Abstract:
:In many organisms early embryogenesis is characterised by a period refractory to transcription. In Caenorhabditis elegans, the one-cell embryo is transcriptionally inactive, but at around eight-cell stage transcription is activated in the somatic lineage. This model suggests that histone tail modifications associated with activation of transcription, such as di- or trimethylation of histone 3 on lysine 4 (H3K4me2/me3) should be enriched in the somatic lineage. Here, we have investigated the deposition of H3K4me3 during embryogenesis and found that it is more dynamic than anticipated. In the eight-cell stage embryo, H3K4me3 deposition is poor in the germline blastomere, as expected, but surprisingly three somatic blastomeres also remain poor in H3K4me3. All the other somatic blastomeres show robust deposition of H3K4me3. Interestingly, the three somatic blastomeres poor in H3K4me3 are descendants of the first germline blastomere, implying an activity that impedes on H3K4me3 deposition in these cells. In contrast, the deposition of H3K4me2 and H3K27me2/3 is not lineage restricted. Taken together, our data reveal that H3K4me3 deposition is highly regulated according to the cell lineage involved.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Wang S,Fisher K,Poulin GBdoi
10.1016/j.ydbio.2011.04.010subject
Has Abstractpub_date
2011-07-15 00:00:00pages
227-38issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(11)00242-9journal_volume
355pub_type
杂志文章abstract::As the germ band shortens in Drosophila melanogaster embryos, cell shape changes cause segments to narrow anteroposteriorly and to lengthen dorsoventrally. One of the genes required for this retraction process is the hindsight (hnt) gene. hnt encodes a nuclear Zinc-finger protein that is expressed in the extraembryoni...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9409
更新日期:1999-10-01 00:00:00
abstract::In ascidian embryos, the earliest transcription from the zygotic genome begins between the 8-cell and 16-cell stages. Gata.a, a maternally expressed Gata transcription factor, activates target genes specifically in the animal hemisphere, whereas the complex of β-catenin and Tcf7 antagonizes the activity of Gata.a and ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.11.009
更新日期:2020-02-15 00:00:00
abstract::The role of retinoic acid (RA) in limb development is unclear, although it has been suggested to be a proximalizing factor which plays a morphogenetic role in pattern formation. Exogenous RA produces a teratogenic effect on limb morphology; similarly, changes in the endogenous distribution of RA following genetic abla...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.12.024
更新日期:2010-03-01 00:00:00
abstract::Accumulating evidence indicates that the endometrial extracellular matrix (ECM) modulates trophoblast adhesion during mouse blastocyst implantation. In previous studies of adhesion-competent mouse blastocysts, we have demonstrated that integrin-mediated fibronectin (FN)-binding activity on the apical surface of tropho...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0644
更新日期:2002-05-15 00:00:00
abstract::A number of genes encoding developmentally regulated mRNAs in the cellular slime mold, Dictyostelium discoideum, have been described. Many of these are regulated by cAMP. Analysis of the earliest time at which elevated levels of cAMP can induce the expression of these mRNAs reveals a more complex pattern of regulation...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90323-4
更新日期:1986-10-01 00:00:00
abstract::Cell polarity is thought to be required for the efficient production of nascent blastocoele fluid, which begins at the 16-cell stage of mouse preimplantation development. In this study the 4-cell/16-cell blastomere heterokaryon was used to test the hypothesis that solute transport across the apical membrane domain ind...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90041-9
更新日期:1989-06-01 00:00:00
abstract::The Hedgehog (Hh) signal transduction pathway plays a central role in the development of invertebrates and vertebrates. While much is known about the pathway, the role of Suppressor of fused (Su(fu)), a component of the pathway's signaling complex has remained enigmatic. Previous studies have linked Su(fu) to the cyto...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.02.050
更新日期:2006-06-01 00:00:00
abstract::The murine cpk mouse develops a rapid-onset polycystic kidney disease (PKD) with many similarities to human PKD. During kidney development, the transcription factor Pax2 is required for the specification and differentiation of the renal epithelium. In humans, Pax2 is also expressed in juvenile cystic kidneys where it ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9618
更新日期:2000-03-15 00:00:00
abstract::A simple assay has been developed to measure cGMP-specific phosphodiesterase (cGPD) activity in crude soluble extracts of amoebae of Dictyostelium discoideum. When amoebae of different wild-type strains were starved on buffered agar, all strains exhibited an 8- to 12-fold increase in cGMP-specific hydrolyzing activity...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90026-5
