The pattern of cytokeratin synthesis is a marker of type 2 cell differentiation in adult and maturing fetal lung alveolar cells.


:During the last stages of fetal life, the immature epithelial cells of the rat lung alveolus develop the properties of mature type 2 cells. Adult type 2 cells rapidly lose these same properties when isolated and maintained in cell culture. We have examined the synthesis of cytokeratin proteins by adult type 2 cells as they lose their differentiated characteristics during 1 week in culture, and of immature fetal alveolar epithelial cells as they differentiate either in utero or when cultured on an extracellular matrix. Freshly isolated adult type 2 cells synthesize four cytokeratins which by electrophoretic mobilities and Western blot analysis correspond to human cytokeratins Nos. 7, 8, 18, and 19. During 7 days in culture synthesis of cytokeratin No. 19 is dramatically decreased and cytokeratin No. 18 becomes the predominant acidic cytokeratin produced. Fetal lung epithelial cells at 18 days gestation lack most characteristics of mature type 2 cells. When freshly isolated, these cells synthesize cytokeratins Nos. 7, 8, and 18 but make only minimal amounts of cytokeratin No. 19. When these cells are allowed to mature either in utero or in culture on a whole basement membrane extract, they develop both the morphological characteristics and the pattern of cytokeratin synthesis of fully developed type 2 cells, with cytokeratins No. 19 being the major acidic cytokeratin produced.


Dev Biol


Developmental biology


Paine R,Ben-Ze'ev A,Farmer SR,Brody JS




Has Abstract


1988-10-01 00:00:00














  • Activin signals via SMAD2/3 between germ and somatic cells in the human fetal ovary and regulates kit ligand expression.

    abstract::Ovarian germ cell survival is dependent upon the formation of primordial follicles, which occurs during fetal life in the human. Activin contributes to germ cell proliferation and survival at this time. SMADs2 and 3 are central elements in the activin signalling pathway and thus indicate sites of activin action. We ha...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Coutts SM,Childs AJ,Fulton N,Collins C,Bayne RA,McNeilly AS,Anderson RA

    更新日期:2008-02-01 00:00:00

  • prep1.2 and aldh1a2 participate to a positive loop required for branchial arches development in zebrafish.

    abstract::Segmentation is a key step in embryonic development. Acting in all germ layers, it is responsible for the generation of antero-posterior asymmetries. Hox genes, with their diverse expression in individual segments, are fundamental players in the determination of different segmental fates. In vertebrates, Hox gene prod...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Vaccari E,Deflorian G,Bernardi E,Pauls S,Tiso N,Bortolussi M,Argenton F

    更新日期:2010-07-01 00:00:00

  • Protein deiminases: new players in the developmentally regulated loss of neural regenerative ability.

    abstract::Spinal cord regenerative ability is lost with development, but the mechanisms underlying this loss are still poorly understood. In chick embryos, effective regeneration does not occur after E13, when spinal cord injury induces extensive apoptotic response and tissue damage. As initial experiments showed that treatment...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Lange S,Gögel S,Leung KY,Vernay B,Nicholas AP,Causey CP,Thompson PR,Greene ND,Ferretti P

    更新日期:2011-07-15 00:00:00

  • Identification of cis regulatory features in the embryonic zebrafish genome through large-scale profiling of H3K4me1 and H3K4me3 binding sites.

    abstract::An organism's genome sequence serves as a blueprint for the proteins and regulatory RNAs essential for cellular function. The genome also harbors cis-acting non-coding sequences that control gene expression and are essential to coordinate regulatory programs during embryonic development. However, the genome sequence i...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Aday AW,Zhu LJ,Lakshmanan A,Wang J,Lawson ND

    更新日期:2011-09-15 00:00:00

  • The Drosophila E93 gene from the 93F early puff displays stage- and tissue-specific regulation by 20-hydroxyecdysone.

    abstract::Pulses of ecdysteroids induce dramatic changes in gene expression that direct the early stages of Drosophila metamorphosis. This gene activity is reflected by the appearance of early and late puffs in the salivary gland polytene chromosomes. Curiously, the early puff genes that have been studied to date are induced by...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Baehrecke EH,Thummel CS

    更新日期:1995-09-01 00:00:00

  • Locale and level of bindin mRNA in maturing testis of the sea urchin, Strongylocentrotus purpuratus.

