Abstract：
:Activation and role of mitogen-activated protein (MAP) kinase (MAPK) during mitosis are still matters of controversy in early embryos. We report here that an ERK-like protein is present and highly phosphorylated in unfertilized sea urchin eggs. This MAPK becomes dephosphorylated after fertilization and a small pool of it is transiently reactivated during mitosis. The phosphorylated ERK-like protein is localized to the nuclear region and then to the mitotic poles and the mitotic spindle. Treatment of eggs after fertilization with two different MEK inhibitors, PD 98059 and U0126, at low concentrations capable to selectively induce dephosphorylation of this ERK-like protein, or expression of a dominant-negative MEK1/2, perturbed mitotic progression. Our results suggest that an ERK-like cascade is part of a control mechanism that regulates mitotic spindle formation and the attachment of chromosomes to the spindle during the first mitosis of the sea urchin embryo.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Zhang WL,Huitorel P,Glass R,Fernandez-Serra M,Arnone MI,Chiri S,Picard A,Ciapa Bdoi
10.1016/j.ydbio.2005.03.008subject
Has Abstractpub_date
2005-06-01 00:00:00pages
192-206issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(05)00175-2journal_volume
282pub_type
杂志文章abstract：:A reexamination of the cDNA clones of the Strongylocentrotus purpuratus sea urchin egg receptor for sperm resulted in several important changes to the sequence. By using both rapid amplification of cDNA ends (RACE) and Northern blot analysis for confirmation, the corrected deduced amino acid sequence was shown to lack...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8504
更新日期：1997-04-01 00:00:00
abstract：:Sperm-egg interaction was investigated in mouse eggs freed from the zona pellucida and injected with Clostridium difficile toxin B, the inhibitor of Rho family small G proteins. Toxin B reduced in a dose-dependent manner the percentage of eggs associated with sperm fusion on the surface or sperm nucleus decondensation...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00273-2
更新日期：2003-08-15 00:00:00
abstract：:Plakoglobin and beta-catenin are cytoplasmic proteins associated with the intracellular plaques of cell adhesive junctions. While plakoglobin is present in both adherens junctions and desmosomes, beta-catenin is associated with the cadherins that accumulate only in adherens junctions. Both beta-catenin and plakoglobin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90118-z
更新日期：1992-10-01 00:00:00
abstract：:We have isolated two related Xenopus homologues of the homeotic zinc finger protein Teashirt1 (Tsh1), XTsh1a and XTsh1b. While Drosophila teashirt specifies trunk identity in the fly, the developmental relevance of vertebrate Tsh homologues is unknown. XTsh1a/b are expressed in prospective trunk CNS throughout early n...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.06.041
更新日期：2006-10-01 00:00:00
abstract：:Temporally-regulated maternal RNA translation is essential for embryonic development, with defective degradation resulting in stalled 2-cell embryos. We show that DDX1, a DEAD box protein implicated in RNA transport, may be a key regulator of maternal RNA utilization. DDX1 protein localizes exclusively to cytoplasmic ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.07.014
更新日期：2019-11-15 00:00:00
abstract：:A gene regulatory network subcircuit comprising the otx, wnt8, and blimp1 genes accounts for a moving torus of gene expression that sweeps concentrically across the vegetal domain of the sea urchin embryo. Here we confirm by mutation the inputs into the blimp1cis-regulatory module predicted by network analysis. Its es...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.10.042
更新日期：2008-01-15 00:00:00
abstract：:The Drosophila segment polarity gene known as shaggy or zeste white 3 encodes a ubiquitously expressed serine-threonine protein kinase which is critical for a number of important developmental processes. In the developing wing blade, clones of cells lacking the normal shaggy-zeste white 3 product form dense tufts of m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1081
更新日期：1994-03-01 00:00:00
abstract：:During endochondral bone formation, vascular invasion initiates the replacement of avascular cartilage by bone. We demonstrate herein that the cartilage-specific overexpression of VEGF-A(164) in mice results in the hypervascularization of soft connective tissues away from cartilage. Unexpectedly, perichondrial tissue ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.05.552
