Abstract：
:How natural variation in embryo size affects patterning of the Drosophila embryo dorsal-ventral (DV) axis is not known. Here we examined quantitatively the relationship between nuclear distribution of the Dorsal transcription factor, boundary positions for several target genes, and DV axis length. Data were obtained from embryos of a wild-type background as well as from mutant lines inbred to size select embryos of smaller or larger sizes. Our data show that the width of the nuclear Dorsal gradient correlates with DV axis length. In turn, for some genes expressed along the DV axis, the boundary positions correlate closely with nuclear Dorsal levels and with DV axis length; while the expression pattern of others is relatively constant and independent of the width of the Dorsal gradient. In particular, the patterns of snail (sna) and ventral nervous system defective (vnd) correlate with nuclear Dorsal levels and exhibit scaling to DV length; while the pattern of intermediate neuroblasts defective (ind) remains relatively constant with respect to changes in Dorsal and DV length. However, in mutants that exhibit an abnormal expansion of the Dorsal gradient which fails to scale to DV length, only sna follows the Dorsal distribution and exhibits overexpansion; in contrast, vnd and ind do not overexpand suggesting some additional mechanism acts to refine the dorsal boundaries of these two genes. Thus, our results argue against the idea that the Dorsal gradient works as a global system of relative coordinates along the DV axis and suggest that individual targets respond to changes in embryo size in a gene-specific manner.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Garcia M,Nahmad M,Reeves GT,Stathopoulos Adoi
10.1016/j.ydbio.2013.06.020subject
Has Abstractpub_date
2013-09-01 00:00:00pages
286-99issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(13)00337-0journal_volume
381pub_type
杂志文章abstract：:Cultured cells obtained from developing chick heart valvular and septal primordial tissues (cardiac cushions) and myocardium were tested for their capacity to bind, internalize, and degrade hyaluronate. A presumptive lysosomal hyaluronidase capable of hyaluronate degradation has been previously isolated and partially ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90234-3
更新日期：1984-12-01 00:00:00
abstract：:Primary sensory olfactory neurons reside in a neuroepithelium lining the nasal cavity and project topographically onto the surface of the olfactory bulb, a rostral extension of the telencephalon. Galectin-1, a bivalent galactose-binding vertebrate lectin, is expressed in the developing rodent olfactory system. In the ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0257
更新日期：1996-10-10 00:00:00
abstract：:Vertebrate pigment cells are derived from neural crest, a tissue that also forms most of the peripheral nervous system and a variety of ectomesenchymal cell types. Formation of pigment cells from multipotential neural crest cells involves a number of common developmental processes. Pigment cells must be specified; the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9840
更新日期：2000-09-15 00:00:00
abstract：:John W. Saunders, Jr. made seminal discoveries unveiling how chick embryos develop their limbs. He discovered the apical ectodermal ridge (AER), the zone of polarizing activity (ZPA), and the domains of interdigital cell death within the developing limb and determined their function through experimental analysis. Thes...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2016.11.005
更新日期：2017-09-15 00:00:00
abstract：:The serine/threonine kinase LKB1 is a master kinase that regulates a number of critical events such as cell transformation, polarization, development, stress response, and energy metabolism in metazoa. After multiple unsuccessful attempts of generating Dictyostelium lkb1-null cells, an RNAi-based knockdown approach pr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.10.006
更新日期：2011-12-15 00:00:00
abstract：:A group of proteins judged on several criteria to be yolk proteins have been isolated from a homogenate of the nematode Caenorhabditis elegans. Comparison of partial proteolysis fragments indicates that the two bands of a 170,000-dalton doublet (yp170) are closely related; bands observed at 115,000 daltons (yp115) and...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90321-4
更新日期：1983-03-01 00:00:00
abstract：:Tubes with distinct shapes and sizes are critical for the proper function of many tubular organs. Here we describe a unique phenotype caused by the loss of a novel, evolutionarily-conserved, Drosophila Smad-like protein, Expansion. In expansion mutants, unicellular and intracellular tracheal branches develop bubble-li...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.06.016