    abstract::This first study of the onset of spermatogenesis in the sea urchin, Strongylocentrotus purpuratus, was undertaken using individuals reared in the laboratory. Spermatogenesis commences about 11-12 months after metamorphosis in these animals. Bindin message accumulates in late spermatocytes and early spermatids which li...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Cameron RA,Minor JE,Nishioka D,Britten RJ,Davidson EH

    更新日期:1990-11-01 00:00:00

  • The representation of heart development in the gene ontology.

    abstract::An understanding of heart development is critical in any systems biology approach to cardiovascular disease. The interpretation of data generated from high-throughput technologies (such as microarray and proteomics) is also essential to this approach. However, characterizing the role of genes in the processes underlyi...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Khodiyar VK,Hill DP,Howe D,Berardini TZ,Tweedie S,Talmud PJ,Breckenridge R,Bhattarcharya S,Riley P,Scambler P,Lovering RC

    更新日期:2011-06-01 00:00:00

  • Septin7 regulates inner ear formation at an early developmental stage.

    abstract::Septins are guanosine triphosphate-binding proteins that are evolutionally conserved in all eukaryotes other than plants. They function as multimeric complexes that interact with membrane lipids, actomyosin, and microtubules. Based on these interactions, septins play essential roles in the morphogenesis and physiologi...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Torii H,Yoshida A,Katsuno T,Nakagawa T,Ito J,Omori K,Kinoshita M,Yamamoto N

    更新日期:2016-11-15 00:00:00

  • GATA2 regulates Wnt signaling to promote primitive red blood cell fate.

    abstract::Primitive erythropoiesis is regulated in a non cell-autonomous fashion across evolution from frogs to mammals. In Xenopus laevis, signals from the overlying ectoderm are required to induce the mesoderm to adopt an erythroid fate. Previous studies in our lab identified the transcription factor GATA2 as a key regulator ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Mimoto MS,Kwon S,Green YS,Goldman D,Christian JL

    更新日期:2015-11-01 00:00:00

  • SpRunt-1, a new member of the runt domain family of transcription factors, is a positive regulator of the aboral ectoderm-specific CyIIIA gene in sea urchin embryos.

    abstract::In this paper we present a structural and functional characterization of a new sea urchin embryo transcription factor, SpRunt-1. This factor was isolated by means of its specific interaction with a cis-regulatory target site of the CyIIIa gene. Here we show that this target site, the P7I site, is required for normal e...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Coffman JA,Kirchhamer CV,Harrington MG,Davidson EH

    更新日期:1996-02-25 00:00:00

  • Morphological differentiation of embryonic rat sympathetic neurons in tissue culture. I. Conditions under which neurons form axons but not dendrites.

    abstract::We have examined the morphology of fetal rat sympathetic neurons grown in serum-free medium in the absence of nonneuronal cells. Because cell density can affect phenotypic expression in vitro, the morphological analysis was subdivided into the study of isolated neurons (neurons whose somata were at least 150 micron fr...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Bruckenstein DA,Higgins D

    更新日期:1988-08-01 00:00:00

  • A developmentally regulated wound epithelial antigen of the newt limb regenerate is also present in a variety of secretory/transport cell types.

    abstract::The role of the wound epithelium in amphibian limb regeneration is not understood. We showed previously that monoclonal antibody (mAb) WE3 stains the wound epithelium but not skin epidermis, suggesting that the WE3 antigen may be a marker for, or be important in, the function of the wound epithelium. In the present st...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Goldhamer DJ,Tomlinson BL,Tassava RA

    更新日期:1989-10-01 00:00:00

  • Cell lineage conversion in the sea urchin embryo.

    abstract::The mesoderm of the sea urchin embryo conventionally is divided into two populations of cells; the primary mesenchyme cells (PMCs), which produce the larval skeleton, and the secondary mesenchyme cells (SMCs), which differentiate into a variety of cell types but do not participate in skeletogenesis. In this study we e...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Ettensohn CA,McClay DR

    更新日期:1988-02-01 00:00:00

  • The sperm surface localization of the TRP-3/SPE-41 Ca2+ -permeable channel depends on SPE-38 function in Caenorhabditis elegans.

    abstract::Despite undergoing normal development and acquiring normal morphology and motility, mutations in spe-38 or trp-3/spe-41 cause identical phenotypes in Caenorhabditis elegans-mutant sperm fail to fertilize oocytes despite direct contact. SPE-38 is a novel, four-pass transmembrane protein and TRP-3/SPE-41 is a Ca(2+)-per...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Singaravelu G,Chatterjee I,Rahimi S,Druzhinina MK,Kang L,Xu XZ,Singson A