更新日期：2009-08-15 00:00:00
abstract：:An increase in the incidence of aneuploidy is well documented with increasing maternal age, in particular in human females. Remarkably, little is known regarding the underlying molecular basis for the age-associated increase in aneuploidy, which is a major source of decreased fertility in humans. Using mouse as a mode...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.01.048
更新日期：2008-04-15 00:00:00
abstract：:The zebrafish T-box transcription factors spadetail (spt) and the brachyury ortholog no tail (ntl) are together essential for posterior mesoderm formation. In addition to being functionally redundant, spt and ntl also genetically interact with zygotic mutant alleles of one-eyed pinhead (Zoep), leading to synergistic m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.09.008
更新日期：2003-12-15 00:00:00
abstract：:A genetically complex mutant, two-faced (tfd), which causes the production of extra eye and antennal tissue on the dorsal head cuticle, has been analyzed for connectivity to and projection patterns in the central brain of nerves derived from these extra structures. It has been found that the extra antennal nerves freq...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90370-7
更新日期：1985-01-01 00:00:00
abstract：:A glycoprotein from the liver, which shares epitopes with chorion (egg envelope or zona pellucida) glycoproteins, is present only in the spawning female fish, Oryzias latipes, under natural conditions. This spawning female-specific (SF) substance is distinct from vitellogenin but closely resembles a major glycoprotein...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90301-1
更新日期：1989-05-01 00:00:00
abstract：:Using lineage tracers, we recently showed dorsal and ventral ectodermal compartments along the sides of the body in chick embryos. The compartments are formed both in presumptive limb-forming regions where they position the apical ridge and also in presumptive interlimb (flank). Here we show, using a novel technique c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9659
更新日期：2000-06-15 00:00:00
abstract：:A cell surface protein (3B11) is differentially expressed in the embryonic labial segment of Manduca as two circular monolayers of epithelial cells invaginate and segregate from surrounding epithelial cells. The cells that invaginate and preferentially express 3B11 represent the presumptive prothoracic glands. These c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.8437
更新日期：1997-01-01 00:00:00
abstract：:Vertebrate voluntary muscles are composed of myotubes and connective tissue cells. These two cell types have different embryonic origins: myogenic cells arise from paraxial mesoderm, while in the head many of the connective tissues are formed by neural crest cells. The objective of this research was to study interacti...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90138-7
更新日期：1986-08-01 00:00:00
abstract：:Repulsion plays a fundamental role in the establishment of a topographic map of the chick retinotectal projections. This has been highlighted by studies demonstrating the role of opposing gradients of the EphA3 receptor tyrosine kinase on retinal axons and two of its ligands, ephrin-A2 and ephrin-A5, in the tectum. We...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0268
更新日期：2001-06-15 00:00:00
abstract：:Hedgehog proteins have been implicated in the control of myogenesis in the medial vertebrate somite. In the mouse, normal epaxial expression of the myogenic transcription factor gene myf5 is dependent on Sonic hedgehog. Here we examine in zebrafish the interaction between Hedgehog signals, the expression of myoD famil...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0193
更新日期：2001-08-01 00:00:00
abstract：:Ovarian germ cell survival is dependent upon the formation of primordial follicles, which occurs during fetal life in the human. Activin contributes to germ cell proliferation and survival at this time. SMADs2 and 3 are central elements in the activin signalling pathway and thus indicate sites of activin action. We ha...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.11.026
更新日期：2008-02-01 00:00:00
abstract：:The first step in the development of the Drosophila optic medullar primordia is the expansion of symmetrically dividing neuroepithelial cells (NEs); this step is then followed by the appearance of asymmetrically dividing neuroblasts (NBs). However, the mechanisms responsible for the change from NEs to NBs remain uncle...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.12.044
