Abstract：
:Actin-related protein 5 (ARP5) is a conserved subunit of the INO80 chromatin-remodeling complex in yeast and mammals. We have characterized the expression and subcellular distribution of Arabidopsis thaliana ARP5 and explored its role in the epigenetic control of multicellular development and DNA repair. ARP5-specific monoclonal antibodies localized ARP5 protein to the nucleoplasm of interphase cells in Arabidopsis and Nicotiana tabacum. ARP5 promoter-reporter fusions and the ARP5 protein are ubiquitously expressed. A null mutant and a severe knockdown allele produced moderately dwarfed plants with all organs smaller than the wild type. The small and slightly deformed organs such as leaves and hypocotyls were composed of small-sized cells. The ratio of leaf stomata to epidermal cells was high in the mutant, which also exhibited a delayed stomatal development compared with the wild type. Mutant plants were hypersensitive to DNA-damaging reagents including hydroxyurea, methylmethane sulfonate, and bleocin, demonstrating a role for ARP5 in DNA repair. Interestingly, the hypersensitivity phenotype of ARP5 null allele arp5-1 is stronger than the severe knockdown allele arp5-2. Moreover, a wild-type transgene fully complemented all developmental and DNA repair mutant phenotypes. Despite the common participation of both ARP4 and ARP5 in the INO80 complex, ARP4- and ARP5-deficient plants displayed only a small subset of common phenotypes and each displayed novel phenotypes, suggesting that in Arabidopsis they have both shared and unique functions.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Kandasamy MK,McKinney EC,Deal RB,Smith AP,Meagher RBdoi
10.1016/j.ydbio.2009.08.006subject
Has Abstractpub_date
2009-11-01 00:00:00pages
22-32issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(09)01115-4journal_volume
335pub_type
杂志文章abstract：:The Notch signaling pathway controls cell fate choices at multiple steps during cell lineage progression. To produce the cell fate choice appropriate for a particular stage in the cell lineage, Notch signaling needs to interpret the cell context information for each stage and convert it into the appropriate cell fate ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.09.018
更新日期：2010-12-15 00:00:00
abstract：:We have investigated the sequential changes of acetylcholine receptor (AChR) distribution on identified Xenopus laevis muscle cells in culture before and after innervation. AChRs on muscle cells were stained with tetramethylrhodamine-conjugated alpha-bungarotoxin and the distribution of AChR clusters was examined on a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90006-x
更新日期：1984-05-01 00:00:00
abstract：:Connexin 43 (Cx43) knockout mice and transgenic mice (CMV43) overexpressing the Cx43 gap junction gene exhibit heart defects involving the conotruncus and right ventricle. Based on the heart phenotype and Cx43 gene and transgene expression pattern, we previously proposed that the heart defects may reflect a role for g...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8891
更新日期：1998-06-01 00:00:00
abstract：:During the process of gastrulation in the sea urchin embryo, the vegetal plate invaginates to form the archenteron, a long narrow tube that extends across the blastocoel. Rearrangement of cells within the archenteron is thought to be a key component of the process of archenteron elongation. While these cell rearrangem...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1154
更新日期：1994-06-01 00:00:00
abstract：:The outgrowth of motor axons to the developing pectoral fin of the Japanese medaka fish (Oryzias latipes) was investigated both in wildtype embryos and in the pectoral finless (pl) mutants in which adults are missing pectoral fins. Late in embryogenesis the pectoral fin is a simple limb which contains two antagonist m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90445-9
更新日期：1991-07-01 00:00:00
abstract：:Vasa is a DEAD-box RNA helicase that functions in translational regulation of specific mRNAs. In many animals it is essential for germ line development and may have a more general stem cell role. Here we identify vasa in two sea urchin species and analyze the regulation of its expression. We find that vasa protein acc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.11.039
更新日期：2008-02-15 00:00:00
abstract：:MHM is a chicken Z chromosome-linked locus that is methylated and transcriptionally silent in male cells, but is hypomethylated and transcribed into a long non-coding RNA in female cells. MHM has been implicated in both localised dosage compensation and sex determination in the chicken embryo, but direct evidence is l...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.03.025
更新日期：2012-06-15 00:00:00
