Abstract:
:mid and H15 encode Tbx20 transcription factors that specify ventral pattern in the Drosophila leg. We find that there are at least two pathways for mid and H15 specification of ventral fate. In the first pathway, mid and H15 negatively regulate Dpp, the dorsal signal in leg development. mid and H15 block the dorsalizing effects of Dpp signaling in the ventral leg. In loss- and gain-of-function experiments in imaginal discs, we show that mid and H15 block the accumulation of phospho-Mad, the activated form of the Drosophila pSmad1/5 homolog. In a second pathway, we find mid and H15 must also directly promote ventral fate because simultaneously blocking Dpp signaling in mid H15 mutants does not rescue the ventral to dorsal transformation in most ventral leg structures. We show that mid and H15 act as transcriptional repressors in ventral leg development. The two genes repress the Dpp target gene Dad, the laterally expressed gene Upd, and the mid VLE enhancer. This repression depends on the eh1 domain, a binding site for the Groucho co-repressor, and is likely direct because Mid localizes to target gene enhancers in PCR-ChIP assays. A mid allele mutant for the repressing domain (eh1), mideh1, was found to be compromised in gain-of-function assays and in rescue of mid H15 loss-of-function. We propose that mid and H15 specify ventral fate through inhibition of Dpp signaling and through coordinating the repression of genes in the ventral leg.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Svendsen PC,Phillips LA,Deshwar AR,Ryu JR,Najand N,Brook WJdoi
10.1016/j.ydbio.2019.05.012subject
Has Abstractpub_date
2019-11-01 00:00:00pages
19-31issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(18)30607-9journal_volume
455pub_type
杂志文章abstract::The Wnt pathways are evolutionarily well-conserved signal transduction pathways that are known to play important roles in all Metazoans investigated to date. Here, we examine the Wnt pathway genes and target genes present in the genome of the echinoderm Strongylocentrotus purpuratus. Analysis of the Wnt genes revealed...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.08.045
更新日期:2006-12-01 00:00:00
abstract::FOG-2 is a multi-zinc finger protein that binds the transcriptional activator GATA4 and modulates GATA4-mediated regulation of target genes during heart development. Our previous work has demonstrated that the Nucleosome Remodeling and Deacetylase (NuRD) complex physically interacts with FOG-2 and is necessary for FOG...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.08.030
更新日期:2014-11-01 00:00:00
abstract::Adhesive extracellular matrix (ECM) molecules appear to play roles in the migration of neural crest cells, and may also provide cues for differentiation of these cells into a variety of phenotypes. We are studying the influences of specific ECM components on crest differentiation at the levels of both individual cells...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90112-v
更新日期:1990-09-01 00:00:00
abstract::Clotting is critical in limiting hemolymph loss and initiating wound healing in insects as in vertebrates. It is also an important immune defense, quickly forming a secondary barrier to infection, immobilizing bacteria and thereby promoting their killing. However, hemolymph clotting is one of the least understood immu...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.03.019
更新日期:2006-07-01 00:00:00
abstract::Asymmetric cell divisions produce all 302 neurons of the C. elegans hermaphrodite. Here, we describe a role for a C. elegans Dishevelled homolog, DSH-2, in an asymmetric neuroblast division. In dsh-2 mutants, neurons normally descended from the anterior neuroblast daughter of the ABpl/rpppa blast cell were frequently ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.05.024
更新日期:2005-08-01 00:00:00
abstract::The development of supernumerary bristle precursors induced by the mutation shaggy (sgg; also known as zeste-white 3) was examined in the developing wing blade of imaginal and pupal Drosophila. sgg clones were induced by mitotic recombination; clones were marked using enhancer-trap flies which express beta-galactosida...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90134-3
更新日期:1992-08-01 00:00:00
abstract::etv2 is an endothelial-specific ETS transcription factor that is essential for vascular differentiation and morphogenesis in vertebrates. While recent data suggest that Etv2 is dynamically regulated during vascular development, little is known about the mechanisms involved in this process. Here, we find that etv2 tran...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.08.028
更新日期:2013-12-01 00:00:00