    更新日期:2012-05-15 00:00:00

  • A divergent Tbx6-related gene and Tbx6 are both required for neural crest and intermediate mesoderm development in Xenopus.

    abstract::T-box family transcription factors play many roles in Metazoan development. Here we characterise Tbx6r, a unique Tbx6 paralogue isolated from the amphibian Xenopus. The evolution and developmental integration of this divergent T-box gene within the vertebrates reveals an unexpected level of plasticity within this cons...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Callery EM,Thomsen GH,Smith JC

    更新日期:2010-04-01 00:00:00

  • Ecdysone regulates morphogenesis and function of Malpighian tubules in Drosophila melanogaster through EcR-B2 isoform.

    abstract::Malpighian tubules are the osmoregulatory and detoxifying organs of Drosophila and its proper development is critical for the survival of the organism. They are made up of two major cell types, the ectodermal principal cells and mesodermal stellate cells. The principal and stellate cells are structurally and physiolog...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Gautam NK,Verma P,Tapadia MG

    更新日期:2015-02-15 00:00:00

  • Formation of nucleus-like structure in the cytoplasm of lambda-DNA-injected fertilized eggs and its partition into blastomeres during early embryogenesis in Xenopus laevis.

    abstract::The fate of bacteriophage lambda-DNA was examined after injection into the fertilized eggs of Xenopus laevis. Injection of a large amount of lambda-DNA (ca. 24 ng) into a fertilized Xenopus egg induced the formation around the injected DNA of a giant nucleus-like structure which was surrounded by an apparently normal ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Shiokawa K,Sameshima M,Tashiro K,Miura T,Nakakura N,Yamana K

    更新日期:1986-08-01 00:00:00

  • The biology of the extracorporeal vasculature of Botryllus schlosseri.

    abstract::The extracorporeal vasculature of the colonial ascidian Botryllus schlosseri plays a key role in several biological processes: transporting blood, angiogenesis, regeneration, self-nonself recognition, and parabiosis. The vasculature also interconnects all individuals in a colony and is composed of a single layer of ec...

    journal_title:Developmental biology

    pub_type: 杂志文章,评审


    authors: Rodriguez D,Nourizadeh S,De Tomaso AW

    更新日期:2019-04-15 00:00:00

  • Regulation of locomotion and motoneuron trajectory selection and targeting by the Drosophila homolog of Olig family transcription factors.

    abstract::During the development of locomotion circuits it is essential that motoneurons with distinct subtype identities select the correct trajectories and target muscles. In vertebrates, the generation of motoneurons and myelinating glia depends on Olig2, one of the five Olig family bHLH transcription factors. We investigate...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Oyallon J,Apitz H,Miguel-Aliaga I,Timofeev K,Ferreira L,Salecker I

    更新日期:2012-09-15 00:00:00

  • Opposing transcriptional and post-transcriptional roles for Scalloped in binary Hippo-dependent neural fate decisions.

    abstract::The Hippo tumor suppressor pathway plays many fundamental cell biological roles during animal development. Two central players in controlling Hippo-dependent gene expression are the TEAD transcription factor Scalloped (Sd) and its transcriptional co-activator Yorkie (Yki). Hippo signaling phosphorylates Yki, thereby b...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Xie B,Morton DB,Cook TA

    更新日期:2019-11-01 00:00:00

  • Coordinated activity of Spry1 and Spry2 is required for normal development of the external genitalia.

    abstract::Development of the mammalian external genitalia is controlled by a network of signaling molecules and transcription factors. Because FGF signaling plays a central role in this complicated morphogenetic process, we investigated the role of Sprouty genes, which are important intracellular modulators of FGF signaling, du...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Ching ST,Cunha GR,Baskin LS,Basson MA,Klein OD

    更新日期:2014-02-01 00:00:00

  • Persistent expression of Hlxb9 in the pancreatic epithelium impairs pancreatic development.

    abstract::The homebox gene Hlxb9, encoding Hb9, exhibits a dual expression profile during pancreatic development. The early expression in the dorsal and ventral pancreatic epithelium is transient and spans from embryonic day (e) 8 to e9-e10, whereas the later expression is confined to differentiating beta-cells as they appear. ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Li H,Edlund H