更新日期:1984-05-01 00:00:00
abstract::The 3' penultimate exon (exon 18) of transcripts from the muscle myosin heavy chain (MHC) gene of Drosophila melanogaster is excluded from mRNAs of embryonic and larval muscle, while it is included in mRNAs of adult thoracic muscles. By transforming organisms with the MHC gene 5' end, linked in-frame to the MHC gene 3...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90235-u
更新日期:1991-08-01 00:00:00
abstract::The GRAM domain was found in glucosyltransferases, myotubularins and other membrane-associated proteins. So far, functions for majority of these proteins are yet to be uncovered. In order to address the evolutionary and functional significance of this family members, we have performed a comprehensive investigation on ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.11.031
更新日期:2008-02-15 00:00:00
abstract::Hippo (Hpo) signaling plays a critical role in restricting tissue growth and organ size in both invertebrate and vertebrate animals. However, how the Hpo kinase is regulated during development has not been clearly understood. Using a Bimolecular Fluorescence Complementation assay, we have investigated the functional s...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.12.017
更新日期:2013-03-15 00:00:00
abstract::In the absence of maternal expression of the pecanex gene, the embryo develops severe hyperneuralization similar to that characteristic of Notch mutant embryos. We have extended a previous molecular analysis of the chromosomal interval that encompasses pecanex by using additional deficiencies to localize the locus on ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90127-9
更新日期:1989-11-01 00:00:00
abstract::Neuronal circuit development and function require proper synapse formation and maintenance. Genetic screens are one powerful method to identify the mechanisms shaping synaptic development and stability. However, genes with essential roles in non-neural tissues may be missed in traditional loss-of-function screens. In ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.04.008
更新日期:2012-06-15 00:00:00
abstract::Lineage diversification in the vertebrate neural crest may occur via instructive signals acting on pluripotent cells, and/or via early specification of subpopulations towards particular lineages. Mesencephalic neural crest cells normally form cholinergic parasympathetic neurons in the ciliary ganglion, while trunk neu...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.10.024
更新日期:2005-02-01 00:00:00
abstract::The nuclear lamina is an extensive protein network that underlies the inner nuclear envelope. This network includes the LAP2-emerin-MAN1-domain (LEM-D) protein family, proteins that share an association with the chromatin binding protein Barrier-to-autointegration factor (BAF). Loss of individual LEM-D proteins causes...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.05.001
更新日期:2016-07-01 00:00:00
abstract::Urodele amphibians can regenerate their limbs. During limb regeneration, dermal fibroblasts are transformed into undifferentiated cells called blastema cells. These dermis-blastema cells show multipotency. Such so-called endogenous reprogramming of cell differentiation is one of the main targets of amphibian limb rege...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.05.010
更新日期:2013-09-01 00:00:00
abstract::At gastrulation in the sea urchin embryo dramatic cell adhesion changes contribute to primary mesenchyme cell ingression movements and to cell rearrangements during archenteron invagination. At ingression, quantitative adhesion assays demonstrated previously that primary mesenchyme cells (PMCs) change their affinity f...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9165
更新日期:1999-03-01 00:00:00
abstract::The serine/threonine kinase LKB1 is a master kinase that regulates a number of critical events such as cell transformation, polarization, development, stress response, and energy metabolism in metazoa. After multiple unsuccessful attempts of generating Dictyostelium lkb1-null cells, an RNAi-based knockdown approach pr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.10.006
更新日期:2011-12-15 00:00:00
abstract::The development of germ cells is a highly ordered process that begins during fetal growth and is completed in the adult. Epigenetic modifications that occur in germ cells are important for germ cell function and for post-fertilization embryonic development. We have previously shown that male germ cells in the adult mo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.05.002
更新日期:2007-07-15 00:00:00
abstract::From July 19-24, 2015, 169 clinicians and basic scientists gathered in the vertiginous heights of Snowmass, Colorado (2502 m) for the fourth FASEB summer research conference on the 'Biology of Cilia and Flagella'. Organizers Maureen Barr (Rutgers University), Iain Drummond (Massachusetts General Hospital/Harvard Medic...
