Abstract：
:Growth factors and the extracellular matrix have been shown to fulfill vital developmental roles in many embryonic systems. Our hypothesis is that a developmental role played by the extracellular matrix in sea urchins may be the binding of a PDGF-like growth factor to promote signaling activity. We report here that anti-human PDGF-B antibodies and anti-human TGF-alpha antibodies immunoprecipitated specific proteins isolated from Lytechinus embryos. Addition of these antibodies to Lytechinus embryos inhibited gastrulation and spiculogenesis. The embryos are sensitive to the antibodies from the four-cell through the hatching blastula stages, which suggests that the TGF-alpha-like and PDGF-like ligands are required for the early differentiation of the gut and spicules. We present evidence that the PDGF-like growth factor depends on the extracellular matrix for signaling activity. Synthetic peptides representing the heparan sulfate proteoglycan binding sequence on human PDGF-B were added to Lytechinus embryo cultures to compete for binding sites with the endogenous PDGF-like growth factor. The experimental peptide inhibited gastrulation and caused radially arranged multiple spicules to form. Development was unaffected by a control peptide. These studies support our hypothesis and suggest that TGF-alpha-like and PDGF-like growth factors induce signaling events required for sea urchin gastrulation and spiculogenesis and suggest that an extracellular matrix-associated PDGF-like growth factor is involved in differentiation along the oral-aboral axis.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Govindarajan V,Ramachandran RK,George JM,Shakes DC,Tomlinson CRdoi
10.1006/dbio.1995.8059subject
Has Abstractpub_date
1995-12-01 00:00:00pages
541-51issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(85)78059-1journal_volume
172pub_type
杂志文章abstract：:Vertebrate heart development requires specification of cardiac precursor cells, migration of cardiac progenitors as well as coordinated cell movements during looping and septation. DM-GRASP/ALCAM/CD166 is a member of the neuronal immunoglobulin domain superfamily of cell adhesion molecules and was recently suggested t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.06.013
更新日期：2008-09-01 00:00:00
abstract：:Inverse gradients of transcriptional repressors antagonize the transcriptional effector response to morphogens. However, the role of such inverse regulation might not manifest solely from lack of repressors. Sonic hedgehog (Shh) patterns the forebrain by being expressed ventrally; however, absence of antagonizing Gli3...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.03.011
更新日期：2019-06-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90106-x
更新日期：1985-08-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90142-9
更新日期：1986-08-01 00:00:00
abstract：:Retinal ganglion cells (RGCs) are the first cell type to differentiate during retinal histogenesis. It has been postulated that specified RGCs subsequently influence the number and fate of the remaining progenitors to produce the rest of the retinal cell types. However, several genetic knockout models have argued agai...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2008-04-15 00:00:00
abstract：:Tabby is a mouse mutant characterized by deficient development of the ectodermal organs: teeth, hair, and a subset of glands. Ectodysplasin, the protein encoded by the Tabby gene, was recently identified as a novel TNF-like transmembrane protein but little is known about its function. We have examined the Tabby tooth ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9514
更新日期：1999-12-15 00:00:00
abstract：:The spermatid nucleus and cytoplasm undergo dramatic morphological modifications during spermatid differentiation into mature sperm. Some of the external force causing this nuclear shaping is generated by a microtubular structure termed the manchette, which attaches to the perinuclear ring of the spermatid. Here, we r...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2001-08-15 00:00:00
abstract：:The segmental origin and migratory pattern of neural crest cells at the trunk level of avian embryos was studied, with special emphasis on the formation of the dorsal root ganglia (DRG) which organize in the anterior half of each somite. Neural crest cells were visualized using the quail-chick marker and HNK-1 immunof...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90236-3
更新日期：1987-04-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1275
更新日期：1995-09-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.12.026
更新日期：2016-02-15 00:00:00
abstract：:Alveologenesis is the final step of lung maturation, which subdivides the alveolar region of the lung into smaller units called alveoli. Each of the nascent dividers serves as a new gas-exchange surface, and collectively they drastically increase the surface area for breathing. Disruption of alveologenesis results in ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.11.017
更新日期：2016-01-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.03.020
更新日期：2018-06-15 00:00:00
abstract：:Previous studies have shown that cultured Schwann cells secrete platelet-derived growth factor (PDGF) and express PDGF receptors which transmit a mitogenic response particularly when their levels are upregulated by elevation of intracellular cAMP. In this study the expression of PDGF and PDGF alpha- and beta-receptors...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1044
更新日期：1993-02-01 00:00:00
abstract：:A chronic neuromuscular blockade during those embryonic stages when naturally occurring spinal motoneuron death occurs, results in the prevention of this cell loss. The excess motoneurons are maintained as long as the neuromuscular blockade is continued; once embryonic neuromuscular activity resumes, however, the exce...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90114-3