abstract：:The interaction of heterologous tissues involves cell adhesion mediated by the extracellular matrix and its receptor integrins. The Drosophila wing disc is an ectodermal invagination that contacts specific tracheal branches at the basolateral cell surface. We show that an alpha subunit of laminin, encoded by wing blis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.12.022
更新日期：2007-04-01 00:00:00
abstract：:In the full grown oocyte of Xenopus laevis, a sodium permeability can be induced by depolarization to positive potential with current injection. Voltage-clamp analysis has shown that depolarization causes a long-lasting modification of the membrane during which voltage-gated sodium channels become functional. In the p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90302-0
更新日期：1983-10-01 00:00:00
abstract：:Vertebrate hematopoiesis is characterized by two evolutionally conserved phases of development, i.e., primitive hematopoiesis, which is a transient phenomenon in the early embryo, and definitive hematopoiesis, which takes place in the later stages. Beni fuji (bef) was originally isolated as a medaka mutant that has an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.06.031
更新日期：2010-09-15 00:00:00
abstract：:Previous work has documented the importance of BMPs in eye development. Loss-of-function studies in mice, with targeted deletions in either the Bmp7 or Bmp4 genes, have shown that these molecules are critical for early eye development. On the basis of the asymmetry in the dorsal-ventral expression patterns of several ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00115-x
更新日期：2003-04-01 00:00:00
abstract：:Integrin α5-null embryos die in mid-gestation from severe defects in cardiovascular morphogenesis, which stem from defective development of the neural crest, heart and vasculature. To investigate the role of integrin α5β1 in cardiovascular development, we used the Mesp1(Cre) knock-in strain of mice to ablate integrin ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.09.014
更新日期：2014-11-15 00:00:00
abstract：:Although skeletal muscles appear superficially alike at different anatomical locations, in reality there is considerably more diversity than previously anticipated. Heterogeneity is not only restricted to completely developed fibers, but is clearly apparent during development at the molecular, cellular and anatomical ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2007.06.006
更新日期：2007-08-15 00:00:00
abstract：:In a previous study (K.-I. Sato et al., 1999, Dev. Biol. 209, 308-320), we presented evidence that a Src-related protein-tyrosine kinase (PTK), named Xyk, may act upstream of the calcium release in fertilization of the Xenopus egg. In the present study, we examined whether PTK activation of phospholipase Cgamma (PLCga...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9782
更新日期：2000-08-15 00:00:00
abstract：:The egg activation process functions to implement developmental programs that act much later in embryogenesis. One example of this is the fact that application of protein tyrosine kinase inhibitors to the fertilized sea urchin egg for a 15-min period results in a defect in the gastrulation process occurring over 24 h ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8743
更新日期：1998-01-01 00:00:00
abstract：:The formation of the spinal cord during early embryonic development in vertebrate embryos is a continuous process that begins at gastrulation and continues through to the completion of somitogenesis. Despite the conserved usage of patterning mechanisms and gene regulatory networks that act to generate specific spinal ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2017.01.021
更新日期：2017-12-01 00:00:00
abstract：:When a posterior fragment of the chick's marginal zone (PM) was exchanged with equal sized lateral marginal zone fragment (LM), of the same blastoderm, its capacity to initiate an ectopic primitive streak (PS) was found to be both size and stage dependent. Good correlation was demonstrated between the areas of PM frag...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90091-2
更新日期：1989-07-01 00:00:00
abstract：:Syndecan-3 is one of four identified members of a family of transmembrane proteoglycans (the syndecans) that possess highly similar cytoplasmic and transmembrane domains and may function as extracellular matrix receptors and/or low affinity receptors for signaling molecules such as FGF. We previously reported the clon...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1093
更新日期：1995-04-01 00:00:00
abstract：:Previous studies in sea urchin embryos have demonstrated that nuclearization of beta-catenin is essential for initial steps in the specification of endoderm and mesenchyme, which are derived from vegetal blastomeres. This process begins at the 4th and extends through the 9th cleavage stage, an interval in which the Sp...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00331-2
