Abstract:
:Gr16 (chromosome 2, 6.9 cM) is a dominant, grey egg mutation of the domestic silkmoth, Bombyx mori, which produces an opaque instead of a translucent chorion. To determine the underlying cause of this defect, we examined the effects of the mutation on chorion ultrastructure, protein accumulation, and biosynthesis patterns, and on the restriction patterns of early chorion structural genes, using the presumed wild-type progenitor strain, Old European 16 (OE16), for comparison. Cross-sections of mature chorions examined by scanning electron microscopy showed that the inner lamellae were oriented perpendicular, rather than parallel, to the egg surface, with a smaller vertical zone bordered by normally oriented lamellae in the heterozygote, suggestive of codominant expression. This defect first appeared in transmission electron micrographs early in choriogenesis, probably during framework formation and/or expansion stages. Mature chorion protein patterns resolved by isoelectric focusing or two-dimensional polyacrylamide gel electrophoresis, synthetic profiles of virtually all chorion proteins following pulse-labeling with tritiated or [14C]glycine, and restriction patterns of early chorion genes examined by Southern blot hybridization were identical between mutant, wild-type, and heterozygote. These observations ruled out a major deletion of chorion structural genes or defects in the production and accumulation of normal chorion proteins as mechanisms underlying the mutation, unlike other putative Gr mutants mapping in its vicinity. The sole difference that we detected was a set of minor, early protein doublets of related size but differing isoelectric point, which showed reciprocal labeling intensities in Gr16 vs OE16 and were codominantly expressed. Preliminary pulse-chase experiments suggested that these proteins may be post-translationally modified forms which undergo differential processing in mutant and wild-type. Based on these observations, we postulate that the Gr16 mutation affects the primary structure of a minor, previously unidentified chorion or follicular cell component which is critical for determining lamellar orientation, and discuss models of how this might function.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Gautreau D,Zetlan SR,Mazur GD,Goldsmith MRdoi
10.1006/dbio.1993.1112subject
Has Abstractpub_date
1993-05-01 00:00:00pages
60-72issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(83)71112-7journal_volume
157pub_type
杂志文章abstract::The germinal vesicle (GV) of starfish oocytes stays just beneath the oocyte cortex at the presumptive animal pole during the long period of oogenesis. We subjected oocytes to a centrifugal force field to detach the GV from the cortex. The association between the cortex and the GV persisted and withstood a small amount...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9577
更新日期:2000-02-15 00:00:00
abstract::The Egr family of zinc-finger transcription factors, consisting of Egr1, Egr2, Egr3, and Egr4, are involved in cellular growth and differentiation. Adult Egr3-deficient mice are ataxic and lack muscle spindle proprioceptors that normally develop at the sites of Ia afferent-myotube contacts during embryogenesis. To res...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0202
更新日期:2001-04-15 00:00:00
abstract::Homeotic genes in the Antennapedia Complex of Drosophila specify identity of the posterior head segments; the labial segment requires Sex combs reduced (Scr) for proper development, Deformed (Dfd) specifies maxillary and mandibular identity, and labial is necessary for intercalary segment identity. Although mutations ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0290
更新日期:1996-11-25 00:00:00
abstract::Drosophila nemo (nmo) and other Nemo-like kinase family members (Nlks) are well-established key regulators of numerous conserved signaling pathways, such as Wg and BMP. nmo mutants display pleiotropic defects at different developmental stages, including the embryo. In this study we describe a detailed characterization...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.04.008
更新日期:2010-07-01 00:00:00
abstract::Two 80- to 100-kb chromosomal replicons containing clustered chorion genes amplify in the ovarian follicle cells during the final 22 hr of Drosophila oogenesis. We have studied the relationship between amplification and transcription within one of these domains, located at 7E10-7F3,4 on the X chromosome. A tandem clus...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90372-6
更新日期:1986-09-01 00:00:00
abstract::Immunocytochemical studies were performed to determine if denervated rodent iris produces nerve growth factor (NGF) in a form chemically similar to that of the 7S NGF complex in mouse submandibular glands. Antisera to the alpha, beta, and gamma subunits of 7S NGF were raised in rabbits and characterized on immunoblots...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90201-0
更新日期:1986-04-01 00:00:00
