Abstract:
:The cortex and subcortical regions of the developing follicles and eggs of silkmoths are rich in cytoskeletal elements, particularly actin. In situ analysis using [3H]-polyuridylic acid and biotinylated oligo d(T) reveals a pattern of changes in poly(A)+ RNA distribution during oogenesis. The developing pattern of distribution of actin filaments in the ooplasm closely resembles that of poly(A)+ RNA. RNA polymerase II is also associated with the cortical cytoskeleton. Destruction of the actin filaments in the developing oocytes by cytochalasin D randomizes the distribution of mRNA and causes the displacement of RNA polymerase II from the cortex. Rhodamine-conjugated phalloidin and a monoclonal antibody against cytoskeletal actin were used in combination with laser scanning confocal microscopy to examine the details of actin distribution in the oocytes. RNA polymerase II was located in developing oocytes using both anti-Drosophila RNA polymerase II antibody and fluorescein-conjugated amanitin.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Watson CA,Sauman I,Berry SJdoi
10.1006/dbio.1993.1031subject
Has Abstractpub_date
1993-02-01 00:00:00pages
315-23issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(83)71031-6journal_volume
155pub_type
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