Abstract:
:The Mesp bHLH genes play a conserved role during segmental patterning of the mesoderm in the vertebrate embryo by specifying segmental boundaries and anteroposterior (A-P) segmental polarity. Here we use a xenotransgenic approach to compare the transcriptional enhancers that drive expression of the Mesp genes within segments of the presomitic mesoderm (PSM) of different vertebrate species. We find that the genomic sequences upstream of the mespb gene in the pufferfish Takifugu rubripes (Tr-mespb) are able to drive segmental expression in transgenic Xenopus embryos while those from the Xenopus laevis mespb (Xl-mespb) gene drive segmental expression in transgenic zebrafish. In both cases, the anterior segmental boundary of transgene expression closely matches the expression of the endogenous Mesp genes, indicating that many inputs into segmental gene expression are highly conserved. By contrast, we find that direct retinoic acid (RA) regulation of endogenous Mesp gene expression is variable among vertebrate species. Both Tr-mespb and Xl-mespb are directly upregulated by RA, through a complex, distal element. By contrast, RA represses the zebrafish Mesp genes. We show that this repression is mediated, in part, by RA-mediated activation of the Ripply genes, which together with Mesp genes form an RA-responsive negative feedback loop. These observations suggest that variations in a direct response to RA input may allow for changes in A-P patterning of the segments in different vertebrate species.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Moreno TA,Jappelli R,Izpisúa Belmonte JC,Kintner Cdoi
10.1016/j.ydbio.2007.12.038subject
Has Abstractpub_date
2008-03-15 00:00:00pages
317-30issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(07)01596-5journal_volume
315pub_type
杂志文章abstract::Skeletal muscles of the avian limb are derived from mononucleated myogenic precursor cells (myoblasts) that migrate into the somatopleural mesoderm of the developing limb bud from the ventrolateral dermomyotome of limb adjacent somites. In the present study, we utilized replication-deficient lacZ-encoding retroviruses...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(02)00028-3
更新日期:2003-01-15 00:00:00
abstract::To investigate the molecular regulation of embryonic somite formation and development, we have cloned the full-length cDNA and characterized the embryonic expression profile of chicken Paraxis, a member of a novel family of basic helix-loop-helix (bHLH) proteins, which has been suggested to play a role in paraxial mes...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8663
更新日期:1997-09-01 00:00:00
abstract::The gastrointestinal (GI) tract of metazoans is lined by a series of regionally distinct epithelia. To maintain structure and function of the GI tract, regionally diversified differentiation of somatic stem cell (SC) lineages is critical. The adult Drosophila midgut provides an accessible model to study SC regulation ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.08.027
更新日期:2016-11-15 00:00:00
abstract::Oocyte maturation (meiosis reinitiation) in starfish is induced by the natural hormone 1-methyladenine (1-MeAde). Oocytes of Evasterias troschelii contain 0.43 pmole cyclic AMP/mg protein and 0.47 pmole cyclic GMP/mg protein. Upon stimulation by 1-MeAde the oocytes undergo a moderate (10-30%) decrease in their cAMP co...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90166-7
更新日期:1987-06-01 00:00:00
abstract::Notch signaling has a fundamental role in stem cell maintenance and in cell fate choice in the intestine of different species. Canonically, Notch signaling represses the expression of transcription factors of the achaete-scute like (ASCL) or atonal related protein (ARP) families. Identifying the ARP/ASCL genes express...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.01.011
更新日期:2013-04-15 00:00:00
abstract::During the last stages of fetal life, the immature epithelial cells of the rat lung alveolus develop the properties of mature type 2 cells. Adult type 2 cells rapidly lose these same properties when isolated and maintained in cell culture. We have examined the synthesis of cytokeratin proteins by adult type 2 cells as...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90396-x
更新日期:1988-10-01 00:00:00
abstract::alpha11beta1 integrin constitutes a recent addition to the integrin family. Here, we present the first in vivo analysis of alpha11 protein and mRNA distribution during human embryonic development. alpha11 protein and mRNA were present in various mesenchymal cells around the cartilage anlage in the developing skeleton ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0363
更新日期:2001-09-01 00:00:00