abstract::Acquisition of limb-forming ability by discrete regions of the lateral plate of the chick embryo is thought to depend on a signaling cascade moving sequentially from the area of Hensen's node to the somitic mesoderm, the intermediate mesoderm, and then to the prospective limb-forming regions of the lateral plate (Step...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8646
更新日期:1997-08-15 00:00:00
abstract::The transition from maternal to zygotic gene expression in the mouse occurs in the 2-cell embryo. Previous studies in which DNA was injected into 2-cell embryos revealed that transcription promoters and origins of DNA replication are strongly repressed in cleavage stage embryos unless linked to an embryo-responsive en...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1160
更新日期:1995-06-01 00:00:00
abstract::Epidermal growth factor (EGF), a low-molecular-weight polypeptide (G. Carpenter and S. Cohen, 1979, Annu. Rev. Biochem. 48, 193-216), stimulates the proliferation and keratinisation of cultured embryonic epidermis (S. Cohen, 1965, Dev. Biol. 12, 394-407) and promotes epidermal growth, thickening, and keratinisation wh...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90243-9
更新日期:1983-12-01 00:00:00
abstract::Germ line transformation has been used to map the cis regulatory DNA elements responsible for the precise and evolutionarily stable developmental expression of the s18 chorion gene. Constructs containing chimeric combinations of Drosophila melanogaster and D. grimshawi DNA regions, as well as D. grimshawi sequences al...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90160-i
更新日期:1992-07-01 00:00:00
abstract::The Drosophila brain is tracheated by the cerebral trachea, a branch of the first segmental trachea of the embryo. During larval stages the cerebral trachea splits into several main (primary) branches that grow around the neuropile, forming a perineuropilar tracheal plexus (PNP) at the neuropile surface. Five primary ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.09.022
更新日期:2007-02-01 00:00:00
abstract::We have examined the roles of cell lineage and interactions in the determination of individual identified neurons in the grasshopper embryo by selective ablations of individual cells and/or their neighbors at successive stages following their birth. The neurons in the grasshopper central nervous system (CNS) are produ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90069-7
更新日期:1985-07-01 00:00:00
abstract::Mammalian heart development involves complex morphogenetic events which lead to the formation of fully separated left and right atrial and ventricular chambers from a tubular heart. Separation of left and right ventricular chambers is dependent on a single structure, the interventricular septum (IVS), which has both m...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.02.045
更新日期:2006-06-15 00:00:00
abstract::We wish to identify genes involved in mediating early lineage decisions in the mouse embryo. F9 teratocarcinoma cells treated with retinoic acid (RA) in suspension culture develop into embryoid bodies (EBs) with an outer layer of visceral endoderm. In order to identify genes that are involved in establishing this extr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1236
更新日期:1993-09-01 00:00:00
abstract::Pericellular proteolysis by ADAM family metalloproteinases has been widely implicated in cell signaling and development. We recently found that Xenopus ADAM13, an ADAM metalloproteinase, is required for activation of canonical Wnt signaling during cranial neural crest (CNC) induction by regulating a novel crosstalk be...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.12.031
更新日期:2012-03-01 00:00:00
abstract::We have characterized a phosphoprotein protein with a death effector domain that has a novel bifunctional role in programmed cell death. The 15-kDa phosphoprotein enriched in astrocytes (PEA-15) inhibits Fas-mediated apoptosis and increases tumor necrosis factor receptor-1 (TNF-R1)-mediated apoptosis in the same cell ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9510
更新日期:1999-12-01 00:00:00
abstract::Salivary gland formation in the Drosophila embryo is dependent on the homeotic gene Sex combs reduced (Scr). When Scr function is missing, salivary glands do not form, and when SCR is expressed everywhere in the embryo, salivary glands form in new places. Scr is normally expressed in all the cells that form the saliva...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9560
更新日期:2000-01-15 00:00:00
abstract::The contractile proteins of skeletal muscle are often represented by families of very similar isoforms. Protein isoforms can result from the differential expression of multigene families or from multiple transcripts from a single gene via alternative splicing. In many cases the regulatory mechanisms that determine the...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/0012-1606(92)90067-q
更新日期:1992-12-01 00:00:00
