Abstract：
:Developmental effects of six mutations in the gene encoding the majority of alpha-tubulin in all tissues at all stages of Drosophila melanogaster development have been examined. All six alleles produce at least partially stable alpha 84B protein. In genetic assays, two of these alleles approximate the null condition. The other four alleles appear to form a graded series of hypomorphs. The two most severe alleles produce a semidominant maternal-effect polyphasic lethality, plus a predominantly larval recessive zygotic lethality. Clonal analysis of one of these alleles suggests it is a cell lethal. Worsening of the lethal phenotype (negative complementation) occurs in most interallelic heterozygotes involving these two mutations. As hemizygotes, the other four alleles are predominantly larval/pupal lethals. Partial complementation is achieved by most interallelic heterozygotes involving these four alleles. Phenotypic defects associated with the six tubulin mutation include disrupted embryos, pseudopupae, pharate adults with defects in various cuticular pattern elements, pharate adults with retarded head development, adults with leg tremors and extremely short life spans, and viable but sterile adults with bristle defects.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Matthews KA,Kaufman TCdoi
10.1016/0012-1606(87)90211-9subject
Has Abstractpub_date
1987-01-01 00:00:00pages
100-14issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(87)90211-9journal_volume
119pub_type
杂志文章abstract：:Meckel-Gruber syndrome (MKS) is an embryonic lethal ciliopathy resulting from mutations in genes encoding proteins localising to the primary cilium. Mutations in the basal body protein MKS1 account for 7% of cases of MKS. The condition affects the development of multiple organs, including brain, kidney and skeleton. H...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.02.015
更新日期：2013-05-01 00:00:00
abstract：:In the zebrafish embryo, cells fated to give rise to the rostral brain move in a concerted fashion and retain tissue coherence during morphogenesis. We demonstrate here that Otx proteins have a dramatic effect on cell-cell interactions when expressed ectopically in the zebrafish embryo. Injection of zebrafish Otx1 or ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9771
更新日期：2000-07-15 00:00:00
abstract：:These studies were designed to test the hypothesis that sperm nuclear decondensation and male pronuclear formation during hamster fertilization depend upon the ability of the fertilized oocyte to reduce sperm nuclear disulfide bonds. In a first series of experiments, treatment of mature oocytes with the sulfhydryl blo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90126-x
更新日期：1984-01-01 00:00:00
abstract：:Although a large superfamily of G-protein-coupled receptors serves multiple functions, little is known about their functional activation during ontogeny. To examine the functional activation of the mu-opioid receptor (MOR) and the delta-opioid receptor (DOR) during development, sections of mouse embryos and fetuses fr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0855
更新日期：2003-01-01 00:00:00
abstract：:We studied the pathway of 5S ribosomal RNA (rRNA) during oogenesis in Xenopus from its storage in the cytoplasm to incorporation into ribosomes in the nucleus. Ribonucleoprotein particle (RNP) assembly assays reveal striking differences in the behavior of oocyte-type and somatic-type 5S rRNA after microinjection into ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1080
更新日期：1995-04-01 00:00:00
abstract：:The cortex and subcortical regions of the developing follicles and eggs of silkmoths are rich in cytoskeletal elements, particularly actin. In situ analysis using [3H]-polyuridylic acid and biotinylated oligo d(T) reveals a pattern of changes in poly(A)+ RNA distribution during oogenesis. The developing pattern of dis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1031
更新日期：1993-02-01 00:00:00
abstract：:The mab-21 gene was first identified because of its requirement for ray identity specification in Caenorhabditis elegans. It is now known to constitute a family of genes that are highly conserved from vertebrates to invertebrates, and two homologues Mab21l1 and Mab21l2 have been identified in many species. Here we des...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.07.016
更新日期：2004-10-15 00:00:00
abstract：:Gnathostomes (jawed vertebrates) possess skeletal muscles with unique functional and developmental features that are absent from cyclostomes-i.e., lamprey and hagfish. These gnathostome-specific traits include the epaxial and hypaxial division of myotomes, paired fin/limb muscles, shoulder girdle muscles, and the musc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.10.029
