Abstract：
:Previous work in our laboratory has shown that a 62- to 64-kDa protein was a major component of the perinuclear ring of manchettes fractionated from rat spermatids. Mass spectrometry analysis of this protein indicated the presence of a glycine-rich domain homologous to human keratin 9 (K9). Several antibodies to K9, raised against synthetic peptides of human K9, recognized the 64- to 62-kDa protein in the perinuclear ring of the manchette as well as in keratinocytes of the suprabasal layer of the rat and human footpad/sole epidermis in both immunoblotting and immunocytochemical experiments. Based on these data, human-derived K9 primers were used to clone rat K9 cDNA from epidermis by RT-PCR. Rat-specific K9 primers were then used to perform a two-step (nested) PCR to amplify the K9-specific rat testicular RNA and to obtain cDNA to demonstrate K9 gene expression in rat testis. The deduced amino acid sequence of rat K9 cDNA contains 618 amino acids with an estimated molecular mass of 63,020 Da, in agreement with that obtained by electrophoretic fractionation of rat manchette and epidermis footpad proteins. The deduced protein structure correlates with the recognizable pattern of keratins: a rod domain of 304 amino acids with well-conserved initiation and termination sequences (MQNLNSRLASY and EIETYRKLLEG, respectively), flanked by glycine/serine-rich head and tail domains of 141 and 173 amino acids, respectively. A high content of phenylalanine was detected in the head domain and a repetitive motif (SGGSYGGGS) in the tail domain. A comparison with human keratin 9 showed an overall nucleotide and amino acid similarity of 75%. An increased level of K9 transcripts was detected in a cDNA library prepared from fractionated round spermatids. Results of this study show that rat testis expresses K9 and that this protein is a component the perinuclear ring of the manchette of rat spermatids.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Mochida K,Rivkin E,Gil M,Kierszenbaum ALdoi
10.1006/dbio.2000.9911subject
Has Abstractpub_date
2000-11-15 00:00:00pages
510-9issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(00)99911-1journal_volume
227pub_type
杂志文章abstract：:Chick sensory neurons grow to their correct targets in the hindlimb from the outset during normal development and following various experimental manipulations. This may result not because sensory neurons respond to specific limb-derived cues, but because they interact in some way with motoneurons which are responsive ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90023-0
更新日期：1986-12-01 00:00:00
abstract：:The molecular and cellular mechanism for clearance of dead neurons was explored in the developing Drosophila optic lobe. During development of the optic lobe, many neural cells die through apoptosis, and corpses are immediately removed in the early pupal stage. Most of the cells that die in the optic lobe are young ne...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.05.003
更新日期：2019-09-01 00:00:00
abstract：:The morphology of skeletal tissues formed in each of the branchial arches of higher vertebrates is unique. In addition to these structures, which are derived from the neural crest, the crest-derived connective tissues and mesodermal muscles also form different patterns in each of the branchial arches. The objective of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90318-4
更新日期：1983-03-01 00:00:00
abstract：:COUP-TFII (also known as Nr2f2), a member of the nuclear orphan receptor superfamily, is expressed in several regions of the central nervous system (CNS), including the ventral thalamus, hypothalamus, midbrain, pons, and spinal cord. To address the function of COUP-TFII in the CNS, we generated conditional COUP-TFII k...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.11.001
更新日期：2009-02-15 00:00:00
abstract：:A pair of the Drosophila eye-antennal disc gives rise to four distinct organs (eyes, antennae, maxillary palps, and ocelli) and surrounding head cuticle. Developmental processes of this imaginal disc provide an excellent model system to study the mechanism of regional specification and subsequent organogenesis. The do...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.06.015
更新日期：2011-08-15 00:00:00
abstract：:The interaction of heterologous tissues involves cell adhesion mediated by the extracellular matrix and its receptor integrins. The Drosophila wing disc is an ectodermal invagination that contacts specific tracheal branches at the basolateral cell surface. We show that an alpha subunit of laminin, encoded by wing blis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.12.022
更新日期：2007-04-01 00:00:00
abstract：:The first step in the development of the Drosophila optic medullar primordia is the expansion of symmetrically dividing neuroepithelial cells (NEs); this step is then followed by the appearance of asymmetrically dividing neuroblasts (NBs). However, the mechanisms responsible for the change from NEs to NBs remain uncle...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.12.044