abstract::To examine the relationship between growth hormone (GH) and insulin-like growth factor 1 (IGF1) in controlling postnatal growth, we performed a comparative analysis of dwarfing phenotypes manifested in mouse mutants lacking GH receptor, IGF1, or both. This genetic study has provided conclusive evidence demonstrating t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9975
更新日期:2001-01-01 00:00:00
abstract::It is known that secreted proteins from the anterior lateral endoderm, FGF8 and BMP2, are involved in mesodermal cardiac differentiation, which determines the first cardiac field, defined by the expression of the earliest specific cardiac markers Nkx-2.5 and Gata4. However, the molecular mechanisms responsible for ear...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.07.007
更新日期:2015-10-01 00:00:00
abstract::Using gene-specific probes derived from four mouse gamma-crystallin cDNAs, we have examined the regulation of different members of the mouse gamma-crystallin gene family during lens development. Our analysis revealed that, while the different gamma-crystallin genes appear to be coordinately activated during embryogene...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90227-2
更新日期:1987-01-01 00:00:00
abstract::In adult vertebrates, fibroblast growth factor (FGF) synergizes with many hematopoietic cytokines to stimulate the proliferation of hematopoietic progenitors. In vertebrate development, the FGF signaling pathway is important in the formation of some derivatives of ventroposterior mesoderm. However, the function of FGF...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9205
更新日期:1999-04-15 00:00:00
abstract::Ooplasmic segregation in the late interphase zygote of the leech Theromyzon trizonare is accomplished by reorganization of an ectoplasmic cytoskeleton formed by polar rings and meridional bands. The dynamic properties of this cytoskeleton were explored by time-lapse confocal and video microscopy. Cytoskeleton assembly...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.03.023
更新日期:2004-07-01 00:00:00
abstract::We examined the structure, expression, and possible functions of a nanos homologue during gametogenesis and blastogenesis in the colonial ascidian Botryllus primigenus. An approximately 1.3-kb-long cDNA was cloned; it was termed BpNos since the deduced amino acid sequence (288 aa) contained 2 Nanos-like CCHC zinc fing...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.08.023
更新日期:2008-12-01 00:00:00
abstract::Integrin α5-null embryos die in mid-gestation from severe defects in cardiovascular morphogenesis, which stem from defective development of the neural crest, heart and vasculature. To investigate the role of integrin α5β1 in cardiovascular development, we used the Mesp1(Cre) knock-in strain of mice to ablate integrin ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.09.014
更新日期:2014-11-15 00:00:00
abstract::Nuclear receptors (NRs) are a large class of ligand-regulated transcriptional modulators that have been shown to play roles in many developmental processes. The Caenorhabditis elegans genome is predicted to encode a large and divergent family of NR proteins. The functions of most of these genes are unknown. As a first...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9470
更新日期:1999-11-15 00:00:00
abstract::Vertebrate voluntary muscles are composed of myotubes and connective tissue cells. These two cell types have different embryonic origins: myogenic cells arise from paraxial mesoderm, while in the head many of the connective tissues are formed by neural crest cells. The objective of this research was to study interacti...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90138-7
更新日期:1986-08-01 00:00:00
abstract::In the full grown oocyte of Xenopus laevis, a sodium permeability can be induced by depolarization to positive potential with current injection. Voltage-clamp analysis has shown that depolarization causes a long-lasting modification of the membrane during which voltage-gated sodium channels become functional. In the p...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90302-0
更新日期:1983-10-01 00:00:00
abstract::An important developmental question concerns whether neurotransmitter phenotype is an inherent property of neurons or is influenced by target tissues. This issue can be addressed in the avian ciliary ganglion (CG) which contains two cholinergic populations, ciliary and choroid neurons, that differentially express the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8998
更新日期:1998-10-15 00:00:00
