Abstract:
:Artificial extracellular matrices composed of collagen, glycosaminoglycans (GAG), proteoglycans (PG), plasma fibronectin (FN), and a hyaluronate-binding protein (HABP) have been prepared that morphologically resemble embryonic extracellular matrices in vivo at the light and electron microscope level. The effect of each of the above matrix molecules on the structure and "self-assembly" of these artificial matrices was delineated. (1) Matrix components assembled in vitro morphologically resemble their counterparts in vivo, for the most part. Scanning and transmission electron microscopy indicate that under our assembly and fixation conditions, collagen forms striated fibrils that are 125 nm in diameter, FN forms 30- to 60-nm granules, chondroitin sulfate proteoglycan (CSPG) forms 27- to 37-nm granules, chondroitin sulfate (CS) assembles into 100- to 250-nm spheres, and hyaluronate (HA) appears either as granular mats when fixed with cetylpyridinium chloride (CPC) or as 1.5- to 3-nm microfibrils when preserved with ruthenium red plus tannic acid. These molecules are known to assume the same configurations in embryonic matrices when the same preservation techniques are used with the exception of FN, which generally forms fibrillar arrays. (2) Addition of various matrix molecules can radically change the appearance of the collage gels. HA greatly expands the volume of the gel and increases the space between collagen fibrils. CSPG at low concentrations (less than 1 mg/ml) and CS at high concentrations (greater than 20 mg/ml) bundle the collagen fibrils into twisted ropes. (3) A variety of assays were used to examine binding between various matrix components and retention of these components in the hydrated collagen lattices. These assays included solid-phase binding assays, negative staining of spread mixtures of matrix components, cryostat sections of unfixed mixtures of matrix components, and retention of radiolabeled matrix molecules in fixed and washed gels. A number of these binding interactions may play a role in the assembly and stabilization of the matrix. (a) HA, CSPG, and FN bind to collagen. CS appears to only weakly bind to collagen, if at all. (b) FN promotes the increased retention of HA, CSPG, and to a very small degrees, CS, in collagen gels. Conversely, the GAG increase the retention of 3H-FN in the gels. Furthermore, FN binds to HA, CS, and CSPG as demonstrated by solid surface binding assays and morphological criteria. The increased retention of GAG and CSPG by the addition of FN may be due to both stabilization of binding to the collagen and trapping of matrix complexes within the gel. (c) HA binds to both CS and CSPG.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Turley EA,Erickson CA,Tucker RPdoi
10.1016/0012-1606(85)90461-0subject
Has Abstractpub_date
1985-06-01 00:00:00pages
347-69issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(85)90461-0journal_volume
109pub_type
杂志文章,评审abstract::This first study of the onset of spermatogenesis in the sea urchin, Strongylocentrotus purpuratus, was undertaken using individuals reared in the laboratory. Spermatogenesis commences about 11-12 months after metamorphosis in these animals. Bindin message accumulates in late spermatocytes and early spermatids which li...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90149-d
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9878
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90235-1
更新日期:1987-04-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90256-x
更新日期:1989-12-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.07.003
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.02.007
更新日期:2017-04-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:1999-05-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00250-1
更新日期:2003-09-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90028-4
更新日期:1985-03-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.05.024
更新日期:2012-08-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.12.018
更新日期:2005-03-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.12.015
更新日期:2006-05-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.04.008
更新日期:2012-06-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.03.044
更新日期:2008-07-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90063-5
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
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pub_type: 杂志文章,评审
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:1990-10-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2001-01-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2017-11-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00