Abstract：
:The universal signal for egg activation at fertilization is a rise in cytoplasmic Ca(2+) with defined spatial and temporal kinetics. Mammalian and amphibian eggs acquire the ability to produce such Ca(2+) signals during a maturation period that precedes fertilization and encompasses resumption of meiosis and progression to metaphase II. In Xenopus, immature oocytes produce fast, saltatory Ca(2+) waves that can be oscillatory in nature in response to IP(3). In contrast, mature eggs produce a single continuous, sweeping Ca(2+) wave in response to IP(3) or sperm fusion. The mechanisms mediating the differentiation of Ca(2+) signaling during oocyte maturation are not well understood. Here, I characterized elementary Ca(2+) release events (Ca(2+) puffs) in oocytes and eggs and show that the sensitivity of IP(3)-dependent Ca(2+) release is greatly enhanced during oocyte maturation. Furthermore, Ca(2+) puffs in eggs have a larger spatial fingerprint, yet are short lived compared to oocyte puffs. Most interestingly, Ca(2+) puffs cluster during oocyte maturation resulting in a continuum of Ca(2+) release sites over space in eggs. These changes in the spatial distribution of elementary Ca(2+) release events during oocyte maturation explain the continuous nature and slower speed of the fertilization Ca(2+) wave.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Machaca Kdoi
10.1016/j.ydbio.2004.08.004subject
Has Abstractpub_date
2004-11-01 00:00:00pages
170-82issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(04)00515-9journal_volume
275pub_type
杂志文章abstract：:Immature, Stage VI oocytes of Xenopus laevis fail to activate (i.e., to propagate a cortical reaction and elevate a fertilization envelope) when pricked or exposed to A23187. We determined the times during maturation when immature oocytes treated with progesterone in vitro developed the capacity to respond to pricking...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90177-5
更新日期：1984-03-01 00:00:00
abstract：:Cell division is arguably the most fundamental developmental process for single-celled and multicellular organisms alike. The pathway from one cell division to the next is known as the cell cycle. A conserved biochemical regulatory network controls progress along this pathway in plants, animals, and yeasts. This revie...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/0012-1606(92)90087-w
更新日期：1992-09-01 00:00:00
abstract：:Genetic interactions regulating intermediate stages of tubulogenesis in the developing kidney have been difficult to define. A systems biology strategy using microarray was combined with in vitro/ex vivo and genetic approaches to identify pathways regulating specific stages of tubulogenesis. Analysis of the progressio...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.01.014
更新日期：2012-04-01 00:00:00
abstract：:In the zebrafish embryo, cells fated to give rise to the rostral brain move in a concerted fashion and retain tissue coherence during morphogenesis. We demonstrate here that Otx proteins have a dramatic effect on cell-cell interactions when expressed ectopically in the zebrafish embryo. Injection of zebrafish Otx1 or ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9771
更新日期：2000-07-15 00:00:00
abstract：:Cell adhesion molecules (CAMs) perform numerous functions during neural development. An individual CAM can play different roles during each stage of neuronal differentiation; however, little is known about how such functional switching is accomplished. Here we show that Drosophila N-cadherin (CadN) is required at mult...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.04.006
更新日期：2012-06-15 00:00:00
abstract：:Normal growth and morphogenesis of the cranial vault reflect a balance between cell proliferation in the sutures and osteogenesis at the margins of the cranial bones. In the clinical condition craniosynostosis, the sutures fuse prematurely as a result of precocious osteogenic differentiation and craniofacial malformat...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0533
更新日期：2002-03-01 00:00:00
abstract：:The Mesp bHLH genes play a conserved role during segmental patterning of the mesoderm in the vertebrate embryo by specifying segmental boundaries and anteroposterior (A-P) segmental polarity. Here we use a xenotransgenic approach to compare the transcriptional enhancers that drive expression of the Mesp genes within s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.12.038
更新日期：2008-03-15 00:00:00
abstract：:Myoblasts of the L6 rat cell line were grown in Ham's F12 nutrient medium containing 10% fetal calf serum (F12 + FCS). Although the cells were confluent by 6 days in culture, fusion was not observed even if cultures were maintained for 10-14 days. At least 80% of the cells in such confluent unfused cultures were in th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90192-1
更新日期：1984-04-01 00:00:00
abstract：:The dorsal air sacs supply oxygen to the flight muscles of the Drosophila adult. This tracheal organ grows from an epithelial tube (the air sac primordium (ASP)) that arises during the third larval instar (L3) from a wing-disc-associated tracheal branch. Since the ASP is generated by a program of both morphogenesis an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.09.005
更新日期：2005-11-01 00:00:00
