Abstract：
:A cDNA library was used to measure changes in many individual mRNAs during muscle differentiation in culture. A library of 1000 clones was constructed from total myofiber poly(A) RNA. About 23% of these clones gave a detectable colony hybridization signal using end-labeled myofiber mRNA, the remainder containing muscle sequences too rare to be detected with this assay. The 230 positive clones were grouped into four classes based on relative visual intensity. Reconstruction experiments using pure globin mRNA enable us to determine the approximate percentage of total RNA made up by each mRNA hybridizing to a cDNA clone. Those clones containing sequences complementary to developmentally regulated mRNAs were identified by a differential hybridization procedure. The cDNA library was screened with end-labeled mRNA from both undifferentiated myoblasts and differentiated myofibers. Although the bulk of the clones hybridized essentially the same with both RNA populations, several dozen were found which hybridized differentially. Some clones contained sequences which were not present at all in myoblasts and present in very high quantities in myofibers. Others contained sequences found in both myoblasts and myofibers but in increased quantities in the differentiated cells. Still others contained sequences which decreased in quantity during muscle differentiation. The clones in the first group were chosen for immediate analysis since they likely contain contractile protein mRNA sequences. However, all the characterized cDNA clones can now be used as probes to study the chromosomal organization and developmental expression of genes active during muscle differentiation.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Devlin RB,Haskell SGdoi
10.1016/0012-1606(83)90049-0subject
Has Abstractpub_date
1983-02-01 00:00:00pages
476-83issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(83)90049-0journal_volume
95pub_type
杂志文章abstract：:Botryllus schlosseri is a colonial marine urochordate in which all adult organisms (called zooids) in a colony die synchronously by apoptosis (programmed cell death) in cyclical fashion. During this death phase called takeover, cell corpses within the dying organism are engulfed by circulating phagocytic cells. The "o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0772
更新日期：2002-09-15 00:00:00
abstract：:CD9 is a membrane protein belonging to the tetraspanin family. Despite CD9's broad tissue distribution, the only abnormality observed in CD9-deficient mice was infertility of females, which was responsible for a defect in the sperm-egg fusion process. However, the function of CD9 in sperm-egg fusion is not clear at al...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0694
更新日期：2002-07-15 00:00:00
abstract：:Hox genes are highly conserved segmental identity genes well known for their complex expression patterns and divergent targets. Here we present an analysis of cis-regulatory elements in the Caenorhabditis elegans Hox gene egl-5, which is expressed in multiple tissues in the posterior region of the nematode. We have ut...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.09.012
更新日期：2004-12-15 00:00:00
abstract：:Bone Morphogenetic Proteins (BMPs) have multiple activities in the developing spinal cord: they specify the identity of the dorsal-most neuronal populations and then direct the trajectories of dorsal interneuron (dI) 1 commissural axons. How are these activities decoded by dorsal neurons to result in different cellula...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.05.014
更新日期：2012-07-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90090-6
更新日期：1990-08-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.04.010
更新日期：2015-06-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90276-8
更新日期：1984-09-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.09.018
更新日期：2010-12-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.12.020
更新日期：2021-01-09 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.07.010
更新日期：2003-11-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1024
更新日期：1993-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0341
更新日期：1996-12-15 00:00:00
abstract：:In the embryonic mouse retina, retinoic acid (RA) is unevenly distributed along the dorsoventral axis: RA-rich zones in dorsal and ventral retina are separated by a horizontal RA-poor stripe that contains the RA-inactivating enzyme CYP26A1. To explore the developmental role of this arrangement, we studied formation of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.08.032
更新日期：2004-12-01 00:00:00
abstract：:Regulated changes in the cell cycle underlie many aspects of growth and differentiation. Prior to meiosis, germ cell cycles in many organisms become accelerated, synchronized, and modified to lack cytokinesis. These changes cause cysts of interconnected germ cells to form that typically contain 2(n) cells. In Drosophi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9570
更新日期：2000-02-01 00:00:00