更新日期：2014-09-01 00:00:00
abstract：:The mammalian Cul4 genes, Cul4A and Cul4B, encode the scaffold components of the cullin-based E3 ubiquitin ligases. The two Cul4 genes are functionally redundant. Recent study indicated that mice expressing a truncated CUL4A that fails to interact with its functional partner ROC1 exhibit no developmental phenotype. We...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.01.028
更新日期：2011-04-15 00:00:00
abstract：:Closure of the cranial neural tube depends on normal development of the head mesenchyme. Homozygous-mutant embryos for the ENU-induced open mind (opm) mutation exhibit exencephaly associated with defects in head mesenchyme development and dorsal-lateral hinge point formation. The head mesenchyme in opm mutant embryos ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.03.018
更新日期：2007-06-01 00:00:00
abstract：:A new method is presented for preparation of highly purified plasma membranes from fertilized sea urchin eggs. The purified plasma membranes are in vesicle form and are highly enriched in ouabain inhibitable, Na+/K+ ATPase activity. Analysis of membrane proteins by sodium dodecyl sulfate-gel electrophoresis indicates ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90106-9
更新日期：1983-06-01 00:00:00
abstract：:Effective transgenesis methods have been successfully employed in many organisms including zebrafish. However, accurate spatiotemporal control of transgene expression is still difficult to achieve. Here we describe a system for chemical-inducible gene expression and demonstrate its feasibility for generating transgeni...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.042
更新日期：2008-08-01 00:00:00
abstract：:Developing vertebrate limbs are often utilized as a model for studying pattern formation and morphogenetic cell death. Herein, we report that conditional deletion of Rac1, a member of the Rho family of proteins, in mouse limb bud mesenchyme led to skeletal deformities in the autopod and soft tissue syndactyly, with th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.09.014
更新日期：2009-11-15 00:00:00
abstract：:The development of asymmetric patterns along biologically relevant axes is a hallmark of many vertebrate organs or structures. One example is the sensory epithelium of the mammalian auditory system. Two distinct types of mechanosensory hair cells (inner and outer) and at least six types of associated supporting cells ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.06.003
更新日期：2019-09-15 00:00:00
abstract：:The Endo16 gene codes for an RGD-containing calcium-binding protein that is found on the basal surfaces and in the extracellular matrix of cells of the invaginating archenteron during sea urchin gastrulation. Previously, we have shown that Endo16 is a single copy gene and we have determined the coding sequence and ana...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0247
更新日期：1996-10-10 00:00:00
abstract：:The unc-52 gene of Claenorhabditis elegans encodes a homologue of the basement membrane heparan sulfate proteoglycan perlecan. Viable alleles reduce the abundance of UNC-52 in late larval stages and increase the frequency of distal tip cell (DTC) migration defects caused by mutations disrupting the UNC-6/netrin guidan...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00014-9
更新日期：2003-04-01 00:00:00
abstract：:Soluble N-ethylmalameide-sensitive factor attachment protein receptor (SNARE) proteins are present in mammalian sperm and could be involved in critical membrane fusion events during fertilization, namely the acrosome reaction. Vesicle-associated membrane protein/synaptobrevin, a SNARE on the membrane of a vesicular ca...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9745
更新日期：2000-07-01 00:00:00
abstract：:Hox genes are crucial players in the generation and pattering of the vertebrate trunk and posterior body during embryogenesis. Their initial expression takes place shortly after the establishment of the primitive streak, in the posterior-most part of the mouse embryo and is a determinant step for setting up the defini...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2017.02.009
更新日期：2017-08-15 00:00:00
abstract：:Mouse embryo limb cells carrying either the brachypodism (bpH/bpH) mutation or its wild-type (+/+) allele were tested for their ability to accumulate cyclic AMP in response to prostaglandin E2 (PGE2) between Embryonic Days E12 and E14. Mutant cells exhibited a precocious increase in cyclic AMP. In the absence of PGE2 ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90372-p
更新日期：1990-12-01 00:00:00
