The pattern of cytokeratin synthesis is a marker of type 2 cell differentiation in adult and maturing fetal lung alveolar cells.


:During the last stages of fetal life, the immature epithelial cells of the rat lung alveolus develop the properties of mature type 2 cells. Adult type 2 cells rapidly lose these same properties when isolated and maintained in cell culture. We have examined the synthesis of cytokeratin proteins by adult type 2 cells as they lose their differentiated characteristics during 1 week in culture, and of immature fetal alveolar epithelial cells as they differentiate either in utero or when cultured on an extracellular matrix. Freshly isolated adult type 2 cells synthesize four cytokeratins which by electrophoretic mobilities and Western blot analysis correspond to human cytokeratins Nos. 7, 8, 18, and 19. During 7 days in culture synthesis of cytokeratin No. 19 is dramatically decreased and cytokeratin No. 18 becomes the predominant acidic cytokeratin produced. Fetal lung epithelial cells at 18 days gestation lack most characteristics of mature type 2 cells. When freshly isolated, these cells synthesize cytokeratins Nos. 7, 8, and 18 but make only minimal amounts of cytokeratin No. 19. When these cells are allowed to mature either in utero or in culture on a whole basement membrane extract, they develop both the morphological characteristics and the pattern of cytokeratin synthesis of fully developed type 2 cells, with cytokeratins No. 19 being the major acidic cytokeratin produced.


Dev Biol


Developmental biology


Paine R,Ben-Ze'ev A,Farmer SR,Brody JS




Has Abstract


1988-10-01 00:00:00














  • Cubitus interruptus acts to specify naked cuticle in the trunk of Drosophila embryos.

    abstract::One function of the Wingless signaling pathway is to determine the naked, cuticle cell fate choice in the trunk epidermis of Drosophila larvae. The zinc finger protein Teashirt binds to the transactivator domain of Armadillo to modulate Wingless signaling output in the embryonic trunk and contributes to the naked cell...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Angelats C,Gallet A,Thérond P,Fasano L,Kerridge S

    更新日期:2002-01-01 00:00:00

  • Alternative splicing of the Endo16 transcript produces differentially expressed mRNAs during sea urchin gastrulation.

    abstract::The Endo16 gene codes for an RGD-containing calcium-binding protein that is found on the basal surfaces and in the extracellular matrix of cells of the invaginating archenteron during sea urchin gastrulation. Previously, we have shown that Endo16 is a single copy gene and we have determined the coding sequence and ana...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Godin RE,Urry LA,Ernst SG

    更新日期:1996-10-10 00:00:00

  • O-fucosylation of thrombospondin type 1 repeats restricts epithelial to mesenchymal transition (EMT) and maintains epiblast pluripotency during mouse gastrulation.

    abstract::Thrombospondin type 1 repeat (TSR) superfamily members regulate diverse biological activities ranging from cell motility to inhibition of angiogenesis. In this study, we verified that mouse protein O-fucosyltransferase-2 (POFUT2) specifically adds O-fucose to TSRs. Using two Pofut2 gene-trap lines, we demonstrated tha...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Du J,Takeuchi H,Leonhard-Melief C,Shroyer KR,Dlugosz M,Haltiwanger RS,Holdener BC

    更新日期:2010-10-01 00:00:00

  • Microinjection of lectins, hyaluronidase, and hyaluronate fragments interferes with cleavage delay and mesoderm induction in embryos of Patella vulgata.

    abstract::In embryos of Patella vulgata at the 32-cell stage, one of the four vegetally located macromeres makes contacts with overlying animal micromeres. As a result, this macromere (designated 3D) divides significantly later than the other macromeres and forms the mesodermal stem cell 4d. Shortly before and during this inter...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kühtreiber WM,van Dongen CA

    更新日期:1989-04-01 00:00:00

  • C. elegans peb-1 mutants exhibit pleiotropic defects in molting, feeding, and morphology.

    abstract::Caenorhabditis elegans PEB-1 is a novel DNA-binding protein expressed in most pharyngeal cell types and outside the pharynx in the hypodermis, hindgut, and vulva. Previous RNAi analyses indicated that PEB-1 is required for normal morphology of these tissues and growth; however, the peb-1 null phenotype was unknown. He...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Fernandez AP,Gibbons J,Okkema PG

