Abstract：
:Proper patterning and growth of oral structures including teeth, tongue, and palate rely on epithelial-mesenchymal interactions involving coordinated regulation of signal transduction. Understanding molecular mechanisms underpinning oral-facial development will provide novel insights into the etiology of common congenital defects such as cleft palate. In this study, we report that ablating Wnt signaling in the oral epithelium blocks the formation of palatal rugae, which are a set of specialized ectodermal appendages serving as Shh signaling centers during development and niches for sensory cells and possibly neural crest related stem cells in adults. Lack of rugae is also associated with retarded anteroposterior extension of the hard palate and precocious mid-line fusion. These data implicate an obligatory role for canonical Wnt signaling in rugae development. Based on this complex phenotype, we propose that the sequential addition of rugae and its morphogen Shh, is intrinsically coupled to the elongation of the hard palate, and is critical for modulating the growth orientation of palatal shelves. In addition, we observe a unique cleft palate phenotype at the anterior end of the secondary palate, which is likely caused by the severely underdeveloped primary palate in these mutants. Last but not least, we also discover that both Wnt and Shh signalings are essential for tongue development. We provide genetic evidence that disruption of either signaling pathway results in severe microglossia. Altogether, we demonstrate a dynamic role for Wnt-β-Catenin signaling in the development of the oral apparatus.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Lin C,Fisher AV,Yin Y,Maruyama T,Veith GM,Dhandha M,Huang GJ,Hsu W,Ma Ldoi
10.1016/j.ydbio.2011.05.002subject
Has Abstractpub_date
2011-08-01 00:00:00pages
40-50issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(11)00299-5journal_volume
356pub_type
杂志文章abstract：:MHM is a chicken Z chromosome-linked locus that is methylated and transcriptionally silent in male cells, but is hypomethylated and transcribed into a long non-coding RNA in female cells. MHM has been implicated in both localised dosage compensation and sex determination in the chicken embryo, but direct evidence is l...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.03.025
更新日期：2012-06-15 00:00:00
abstract：:We have identified cDNA clones encoding a testis-specific poly(A) polymerase, termed TPAP, a candidate molecule responsible for cytoplasmic polyadenylation of preexisting mRNAs in male haploid germ cells. The TPAP gene was most abundantly expressed coincident with the additional elongation of mRNA poly(A) tails in rou...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9894
更新日期：2000-12-01 00:00:00
abstract：:Primary cultures of cardiac myocytes from newborn normal and genetically cardiomyopathic (strain UM-X7.1) hamsters were analyzed by electron microscopy and immunofluorescent staining for myosin, actin, tropomyosin, and alpha-actinin. Antibody staining of these contractile proteins demonstrates that both normal and car...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90091-x
更新日期：1983-06-01 00:00:00
abstract：:We have investigated sensillum development in Drosophila embryos homozygous for mutations in the locus string (stg). In these embryos, cell division is blocked following blastoderm formation. This permits a study of the differentiative fate of undivided precursor cells, in particular those giving rise to the larval se...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90184-k
更新日期：1990-03-01 00:00:00
abstract：:Retinoic acid (RA) plays a critical role in neural patterning and organogenesis in the vertebrate embryo. Here we characterize a mutant of the zebrafish named giraffe (gir) in which the gene for the RA-degrading enzyme Cyp26a1 is mutated. The gir mutant displayed patterning defects in multiple organs including the com...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.11.023
更新日期：2005-02-15 00:00:00
abstract：:Using sea urchin embryos, we demonstrate that the MEK/MAPK/ERK cascade is essential for the proper progression of the cell cycle. Activation of a limited fraction of MAPK/ERK is required between S-phase and M-phase. Neither DNA replication nor CDK1 activation are impacted by the inhibition of this small active MAPK/ER...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.11.018
更新日期：2017-01-15 00:00:00
abstract：:The regulation of cell cycle rate is essential for the correct timing of proliferation and differentiation during development. Changes to cell cycle rate can have profound effects on the size, shape and cell types of a developing organ. We previously identified a zebrafish mutant ceylon (cey) that has a severe reducti...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.05.024