更新日期：1984-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90142-5
更新日期：1989-11-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00028-3
更新日期：2003-01-15 00:00:00
abstract：:We previously isolated a p53 cDNA from a Xenopus oocyte library. To determine if p53 has a function in the developmental period, we have studied its expression at the RNA and protein levels during the early development of Xenopus laevis. Two p53 transcripts (3 and 2.2 kb) are expressed from the beginning of Xenopus oo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1230
更新日期：1993-09-01 00:00:00
abstract：:Developmental effects of six mutations in the gene encoding the majority of alpha-tubulin in all tissues at all stages of Drosophila melanogaster development have been examined. All six alleles produce at least partially stable alpha 84B protein. In genetic assays, two of these alleles approximate the null condition. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90211-9
更新日期：1987-01-01 00:00:00
abstract：:Hair shafts are produced from stem cells located in the bulge. Our knowledge of the genetic pathways regulating cell fate acquisition in the immediate descendents of these stem cells, and fate maintenance in their committed progeny, is still incomplete. One pathway involved in fate maintenance within the hair matrix i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.11.021
更新日期：2009-02-15 00:00:00
abstract：:The neural crest is a transient and multipotent cell population arising at the edge of the neural plate in vertebrates. Recent findings highlight that neural crest patterning is initiated during gastrulation, i.e. earlier than classically described, in a progenitor domain named the neural border. This chapter reviews ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
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更新日期：2012-06-01 00:00:00
abstract：:A new monoclonal antibody, specific to an epitope in the carboxyl terminus of the Drosophila collagen IV molecule (basement membrane collagen) was identified. The distributions of collagen IV, laminin, and an additional extracellular molecule, the 2G2 antigen (2G2-Ag), were followed immunocytochemically during early w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1068
更新日期：1995-03-01 00:00:00
abstract：:Pax3 is a transcription factor expressed in somitic mesoderm, dorsal neural tube and pre-migratory neural crest during embryonic development. We have previously identified cis-acting enhancer elements within the proximal upstream genomic region of Pax3 that are sufficient to direct functional expression of Pax3 in neu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.12.030
更新日期：2010-03-15 00:00:00
abstract：:The development of asymmetric patterns along biologically relevant axes is a hallmark of many vertebrate organs or structures. One example is the sensory epithelium of the mammalian auditory system. Two distinct types of mechanosensory hair cells (inner and outer) and at least six types of associated supporting cells ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.06.003
更新日期：2019-09-15 00:00:00
abstract：:Exposure of Xenopus animal pole explants to transforming growth factor beta 2 (TGF-beta 2) induced the sequential expression of muscle nicotinic acetylcholine receptor (AChRs) isoforms and their corresponding mRNAs in cells which normally give rise to ectoderm. Single channel recordings revealed two functional classes...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1318
更新日期：1994-11-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0174
更新日期：1996-08-01 00:00:00
abstract：:The cardiac neural crest contains ectomesenchymal and neural anlagen that are necessary for normal heart development. It is not known whether other regions of the neural crest are capable of supporting normal heart development. In the experiments reported herein, quail donor embryos provided cardiac, trunk, or mesence...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90112-7
更新日期：1989-08-01 00:00:00
abstract：:In many organisms early embryogenesis is characterised by a period refractory to transcription. In Caenorhabditis elegans, the one-cell embryo is transcriptionally inactive, but at around eight-cell stage transcription is activated in the somatic lineage. This model suggests that histone tail modifications associated ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.04.010
更新日期：2011-07-15 00:00:00
abstract：:The Mesp bHLH genes play a conserved role during segmental patterning of the mesoderm in the vertebrate embryo by specifying segmental boundaries and anteroposterior (A-P) segmental polarity. Here we use a xenotransgenic approach to compare the transcriptional enhancers that drive expression of the Mesp genes within s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.12.038
更新日期：2008-03-15 00:00:00
abstract：:We have used amphibian gastrulation as a model system to study the action of the extracellular matrix (ECM) glycoprotein tenascin on mesodermal cell migration. Tenascin function was assayed in vitro during spreading of isolated cells from the dorsal marginal zone (DMZ) and during cell migration from DMZ explants. Plas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90256-i
更新日期：1990-02-01 00:00:00
abstract：:Despite undergoing normal development and acquiring normal morphology and motility, mutations in spe-38 or trp-3/spe-41 cause identical phenotypes in Caenorhabditis elegans-mutant sperm fail to fertilize oocytes despite direct contact. SPE-38 is a novel, four-pass transmembrane protein and TRP-3/SPE-41 is a Ca(2+)-per...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.02.037
更新日期：2012-05-15 00:00:00