abstract::Temporally-regulated maternal RNA translation is essential for embryonic development, with defective degradation resulting in stalled 2-cell embryos. We show that DDX1, a DEAD box protein implicated in RNA transport, may be a key regulator of maternal RNA utilization. DDX1 protein localizes exclusively to cytoplasmic ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.07.014
更新日期:2019-11-15 00:00:00
abstract::Thrombospondin type 1 repeat (TSR) superfamily members regulate diverse biological activities ranging from cell motility to inhibition of angiogenesis. In this study, we verified that mouse protein O-fucosyltransferase-2 (POFUT2) specifically adds O-fucose to TSRs. Using two Pofut2 gene-trap lines, we demonstrated tha...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.07.008
更新日期:2010-10-01 00:00:00
abstract::Fertilization results in the tyrosine phosphorylation of several egg proteins within minutes of sperm-egg binding and inhibitor studies have shown that tyrosine kinase activity is required for many aspects of egg activation. The present study demonstrates the presence of p59c-fyn kinase in the sea urchin egg and exami...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0073
更新日期:1996-03-15 00:00:00
abstract::Using optical imaging assays, we investigated the dynamics of acetylcholine receptors (AChRs) at laminin-associated clusters on cultured myotubes in the absence or presence of the nerve-derived clustering factor, agrin. Using fluorescence recovery after photobleaching (FRAP) on fluorescent bungarotoxin-labeled recepto...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.09.041
更新日期:2005-12-01 00:00:00
abstract::Proper calcium channel and insulin signaling are essential for normal brain development. Leaner mice with a mutation in the P/Q-type voltage-gated calcium channel, Cacna1a, develop cerebellar atrophy and mutations in the homologous human gene are associated with increased migraine and seizure tendency. Similarly, abno...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.06.005
更新日期:2006-10-01 00:00:00
abstract::Mammalian heart development involves complex morphogenetic events which lead to the formation of fully separated left and right atrial and ventricular chambers from a tubular heart. Separation of left and right ventricular chambers is dependent on a single structure, the interventricular septum (IVS), which has both m...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.02.045
更新日期:2006-06-15 00:00:00
abstract::Large steady electrical currents traverse Drosophila follicles in vitro as well as permeabilized eggs. During the period of main follicle growth (stages 9-11), these currents enter the anterior or nurse cell end of the follicles. This inward current acts like a sodium ion influx with some calcium involvement. During t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90013-2
更新日期:1985-03-01 00:00:00
abstract::The expression of the neural cell adhesion molecules N-CAM and L1 was investigated in the olfactory system of the mouse using immunocytochemical and immunochemical techniques. In the olfactory epithelium, globose basal cells and olfactory neurons were stained by the polyclonal N-CAM antibody reacting with all three co...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90397-1
更新日期:1988-10-01 00:00:00
abstract::In Xenopus gastrulation, the involuting mesodermal and non-involuting ectodermal cells remain separated from each other, undergoing convergent extension. Here, we show that Eph-ephrin signaling is crucial for the tissue separation and convergence during gastrulation. The loss of EphA4 function results in aberrant gast...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.12.012
更新日期:2011-02-15 00:00:00
abstract::Notch pathway is crucial for stem/progenitor cell maintenance, growth and differentiation in a variety of tissues. Using a transgenic cell ablation approach, we found in our previous study that cells expressing Notch1 are crucial for prostate early development and re-growth. Here, we further define the role of Notch s...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.11.009
更新日期:2006-02-01 00:00:00
abstract::Integrins are expressed on the surface of some vertebrate eggs where they are thought to have a role in fertilization. The objective of this study is to determine if integrins are expressed on sea urchin eggs. The alphaB and betaC subunits were cloned using the homology polymerase chain reaction. Monoclonal and polycl...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9910
更新日期:2000-11-15 00:00:00
abstract::Unlike adult mammals, adult frogs regrow their optic nerve following a crush injury, making Xenopus laevis a compelling model for studying the molecular mechanisms that underlie neuronal regeneration. Using Translational Ribosome Affinity Purification (TRAP), a method to isolate ribosome-associated mRNAs from a target...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.06.003