    更新日期:2001-12-01 00:00:00

  • Derepression of sonic hedgehog signaling upon Gpr161 deletion unravels forebrain and ventricular abnormalities.

    abstract::Inverse gradients of transcriptional repressors antagonize the transcriptional effector response to morphogens. However, the role of such inverse regulation might not manifest solely from lack of repressors. Sonic hedgehog (Shh) patterns the forebrain by being expressed ventrally; however, absence of antagonizing Gli3...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Shimada IS,Somatilaka BN,Hwang SH,Anderson AG,Shelton JM,Rajaram V,Konopka G,Mukhopadhyay S

    更新日期:2019-06-01 00:00:00

  • Developmental patterning in the Caenorhabditis elegans hindgut.

    abstract::Developmental pattern formation allows cells within a tissue or organ to coordinate their development and establish cell types in relationship to one another. To better characterize the developmental patterning events within one organ, the C. elegans hindgut, we have analyzed the expression pattern of several genes us...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Sewell ST,Zhang G,Uttam A,Chamberlin HM

    更新日期:2003-10-01 00:00:00

  • Evolutionary developmental biology its roots and characteristics.

    abstract::The rise of evolutionary developmental biology was not the progressive isolation and characterization of developmental genes and gene networks. Many obstacles had to be overcome: the idea that all genes were more or less involved in development; the evidence that developmental processes in insects had nothing in commo...

    journal_title:Developmental biology

    pub_type: 历史文章,杂志文章


    authors: Morange M

    更新日期:2011-09-01 00:00:00

  • Direct flight muscles in Drosophila develop from cells with characteristics of founders and depend on DWnt-2 for their correct patterning.

    abstract::The direct flight muscles (DFMs) of Drosophila allow for the fine control of wing position necessary for flight. In DWnt-2 mutant flies, certain DFMs are either missing or fail to attach to the correct epithelial sites. Using a temperature-sensitive allele, we show that DWnt-2 activity is required only during pupation...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kozopas KM,Nusse R

    更新日期:2002-03-15 00:00:00

  • The MEK inhibitor, U0126, alters fertilization-induced [Ca2+]i oscillation parameters and secretion: differential effects associated with in vivo and in vitro meiotic maturation.

    abstract::Although mitogen-activated protein kinase (MAPK) is a well-known cell cycle regulator, emerging studies have also implicated its activity in the regulation of intracellular calcium concentration ([Ca2+](i)) and secretion. Those studies raise the hypothesis that MAPK activity during oocyte maturation and early fertiliz...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Matson S,Ducibella T

    更新日期:2007-06-15 00:00:00

  • The 3'UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin.

    abstract::Nanos is a translational regulator required for the survival and maintenance of primordial germ cells during embryogenesis. Three nanos homologs are present in the genome of the sea urchin Strongylocentrotus purpuratus (Sp), and each nanos mRNA accumulates specifically in the small micromere (sMic) lineage. We found t...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Oulhen N,Yoshida T,Yajima M,Song JL,Sakuma T,Sakamoto N,Yamamoto T,Wessel GM

    更新日期:2013-05-01 00:00:00

  • Activation of Gbetagamma signaling downstream of Wnt-11/Xfz7 regulates Cdc42 activity during Xenopus gastrulation.

    abstract::Wnt-11/Xfz7 signaling plays a major role in the regulation of convergent extension movements affecting the dorsal marginal zone (DMZ) of gastrulating Xenopus embryos. In order to provide data concerning the molecular targets of Wnt-11/Xfz7 signals, we have analyzed the regulation of the Rho GTPase Cdc42 by Wnt-11. In ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Penzo-Mendèz A,Umbhauer M,Djiane A,Boucaut JC,Riou JF

    更新日期:2003-05-15 00:00:00

  • TGF-beta type I receptor Alk5 regulates tooth initiation and mandible patterning in a type II receptor-independent manner.

    abstract::TGF-beta superfamily members signal through a heteromeric receptor complex to regulate craniofacial development. TGF-beta type II receptor appears to bind only TGF-beta, whereas TGF-beta type I receptor (ALK5) also binds to ligands in addition to TGF-beta. Our previous work has shown that conditional inactivation of T...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Zhao H,Oka K,Bringas P,Kaartinen V,Chai Y

    更新日期:2008-08-01 00:00:00