Abstract:
:The hox genes play a central role in organogenesis and are implicated in the formation of hematopoietic stem cells (HSCs). The cdx genes encode homeodomain transcription factors that act as master regulators of the hox genes. In zebrafish, mutations in cdx4 cause a severe, but not complete, deficit in embryonic blood cells. Here, we report the expression and function of cdx1a, a zebrafish Cdx1 paralogue. Using morpholino-mediated knockdown of cdx1a in a cdx4 mutant background, we show that a deficiency in both cdx genes causes a severe perturbation of hox gene expression and a complete failure to specify blood. The hematopoietic defect in cdx-deficient embryos does not result from a general block in posterior mesoderm differentiation as endothelial cells and kidney progenitors are still formed in the doubly deficient embryos. In addition, cdx-deficient embryos display a significant reduction in runx1a(+) putative HSCs in the zebrafish equivalent to the aorta-gonad-mesonephros (AGM) region. Overexpressing hoxa9a in cdx-deficient embryos rescues embryonic erythropoiesis in the posterior mesoderm as well as the formation of HSCs in the AGM region. Taken together, these results suggest that the cdx-hox pathway plays an essential role in the formation of both embryonic erythroid cells and definitive HSCs during vertebrate embryogenesis.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Davidson AJ,Zon LIdoi
10.1016/j.ydbio.2006.01.003subject
Has Abstractpub_date
2006-04-15 00:00:00pages
506-18issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(06)00009-1journal_volume
292pub_type
杂志文章abstract::The Hedgehog (Hh) signaling pathway is essential for patterning many structures in vertebrates including the nervous system, chordamesoderm, limb and endodermal organs. In the sea urchin, a basal deuterostome, Hh signaling is shown to participate in organizing the mesoderm. At gastrulation the Hh ligand is expressed b...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.04.018
更新日期:2009-07-01 00:00:00
abstract::Receptor protein tyrosine phosphatases (RPTPs) have been shown to play key roles in regulating axon guidance and synaptogenesis. HmLAR2, one of two closely related LAR-like RPTPs in the embryonic leech, is expressed in a few central neurons and in a unique segmentally-iterated peripheral cell, the comb cell (CC). Here...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.05.522
更新日期:2008-08-01 00:00:00
abstract::Coordinated locomotion of Drosophila larvae depends on accurate patterning and stable attachment to the cuticle of both muscles and proprioceptors (chordotonal organs). Unlike muscle spindles in mammals, the fly chordotonal organs are not embedded in the body-wall muscles. Yet, the contractile system (muscles and tend...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.11.022
更新日期:2010-01-15 00:00:00
abstract::Experiments in which the developing gut of avian embryos was back-transplanted to permit the bowel to interact with the developing neural tube were undertaken. Segments of intestine from 4-day quail embryos were implanted between the somites and neural tubes of chick embryos of 7 to 24 somites. The spinal cord respond...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90486-6
更新日期:1987-12-01 00:00:00
abstract::Cyclic protein-2 (CP-2) is secreted in vitro in substantial amounts by mature rat Sertoli cells in intact Stage VI and Stage VII seminiferous tubules. This stage-dependent secretion has led us to postulate that the biosynthesis of this molecule is stimulated by germ cells at a specific state of development. In order t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90412-5
更新日期:1988-11-01 00:00:00
abstract::Reproductive plasticity is the ability of an animal to modulate its reproductive functions in response to environmental changes. For example, Caenorhabditis elegans, a free-living nematode, can adjust the onsets of oogenesis and embryogenesis under harsh environmental conditions, including starvation. However, the mol...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.08.014
更新日期:2017-10-01 00:00:00
abstract::Early spermatids contain roughly 1000-fold more TATA-binding protein (TBP) mRNA than do somatic cells. The appearance of TBP-overexpressing spermatids in the developing testis is accompanied by a large increase in whole-organ levels of total RNA and of poly(A)+ RNA per cell. Whereas somatic cells initiate transcriptio...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8514
更新日期:1997-04-01 00:00:00