journal_title:Developmental biology
pub_type:
doi:10.1016/j.ydbio.2015.10.030
更新日期:2016-01-15 00:00:00
abstract::Using gene-specific probes derived from four mouse gamma-crystallin cDNAs, we have examined the regulation of different members of the mouse gamma-crystallin gene family during lens development. Our analysis revealed that, while the different gamma-crystallin genes appear to be coordinately activated during embryogene...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90227-2
更新日期:1987-01-01 00:00:00
abstract::The prostate gland develops from the urogenital sinus by a testosterone-dependent process of ductal morphogenesis. Sonic hedgehog (Shh) is expressed in the urogenital sinus epithelium and the time course of expression coincides with the formation of the main prostatic ducts. Expression is most abundant in the lumen of...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9229
更新日期:1999-05-01 00:00:00
abstract::We recently demonstrated a critical role for two-pore channel type 2 (TPC2)-mediated Ca2+ release during the differentiation of slow (skeletal) muscle cells (SMC) in intact zebrafish embryos, via the introduction of a translational-blocking morpholino antisense oligonucleotide (MO). Here, we extend our study and demon...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.03.031
更新日期:2017-05-15 00:00:00
abstract::Zebrafish heart regeneration relies on the capacity of cardiomyocytes to proliferate upon injury. To understand the principles of this process after cryoinjury-induced myocardial infarction, we established a spatio-temporal map of mitotic cardiomyocytes and their differentiation dynamics. Immunodetection of phosphohis...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.12.002
更新日期:2015-03-01 00:00:00
abstract::A cDNA library was used to measure changes in many individual mRNAs during muscle differentiation in culture. A library of 1000 clones was constructed from total myofiber poly(A) RNA. About 23% of these clones gave a detectable colony hybridization signal using end-labeled myofiber mRNA, the remainder containing muscl...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90049-0
更新日期:1983-02-01 00:00:00
abstract::Vulval epithelial tubes invaginate through concerted cell migration, ring formation, stacking of rings and intra-ring cell fusion in the nematodes Caenorhabditis elegans, Oscheius tipulae and Pristionchus pacificus. The number of rings forming the invaginations is invariantly seven, six, and eight, respectively. We hy...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.10.010
更新日期:2008-01-01 00:00:00
abstract::Cardiomyocyte differentiation overall has been analyzed in vivo and in vitro at the molecular level by homologous recombination, gene mutation studies, and by transgenics; however, the roles of many signal transduction mechanisms that drive this differentiation process are still not fully understood. One set of signal...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(02)00080-5
更新日期:2003-03-15 00:00:00
abstract::The spermatid nucleus and cytoplasm undergo dramatic morphological modifications during spermatid differentiation into mature sperm. Some of the external force causing this nuclear shaping is generated by a microtubular structure termed the manchette, which attaches to the perinuclear ring of the spermatid. Here, we r...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0286
更新日期:2001-08-15 00:00:00
abstract::Drosophila thoracic muscles are comprised of both direct flight muscles (DFMs) and indirect flight muscles (IFMs). The IFMs can be further subdivided into dorsolongitudinal muscles (DLMs) and dorsoventral muscles (DVMs). The correct patterning of each category of muscles requires the coordination of specific executive...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00255-0
更新日期:2003-08-15 00:00:00