abstract::Neural crest cells migrate along two pathways in the trunk: the ventral path, between the neural tube and somite, and the dorsolateral path, between the somite and overlying ectoderm. In avian embryos, ventral migration precedes dorsolateral migration by nearly 24 h, and the onset of dorsolateral migration coincides w...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8963
更新日期:1998-08-15 00:00:00
abstract::The ubiquitin ligase Smurf1 can target a handful of signaling proteins for ubiquitin-mediated proteasomal destruction or functional modification, including TGF-beta receptors, Smads, transcription factors, RhoA and MEKK2. Smurf1 was initially implicated in BMP pathway regulation in embryonic development, but its poten...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.08.009
更新日期:2006-11-15 00:00:00
abstract::The Caenorhabditis elegans EGL-17/FGF protein is involved in the gonadal signaling that guides the migrations of sex myoblasts (SMs). egl-17::GFP reporter constructs are expressed dynamically in vulval cell lineages. Expression in the primary vulval cells is correlated with the precise positioning of SMs. We have inve...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00033-2
更新日期:2003-05-01 00:00:00
abstract::Enhancers are flexible in terms of their location and orientation relative to the genes they regulate. However, little is known about whether the flexibility can be applied in every combination of enhancers and genes. Enhancer detection with transposable elements is a powerful method to identify enhancers in the genom...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.12.012
更新日期:2013-03-01 00:00:00
abstract::The vertebrate liver and heart arise from adjacent cell layers in the anterior lateral (AL) endoderm and mesoderm of late gastrula embryos, and the earliest stages of liver and heart development are interrelated through reciprocal tissue interactions. Although classical embryological studies performed several decades ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.01.019
更新日期:2004-04-15 00:00:00
abstract::Leaf primordia, first visible as small bumps, are produced in a cyclical pattern at the edges of the shoot apex, a smooth region at the top of the stem. Their formation is a biomechanical process. This review first considers hypothetical construction mechanisms and then summarizes research that provides information ab...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/0012-1606(92)90089-y
更新日期:1992-09-01 00:00:00
abstract::We have used an in vitro model to investigate the relationship between cell proliferation, cell maturation, and intercellular junctional changes that are seen during normal liver development. Chick liver fragments of embryonic stages 21, 28, and 39 were grown in organ culture in Medium 199 with 10% bovine serum albumi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90265-8
更新日期:1983-09-01 00:00:00
abstract::Sex in the nematode Caenorhabditis elegans is normally determined by the X chromosome to autosome (X:A) ratio, with XX hermaphrodites and XO males. Previous work has shown that a set of at least four autosomal genes (her-1, tra-2, tra-3, and tra-1) is signaled by the X:A ratio and appears to act in a regulatory pathwa...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90077-0
更新日期:1984-11-01 00:00:00
abstract::alpha11beta1 integrin constitutes a recent addition to the integrin family. Here, we present the first in vivo analysis of alpha11 protein and mRNA distribution during human embryonic development. alpha11 protein and mRNA were present in various mesenchymal cells around the cartilage anlage in the developing skeleton ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0363
更新日期:2001-09-01 00:00:00
abstract::The GATA family of transcription factors are known to play multiple critical roles in vertebrate developmental processes, including erythropoiesis, endoderm formation and cardiogenesis. There have been no previous demonstrations of a functional role for any GATA family member being associated with musculoskeletal deve...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.12.001
更新日期:2008-02-15 00:00:00
abstract::The distribution of the extracellular matrix protein tenascin was studied in the developing chick visual system to determine its possible regulatory role in retinotectal development. Little tenascin was present in the retinal optic fiber layer, and the optic nerve and tract, but was abundant in the stratum opticum of ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1076
更新日期:1993-03-01 00:00:00
abstract::Bullous pemphigoid antisera and monoclonal antibodies to type VII collagen were used to localize hemidesmosomes and anchoring fibrils, respectively, in tissues of developing eyes and healing corneal wounds of New Zealand white rabbits. In the 17-day fetal rabbit eye, both antibodies colocalize to the epithelial-stroma...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90136-4
更新日期:1988-04-01 00:00:00