abstract::Members of the basic helix-loop-helix family of DNA binding proteins have important roles in the development of subpopulations of neural crest-derived neurons. We have cloned the chicken homologues of dHAND (HAND2) and eHAND (HAND1), basic helix-loop-helix DNA binding proteins whose neuronal expression is restricted t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9450
更新日期:1999-11-01 00:00:00
abstract::Growth factors and the extracellular matrix have been shown to fulfill vital developmental roles in many embryonic systems. Our hypothesis is that a developmental role played by the extracellular matrix in sea urchins may be the binding of a PDGF-like growth factor to promote signaling activity. We report here that an...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.8059
更新日期:1995-12-01 00:00:00
abstract::beta-Catenin was originally identified biochemically as a protein that binds E-cadherin in cultured cells and that interaction was later shown to be essential for cadherin function. Independently, armadillo, the beta-catenin homolog in Drosophila melanogaster, was identified as a segment polarity gene necessary for th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8739
更新日期:1997-12-15 00:00:00
abstract::In Drosophila, wing hairs are aligned in a distally oriented, parallel array. The frizzled pathway determines proximal-distal cell polarity in the wing; however, in frizzled pathway mutants, wing hairs remain parallel. How wing hairs align has not been determined. We have demonstrated a novel role for the septate junc...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.07.040
更新日期:2004-11-15 00:00:00
abstract::The dorsal air sacs supply oxygen to the flight muscles of the Drosophila adult. This tracheal organ grows from an epithelial tube (the air sac primordium (ASP)) that arises during the third larval instar (L3) from a wing-disc-associated tracheal branch. Since the ASP is generated by a program of both morphogenesis an...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.09.005
更新日期:2005-11-01 00:00:00
abstract::Drosophila Polycomb Repressive Complex 2 (PRC2) is a lysine methyltransferase that trimethylates histone H3 lysine 27 (H3K27me3), a modification essential for Polycomb silencing. Mutations in its catalytic subunit, E(Z), that abolish its methyltransferase activity disrupt Polycomb silencing, causing derepression of Po...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.12.007
更新日期:2012-04-15 00:00:00
abstract::A rapid, gentle technique is described for the isolation of nuclei from sea urchin embryos. Using this technique, we have analyzed the synthesis and accumulation of nonhistone nuclear proteins during sea urchin development by two-dimensional gel electrophoresis. Most nuclear proteins fall into one of three patterns of...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90445-3
更新日期:1987-09-01 00:00:00
abstract::Elastic fibers are produced during fetal and neonatal periods, and by maturity elastin expression has ceased. Fetal bovine hyaline chondrocytes acquired an elastogenic phenotype within 24 hr after isolation from the tissue, even though the tissue does not produce elastic fibers or tropoelastin mRNA in vivo. By multipl...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1140
更新日期:1994-05-01 00:00:00
abstract::The levels of al-1 and al-2 transcripts change dramatically in response to light and development during the formation of Neurospora crassa asexual spores (conidia). al-1 and al-2 mRNAs accumulate throughout conidiation irrespective of lighting conditions initially at low levels. As conidiation proceeds, two increases ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1129
更新日期:1995-05-01 00:00:00
abstract::The regulation of Sertoli cell transferrin and sulfated glycoprotein-2 (SGP-2) mRNA levels by vitamin A was studied in vitamin A deficient rats. Vitamin A deficiency differentially affected the levels of these mRNAs. Sertoli cells from vitamin A deficient rats contained 3-fold less transferrin mRNA and 1.8-fold more S...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90167-9
更新日期:1987-06-01 00:00:00
abstract::Epithelial planar cell polarity (PCP) allows epithelial cells to coordinate their development to that of the tissue in which they reside. The mechanisms that impart PCP as well as effectors that execute the polarizing instructions are being sought in many tissues. We report that the epidermal epithelium of Drosophila ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.04.454
更新日期:2006-09-15 00:00:00
abstract::Extensive elongation of lens fiber cells is a central feature of lens morphogenesis. Our study investigates the role of N-cadherin junctions in this process in vivo. We investigate both the molecular players involved in N-cadherin junctional maturation and the subsequent function of these junctions as epicenters for t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.10.009