更新日期：2011-03-01 00:00:00
abstract：:Gap junctions are intercellular channels made of connexins (Cxs) that allow direct communication between adjacent cells. Modulation of Cxs has been associated with abnormal development and function of the mammary gland and breast cancer. However, the mechanisms underlying their expression during normal mammary gland a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.06.011
更新日期：2016-08-01 00:00:00
abstract：:Cyclic protein-2 (CP-2) is secreted in vitro in substantial amounts by mature rat Sertoli cells in intact Stage VI and Stage VII seminiferous tubules. This stage-dependent secretion has led us to postulate that the biosynthesis of this molecule is stimulated by germ cells at a specific state of development. In order t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90412-5
更新日期：1988-11-01 00:00:00
abstract：:Cleft palate is one of the most common craniofacial congenital defects in humans. It is associated with multiple genetic and environmental risk factors, including mutations in the genes encoding signaling molecules in the sonic hedgehog (Shh) pathway, which are risk factors for cleft palate in both humans and mice. Ho...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.07.003
更新日期：2018-09-01 00:00:00
abstract：:The enteric nervous system (ENS) of the larval moth Manduca sexta consists of two small ganglia and several nerve networks that lie superficially along the alimentary tract. Within this system are approximately 600 neurons that exhibit a spectrum of biochemical and morphological characteristics and that express these ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(89)80039-9
更新日期：1989-01-01 00:00:00
abstract：:Endogenous patterns of retinoic acid (RA) signaling in avian cardiac morphogenesis were characterized by localized expression of a key RA-synthetic enzyme, RALDH2, which displayed a biphasic pattern during heart development. RALDH2 immunoreactivity was initially apparent posterior to Hensen's node of stage 5-6 embryos...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9588
更新日期：2000-03-01 00:00:00
abstract：:The lizards are evolutionarily the closest vertebrates to humans that demonstrate the ability to regenerate entire appendages containing cartilage, muscle, skin, and nervous tissue. We previously isolated PAX7-positive cells from muscle of the green anole lizard, Anolis carolinensis, that can differentiate into multin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.08.037
更新日期：2018-01-15 00:00:00
abstract：:The interstitial cells of hydra form a multipotent stem cell system, producing terminally differentiated nerve cells and nematocytes during asexual growth. Under well-fed conditions the interstitial cell population doubles in size every 4 days. We have investigated the possible role of nerve cells in regulating this b...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90089-2
更新日期：1985-08-01 00:00:00
abstract：:Thrombospondin type 1 repeat (TSR) superfamily members regulate diverse biological activities ranging from cell motility to inhibition of angiogenesis. In this study, we verified that mouse protein O-fucosyltransferase-2 (POFUT2) specifically adds O-fucose to TSRs. Using two Pofut2 gene-trap lines, we demonstrated tha...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.07.008
更新日期：2010-10-01 00:00:00
abstract：:In this study, we present evidence that neurogenic cells inhibit the differentiation of cardiogenic cells. When cells of the entire area pellucida at stage 5 were dissociated and reaggregated, the aggregates differentiated into neural tissues and other structures of any germ layer origin, except for heart tissues, des...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1266
更新日期：1995-09-01 00:00:00
abstract：:The ability to express exogenous reporter genes in intact, externally developing embryos, such as Xenopus, is a powerful tool for characterizing the activity of cis-regulatory gene elements during development. Although methods exist for generating transgenic Xenopus lines, more simplified methods for use with F0 anima...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.11.010
更新日期：2015-02-01 00:00:00
abstract：:Interstitial cell migration was characterized in Hydra vulgaris (formerly H. attenuata) and the effects of axial position and tissue injury on migration were investigated. Migrating cells were labeled with the thymidine analog, bromodeoxyuridine, and grafted into unlabeled host polyps. Alternatively, cells were labele...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90177-k
更新日期：1990-03-01 00:00:00
