Abstract：
:Enhancers are flexible in terms of their location and orientation relative to the genes they regulate. However, little is known about whether the flexibility can be applied in every combination of enhancers and genes. Enhancer detection with transposable elements is a powerful method to identify enhancers in the genome and to create marker lines expressing fluorescent proteins in a tissue-specific manner. In the chordate Ciona intestinalis, this method has been established with a Tc1/mariner superfamily transposon Minos. Previously, we created the enhancer detection line E[MiTSAdTPOG]15 (E15) that specifically expresses green fluorescent protein (GFP) in the central nervous system (CNS) after metamorphosis. In this study, we identified the causal insertion site of the transgenic line. There are two genes flanking the causal insertion of the E15 line, and the genomic region around the insertion site contains the enhancers responsible for the expression in the endostyle and gut in addition to the CNS. We found that the endostyle and gut enhancers show sensitivity to the orientation of the GFP gene for their enhancer activity. Namely, the enhancers cannot enhance the expression of GFP which is inserted at the same orientation as the E15 line, while the enhancers can enhance GFP expression inserted at the opposite orientation. The CNS enhancer can enhance GFP expression in both orientations. The DNA element adjacent to the endostyle enhancer is responsible for the orientation sensitivity of the enhancer. The different sensitivity of the enhancers to the orientation of the transgene is a cause of CNS-specific GFP expression in the E15 line.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Hozumi A,Yoshida R,Horie T,Sakuma T,Yamamoto T,Sasakura Ydoi
10.1016/j.ydbio.2012.12.012subject
Has Abstractpub_date
2013-03-01 00:00:00pages
79-91issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(12)00678-1journal_volume
375pub_type
杂志文章abstract：:Migration of Anterior Visceral Endoderm (AVE) is a critical symmetry breaking event in the early post-implantation embryo development and is essential for establishing the correct body plan. Despite much effort, cellular and molecular events influencing AVE migration are only partially understood. Here, using time-lap...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.03.016
更新日期：2019-06-15 00:00:00
abstract：:Germ cells provide maternal mRNAs that are stored in the oocyte, and later translated at a specific time of development. In this context, gene regulation depends mainly on post-transcriptional mechanisms that contribute to keep maternal transcripts in a stable and translationally silent state. In recent years, small n...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2016.06.007
更新日期：2016-08-01 00:00:00
abstract：:Fibroblast growth factor (FGF) signalling has been implicated in the generation of mesoderm and neural fates in chordate embryos including ascidians and vertebrates. In Ciona, FGF9/16/20 has been implicated in both of these processes. However, in FGF9/16/20 knockdown embryos, notochord fate recovers during later devel...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.08.075
更新日期：2007-02-01 00:00:00
abstract：:Concentration of mast-cell precursors in hematopoietic tissues of mouse embryos was evaluated by a limiting dilution method. Cells from yolk sacs, livers, and bodies of (WB x C57BL/6)F1 (hereafter called WBB6F1)- +/+ embryos were injected directly into the skin of adult WBB6F1-W/Wv mice which were genetically depleted...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90066-0
更新日期：1983-05-01 00:00:00
abstract：:ragged seedling2 (rgd2) is a novel, recessive mutation affecting lateral organ development in maize. The mutant phenotype of homozygous rgd2-R leaves is variable. Mild leaf phenotypes have a reduced midrib and may be moderately narrow and furcated; severe Rgd2-R(-) leaves are filamentous or even radial. Despite their ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.03.027
更新日期：2005-06-15 00:00:00
abstract：:The ability to express exogenous reporter genes in intact, externally developing embryos, such as Xenopus, is a powerful tool for characterizing the activity of cis-regulatory gene elements during development. Although methods exist for generating transgenic Xenopus lines, more simplified methods for use with F0 anima...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.11.010
更新日期：2015-02-01 00:00:00
abstract：:This study was undertaken to determine whether premigratory neural crest cells of the axolotl embryo differentiate autonomously into chromatophores, or whether stimuli from the environment, particularly from the extracellular matrix, are required for this process. Neural crest cells were excised from the dorsal part o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90168-5