更新日期:2017-06-15 00:00:00
abstract::Biologists have long recognized that dramatic bending of a cell sheet may be driven by even modest shrinking of the apical sides of cells. Cell shape changes and tissue movements like these are at the core of many of the morphogenetic movements that shape animal form during development, driving processes such as gastr...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2009.09.009
更新日期:2010-05-01 00:00:00
abstract::The sensory neurons of the Drosophila wing differentiate during the initial stages of metamorphosis, appearing in the imaginal wing disc as it everts and flattens. These identifiable neurons arise in a stereotyped sequence, and lay down a specific pattern of axon bundles which travel proximally to the CNS. In several ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90044-2
更新日期:1985-04-01 00:00:00
abstract::Caenorhabditis elegans embryonic elongation depends on both epidermal and muscle cells. The hemidesmosome-like junctions, commonly called fibrous organelles (FOs), that attach the epidermis to the extracellular matrix ensure muscle anchoring to the cuticular exoskeleton and play an essential role during elongation. To...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.11.025
更新日期:2011-02-15 00:00:00
abstract::In the zebrafish embryo, cells fated to give rise to the rostral brain move in a concerted fashion and retain tissue coherence during morphogenesis. We demonstrate here that Otx proteins have a dramatic effect on cell-cell interactions when expressed ectopically in the zebrafish embryo. Injection of zebrafish Otx1 or ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9771
更新日期:2000-07-15 00:00:00
abstract::Adhesive extracellular matrix (ECM) molecules appear to play roles in the migration of neural crest cells, and may also provide cues for differentiation of these cells into a variety of phenotypes. We are studying the influences of specific ECM components on crest differentiation at the levels of both individual cells...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90112-v
更新日期:1990-09-01 00:00:00
abstract::Malpighian tubules are the osmoregulatory and detoxifying organs of Drosophila and its proper development is critical for the survival of the organism. They are made up of two major cell types, the ectodermal principal cells and mesodermal stellate cells. The principal and stellate cells are structurally and physiolog...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.11.003
更新日期:2015-02-15 00:00:00
abstract::We have isolated Xbr-1, a novel Xenopus homeobox gene that defines a new class of homeobox genes, distantly related to the Drosophila BarH1 and BarH2 genes. Xbr-1 is predominantly expressed in the developing Xenopus eye, starting as early as the neural plate stage. At early stages of eye development XBR-1 is expressed...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0055
更新日期:1996-02-25 00:00:00
abstract::The rabbit sperm membrane autoantigen RSA-1 is a sialoglycoprotein of 13,000 daltons which first appears on the surface of pachytene spermatocytes. Using specific antiserum to RSA-1 the antigen has been localized by immunofluorescence and immunoperoxidase staining. On testicular cells labeled at 37 degrees C, RSA-1 is...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(81)90400-0
更新日期:1981-06-01 00:00:00
abstract::Rho GTPases control signal transduction pathways that link cell surface receptors to a variety of intracellular responses. They are best known as regulators of the actin cytoskeleton, but in addition they control cell polarity, gene expression, microtubule dynamics and vesicular trafficking. Through these diverse func...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2003.06.003
更新日期:2004-01-01 00:00:00
abstract::Biomineralization, the biologically controlled formation of mineral deposits, is of widespread importance in biology, medicine, and engineering. Mineralized structures are found in most metazoan phyla and often have supportive, protective, or feeding functions. Among deuterostomes, only echinoderms and vertebrates pro...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.07.047
更新日期:2006-12-01 00:00:00
abstract::Monoclonal antibody 4F4, which was raised against a cell suspension of embryonic rat forebrain, reacts with acidic glycolipids and several high-molecular-weight glycoproteins in rodent brain. The major reactive glycolipid is maximally expressed at Embryonic Day 15 (E15) and is no longer detectable at Postnatal Day 14 ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90104-7
更新日期:1987-03-01 00:00:00