abstract：:Early spermatids contain roughly 1000-fold more TATA-binding protein (TBP) mRNA than do somatic cells. The appearance of TBP-overexpressing spermatids in the developing testis is accompanied by a large increase in whole-organ levels of total RNA and of poly(A)+ RNA per cell. Whereas somatic cells initiate transcriptio...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8514
更新日期：1997-04-01 00:00:00
abstract：:Intercellular communication mediated by gap junctions is important for tissue homeostasis in the avascular lens, and extensive areas of gap junctions form between fiber cells during fiber cell differentiation and lens development. We examined the role of the calcium-dependent cell adhesion molecule, N-cadherin, in the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0237
更新日期：1996-10-10 00:00:00
abstract：:Amphibian embryos from the genus Xenopus are among the best species for understanding early vertebrate development and for studying basic cell biological processes. Xenopus, and in particular the diploid Xenopus tropicalis, is also ideal for functional genomics. Understanding the behavior of genes in this accessible m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.04.023
更新日期：2004-07-15 00:00:00
abstract：:N-cadherin is a Ca2+-dependent cell-cell adhesion molecule, which was identified in brain cells of mouse and chicken. In the present study, we have determined the pattern of expression of N-cadherin in chicken embryos at various stages by means of immunohistochemistry. N-cadherin was expressed in cells derived from al...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90119-9
更新日期：1987-03-01 00:00:00
abstract：:The s-SHIP protein is a shorter isoform of the longer SHIP1 protein and lacks the N-terminal SH2 domain region contained in SHIP1. s-SHIP is expressed in ES cells and in enriched bone marrow stem cells, and may be controlled by a promoter within intron 5 of the ship1 gene. We therefore examined the potential specifici...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.04.032
更新日期：2005-07-15 00:00:00
abstract：:Previous studies have established that embryonal carcinoma (EC) cells produce several different growth factors, but express few, if any, receptors for epidermal growth factor, platelet-derived growth factor, or transforming growth factor type-beta. In this study, the production and utilization of fibroblast growth fac...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90161-3
更新日期：1988-09-01 00:00:00
abstract：:Genome stability relies notably on the integrity of centrosomes and on the mitotic spindle they organize. Structural and numerical centrosome aberrations are frequently observed in human cancer, and there is increasing evidence that centrosome amplification can promote tumorigenesis. Here, we use C. elegans seam cells...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.01.001
更新日期：2018-02-15 00:00:00
abstract：:CREB-2 (also called ATF4, TAXREB67, or C/ATF) is an evolutionarily conserved member of the CREB/ATF family of basic-leucine zipper transcription factors. CREB-2 is expressed ubiquitously in the adult mouse and can function as both a transcriptional activator and a repressor. However, little was understood about the no...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9699
更新日期：2000-06-01 00:00:00
abstract：:Most, if not all, endocrine cells seem capable of synthesizing and storing more than one hormone. Such cellular colocalization of hormones can be due either to the presence of two or more specific granules within the cells or to colocalization of the hormones within a single granule. The present study was performed to...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90122-w
更新日期：1992-10-01 00:00:00
abstract：:Modulation of reactive oxygen species (ROS) plays a key role in signal transduction pathways. Selenoproteins act controlling the redox balance of the cell. We have studied how the alteration of the redox balance caused by patufet (selD(ptuf)), a null mutation in the Drosophila melanogaster selenophosphate synthetase 1...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0389
更新日期：2001-10-01 00:00:00
abstract：:In the development of mammalian organs, a rapid and robust expansion of the parenchymal compartment must occur following allocation of organ primordia. This expansion must be regulated so that sufficient tissue mass is generated for further organization into functional tissues. The discovery that mosaic patches in the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1274
更新日期：1994-10-01 00:00:00
abstract：:BMPRIA is a receptor for bone morphogenetic proteins with high affinity for BMP2 and BMP4. Mouse embryos lacking Bmpr1a fail to gastrulate, complicating studies on the requirements for BMP signaling in germ layer development. Recent work shows that BMP4 produced in extraembryonic tissues initiates gastrulation. Here w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.048
更新日期：2004-06-01 00:00:00