更新日期：2011-02-01 00:00:00
abstract：:The effect of hydrocortisone on the development of dorsal skin was analyzed in the chick embryo by (1) transmission electron microscopy, (2) indirect immunofluorescence histology of extracellular matrix components (collagen types I, III, and IV; fibronectin; and laminin), and (3) quantitative determination of collagen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90056-3
更新日期：1984-11-01 00:00:00
abstract：:Inhibitor of differentiation and DNA-binding (Id) proteins, Id1 to Id4, function in the regulation of cellular proliferation and differentiation. Id proteins have been shown to interact with bHLH proteins and other proteins involved in regulating cellular proliferation and differentiation, suggesting a widespread regu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.12.006
更新日期：2020-04-15 00:00:00
abstract：:Trypsin-like activity is secreted from eggs of many species at fertilization, and this activity is believed to be critical for the block to polyspermy. Here we show that a cortical granule serine protease of sea urchins is the major and perhaps only protease family member important for fertilization. Zymography assays...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9299
更新日期：1999-07-01 00:00:00
abstract：:Sequence analysis of cDNA clones encoding fibronectin (FN) from Xenopus laevis reveals extensive amino acid identities with other vertebrate FNs, including the presence of the Arg-Gly-Asp (RGD) cell attachment site in type III-10 and of a second, cell-binding site (EILDV) in the alternative spliced V region of the pro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90291-n
更新日期：1992-02-01 00:00:00
abstract：:Members of the fibroblast growth factor (FGF) family of peptide growth factors are widely expressed in the germ layer derivatives during gastrulation and early organogenesis of the mouse. We have investigated the effect of administering recombinant FGF-4 in the late-primitive streak stage embryo to test if the pattern...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9663
更新日期：2000-05-01 00:00:00
abstract：:The olfactory epithelium (OE) of the mouse provides a unique system for understanding how cell birth and cell death interact to regulate neuron number during development and regeneration. We have examined cell death in the OE in normal adult mice; in adult mice subjected to unilateral olfactory bulbectomy (surgical re...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.0025
更新日期：1995-11-01 00:00:00
abstract：:The expression of the neural cell adhesion molecules N-CAM and L1 was investigated in the olfactory system of the mouse using immunocytochemical and immunochemical techniques. In the olfactory epithelium, globose basal cells and olfactory neurons were stained by the polyclonal N-CAM antibody reacting with all three co...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90397-1
更新日期：1988-10-01 00:00:00
abstract：:Three dominant second-chromosome rearrangement mutations in Drosophila melanogaster, Aristapedioid1 (Arp), vestigial-Depilate (vgD), and vestigial62 (vg62), result in developmental abnormalities of the bristle sense organs on the notum, abdomen, legs, and wing margin. The bristle abnormalities are associated with over...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1039
更新日期：1994-02-01 00:00:00
abstract：:Little is known about the biochemical changes underlying the morphological differentiation of the sea urchin egg during oogenesis. Because of this and the essential role of gamete recognition in fertilization, we studied the developmental expression of the recently identified egg surface receptor for sperm during ooge...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1233
更新日期：1994-09-01 00:00:00
abstract：:Oocyte maturation in mouse is associated with a dramatic reorganisation of the endoplasmic reticulum (ER) from a network of cytoplasmic accumulations in the germinal vesicle-stage oocyte (GV) to a network of distinctive cortical clusters in the metaphase II egg (MII). Multiple lines of evidence suggest that this redis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.02.006
更新日期：2007-05-01 00:00:00
abstract：:Large steady electrical currents traverse Drosophila follicles in vitro as well as permeabilized eggs. During the period of main follicle growth (stages 9-11), these currents enter the anterior or nurse cell end of the follicles. This inward current acts like a sodium ion influx with some calcium involvement. During t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90013-2
