Abstract:
:Multipotent, self-renewing neural stem cells reside in the embryonic mouse telencephalic germinal zone. Using an in vitro neurosphere assay for neural stem cell proliferation, we demonstrate that FGF-responsive neural stem cells are present as early as E8.5 in the anterior neural plate, but EGF-responsive neural stem cells emerge later in development in a temporally and spatially specific manner. By separately blocking EGF and FGF2 signaling, we also show that EGF alone and FGF2 alone can independently elicit neural stem cell proliferation and at relatively high cell densities separate cell nonautonomous effects can substantially enhance the mitogen-induced proliferation. At lower cell densities, neural stem cell proliferation is additive in the presence of EGF and FGF2 combined, revealing two different stem cell populations. However, both FGF-responsive and EGF-responsive neural stem cells retain their self-renewal and multilineage potential, regardless of growth factor conditions. These results support a model in which separate, lineage-related EGF- and FGF-responsive neural stem cells are present in the embryonic telencephalic germinal zone.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Tropepe V,Sibilia M,Ciruna BG,Rossant J,Wagner EF,van der Kooy Ddoi
10.1006/dbio.1998.9192subject
Has Abstractpub_date
1999-04-01 00:00:00pages
166-88issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(98)99192-8journal_volume
208pub_type
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