更新日期：1986-02-01 00:00:00
abstract：:We have tested the role of proteoglycans in the development of feather pattern by culturing 7-day-old embryonic chick skins on medium containing para-nitrophenyl-beta-D-xyloside (2 mM). Xylosides compete with core proteins of proteoglycans by acting as exogenous acceptors for the synthesis of glycosaminoglycans leadin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90199-6
更新日期：1988-05-01 00:00:00
abstract：:Patterning of the early neural tube is achieved in part by the inductive signals, which arise from neuroepithelial signaling centers. The zona limitans intrathalamica (ZLI) is a neuroepithelial domain in the alar plate of the diencephalon which separates the prethalamus from the thalamus. The ZLI has recently been con...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.05.031
更新日期：2005-08-15 00:00:00
abstract：:Hox genes are crucial players in the generation and pattering of the vertebrate trunk and posterior body during embryogenesis. Their initial expression takes place shortly after the establishment of the primitive streak, in the posterior-most part of the mouse embryo and is a determinant step for setting up the defini...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2017.02.009
更新日期：2017-08-15 00:00:00
abstract：:To identify guanine nucleotide binding proteins (G-proteins) in sea urchin eggs and to investigate their role in signal transduction at fertilization, we used cholera toxin (CTX) and pertussis toxin (PTX), which catalyze the specific ADP-ribosylation of G-proteins. Cell surface complex, consisting of plasma membranes ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90260-0
更新日期：1987-04-01 00:00:00
abstract：:The germ-line cells of many animals possess a characteristic cytoplasmic structure termed nuage or germinal granules. In mice, nuage that is prominent in postnatal male germ cells is also called intermitochondrial cement or chromatoid bodies. TDRD1/MTR-1, which contains Tudor domain repeats, is a specific component of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.10.046
更新日期：2007-01-01 00:00:00
abstract：:Summary Sentence: Conditional ablation of AP-2 gamma results in a delay in skin development and abnormal expression of p63, K14, K1, filaggrin, repetin and secreted Ly6/Plaur domain containing 1, key genes required for epidermal development and differentiation. The development of the epidermis, a stratified squamous e...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.02.017
更新日期：2008-05-01 00:00:00
abstract：:The fertilization potential in Phallusia mammillata consisted of an initial rapid depolarization. This initial sperm-triggered depolarization was followed by a phase of membrane depolarization which was of either long or short duration, depending on the eggs. When of long duration, the phase of membrane depolarization...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90108-s
更新日期：1992-10-01 00:00:00
abstract：:The scalloped and vestigial genes are both required for the formation of the Drosophila wing, and recent studies have indicated that they can function as a heterodimeric complex to regulate the expression of downstream target genes. We have analyzed the consequences of complete loss of scalloped function, ectopic expr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9939
更新日期：2000-12-15 00:00:00
abstract：:We have investigated the function of the neurogenic gene Notch (N) during development of the adult sensilla of Drosophila. Heat pulses were applied to flies carrying the temperature-sensitive Notch allele Nts1 at different larval and pupal stages. We can show that the reduction of Notch+ function during a short interv...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90147-b
更新日期：1990-11-01 00:00:00
abstract：:The early mouse embryo undertakes two types of cell division: symmetric that gives rise to the trophectoderm and then placenta or asymmetric that gives rise to inner cells that generate the embryo proper. Although cell division orientation is important, the mechanism regulating it has remained unclear. Here, we identi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.05.009
更新日期：2014-08-15 00:00:00
abstract：:The C. elegans Hox gene egl-5 (ortholog of Drosophila Abdominal-B) is expressed in multiple tissues in the tail region and is involved in tail patterning. In this study, we identify and clone the corresponding C. elegans orthologs of the components of the heterotrimeric transcription factor NFY, nfya-1, nfyb-1 and nfy...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.05.021
更新日期：2007-08-15 00:00:00