    更新日期:2004-12-15 00:00:00

  • Distinct effects of ectopic expression of Wnt-1, activin B, and bFGF on gap junctional permeability in 32-cell Xenopus embryos.

    abstract::A polarity in gap junctional permeability normally exists in 32-cell stage Xenopus embryos, in that dorsal cells are relatively more coupled than ventral cells, as measured by transfer of Lucifer yellow dye. The current study extends our analysis of whether gap junctional permeability at this stage can be modulated by...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Olson DJ,Moon RT

    更新日期:1992-05-01 00:00:00

  • Dynamic localization and functional implications of Aurora-C kinase during male mouse meiosis.

    abstract::Aurora-C was first identified during screening for kinases expressed in mouse sperm and eggs. Herein, we report for the first time the precise subcellular localization of endogenous Aurora-C during male meiotic division. The localization of Aurora-C was analyzed by immunofluorescence staining on chromosome spreads of ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Tang CJ,Lin CY,Tang TK

    更新日期:2006-02-15 00:00:00

  • Misexpression of nautilus induces myogenesis in cardioblasts and alters the pattern of somatic muscle fibers.

    abstract::nautilus (nau), one member of the myogenic regulatory family of bHLH-encoding genes, is expressed in a subset of muscle precursors and differentiated fibers in the Drosophila embryo. To elucidate the role of nautilus in myogenesis, we have misexpressed it using the GAL4-targeted system. We find that ectopic expression...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Keller CA,Erickson MS,Abmayr SM

    更新日期:1997-01-15 00:00:00

  • Early amphibian (anuran) morphogenesis is sensitive to novel gravitational fields.

    abstract::Anuran amphibian embryos (Xenopus laevis and Rana dybowskii) are sensitive to novel gravitational fields. Under simulated weightlessness, (i) the location of the first horizontal cleavage furrow was shifted toward the vegetal pole at the eight-cell stage; (ii) the position of the blastocoel was more centered, and the ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Neff AW,Yokota H,Chung HM,Wakahara M,Malacinski GM

    更新日期:1993-01-01 00:00:00

  • A dual epimorphic and compensatory mode of heart regeneration in zebrafish.

    abstract::Zebrafish heart regeneration relies on the capacity of cardiomyocytes to proliferate upon injury. To understand the principles of this process after cryoinjury-induced myocardial infarction, we established a spatio-temporal map of mitotic cardiomyocytes and their differentiation dynamics. Immunodetection of phosphohis...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Sallin P,de Preux Charles AS,Duruz V,Pfefferli C,Jaźwińska A

    更新日期:2015-03-01 00:00:00

  • Prorenin receptor is critical for nephron progenitors.

    abstract::Deficient nephrogenesis is the major factor contributing to renal hypoplasia defined as abnormally small kidneys. Nephron induction during kidney development is driven by reciprocal interactions between progenitor cells of the cap mesenchyme (CM) and the ureteric bud (UB). The prorenin receptor (PRR) is a receptor for...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Song R,Preston G,Kidd L,Bushnell D,Sims-Lucas S,Bates CM,Yosypiv IV

    更新日期:2016-01-15 00:00:00

  • Heat shock factor 2-like activity in mouse blastocysts.

    abstract::The expression of heat shock genes is induced in all living cells by a series of proteotoxic treatments. Heat shock genes are also activated spontaneously during different phases of embryonic development. HSP89 alpha and HSC70 are expressed at a high level in the mouse blastocyst. A family of factors, called HSFs, are...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Mezger V,Rallu M,Morimoto RI,Morange M,Renard JP

    更新日期:1994-12-01 00:00:00

  • Reexamination of the sequence of the sea urchin egg receptor for sperm: implications with respect to its properties.

    abstract::A reexamination of the cDNA clones of the Strongylocentrotus purpuratus sea urchin egg receptor for sperm resulted in several important changes to the sequence. By using both rapid amplification of cDNA ends (RACE) and Northern blot analysis for confirmation, the corrected deduced amino acid sequence was shown to lack...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Just ML,Lennarz WJ

    更新日期:1997-04-01 00:00:00

  • Protein tyrosine kinase activity following fertilization is required to complete gastrulation, but not for initial differentiation of endoderm and mesoderm in the sea urchin embryo.