更新日期：2003-09-15 00:00:00
abstract：:Multipotent, self-renewing neural stem cells reside in the embryonic mouse telencephalic germinal zone. Using an in vitro neurosphere assay for neural stem cell proliferation, we demonstrate that FGF-responsive neural stem cells are present as early as E8.5 in the anterior neural plate, but EGF-responsive neural stem ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9192
更新日期：1999-04-01 00:00:00
abstract：:HP1 proteins are essential components of heterochromatin and contribute to the transcriptional repression of euchromatic genes. Although most species contain more than one HP1 family member which differ in their chromosomal distribution, it is not known to what extent the activity of these different family members is ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.06.039
更新日期：2006-10-01 00:00:00
abstract：:The control of cell division is critical to organogenesis, but how this control is achieved is not fully understood. We found that mutations in bed-3, encoding a BED Zn-finger domain transcription factor, confer a phenotype where a specific set of cell divisions during vulval organogenesis is lost. Unlike general cell...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.12.005
更新日期：2010-02-15 00:00:00
abstract：:Integrins are expressed on the surface of some vertebrate eggs where they are thought to have a role in fertilization. The objective of this study is to determine if integrins are expressed on sea urchin eggs. The alphaB and betaC subunits were cloned using the homology polymerase chain reaction. Monoclonal and polycl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9910
更新日期：2000-11-15 00:00:00
abstract：:Vertebrate Cdx genes encode homeodomain transcription factors related to caudal in Drosophila. The murine Cdx homologues Cdx1, Cdx2 and Cdx4 play important roles in anterior-posterior patterning of the embryonic axis and the intestine, as well as axial elongation. While our understanding of the ontogenic programs requ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.09.034
更新日期：2012-01-01 00:00:00
abstract：:The relative transcription of three unlinked actin genes of the sea urchin Strongylocentrotus purpuratus was measured in isolated nuclei, at several stages during embryonic development. Transcription of two cytoskeletal actin genes, CyI and CyIIIa, was first detected in 64-128 cell embryos. At the early stages, the Cy...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90473-8
更新日期：1987-11-01 00:00:00
abstract：:Several genomic clones were isolated from a Drosophila library screened with cDNA prepared from abundant adult female mRNA. Cytoplasmic dot hybridizations have shown that the genes in all of these clones are expressed only in posteclosion (stages 8-14) follicles. One set of overlapping clones (lambda 20, lambda 28, an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90271-9
更新日期：1984-09-01 00:00:00
abstract：:Thyroid hormone is a major regulator of postnatal brain development, but the precise molecular mechanisms underlying its action in this organ remain poorly understood. We used microarray analysis to identify new target genes in brain. Thyroid hormone treatment of hypothyroid Pax8(-/-) knockout mice, which lack thyroid...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00039-8
更新日期：2003-02-15 00:00:00
abstract：:Fetomodulin (FM) was previously shown to be a surface marker protein of parietal endoderm by in vitro differentiation of F9 embryonal carcinoma cells and by immunohistochemistry of in vivo embryos. BALB/3T3 and sarcoma S180 cells of the mouse were also shown to possess a protein which was indistinguishable from FM by ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90058-q
更新日期：1990-07-01 00:00:00
abstract：:Notch activation inhibits neuronal differentiation during development of the nervous system; however, the dynamic role of Notch signaling in individual cell lineages remains poorly understood. We have characterized 3.4 kb 5'-regulatory sequence of a Notch target gene, her4, and used it to drive fluorescent gene expres...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.10.020
更新日期：2007-01-15 00:00:00
abstract：:Fibronectin (Fn) plays an important part in the branching morphogenesis of salivary gland, lung, and kidney. Here, we examine the effect of the conditional knockout of Fn in the mammary epithelium [Fn(MEp-/-)] on postnatal mammary gland development, using Cre-loxP-mediated gene knockout technology. Our data show that ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.07.001
更新日期：2010-10-01 00:00:00