abstract：:The axonal functions that act in the formation of the neuronal network have been shown to occur in close interdependence with the tissue that surrounds the growing axons. However, little is known about the molecular building blocks underlying axonal functions, although more than 400 axonal proteins have been identifie...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90090-4
更新日期：1986-11-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/0012-1606(85)90461-0
更新日期：1985-06-01 00:00:00
abstract：:A reexamination of the cDNA clones of the Strongylocentrotus purpuratus sea urchin egg receptor for sperm resulted in several important changes to the sequence. By using both rapid amplification of cDNA ends (RACE) and Northern blot analysis for confirmation, the corrected deduced amino acid sequence was shown to lack...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8504
更新日期：1997-04-01 00:00:00
abstract：:Previous studies have established that embryonal carcinoma (EC) cells produce several different growth factors, but express few, if any, receptors for epidermal growth factor, platelet-derived growth factor, or transforming growth factor type-beta. In this study, the production and utilization of fibroblast growth fac...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90161-3
更新日期：1988-09-01 00:00:00
abstract：:The GRAM domain was found in glucosyltransferases, myotubularins and other membrane-associated proteins. So far, functions for majority of these proteins are yet to be uncovered. In order to address the evolutionary and functional significance of this family members, we have performed a comprehensive investigation on ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.11.031
更新日期：2008-02-15 00:00:00
abstract：:The contributions that control of cell proliferation and cell growth make to developmental regulation of organ and body size remain poorly explored, particularly with respect to endocycles in polyploid tissues. The epithelium of the marine chordate Oikopleura dioica is composed of a fixed number of cells grouped in te...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0834
更新日期：2002-12-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.01.003
更新日期：2006-04-15 00:00:00
abstract：:We have performed a detailed analysis of the expression pattern of the three gnathostome Otx classes in order to gain new insights into their functional evolution. Expression patterns were examined in the developing eye of a chondrichthyan, the dogfish, and an amniote, the chick, and compared with the capacity of para...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.11.019
更新日期：2005-02-15 00:00:00
abstract：:Individual somatic muscles and heart progenitors are specified at defined positions within the mesodermal layer of Drosophila. The expression of the homeobox gene even-skipped (eve) identifies one specific subset of cells in the dorsal mesoderm, which give rise to particular pericardial cells and dorsal body wall musc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0397
更新日期：2001-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9066
更新日期：1998-12-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.04.038
更新日期：2009-07-15 00:00:00
abstract：:Xenopus laevis is among the few species that are capable of fully regenerating a lost lens de novo. This occurs upon removal of the lens, when secreted factors from the retina are permitted to reach the cornea epithelium and trigger it to form a new lens. Although many studies have investigated the retinal factors tha...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.12.036
更新日期：2014-02-15 00:00:00
abstract：:teashirt (tsh) encodes a zinc-finger protein that is thought to be part of a network that contributes to regionalization of the Drosophila embryo and establishes the domains of Hox protein function. tsh and the Hox gene Sex combs reduced (Scr) are essential to establish the identity of the first thoracic segment. We u...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.04.028
更新日期：2007-07-01 00:00:00
abstract：:One rapid response of starfish oocytes to the maturation-inducing hormone, 1-methyladenine (1-MA), is the formation of transient actin-filled spikes on the cell surface. The presence and distribution of G- and F-actin and several actin-associated proteins were examined in cortices isolated from oocytes before, during,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90140-4
更新日期：1984-02-01 00:00:00
abstract：:Metamorphosis is a postembryonic developmental process that involves morphophysiological and behavioral changes, allowing organisms to adapt into a novel environment. In some amphibians, aquatic organisms undergo metamorphosis to adapt in a terrestrial environment. In this process, these organisms experience major cha...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.08.002
更新日期：2020-10-01 00:00:00
abstract：:The arterial pole of the heart is the region where the ventricular myocardium continues as the vascular smooth muscle tunics of the aorta and pulmonary trunk. It has been shown that the arterial pole myocardium derives from the secondary heart field and the smooth muscle tunic of the aorta and pulmonary trunk derives ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.02.012
更新日期：2005-05-01 00:00:00