更新日期：2011-03-01 00:00:00
abstract：:A modification of the scrape-loading/dye transfer technique was used to study gap junctional communication along the anterior-posterior (A-P) axis of embryonic chick wing buds at an early stage of development (stage 20/21) when positional values along the A-P axis are being specified. Extensive intercellular transfer ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90271-4
更新日期：1991-12-01 00:00:00
abstract：:Breaking symmetry in populations of uniform cells, to induce adoption of an alternative cell fate, is an essential developmental mechanism. Similarly, domain and boundary establishment are crucial steps to forming organs during development. Notch signaling is a pathway ideally suited to mediating precise patterning cu...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2017.09.032
更新日期：2019-03-01 00:00:00
abstract：:The evolution of canalized traits is a central question in evolutionary biology. Natural variation in highly conserved traits can provide clues about their evolutionary potential. Here we investigate natural variation in a conserved trait-even-skipped (eve) expression at the cellular blastoderm stage of embryonic deve...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.06.019
更新日期：2015-09-01 00:00:00
abstract：:Repulsion plays a fundamental role in the establishment of a topographic map of the chick retinotectal projections. This has been highlighted by studies demonstrating the role of opposing gradients of the EphA3 receptor tyrosine kinase on retinal axons and two of its ligands, ephrin-A2 and ephrin-A5, in the tectum. We...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0268
更新日期：2001-06-15 00:00:00
abstract：:How natural variation in embryo size affects patterning of the Drosophila embryo dorsal-ventral (DV) axis is not known. Here we examined quantitatively the relationship between nuclear distribution of the Dorsal transcription factor, boundary positions for several target genes, and DV axis length. Data were obtained f...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.06.020
更新日期：2013-09-01 00:00:00
abstract：:NK-2-class homeobox genes have been identified in a variety of metazoans, from sponges to arthropods and vertebrates, and have been shown to play roles in a variety of cell and tissue specifications. Here we describe the characterization of the NK-2 homolog CnNK-2 from Hydra vulgaris, a freshwater cnidarian. CnNK-2 ex...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0321
更新日期：1996-12-15 00:00:00
abstract：:Murine ovarian folliculogenesis commences after birth involving oocyte growth, somatic cell differentiation and structural remodeling of follicle stromal boundaries. The extracellular metalloproteinase ADAMTS-1 has activity against proteoglycans and collagen and is produced by the granulosa cells of ovarian follicles....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.10.012
更新日期：2006-12-15 00:00:00
abstract：:In the small-optic-lobes (sol) and sine oculis (so) mutants of Drosophila melanogaster extensive cell death occurs in the optic lobes during the first half of pupal development. Gynandromorph flies show that the sol mutation acts primarily on cells of the medulla cortex. Degeneration of medullar ganglion cells occurs ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90050-2
更新日期：1984-07-01 00:00:00
abstract：:Satellite cells, also known as muscle stem cells, are responsible for skeletal muscle growth and repair in mammals. Pax7 and Pax3 transcription factors are established satellite cell markers required for muscle development and regeneration, and there is great interest in identifying additional factors that regulate sa...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.03.004
更新日期：2017-04-15 00:00:00
abstract：:In adult vertebrates, fibroblast growth factor (FGF) synergizes with many hematopoietic cytokines to stimulate the proliferation of hematopoietic progenitors. In vertebrate development, the FGF signaling pathway is important in the formation of some derivatives of ventroposterior mesoderm. However, the function of FGF...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9205
更新日期：1999-04-15 00:00:00
abstract：:T-box family transcription factors play many roles in Metazoan development. Here we characterise Tbx6r, a unique Tbx6 paralogue isolated from the amphibian Xenopus. The evolution and developmental integration of this divergent T-box gene within the vertebrates reveals an unexpected level of plasticity within this cons...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.01.013
更新日期：2010-04-01 00:00:00