abstract：:Cell-cell fusion is a fundamental cellular process that is essential for development as well as fertilization. Myoblast fusion to form multinucleated skeletal muscle myotubes is a well studied, yet incompletely understood example of cell-cell fusion that is essential for formation of contractile skeletal muscle tissue...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.10.035
更新日期：2009-01-15 00:00:00
abstract：:Pioneer axons in insect legs are experimentally accessible model systems for the molecular identification and cellular localization of guidance cues regulating the path of axon growth. A detailed study of the Fe2 pioneer axons in the legs of the cockroach was performed to examine the diversity of guidance mechanisms. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8704
更新日期：1997-10-15 00:00:00
abstract：:During metamorphosis of insect epithelial monolayers, cells die, divide, and rearrange. In Drosophila undifferentiated diploid cells destined to form the adult cuticle of each abdominal segment segregate early in development from the surrounding polyploid larval epithelial cells of that segment as eight groups of dipl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.03.017
更新日期：2018-06-01 00:00:00
abstract：:Regulation of gene expression during early embryonic development in the rabbit was investigated by quantitative assay of firefly luciferase activity obtained by microinjection of three plasmid constructs using the regulatory region of polyomavirus promoter (PrPyV) with two different enhancer sequences (wild type or mu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1188
更新日期：1994-07-01 00:00:00
abstract：:Completion of cytokinesis, abscission, has been studied little despite the intensive studies of the onset and contractile mechanism of the earlier phases of division. It has been well documented that microtubule (MT) disruption before furrow stimulation prevents furrowing, while MT disruption after furrow stimulation ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9395
更新日期：1999-10-01 00:00:00
abstract：:External genital development begins with formation of paired genital swellings, which develop into the genital tubercle. Proximodistal outgrowth and axial patterning of the genital tubercle are coordinated to give rise to the penis or clitoris. The genital tubercle consists of lateral plate mesoderm, surface ectoderm,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0668
更新日期：2002-07-01 00:00:00
abstract：:We have examined the presence of protein kinase C in oocytes of Chaetopterus pergamentaceus and its role in the initiation of germinal vesicle breakdown (GVBD). First, we demonstrated that the oocytes contain a phospholipid- and calcium-dependent protein kinase, protein kinase C (PKC). Since PKC is the primary intrace...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90294-q
更新日期：1992-02-01 00:00:00
abstract：:Receptor protein tyrosine phosphatases (RPTPs) have been shown to play key roles in regulating axon guidance and synaptogenesis. HmLAR2, one of two closely related LAR-like RPTPs in the embryonic leech, is expressed in a few central neurons and in a unique segmentally-iterated peripheral cell, the comb cell (CC). Here...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.05.522
更新日期：2008-08-01 00:00:00
abstract：:Pigment cells of the zebrafish, Danio rerio, offer an exceptionally tractable system for studying the genetic and cellular bases of cell fate decisions. In the zebrafish, neural crest cells generate three types of pigment cells during embryogenesis: yellow xanthophores, iridescent iridophores and black melanophores. I...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.04.023
更新日期：2010-08-01 00:00:00
abstract：:Connexin 43 (Cx43) knockout mice and transgenic mice (CMV43) overexpressing the Cx43 gap junction gene exhibit heart defects involving the conotruncus and right ventricle. Based on the heart phenotype and Cx43 gene and transgene expression pattern, we previously proposed that the heart defects may reflect a role for g...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8891
更新日期：1998-06-01 00:00:00
abstract：:The extracellular matrix of Xenopus laevis oocytes was analyzed before and after meiotic maturation using quick-freeze, deep-etch, rotary-shadow electron microscopy. The perivitelline space (PS) of the meiotically immature oocyte contains a filamentous network which connects microvilli (MV) and follicle cell macrovill...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(89)80044-2
更新日期：1989-01-01 00:00:00
abstract：:Myotendinous junctions (MTJs) are specialized sites at the surface of muscle fibers where force is transmitted between muscle and tendon. Morphogenesis of MTJs in the latter half of embryonic chick development is characterized by formation of basement membrane, appearance of subsarcolemmal densities, association of my...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1161
更新日期：1994-06-01 00:00:00
abstract：:Extracellular modulators of cell-cell signaling control numerous aspects of organismal development. The Twisted gastrulation (Twsg1) gene product is a small, secreted cysteine-rich protein that has the unusual property of being able to either enhance or inhibit signaling by the bone morphogenetic protein (BMP) subfami...