abstract::The zebrafish hedgehog (hh) family members tiggy-winkle hedgehog (twhh) and sonic hedgehog (shh) are involved in patterning the ventral CNS and proximal eye. Using a dominant negative protein kinase A regulatory subunit mutant, we show that these hh activities are mediated by protein kinase A. The effects of dominant ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0209
更新日期:1996-08-25 00:00:00
abstract::Procedures are described for removal of the vitelline membrane from gently fixed Drosophila embryos en masse. The resulting embryos retain excellent structural integrity and are now suitable for a variety of immunocytochemical and biochemical characterizations. ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90275-0
更新日期:1983-09-01 00:00:00
abstract::A monoclonal antibody, Mab 8A2, that recognizes a novel set of gangliosides was produced by immunizing a mouse with Embryonic Day 14 chick optic nerve. Immunohistochemical studies of the developing chick retina revealed a complex pattern of Mab 8A2 immunoreactivity. Initially, staining is concentrated in the optic fib...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90221-n
更新日期:1991-05-01 00:00:00
abstract::We report here the identification and characterization of a novel gene, T1 alpha, expressed in high abundance in adult rat lung, fetal lung, and early fetal brain. T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. The cDNA for...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1024
更新日期:1995-01-01 00:00:00
abstract::Caveolin-1 (Cav-1) is the principal component of plasma membrane caveolae that negatively regulates a number of cellular signaling events including canonical Wnt signaling. Activation of the Wnt/beta-catenin pathway is essential for dorsal organizer formation and specification in early vertebrate embryos, but it remai...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.04.033
更新日期:2010-08-01 00:00:00
abstract::A cDNA library was used to measure changes in many individual mRNAs during muscle differentiation in culture. A library of 1000 clones was constructed from total myofiber poly(A) RNA. About 23% of these clones gave a detectable colony hybridization signal using end-labeled myofiber mRNA, the remainder containing muscl...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90049-0
更新日期:1983-02-01 00:00:00
abstract::Proper patterning and growth of oral structures including teeth, tongue, and palate rely on epithelial-mesenchymal interactions involving coordinated regulation of signal transduction. Understanding molecular mechanisms underpinning oral-facial development will provide novel insights into the etiology of common congen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.05.002
更新日期:2011-08-01 00:00:00
abstract::Myotendinous junctions (MTJs) are specialized sites at the surface of muscle fibers where force is transmitted between muscle and tendon. Morphogenesis of MTJs in the latter half of embryonic chick development is characterized by formation of basement membrane, appearance of subsarcolemmal densities, association of my...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1161
更新日期:1994-06-01 00:00:00
abstract::Members of the fibroblast growth factor (FGF) family of peptide growth factors are widely expressed in the germ layer derivatives during gastrulation and early organogenesis of the mouse. We have investigated the effect of administering recombinant FGF-4 in the late-primitive streak stage embryo to test if the pattern...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9663
更新日期:2000-05-01 00:00:00
abstract::Spore germination is a defined developmental process that marks a critical point in the life cycle of Dictyostelium discoideum. Upon germination the environmental conditions must be conducive to cell growth to ensure survival of emerged amoebae. However, the signal transduction pathways controlling the various aspects...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8854
更新日期:1998-04-15 00:00:00
abstract::It is well established that the notochord influences the development of adjacent neural and mesodermal tissue. Involvement of the notochord in the differentiation of the dorsal pancreas has been demonstrated. However, our knowledge of the signals involved in pancreatic development is still incomplete. In order to iden...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9348
更新日期:1999-08-15 00:00:00
abstract::Auxin is implicated throughout plant growth and development. Although the effects of this plant hormone have been recognized for more than a century, it is only in the past two decades that light has been shed on the molecular mechanisms that regulate auxin homeostasis, signaling, transport, crosstalk with other hormo...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2012.05.039
更新日期:2012-09-01 00:00:00