abstract：:The current academic and business landscape does not provide a clear path for doctoral trainees graduating. Moreover, the fundamental need of the hour is diversification of the workforce where PhD graduates need to be trained to do more beyond basic scientific research. One of the possible paths that is now becoming p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.10.025
更新日期：2020-03-01 00:00:00
abstract：:The monoclonal antibody HNK-1 was used to identify neural crest cells in serial sections of avian embryos to provide a detailed description of the distribution of trunk neural crest cells. The results indicate the presence of three migratory routes in the trunk: (1) a ventral pathway through the anterior sclerotome; (...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90226-5
更新日期：1986-05-01 00:00:00
abstract：:Cerebral and spinal vascular systems are organized individually, and they then conjugate at their border, through the integration of basilar artery and vertebral arteries. Zebrafish (Danio rerio) is an ideal organism for studying early vascular development, and the precise procedure of cranial and truncal vascular for...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.07.015
更新日期：2015-10-01 00:00:00
abstract：:In studies initially focused on roles of nonmuscle myosin IIA (NMIIA) in the developing mouse epidermis, we have discovered that a previously described cytokeratin 5 (K5)-Cre gene construct is expressed in early embryo development. Mice carrying floxed alleles of the nonmuscle myosin II heavy chain gene (NMHC IIA(flox...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.07.017
更新日期：2013-10-01 00:00:00
abstract：:Embryonic kidney development begins with the outgrowth of the ureteric bud (UB) from the Wolffian duct (WD) into the adjacent metanephric mesenchyme (MM). Both a GDNF-dependent and GDNF-independent (Maeshima et al., 2007) pathway have been identified. In vivo and in vitro, the GDNF-dependent pathway is inhibited by BM...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.08.029
更新日期：2010-11-15 00:00:00
abstract：:To examine whether the extracellular matrix (ECM) plays a role in mediating interactions between the apical ectodermal ridge (AER) and the subjacent mesoderm in the chick wing bud, we used immunohistochemistry to locate the following tissue components during wing morphogenesis: types I and IV collagens, fibronectin, t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1141
更新日期：1994-05-01 00:00:00
abstract：:Two distinct DNA ligases are expressed during early sea urchin embryogenesis. A light form (50 kDa) is found in unfertilized eggs (oocyte form) and a heavier enzyme (110 kDa) is observed at the two-cell stage (embryonic form). The chronology of the change reveals that the embryonic form is detected 90 min after fertil...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90479-9
更新日期：1987-11-01 00:00:00
abstract：:We have examined the expression of mRNAs encoding five major neurotransmitter-synthesizing enzymes in MAH cells, a clonal cell line derived by retroviral immortalization of a rat embryonic sympathoadrenal progenitor cell. These mRNAs include tyrosine hydroxylase (TH), choline acetyltransferase (ChAT), tryptophan hydro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90313-r
更新日期：1991-11-01 00:00:00
abstract：:The NCK adaptor proteins are composed entirely of SH3 and SH2 domains and serve as protein interaction bridges for several receptors during signal transduction events. Here we report the molecular and genetic analysis of the Caenorhabditis elegans nck-1 gene. C. elegans nck-1 encodes two isoforms: NCK-1A and a shorter...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.03.019
更新日期：2011-06-01 00:00:00
abstract：:Little is known about the genetic basis of behavioral choice, such as temperature preference, especially in natural populations. Thermal preference can play a key role in habitat selection, for example in aquatic species. Examining this behavior on a genetic level requires access to individuals or populations of the s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.04.017
更新日期：2018-09-15 00:00:00
abstract：:We wish to understand how organ-specific structures assemble during embryonic development. In the present paper, we consider what determines the subapical position of the terminal web in the intestinal cells of the nematode Caenorhabditis elegans. The terminal web refers to the organelle-depleted, intermediate filamen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.003
更新日期：2004-04-15 00:00:00
abstract：:To examine whether the 200-kDa neurofilament protein (200K NFP) is involved in mechanically stabilizing axons, we studied the developmental appearance of immunoreactivity to nonphosphorylated and phosphorylated 200K NFP at the neuromuscular junction. Polyinnervated rat muscle fibers become singly innervated during the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90422-8
更新日期：1988-11-01 00:00:00
