardiac progenitors as the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.12.048
更新日期：2007-04-01 00:00:00
abstract：:Stable subdivision of Drosophila limbs into Anterior (A) and Posterior (P) compartments is a consequence of asymmetric signaling by Hedgehog (Hh) from P to A cells. The activity of the homeodomain protein Engrailed (En) in P cells has been reported to help to generate this asymmetry by inducing the expression of hedge...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.01.006
更新日期：2009-03-15 00:00:00
abstract：:In many non-mammalian vertebrates, adult dentitions result from cyclical rounds of tooth regeneration wherein simple unicuspid teeth are replaced by more complex forms. Therefore and by contrast to mammalian models, the numerical majority of vertebrate teeth develop shape during the process of replacement. Here, we ex...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.02.007
更新日期：2013-05-15 00:00:00
abstract：:FbxA is a novel member of a family of proteins that contain an F-box and WD40 repeats and that target specific proteins for degradation via proteasomes. In fruiting bodies formed from cells where the fbxA gene is disrupted (fbxA(-) cells), the spore mass fails to fully ascend the stalk. In addition, fbxA(-) slugs cont...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9793
更新日期：2000-08-01 00:00:00
abstract：:In a previous study (K.-I. Sato et al., 1999, Dev. Biol. 209, 308-320), we presented evidence that a Src-related protein-tyrosine kinase (PTK), named Xyk, may act upstream of the calcium release in fertilization of the Xenopus egg. In the present study, we examined whether PTK activation of phospholipase Cgamma (PLCga...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9782
更新日期：2000-08-15 00:00:00
abstract：:We investigated the influence of the growth surface on the direction of Xenopus spinal neurite growth in the presence of a dc electric field of physiological magnitude. The direction of galvanotropism was determined by the substratum; neurites grew toward the negative electrode (cathode) on untreated Falcon tissue cul...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9039
更新日期：1998-11-15 00:00:00
abstract：:Projection neurons of the developing cerebral cortex are generated in the cerebral ventricular zone and subsequently move to the developing cortical plate via radial migration. Conversely, most inhibitory interneurons originate in the ganglionic eminences and enter the developing cortical plate by tangential migration...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.06.040
更新日期：2006-10-01 00:00:00
abstract：:The myogenic basic helix-loop-helix transcription factor myogenin plays an essential role in the differentiation of skeletal muscle and, secondarily, in rib and sternum formation during mouse development. However, virtually nothing is known about the quantitative requirements for myogenin in these processes. Here, we ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9182
更新日期：1999-04-01 00:00:00
abstract：:The formation of the spinal cord during early embryonic development in vertebrate embryos is a continuous process that begins at gastrulation and continues through to the completion of somitogenesis. Despite the conserved usage of patterning mechanisms and gene regulatory networks that act to generate specific spinal ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2017.01.021
更新日期：2017-12-01 00:00:00
abstract：:Tissue specificity of cell adhesion was directly characterized in a unit cell interaction using a novel laser trapping cell manipulator in combination with a fixed micropipet. We quantified the adhesive specificity of endodermal and ectodermal epithelial cells from Hydra, which are known to sort out within hours after...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1068
更新日期：1994-03-01 00:00:00
abstract：:Epigenetic gene control is involved in mechanisms of development. Little is known about the cooperation of nuclear and chromatin events in programmed differentiation from mouse embryonic stem cells (ESC). To address this, Oct3/4-positive ESC and differentiated progenies, Sox1-positive neural precursor cells (NPC) and ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.04.450
更新日期：2006-10-15 00:00:00
abstract：:CD9 is a membrane protein belonging to the tetraspanin family. Despite CD9's broad tissue distribution, the only abnormality observed in CD9-deficient mice was infertility of females, which was responsible for a defect in the sperm-egg fusion process. However, the function of CD9 in sperm-egg fusion is not clear at al...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0694
更新日期：2002-07-15 00:00:00
abstract：:We have previously reported the detection of cAMP waves within monolayers of aggregating Dictyostelium discoideum cells (K. J. Tomchik and P.N. Devreotes, 1981, Science 212, 443-446). The computer-assisted analysis presented here of the fluorographic images of the cAMP waves reveals (1) all the waves have a consistent...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90178-1
更新日期：1983-04-01 00:00:00
abstract：:Tube formation is a widespread process during organogenesis. Specific cellular behaviors participate in the invagination of epithelial monolayers that form tubes. However, little is known about the evolutionary mechanisms of cell assembly into tubes during development. In Caenorhabditis elegans, the detailed step-to-s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.10.029
更新日期：2004-02-15 00:00:00
abstract：:The lizards are evolutionarily the closest vertebrates to humans that demonstrate the ability to regenerate entire appendages containing cartilage, muscle, skin, and nervous tissue. We previously isolated PAX7-positive cells from muscle of the green anole lizard, Anolis carolinensis, that can differentiate into multin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.08.037
更新日期：2018-01-15 00:00:00
abstract：:A series of chimeras was generated by injecting single normal, parthenogenetic, or androgenetic blastomeres carrying transgenic markers under the zona pellucida of nontransgenic eight-cell embryos. These chimeras were cultured to the blastocyst stage and sectioned, and the transgenic component was detected by in situ ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(89)80028-4
更新日期：1989-02-01 00:00:00
abstract：:Skeletal muscle fibers express members of the myosin heavy chain (MyHC) gene family in a fiber-type-specific manner. In avian skeletal muscle it is the expression of the slow MyHC isoforms that most clearly distinguishes slow- from fast-contracting fiber types. Two hypotheses have been proposed to explain fiber-type-s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8619
更新日期：1997-08-01 00:00:00
abstract：:We have identified zebrafish orthologues of glial cell line-derived neurotrophic factor (GDNF) and the ligand-binding component of its receptor GFRalpha1. We examined the mRNA expression pattern of these genes in the developing spinal cord primary motor neurons (PMN), kidney, and enteric nervous systems (ENS) and have...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.0145
更新日期：2001-03-15 00:00:00
abstract：:Embryonic 4- to 15-day-old quail ciliary ganglia (CG) were grafted into the neural crest migration pathway of 2-day-old chick embryos at the adrenomedullary level of the neural axis. This back-transplantation results in dispersion of cells of the implanted ganglion, their migration in the host embryo, and subsequent p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90286-0
更新日期：1984-10-01 00:00:00
abstract：:We have used in situ hybridization and RNA blotting analysis to compare the timing of accumulation of poly(A) and alpha-subtype histone mRNA during oogenesis in the sea urchin Strongylocentrotus purpuratus. In situ hybridization with 3H-poly(U) shows that the content of poly(A) in the developing oocyte increases four-...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90161-1
更新日期：1984-02-01 00:00:00
abstract：:The outgrowth of motor axons to the developing pectoral fin of the Japanese medaka fish (Oryzias latipes) was investigated both in wildtype embryos and in the pectoral finless (pl) mutants in which adults are missing pectoral fins. Late in embryogenesis the pectoral fin is a simple limb which contains two antagonist m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90445-9
更新日期：1991-07-01 00:00:00