更新日期：1985-03-01 00:00:00
abstract：:TGF-beta (TGFβ) family mediated Smad signaling is involved in mesoderm and endoderm specifications, left-right asymmetry formation and neural tube development. The TGFβ1/2/3 and Activin/Nodal signal transduction cascades culminate with activation of SMAD2 and/or SMAD3 transcription factors and their overactivation are...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.09.025
更新日期：2014-12-01 00:00:00
abstract：:We investigated the potential role of rostral-caudal and dorsal-ventral subdivisions of the early rostral brain by relating these subdivisions to the early patterning of neuron cell bodies and their axon projections. The earliest neurons were mapped using the lipophilic axon tracers diI and diO on embryos fixed on emb...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0008
更新日期：1996-01-10 00:00:00
abstract：:Missense mutations in the MYH3 gene encoding myosin heavy chain-embryonic (MyHC-embryonic) have been reported to cause two skeletal muscle contracture syndromes, Freeman Sheldon Syndrome (FSS) and Sheldon Hall Syndrome (SHS). Two residues in MyHC-embryonic that are most frequently mutated, leading to FSS, R672 and T17...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.02.017
更新日期：2019-05-15 00:00:00
abstract：:The current gene regulatory network (GRN) for the sea urchin embryo pertains to pregastrular specification functions in the endomesodermal territories. Here we extend gene regulatory network analysis to the adjacent oral and aboral ectoderm territories over the same period. A large fraction of the regulatory genes pre...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.02.029
更新日期：2009-05-15 00:00:00
abstract：:Hedgehog (Hh) proteins and cAMP-dependent protein kinase A (PKA) generally play opposing roles in developmental patterning events. Humans and mice heterozygous for mutations in the sonic hedgehog (Shh) receptor gene patched-1 (ptc1) have an increased incidence of certain types of cancer, including medulloblastoma (MB)...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.10.031
更新日期：2008-01-01 00:00:00
abstract：:Metamorphosis in Drosophila melanogaster is orchestrated by the steroid hormone ecdysone, which triggers a cascade of primary-response transcriptional regulators and secondary effector genes during the third larval instar and prepupal periods of development. The early ecdysone-response Broad-Complex (BR-C) gene, a key...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9879
更新日期：2000-11-01 00:00:00
abstract：:Although round spermatid injection can be used to create progeny for males who do not produce mature sperm, the rate of successful embryogenesis after such procedures is significantly lower than that for similar procedures using mature spermatozoa. The mechanisms underlying this difference are unknown. In this study, ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.10.026
更新日期：2006-01-01 00:00:00
abstract：:The enteric nervous system (ENS) of the larval moth Manduca sexta consists of two small ganglia and several nerve networks that lie superficially along the alimentary tract. Within this system are approximately 600 neurons that exhibit a spectrum of biochemical and morphological characteristics and that express these ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(89)80039-9
更新日期：1989-01-01 00:00:00
abstract：:Horseradish peroxidase (HRP) was used as an intracellular lineage tracer in two experiments designed to reveal the sites of origin of cells that formed the duplicate embryo which developed in relation to an organizer grafted in the ventral marginal zone (VMZ) of Xenopus laevis embryos. In the first experiment a dorsal...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90180-5
更新日期：1984-03-01 00:00:00
abstract：:Autoradiography has been used to confirm and to extend previous microspectrophotometric studies (Doerder and DeBault, 1975) on the timing of DNA synthesis during conjugation in Tetrahymena thermophila. The majority of DNA synthesis occurs at the expected periods preceding gamete formation and the two postzygotic divis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90500-8
更新日期：1987-12-01 00:00:00
abstract：:We utilized a strain of mice, derived from a radiation mutagenesis experiment and carrying an activity-attenuated allele of the X-linked enzyme glucose-6-phosphate dehydrogenase (G6PD), to analyze the development of the cell lineage leading to cerebellar Purkinje neurons. Due to random X inactivation during early embr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0083
更新日期：1996-03-15 00:00:00
