Abstract：
:Notch pathway is crucial for stem/progenitor cell maintenance, growth and differentiation in a variety of tissues. Using a transgenic cell ablation approach, we found in our previous study that cells expressing Notch1 are crucial for prostate early development and re-growth. Here, we further define the role of Notch signaling in regulating prostatic epithelial cell growth and differentiation using biochemical and genetic approaches in ex vivo or in vivo systems. Treatment of developing prostate grown in culture with inhibitors of gamma-secretase/presenilin, which is required for Notch cleavage and activation, caused a robust increase in proliferation of epithelial cells co-expressing cytokeratin 8 and 14, lack of luminal/basal layer segregation and dramatically reduced branching morphogenesis. Using conditional Notch1 gene deletion mouse models, we found that inactivation of Notch1 signaling resulted in profound prostatic alterations, including increased tufting, bridging and enhanced epithelial proliferation. Cells within these lesions co-expressed both luminal and basal cell markers, a feature of prostatic epithelial cells in predifferentiation developmental stages. Microarray analysis revealed that the gene expression in a number of genetic networks was altered following Notch1 gene deletion in prostate. Furthermore, expression of Notch1 and its effector Hey-1 gene in human prostate adenocarcinomas were found significantly down-regulated compared to normal control tissues. Taken together, these data suggest that Notch signaling is critical for normal cell proliferation and differentiation in the prostate, and deregulation of this pathway may facilitate prostatic tumorigenesis.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Wang XD,Leow CC,Zha J,Tang Z,Modrusan Z,Radtke F,Aguet M,de Sauvage FJ,Gao WQdoi
10.1016/j.ydbio.2005.11.009subject
Has Abstractpub_date
2006-02-01 00:00:00pages
66-80issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(05)00786-4journal_volume
290pub_type
杂志文章abstract：:Tyrosinase is the rate-limiting enzyme for the production of melanin pigmentation. In the mouse and other animals, homozygous null mutations in the Tyrosinase gene (Tyr) result in the absence of pigmentation, i.e. albinism. Here we used the CRISPR/Cas9 system to generate mono- and bi-allelic null mutations in the Tyr ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.06.017
更新日期：2014-09-01 00:00:00
abstract：:P19 embryonal carcinoma cells are multipotential stem cells that differentiate into striated muscle as well as some other cell types when aggregated and exposed to dimethyl sulfoxide (DMSO). Immunofluorescence experiments using monospecific antibodies indicated that the majority of muscle cells were mononucleate and c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90202-t
更新日期：1990-04-01 00:00:00
abstract：:Carbohydrate chains on the t12 mutant embryos were analyzed. No abnormalities of the synthesis of the carbohydrate chains were observed in the t12 homozygotes until the late morula stage, when radiolabeled carbohydrate chains were analyzed by Sephadex G-50 column chromatography. Furthermore, polarization of the Con A ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90309-0
更新日期：1988-08-01 00:00:00
abstract：:A complex regulatory cascade is required for normal cardiac development, and many aspects of this network are conserved from Drosophila to mammals. In Drosophila, the seven-up (svp) gene, an ortholog of the vertebrate chick ovalbumin upstream promoter transcription factors (COUP-TFI and II), is initially activated in ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.10.025
更新日期：2007-02-15 00:00:00
abstract：:It has long been hypothesized that in fishes the contents of cortical granules are involved in the hardening of egg envelope following fertilization. We previously purified the egg envelope hardening initiation factor from the exudates released from activated medaka (Oryzias latipes) eggs and tentatively termed this p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.09.016
更新日期：2012-12-15 00:00:00
abstract：:We have compared insulin binding by heart cells at 7 and 14 days of development. Species specificity, optimum pH, temperature relationships, and time to equilibrium for binding of insulin were the same in both 7 and 14-day systems. Curvilinear Scatchard plots for chicken insulin binding were demonstrated. Binding affi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(81)90410-3
更新日期：1981-06-01 00:00:00
abstract：:The remarkable regenerative capacity displayed by plants and various vertebrates, such as amphibians, is largely based on the capability of somatic cells to undergo dedifferentiation. In this process, mature cells reverse their state of differentiation and acquire pluripotentiality--a process preceding not only reentr...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2003.12.027
