Abstract:
:Oligodendrocyte precursor cells (OPCs) can be differentiated in culture into either oligodendrocytes or type-2 astrocytes (2As), depending on the culture conditions. Whereas oligodendrocyte development can occur in the absence of inducing signals, 2A development apparently cannot. Fetal calf serum (FCS) and bone morphogenetic proteins (BMPs) are powerful inducers of 2A development in culture, but there is no compelling evidence that OPCs develop into astrocytes in vivo. We show here that BMPs are made by glial cells in the developing rat optic nerve, raising the question of why 2As do not normally develop in the optic nerve. We demonstrate that the BMP antagonist Noggin is strongly expressed by both OPCs and type-1 astrocytes in the developing optic nerve. We also show that depletion of Noggin by a small interference RNA inhibits OPC proliferation and induces 2A differentiation in the presence of a low, non-2A-inducing concentration of FCS. By contrast, enforced expression of Noggin in OPCs blocks FCS-induced 2A differentiation. These findings suggest that BMPs in FCS are largely responsible for the 2A-inducing activity of FCS and that Noggin may normally inhibit the formation of 2As in the developing CNS.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Kondo T,Raff MCdoi
10.1016/j.ydbio.2003.11.013subject
Has Abstractpub_date
2004-03-01 00:00:00pages
242-51issue
1eissn
0012-1606issn
1095-564Xpii
S0012160603007231journal_volume
267pub_type
杂志文章abstract::Primitive blood constitutes the ventralmost mesoderm in amphibians, and its cleavage-stage origin reveals important clues about the orientation of the dorsal/ventral axis in the embryo. In recent years, investigators employing various lineage-labeling strategies have reported disparate results for the origin of primit...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0717
更新日期:2002-08-01 00:00:00
abstract::Zygotic Wnt signaling has been shown to be involved in dorsoventral mesodermal patterning in Xenopus embryos, but how it regulates different myogenic gene expression in the lateral mesodermal domains is not clear. Here, we use transient exposure of embryos or explants to lithium, which mimics Wnt/beta-catenin signalin...
journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2003-12-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1125
更新日期:1994-05-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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abstract::The developmental events of sporulation and fruiting body formation in the prokaryote Myxococcus xanthus are preceded by a stage of massive cell death. Two phenotypically complementable strains of M. xanthus defective in developmental lysis were identified from a group of conditional sporulation mutants. Mixture of th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90133-2
更新日期:1985-11-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
abstract::The evolution of canalized traits is a central question in evolutionary biology. Natural variation in highly conserved traits can provide clues about their evolutionary potential. Here we investigate natural variation in a conserved trait-even-skipped (eve) expression at the cellular blastoderm stage of embryonic deve...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.06.019
更新日期:2015-09-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.12.024
更新日期:2016-07-15 00:00:00
abstract:FOREWORD:The neural crest has been the main object of my investigations during my career in science, up to now. It is a fascinating topic for an embryologist because of its two unique characteristics: its large degree of multipotency and the fact that its development involves a phase during which its component cells mi...
journal_title:Developmental biology
pub_type: 杂志文章,评审
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abstract::The Ci-Dll-B gene is an early regulator of ectodermal development in the ascidian Ciona intestinalis (Imai et al., 2006). Ci-Dll-B is located in a convergently transcribed bigene cluster with a tandem duplicate, Ci-Dll-A. This clustered genomic arrangement is the same as those of the homologous vertebrate Dlx genes, w...
journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2011-05-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9246
更新日期:1999-05-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.10.029
更新日期:2004-02-15 00:00:00
abstract::Ca2+-sensitive microelectrodes were used to study changes in cytoplasmic free calcium during progesterone-induced meiotic maturation in Xenopus laevis oocytes. In contrast to previous reports, no changes were detected during the maturation process, although a large activation pulse of Ca2+ was seen when in vitro matur...
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pub_type: 杂志文章
doi:10.1016/0012-1606(85)90475-0
更新日期:1985-06-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1292
更新日期:1993-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-07-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0708
更新日期:2002-09-01 00:00:00
abstract::We have isolated a cDNA of the DEAD protein family from a Xenopus ovary cDNA library. Northern hybridization revealed that the mRNA corresponding to the cDNA [XVLG1 (Xenopus vasa-like gene)] was specifically expressed in adult testis and ovary. The deduced amino acid sequence of XVLG1 was relatively homologous to that...
journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1212
更新日期:1995-08-01 00:00:00