    abstract::The egg activation process functions to implement developmental programs that act much later in embryogenesis. One example of this is the fact that application of protein tyrosine kinase inhibitors to the fertilized sea urchin egg for a 15-min period results in a defect in the gastrulation process occurring over 24 h ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Livingston BT,VanWinkle CE,Kinsey WH

    更新日期:1998-01-01 00:00:00

  • Molecular mechanism of mammary gland involution: An update.

    abstract::The mammary gland (MG) is a unique organ responsible for milk synthesis, secretion, and involution to prepare the gland for subsequent lactation. The mammary epithelial cells (MECs), which are the milk synthesizing units of the MG, proliferate, differentiate, undergo apoptosis and regenerate following a cyclic pathway...

    journal_title:Developmental biology

    pub_type: 杂志文章,评审


    authors: Jena MK,Jaswal S,Kumar S,Mohanty AK

    更新日期:2019-01-15 00:00:00

  • Patterns of maternal messenger RNA accumulation and adenylation during oogenesis in Urechis caupo.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Rosenthal ET,Wilt FH

    更新日期:1986-09-01 00:00:00

  • Zebrafish cypher is important for somite formation and heart development.

    abstract::Mammalian CYPHER (Oracle, KIA0613), a member of the PDZ-LIM family of proteins (Enigma/LMP-1, ENH, ZASP/Cypher, RIL, ALP, and CLP-36), has been associated with cardiac and muscular myopathies. Targeted deletion of Cypher in mice is neonatal lethal possibly caused by myopathies. To further investigate the role of cyphe...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: van der Meer DL,Marques IJ,Leito JT,Besser J,Bakkers J,Schoonheere E,Bagowski CP

    更新日期:2006-11-15 00:00:00

  • Multiple roles of the eyes absent gene in Drosophila.

    abstract::The eyes absent (eya) gene plays an essential role in the events that lead to formation of the Drosophila eye; without expression of eya in retinal progenitor cells, they undergo programmed cell death just prior to the morphogenetic furrow, leading to an eyeless or reduced eye phenotype. The eya gene has recently been...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Bonini NM,Leiserson WM,Benzer S

    更新日期:1998-04-01 00:00:00

  • Single-cell analysis of regulatory gene expression in quiescent and activated mouse skeletal muscle satellite cells.

    abstract::Repair and regeneration of adult skeletal muscle are mediated by satellite cells. In healthy muscle these rare mononucleate muscle precursor cells are mitotically quiescent. Upon muscle injury or degeneration, members of this self-renewing pool are activated to proliferate and then differentiate. Here we analyzed in s...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Cornelison DD,Wold BJ

    更新日期:1997-11-15 00:00:00

  • Analyses of fugu hoxa2 genes provide evidence for subfunctionalization of neural crest cell and rhombomere cis-regulatory modules during vertebrate evolution.

    abstract::Hoxa2 gene is a primary player in regulation of craniofacial programs of head development in vertebrates. Here we investigate the evolution of a Hoxa2 neural crest enhancer identified originally in mouse by comparing and contrasting the fugu hoxa2a and hoxa2b genes with their orthologous teleost and mammalian sequence...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: McEllin JA,Alexander TB,Tümpel S,Wiedemann LM,Krumlauf R

    更新日期:2016-01-15 00:00:00

  • The exon junction complex is required for stem and progenitor cell maintenance in planarians.

    abstract::Named for its assembly near exon-exon junctions during pre-mRNA splicing, the exon junction complex (EJC) regulates multiple aspects of RNA biochemistry, including export of spliced mRNAs from the nucleus and translation. Transcriptome analyses have revealed broad EJC occupancy of spliced metazoan transcripts, yet inh...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kimball C,Powers K,Dustin J,Poirier V,Pellettieri J

    更新日期:2020-01-01 00:00:00

  • CLIP-50 immunolocalization during mouse spermiogenesis suggests a role in shaping the sperm nucleus.

    abstract::The spermatid nucleus and cytoplasm undergo dramatic morphological modifications during spermatid differentiation into mature sperm. Some of the external force causing this nuclear shaping is generated by a microtubular structure termed the manchette, which attaches to the perinuclear ring of the spermatid. Here, we r...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Tarsounas M,Pearlman RE,Moens PB