更新日期：2004-04-01 00:00:00
abstract：:To identify guanine nucleotide binding proteins (G-proteins) in sea urchin eggs and to investigate their role in signal transduction at fertilization, we used cholera toxin (CTX) and pertussis toxin (PTX), which catalyze the specific ADP-ribosylation of G-proteins. Cell surface complex, consisting of plasma membranes ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90260-0
更新日期：1987-04-01 00:00:00
abstract：:Gr16 (chromosome 2, 6.9 cM) is a dominant, grey egg mutation of the domestic silkmoth, Bombyx mori, which produces an opaque instead of a translucent chorion. To determine the underlying cause of this defect, we examined the effects of the mutation on chorion ultrastructure, protein accumulation, and biosynthesis patt...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1112
更新日期：1993-05-01 00:00:00
abstract：:We investigated the influence of the growth surface on the direction of Xenopus spinal neurite growth in the presence of a dc electric field of physiological magnitude. The direction of galvanotropism was determined by the substratum; neurites grew toward the negative electrode (cathode) on untreated Falcon tissue cul...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9039
更新日期：1998-11-15 00:00:00
abstract：:Rapidly growing human teratocarcinoma cells (Tera-2) can be induced to differentiate into quiescent, nontumorigenic cells expressing neuronal markers. To more closely mimic the in vivo conditions for tumor growth, we grew Tera-2 cells in three-dimensional collagen gel cultures. The undifferentiated cells proliferated ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1016
更新日期：1994-01-01 00:00:00
abstract：:Developing cardiac myocytes divide a limited number of times before they stop and terminally differentiate, but the mechanism that stops their division is unknown. To help study the stopping mechanism, we defined conditions under which embryonic rat cardiac myocytes cultured in serum-free medium proliferate and exit t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9524
更新日期：1999-12-15 00:00:00
abstract：:The patterns of myosin heavy chain (MyHC) isoform expression in the embryo and in the adult mouse are reasonably well characterized and quite distinct. However, little is known about the transition between these two states, which involves major decreases and increases in the expression of several MyHC genes. In the pr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9974
更新日期：2001-01-15 00:00:00
abstract：:In C. elegans the 4-cell stage blastomere EMS is an endomesodermal precursor. Its anterior daughter, MS, makes primarily mesodermal cells, while its posterior daughter E generates the entire intestine. The gene regulatory network underlying specification of MS and E has been the subject of study for more than 15 years...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.09.042
更新日期：2010-04-15 00:00:00
abstract：:Copyright ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8954
更新日期：1998-07-15 00:00:00
abstract：:The MSP domain is a conserved immunoglobulin-like structure that is important for C. elegans reproduction and human motor neuron survival. C. elegans MSPs are the most abundant proteins in sperm, where they function as intracellular cytoskeletal proteins and secreted hormones. Secreted MSPs bind to multiple receptors ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.03.026
更新日期：2010-06-01 00:00:00
abstract：:Brain 4 (Brn4/Pou3f4) and Pax6 are POU-homeodomain and paired-homeodomain transcription factors, respectively, that are expressed in the brain and the glucagon-expressing cells in the pancreas. Brn4 expression begins at embryonic day 10 in the pancreas, just before pax6 and both appear in the glucagon immunoreactive c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.12.008
更新日期：2004-04-01 00:00:00
abstract：:Connective tissue outgrowths of neonatal muscle onto a substratum of bone matrix differentiate into cartilage in response to a bone morphogenetic protein (BMP). The BMP can be separated from bone matrix by extraction with 4 M guanidine hydrochloride (GuHCl) or degraded in situ by endogenous proteolytic enzymes to deac...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90339-9
更新日期：1988-12-01 00:00:00
abstract：:The Toll signaling pathway functions in several Drosophila processes, including dorsal-ventral pattern formation and the immune response. Here, we demonstrate that this pathway is required in the epidermis for proper muscle development. Previously, we showed that the zygotic Toll protein is necessary for normal muscle...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8915