更新日期:2011-01-15 00:00:00
abstract::Antenna structure varies widely among insects, in contrast to the well-conserved structure of legs. The adult capitate antenna of the red flour beetle, Tribolium castaneum, is composed of eleven articles, organized into four distinct morphological regions (scape, pedicel, funicle and club). Here, we report the use of ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.10.047
更新日期:2009-03-01 00:00:00
abstract::During development of the vertebrate visual system, retinal ganglion cell (RGC) axons follow a precise path toward their midbrain targets. Although much is known about the cues that direct RGC axons once they have left the optic disc, less is known about the guidance of axons at earlier stages, when RGCs first send ou...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9312
更新日期:1999-07-01 00:00:00
abstract::Notch activation inhibits neuronal differentiation during development of the nervous system; however, the dynamic role of Notch signaling in individual cell lineages remains poorly understood. We have characterized 3.4 kb 5'-regulatory sequence of a Notch target gene, her4, and used it to drive fluorescent gene expres...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.10.020
更新日期:2007-01-15 00:00:00
abstract::We report periodic movements during erection of Dictyostelium discoideum (Dd) sorocarps. Our observations lead to the working hypothesis that Dd sorocarp erection occurs by two superimposed processes: one periodic, with a modal period of 6 1/2 min, and one continuous. We tentatively identify the periodic process with ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(76)90237-2
更新日期:1976-09-01 00:00:00
abstract::The Drosophila segment polarity gene known as shaggy or zeste white 3 encodes a ubiquitously expressed serine-threonine protein kinase which is critical for a number of important developmental processes. In the developing wing blade, clones of cells lacking the normal shaggy-zeste white 3 product form dense tufts of m...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1081
更新日期:1994-03-01 00:00:00
abstract::We examined the effects of ciliary neurotrophic factor (CNTF) and depolarization, two environmental signals that influence noradrenergic and cholinergic function, on neuropeptide expression by cultured sympathetic neurons. Sciatic nerve extract, a rich source of CNTF, increased levels of vasoactive intestinal peptide ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90242-9
更新日期:1992-04-01 00:00:00
abstract::We have investigated the function of the neurogenic gene Notch (N) during development of the adult sensilla of Drosophila. Heat pulses were applied to flies carrying the temperature-sensitive Notch allele Nts1 at different larval and pupal stages. We can show that the reduction of Notch+ function during a short interv...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90147-b
更新日期:1990-11-01 00:00:00
abstract::Previous studies from this laboratory have identified a novel alpha-D-mannosidase on the sperm plasma membranes of several species, including man, which may have a role in fertilization. The polyclonal antibody raised against an isoform of the enzyme purified from rat epididymal fluid was found to cross-react with the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1050
更新日期:1995-02-01 00:00:00
abstract::Rapidly growing human teratocarcinoma cells (Tera-2) can be induced to differentiate into quiescent, nontumorigenic cells expressing neuronal markers. To more closely mimic the in vivo conditions for tumor growth, we grew Tera-2 cells in three-dimensional collagen gel cultures. The undifferentiated cells proliferated ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1016
更新日期:1994-01-01 00:00:00
abstract::Proper development of a multicellular organism relies on well-coordinated regulation of cell fate specification, cell proliferation and cell differentiation. The C. elegans postembryonic mesoderm provides a useful system for uncovering factors involved in these processes and for further dissecting their regulatory rel...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.06.011
更新日期:2017-09-01 00:00:00
abstract::Despite undergoing normal development and acquiring normal morphology and motility, mutations in spe-38 or trp-3/spe-41 cause identical phenotypes in Caenorhabditis elegans-mutant sperm fail to fertilize oocytes despite direct contact. SPE-38 is a novel, four-pass transmembrane protein and TRP-3/SPE-41 is a Ca(2+)-per...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.02.037
更新日期:2012-05-15 00:00:00