Abstract：
:We have examined the presence of protein kinase C in oocytes of Chaetopterus pergamentaceus and its role in the initiation of germinal vesicle breakdown (GVBD). First, we demonstrated that the oocytes contain a phospholipid- and calcium-dependent protein kinase, protein kinase C (PKC). Since PKC is the primary intracellular receptor for phorbol esters, we tested the ability of phorbol 12,13-dibutyrate (PDBu) to induce GVBD and compared several critical events and processes involved in GVBD induced by PDBu to those induced normally (by seawater). Seawater and 100-200 nM PDBu induced chromosome condensation, spindle formation, and spindle migration over a similar time course. Both treatments induced similar alterations in the SDS-PAGE pattern of newly synthesized proteins. The synthesis of polypeptides of approximately 46 and 54 kDa increased specifically. Both treatments increased oocyte protein phosphorylation, especially of proteins of 22, 32, 46, 55, 64, and 84 kDa. Both treatments resulted in the activation of an M-phase-specific histone H1 kinase activity, which demonstrates the appearance of maturation-promoting factor. Staurosporine, a potent protein kinase C inhibitor, blocked GVBD and the activation of M-phase-specific H1 kinase, whereas HA1004, which preferentially antagonizes protein kinase A, had no effect. The results of this study demonstrate that protein kinase C can activate a wide spectrum of essential biochemical and morphological processes involved in GVBD. Further, these studies suggest that protein kinase C elicits GVBD by activating maturation-promoting factor and support the hypothesis that protein kinase C plays an essential role in oocyte maturation in this species.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Eckberg WR,Palazzo REdoi
10.1016/0012-1606(92)90294-qsubject
Has Abstractpub_date
1992-02-01 00:00:00pages
395-405issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(92)90294-Qjournal_volume
149pub_type
杂志文章abstract：:Brain 4 (Brn4/Pou3f4) and Pax6 are POU-homeodomain and paired-homeodomain transcription factors, respectively, that are expressed in the brain and the glucagon-expressing cells in the pancreas. Brn4 expression begins at embryonic day 10 in the pancreas, just before pax6 and both appear in the glucagon immunoreactive c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.12.008
更新日期：2004-04-01 00:00:00
abstract：:teashirt (tsh) encodes a zinc-finger protein that is thought to be part of a network that contributes to regionalization of the Drosophila embryo and establishes the domains of Hox protein function. tsh and the Hox gene Sex combs reduced (Scr) are essential to establish the identity of the first thoracic segment. We u...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.04.028
更新日期：2007-07-01 00:00:00
abstract：:We have investigated oocyte development in Hydra vulgaris, a member of one of the oldest metazoan phyla. We show that oocyte determination involves a mechanism that establishes a subset of precursor interstitial cells competent to differentiate into oocytes. The oocyte is singled out from this subset and the competenc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9790
更新日期：2000-08-15 00:00:00
abstract：:The left-right body axis is coordinately aligned with the orthogonal dorsoventral and anterioposterior body axes. The developmental mechanisms that regulate axis coordination are unknown. Here it is shown that the cardiac left-right orientation in zebrafish (Danio rerio) is randomized in notochord-defective no tail an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0148
更新日期：1996-07-10 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0572
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abstract：:VegT is an essential maternal regulator of germ layer specification in Xenopus. The localization of VegT mRNA to the vegetal cortex of the oocyte during oogenesis ensures its inheritance by vegetal and not animal cells, and directs the differentiation of vegetal cells into endoderm. Similarly localized mRNAs, Vg1 and ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0495
更新日期：2001-12-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.03.004
更新日期：2005-05-15 00:00:00
abstract：:The insect head is composed of several segments. During embryonic development, the segments fuse to form a rigid head capsule where obvious segmental boundaries are lacking. Hence, the assignment of regions of the insect head to specific segments is hampered, especially with respect to dorsal (vertex) and lateral (gen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.11.010
更新日期：2010-02-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1191
更新日期：1995-07-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2014-12-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1301
更新日期：1994-11-01 00:00:00
abstract：:Smad4 is defined as the common-mediator Smad (co-Smad) required for transducing signals for all TGF-beta superfamily members. This paper describes two Smad4s in Xenopus: XSmad4alpha, which is probably the Xenopus orthologue of human Smad4, and a distinct family member, XSmad4beta, which differs primarily at the extrem...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9430
更新日期：1999-10-15 00:00:00