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pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.11.015
更新日期：2004-03-15 00:00:00
abstract：:Chicken talpid(3) mutant embryos have a wide range of Hedgehog-signalling related defects and it is now known that the talpid(3) gene product encodes a novel protein essential for Hedgehog signalling which is required for both activator and repressor functions of Gli transcription factors (Davey, M.G., Paton, I.R., Yi...
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pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.08.017
更新日期：2007-01-01 00:00:00
abstract：:To understand cell interactions during induction of skin appendages, we studied the roles of adhesion molecules N-CAM, tenascin, integrin, and fibronectin during feather development. Tenascin appeared in a periodic pattern on epithelia and was so far the earliest molecule detected in placodes. Three placode domains we...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90009-6
更新日期：1992-03-01 00:00:00
abstract：:The effect of phenylephrine, an alpha-1 adrenergic agonist, on development of body asymmetry was studied using a rat whole embryo culture system. Embryos were explanted at the presomite stage, cultured in 100% rat serum containing various concentrations of phenylephrine, and examined at the 20-25 somite stage for side...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90067-d
更新日期：1991-01-01 00:00:00
abstract：:To investigate the spatiotemporal dynamics of skin pattern formation, we developed a simple method for artificially disarranging the placement of all three pigment cell types in the body trunk of zebrafish (Danio rerio). We generated transgenic fish with melanophores that ectopically expressed a variant of channelrhod...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.12.019
更新日期：2020-04-01 00:00:00
abstract：:Notch signaling has a fundamental role in stem cell maintenance and in cell fate choice in the intestine of different species. Canonically, Notch signaling represses the expression of transcription factors of the achaete-scute like (ASCL) or atonal related protein (ARP) families. Identifying the ARP/ASCL genes express...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.01.011
更新日期：2013-04-15 00:00:00
abstract：:The molecular and cellular mechanism for clearance of dead neurons was explored in the developing Drosophila optic lobe. During development of the optic lobe, many neural cells die through apoptosis, and corpses are immediately removed in the early pupal stage. Most of the cells that die in the optic lobe are young ne...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.05.003
更新日期：2019-09-01 00:00:00
abstract：:mid and H15 encode Tbx20 transcription factors that specify ventral pattern in the Drosophila leg. We find that there are at least two pathways for mid and H15 specification of ventral fate. In the first pathway, mid and H15 negatively regulate Dpp, the dorsal signal in leg development. mid and H15 block the dorsalizi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.05.012
更新日期：2019-11-01 00:00:00
abstract：:The development of germ cells is a highly ordered process that begins during fetal growth and is completed in the adult. Epigenetic modifications that occur in germ cells are important for germ cell function and for post-fertilization embryonic development. We have previously shown that male germ cells in the adult mo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.05.002
更新日期：2007-07-15 00:00:00