更新日期：2012-08-15 00:00:00
abstract：:Several genomic clones were isolated from a Drosophila library screened with cDNA prepared from abundant adult female mRNA. Cytoplasmic dot hybridizations have shown that the genes in all of these clones are expressed only in posteclosion (stages 8-14) follicles. One set of overlapping clones (lambda 20, lambda 28, an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90271-9
更新日期：1984-09-01 00:00:00
abstract：:We have isolated a cDNA of the DEAD protein family from a Xenopus ovary cDNA library. Northern hybridization revealed that the mRNA corresponding to the cDNA [XVLG1 (Xenopus vasa-like gene)] was specifically expressed in adult testis and ovary. The deduced amino acid sequence of XVLG1 was relatively homologous to that...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1093
更新日期：1994-04-01 00:00:00
abstract：:The development of the reproductive system in Caenorhabditis elegans is a well-established model system for patterning and organogenesis. We report the characterization of the cog-1 gene, mutations in which cause novel phenotypes in late patterning in vulval lineages, establishment of the vulva-uterine connection, dev...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0850
更新日期：2002-12-15 00:00:00
abstract：:Spermatogonial stem cells are required for the initiation of spermatogenesis and the continuous production of sperm. In addition, they can acquire pluripotency and differentiate into derivatives of the three embryonic germ layers when cultured in the appropriate conditions. Therefore, understanding the signaling pathw...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.12.013
更新日期：2007-04-01 00:00:00
abstract：:The Drosophila homeobox gene tinman and its vertebrate homologs Nkx-2.5 and Nkx-2.3 are critical determinants of cardiac development. We report here the identification of a new tinman-related gene, nkx2.7, as well as orthologs of Nkx-2.5 and Nkx-2.3 in the zebrafish. Analysis of their expression in the developing zebr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0341
更新日期：1996-12-15 00:00:00
abstract：:Chick sensory neurons grow to their correct targets in the hindlimb from the outset during normal development and following various experimental manipulations. This may result not because sensory neurons respond to specific limb-derived cues, but because they interact in some way with motoneurons which are responsive ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90023-0
更新日期：1986-12-01 00:00:00
abstract：:During the last stages of fetal life, the immature epithelial cells of the rat lung alveolus develop the properties of mature type 2 cells. Adult type 2 cells rapidly lose these same properties when isolated and maintained in cell culture. We have examined the synthesis of cytokeratin proteins by adult type 2 cells as...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90396-x
更新日期：1988-10-01 00:00:00
abstract：:Activin is a potent inducer of axial mesoderm in vitro and may have a similar role in vivo. Xenopus laevis eggs contain significant amounts of activin or activin-like factors, but maternal activin transcripts have not been detected in Xenopus eggs. The maternal activin protein might be translated from activin beta A o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1265
更新日期：1993-10-01 00:00:00
abstract：:In Hydra long-range interactions between head, bud, and foot formation take place in addition to short-range interactions which are recognized as induction phenomena, occurring when transplants are placed into an environment of disparate positional value. Here the long-range promotion of foot formation is analyzed. Th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1015
更新日期：1995-01-01 00:00:00
abstract：:In vertebrates, hematopoietic and vascular progenitors develop from ventral mesoderm. The first primitive wave of hematopoiesis yields embryonic red blood cells, whereas progenitor cells of subsequent definitive waves form all hematopoietic cell lineages. In this report we examine the development of hematopoietic and ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8887
更新日期：1998-05-15 00:00:00
abstract：:Alkaline phosphatase (AP) activity is expressed in the endodermal cell lineage of ascidian embryos beginning at gastrulation. AP expression is resistant to levels of actinomycin D which completely suppress the appearance of other tissue-specific enzyme and morphological markers including acetylcholinesterase (AchE), a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90043-1
更新日期：1987-02-01 00:00:00