abstract：:Trichosia pubescens larvae were ligated at different prepupal developmental stages in order to investigate whether ecdysterone acts just as a trigger or whether its presence in the hemolymph is constantly necessary for the induction of the late puffs, in particular the DNA puffs. The larvae were ligated in the middle ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90170-2
更新日期：1984-03-01 00:00:00
abstract：:Pax3 is expressed early during embryonic development in spatially restricted domains including limb muscle, neural crest, and neural tube. Pax3 functions at the nodal point in melanocyte stem cell differentiation, cardiogenesis and neurogenesis. Additionally Pax3 has been implicated in migration and differentiation of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.01.008
更新日期：2008-04-15 00:00:00
abstract：:The egg activation process functions to implement developmental programs that act much later in embryogenesis. One example of this is the fact that application of protein tyrosine kinase inhibitors to the fertilized sea urchin egg for a 15-min period results in a defect in the gastrulation process occurring over 24 h ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8743
更新日期：1998-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90049-1
更新日期：1985-04-01 00:00:00
abstract：:Genetic interactions regulating intermediate stages of tubulogenesis in the developing kidney have been difficult to define. A systems biology strategy using microarray was combined with in vitro/ex vivo and genetic approaches to identify pathways regulating specific stages of tubulogenesis. Analysis of the progressio...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.01.014
更新日期：2012-04-01 00:00:00
abstract：:Malfunctions of motile cilia cause a variety of developmental defects and diseases in humans and animal model organisms. Defects include impaired mucociliary clearance of the airways, sperm immotility, hydrocephalus and organ laterality. Here, we characterize the evolutionary conserved Cfap43 gene by loss-of-function ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.12.010
更新日期：2020-03-15 00:00:00
abstract：:Rapidly growing human teratocarcinoma cells (Tera-2) can be induced to differentiate into quiescent, nontumorigenic cells expressing neuronal markers. To more closely mimic the in vivo conditions for tumor growth, we grew Tera-2 cells in three-dimensional collagen gel cultures. The undifferentiated cells proliferated ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1016
更新日期：1994-01-01 00:00:00
abstract：:Embryonic development involves a series of cell adhesive interactions that provide mechanical and instructive information required for morphogenesis. The ADAMs family of membrane-anchored proteins, containing a disintegrin and metalloprotease domain, is well suited for participating in such developmental events. They ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.8458
更新日期：1997-02-15 00:00:00
abstract：:The developing chick limb bud contains several stage-specific populations of muscle colony-forming (MCF) cells. The cell lineage relationships of these populations are not completely understood, but previous studies have suggested that there may be at least two myogenic precursor populations present in the limb bud. T...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90237-9
更新日期：1984-12-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2007-06-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90056-0
更新日期：1989-06-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2000-02-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.06.003
更新日期：2019-09-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.01.025
更新日期：2009-04-15 00:00:00
abstract：:The desmin gene encodes an intermediate filament protein that is present in skeletal, cardiac, and smooth muscle cells. This study shows that the 4-kb upstream region of the murine desmin promoter directs expression of a lacZ reporter gene throughout the heart from E7.5 and in skeletal muscle and vascular smooth muscl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9865
更新日期：2000-10-15 00:00:00
abstract：:Specification of the trophectoderm (TE) and inner cell mass (ICM) lineages in the mouse blastocyst correlates with cell position, as TE derives from outer cells whereas ICM from inner cells. Differences in position are reflected by cell polarization and Hippo signaling. Only in outer cells, the apical-basal cell polar...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2014-10-01 00:00:00
abstract：:Gene transcription mediates many vital aspects of mammalian embryonic development. A comprehensive characterization and analysis of the dynamics of gene transcription in the embryo is therefore likely to provide significant insights into the basic mechanisms of this process. We used microarrays to map transcription in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.09.036
更新日期：2005-12-15 00:00:00
abstract：:Cranial placodes are specialized ectodermal regions in the developing vertebrate head that give rise to both neural and non-neural cell types of the neuroendocrine system and the sense organs of the visual, olfactory and acoustic systems. The cranial placodes develop from a panplacodal region which is specifically mar...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2011-02-01 00:00:00