    更新日期:2001-08-15 00:00:00

  • Characterization of an islet-activating protein-sensitive site in mouse sperm that is involved in the zona pellucida-induced acrosome reaction.

    abstract::Recently, it has been suggested that a mouse sperm-associated protein with properties similar to the inhibitory guanine nucleotide-binding regulatory protein Gi plays an intermediary role in the zona pellucida-induced acrosome reaction (Endo, Y., Lee, M. A., and Kopf, G. S. 1987. Dev. Biol. 119, 210-216). In the prese...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Endo Y,Lee MA,Kopf GS

    更新日期:1988-09-01 00:00:00

  • Dorsoventral patterning by the Chordin-BMP pathway: a unified model from a pattern-formation perspective for Drosophila, vertebrates, sea urchins and Nematostella.

    abstract::Conserved from Cnidarians to vertebrates, the dorsoventral (DV) axis is patterned by the Chordin-BMP pathway. However, the functions of the pathway's components are very different in different phyla. By modeling it is shown that many observations can be integrated by the assumption that BMP, acting as an inhibitory co...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Meinhardt H

    更新日期:2015-09-01 00:00:00

  • Structural analysis of three subunits of laminin from teratocarcinoma-derived parietal endoderm cells.

    abstract::The structure of the three polypeptide chains of the laminin subunits and the number of glycosylation sites in each polypeptide chain were determined using peptide mapping by high-performance liquid chromatography. Analysis of the [35S]methionine-labeled underglycosylated laminin isolated from tunicamycin (TM)-treated...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Howe CC,Dietzschold B

    更新日期:1983-08-01 00:00:00

  • Binding of both acrosome-intact and acrosome-reacted guinea pig sperm to the zona pellucida during in vitro fertilization.

    abstract::Mammalian sperm-egg adhesion occurs when sperm bind to the zona pellucida of the egg. In this study with guinea pig gametes, we have asked if sperm can initiate binding to the zona before and after the acrosome reaction and if the sperm surface protein PH-20 is involved in the binding at these two stages. Sperm bindin...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Myles DG,Hyatt H,Primakoff P

    更新日期:1987-06-01 00:00:00

  • Glypican-3 modulates BMP- and FGF-mediated effects during renal branching morphogenesis.

    abstract::The kidney of the Gpc3-/ mouse, a novel model of human renal dysplasia, is characterized by selective degeneration of medullary collecting ducts preceded by enhanced cell proliferation and overgrowth during branching morphogenesis. Here, we identify cellular and molecular mechanisms underlying this renal dysplasia. Gl...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Grisaru S,Cano-Gauci D,Tee J,Filmus J,Rosenblum ND

    更新日期:2001-03-01 00:00:00

  • Hox C cluster genes are dispensable for overall body plan of mouse embryonic development.

    abstract::Hox genes encode transcription factors which provide positional information during morphogenesis along the body axis; genetic interaction among Hox genes is thought to be necessary for correct pattern formation. One of the most curious features of the 39 vertebrate Hox genes is that they form four clusters each compos...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Suemori H,Noguchi S

    更新日期:2000-04-15 00:00:00

  • Cell differentiation and morphogenesis in the colony-forming choanoflagellate Salpingoeca rosetta.

    abstract::It has been posited that animal development evolved from pre-existing mechanisms for regulating cell differentiation in the single celled and colonial ancestors of animals. Although the progenitors of animals cannot be studied directly, insights into their cell biology may be gleaned from comparisons between animals a...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Dayel MJ,Alegado RA,Fairclough SR,Levin TC,Nichols SA,McDonald K,King N

    更新日期:2011-09-01 00:00:00

  • Inhibition by dibutyryl cyclic AMP of the transition to metaphase of mouse oocyte nuclei and its reversal by cell fusion to metaphase oocytes.

    abstract::Mouse oocytes at metaphase I of meiotic maturation were treated with puromycin, which caused the condensed chromosomes to become decondensed to form an interphase nucleus. The chromosomes returned to a metaphase state 6.3 hr after the oocytes were transferred to puromycin-free medium [H. J. Clarke and Y. Masui (1983) ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Clarke HJ,Masui Y

    更新日期:1985-03-01 00:00:00