abstract：:Hippo (Hpo) signaling plays a critical role in restricting tissue growth and organ size in both invertebrate and vertebrate animals. However, how the Hpo kinase is regulated during development has not been clearly understood. Using a Bimolecular Fluorescence Complementation assay, we have investigated the functional s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.12.017
更新日期：2013-03-15 00:00:00
abstract：:Suppression of wing fate and specification of haltere fate in Drosophila by the homeotic gene Ultrabithorax is a classical example of Hox regulation of serial homology (Lewis, E.B. 1978. Nature 276, 565-570) and has served as a paradigm for understanding homeotic gene function. We have used DNA microarray analyses to ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.12.022
更新日期：2006-03-15 00:00:00
abstract：:Ephrins and semaphorins regulate a wide variety of developmental processes, including axon guidance and cell migration. We have studied the roles of the ephrin EFN-4 and the semaphorin MAB-20 in patterning cell-cell contacts among the cells that give rise to the ray sensory organs of Caenorhabditis elegans. In wild-ty...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00129-x
更新日期：2003-04-15 00:00:00
abstract：:The Hippo tumor suppressor pathway plays many fundamental cell biological roles during animal development. Two central players in controlling Hippo-dependent gene expression are the TEAD transcription factor Scalloped (Sd) and its transcriptional co-activator Yorkie (Yki). Hippo signaling phosphorylates Yki, thereby b...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.06.022
更新日期：2019-11-01 00:00:00
abstract：:Unidirectional blue light directs the rhizoid-thallus axis in the apolar zygotes of Fucus and Pelvetia. Here, it is shown that blue light (but not red light) increased cyclic GMP levels of Pelvetia zygotes by about a factor of 2. When the increase in cyclic GMP was blocked by a guanylyl cyclase inhibitor, photopolariz...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8600
更新日期：1997-07-01 00:00:00
abstract：:Epithelial sheet movement is an essential morphogenetic process during mouse embryonic eyelid closure in which Mitogen-Activated Protein 3 Kinase 1 (MAP3K1) and c-Jun play a critical role. Here we show that MAP3K1 associates with the cytoskeleton, activates Jun N-terminal kinase (JNK) and actin polymerization, and pro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.09.001
更新日期：2014-11-01 00:00:00
abstract：:The mouse prostate gland develops by branching morphogenesis from the urogenital epithelium and mesenchyme. Androgens and developmental factors, including FGF10 and SHH, promote prostate growth (Berman, D.M., Desai, N., Wang, X., Karhadkar, S.S., Reynon, M., Abate-Shen, C., Beachy, P.A., Shen, M.M., 2004. Roles for He...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.08.018
更新日期：2005-12-15 00:00:00
abstract：:Previous studies in sea urchin embryos have demonstrated that nuclearization of beta-catenin is essential for initial steps in the specification of endoderm and mesenchyme, which are derived from vegetal blastomeres. This process begins at the 4th and extends through the 9th cleavage stage, an interval in which the Sp...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00331-2
更新日期：2003-09-15 00:00:00
abstract：:We have cloned a Caenorhabditis elegans homologue of the Drosophila gap gene hunchback (hb) and have designated it hbl-1 (hunchback-like). hbl-1 encodes a predicted 982-amino-acid protein, containing two putative zinc-finger domains similar to those of Drosophila Hunchback. The gene is transcribed embryonically, but u...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9096
更新日期：1999-01-15 00:00:00
abstract：:In tetrapods, the anteroposterior (AP) patterning of the limb is under the control of the antagonistic activities of the secreted factor Sonic hedgehog (Shh) and Gli3R, the truncated repressor form of the transcription factor Gli3. In this report, we show that Msx1 and Msx2 are targets and downstream effectors of Gli3...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.04.038
更新日期：2009-07-15 00:00:00
abstract：:The Hedgehog (Hh) signal transduction pathway plays a central role in the development of invertebrates and vertebrates. While much is known about the pathway, the role of Suppressor of fused (Su(fu)), a component of the pathway's signaling complex has remained enigmatic. Previous studies have linked Su(fu) to the cyto...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.02.050
更新日期：2006-06-01 00:00:00
abstract：:Developing vertebrate limbs are often utilized as a model for studying pattern formation and morphogenetic cell death. Herein, we report that conditional deletion of Rac1, a member of the Rho family of proteins, in mouse limb bud mesenchyme led to skeletal deformities in the autopod and soft tissue syndactyly, with th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.09.014
更新日期：2009-11-15 00:00:00