更新日期：1998-07-01 00:00:00
abstract：:The regulation of early embryonic development in the amphibian Xenopus laevis depends largely upon translational and post-translational regulatory mechanisms to direct the complex cytodifferentiations that take place during early cleavage and blastula formation. The cell cycle dependence of protein synthesis was exami...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90460-k
更新日期：1991-07-01 00:00:00
abstract：:The extracorporeal vasculature of the colonial ascidian Botryllus schlosseri plays a key role in several biological processes: transporting blood, angiogenesis, regeneration, self-nonself recognition, and parabiosis. The vasculature also interconnects all individuals in a colony and is composed of a single layer of ec...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2018.10.013
更新日期：2019-04-15 00:00:00
abstract：:Closure of the cranial neural tube depends on normal development of the head mesenchyme. Homozygous-mutant embryos for the ENU-induced open mind (opm) mutation exhibit exencephaly associated with defects in head mesenchyme development and dorsal-lateral hinge point formation. The head mesenchyme in opm mutant embryos ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.03.018
更新日期：2007-06-01 00:00:00
abstract：:Ephrins and semaphorins regulate a wide variety of developmental processes, including axon guidance and cell migration. We have studied the roles of the ephrin EFN-4 and the semaphorin MAB-20 in patterning cell-cell contacts among the cells that give rise to the ray sensory organs of Caenorhabditis elegans. In wild-ty...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00129-x
更新日期：2003-04-15 00:00:00
abstract：:Neonatal mouse hearts fully regenerate after ventricular resection similar to adult zebrafish. We established cryoinjury models to determine if different types and varying degrees of severity in cardiac injuries trigger different responses in neonatal mouse hearts. In contrast to ventricular resection, neonatal mouse ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.12.018
更新日期：2015-03-01 00:00:00
abstract：:Polychaete annelids and arthropods are both segmented protostome invertebrates. To investigate whether the segmented body plan of these two phyla share a common molecular ground pattern, we report the developmental expression of orthologues of the arthropod segment polarity genes engrailed (en), hedgehog (hh), and win...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.10.025
更新日期：2006-01-01 00:00:00
abstract：:The expression of heat shock genes is induced in all living cells by a series of proteotoxic treatments. Heat shock genes are also activated spontaneously during different phases of embryonic development. HSP89 alpha and HSC70 are expressed at a high level in the mouse blastocyst. A family of factors, called HSFs, are...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1361
更新日期：1994-12-01 00:00:00
abstract：:Auxin is implicated throughout plant growth and development. Although the effects of this plant hormone have been recognized for more than a century, it is only in the past two decades that light has been shed on the molecular mechanisms that regulate auxin homeostasis, signaling, transport, crosstalk with other hormo...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2012.05.039
更新日期：2012-09-01 00:00:00
abstract：:Mutations at the murine steel (Sl) locus encoding the ligand for the c-kit receptor result in defects in gametogenesis, hematopoiesis, and melanogenesis. Steel Panda (Slpan) is an allele at the Sl locus obtained by an X-ray mutagenesis protocol. Slpan/Slpan homozygotes are mildly anemic black-eyed whites with pigmente...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1115
更新日期：1993-05-01 00:00:00
abstract：:We have performed a detailed analysis of the expression pattern of the three gnathostome Otx classes in order to gain new insights into their functional evolution. Expression patterns were examined in the developing eye of a chondrichthyan, the dogfish, and an amniote, the chick, and compared with the capacity of para...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.11.019
更新日期：2005-02-15 00:00:00
abstract：:In marine teleosts, the aqp1ab water channel plays a vital role in the development of the pelagic egg phenotype. However, the developmental control of aqp1ab activation during oogenesis remains to be established. Here, we report the isolation of the 5'-flanking region of the teleost gilthead seabream aqp1ab gene, in w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.03.001
更新日期：2013-05-15 00:00:00