abstract：:The mammalian kidney consists of an array of tubules connected to a ductal system that collectively function to control water/salt balance and to remove waste from the organisms' circulatory system. During mammalian embryogenesis, three kidney structures form within the intermediate mesoderm. The two most anterior str...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.03.044
更新日期：2008-07-15 00:00:00
abstract：:Accumulating evidence indicates that the endometrial extracellular matrix (ECM) modulates trophoblast adhesion during mouse blastocyst implantation. In previous studies of adhesion-competent mouse blastocysts, we have demonstrated that integrin-mediated fibronectin (FN)-binding activity on the apical surface of tropho...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0644
更新日期：2002-05-15 00:00:00
abstract：:Spore germination is a defined developmental process that marks a critical point in the life cycle of Dictyostelium discoideum. Upon germination the environmental conditions must be conducive to cell growth to ensure survival of emerged amoebae. However, the signal transduction pathways controlling the various aspects...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8854
更新日期：1998-04-15 00:00:00
abstract：:The notochord is the defining characteristic of the chordate embryo and plays critical roles as a signaling center and as the primitive skeleton. In this study we show that early notochord development in Xenopus embryos is regulated by apoptosis. We find apoptotic cells in the notochord beginning at the neural groove ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.08.047
更新日期：2007-11-15 00:00:00
abstract：:Homeotic genes in the Antennapedia Complex of Drosophila specify identity of the posterior head segments; the labial segment requires Sex combs reduced (Scr) for proper development, Deformed (Dfd) specifies maxillary and mandibular identity, and labial is necessary for intercalary segment identity. Although mutations ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0290
更新日期：1996-11-25 00:00:00
abstract：:The Drosophila salivary gland is a simple tubular organ derived from a contiguous epithelial primordium, which is established by the activities of the homeodomain-containing proteins Sex combs reduced (SCR), Extradenticle (EXD), and Homothorax (HTH). EGF signaling along the ventral midline specifies the salivary duct ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00140-4
更新日期：2003-06-15 00:00:00
abstract：:Hox genes encode transcription factors which provide positional information during morphogenesis along the body axis; genetic interaction among Hox genes is thought to be necessary for correct pattern formation. One of the most curious features of the 39 vertebrate Hox genes is that they form four clusters each compos...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9651
更新日期：2000-04-15 00:00:00
abstract：:The outstanding migration and differentiation capacities of neural crest cells (NCCs) have fascinated scientists since Wilhelm His described this cell population in 1868. Today, after intense research using vertebrate model organisms, we have gained considerable knowledge regarding the origin, migration and differenti...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.05.012
更新日期：2010-08-15 00:00:00
abstract：:Alveologenesis is the final step of lung maturation, which subdivides the alveolar region of the lung into smaller units called alveoli. Each of the nascent dividers serves as a new gas-exchange surface, and collectively they drastically increase the surface area for breathing. Disruption of alveologenesis results in ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.11.017
更新日期：2016-01-15 00:00:00
abstract：:Tubes with distinct shapes and sizes are critical for the proper function of many tubular organs. Here we describe a unique phenotype caused by the loss of a novel, evolutionarily-conserved, Drosophila Smad-like protein, Expansion. In expansion mutants, unicellular and intracellular tracheal branches develop bubble-li...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.06.016
更新日期：2014-09-01 00:00:00
abstract：:The Mesp bHLH genes play a conserved role during segmental patterning of the mesoderm in the vertebrate embryo by specifying segmental boundaries and anteroposterior (A-P) segmental polarity. Here we use a xenotransgenic approach to compare the transcriptional enhancers that drive expression of the Mesp genes within s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.12.038
更新日期：2008-03-15 00:00:00
abstract：:Paracrine signaling mediated by FGF-10 and the FGF-R2IIIb receptor is required for formation of the lung. To determine the temporal requirements for FGF signaling during pulmonary morphogenesis, Sprouty-4 (Spry-4), an intracellular FGF receptor antagonist, was expressed in epithelial cells of the fetal lung under cont...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00106-4
更新日期：2003-06-01 00:00:00
