当前位置： SCI文献检索 > DEVELOPMENTAL BIOLOGY期刊下所有文献 > A cholera toxin-sensitive G-protein stimulates exocytosis in sea urchin eggs.

A cholera toxin-sensitive G-protein stimulates exocytosis in sea urchin eggs.

Abstract：

:To identify guanine nucleotide binding proteins (G-proteins) in sea urchin eggs and to investigate their role in signal transduction at fertilization, we used cholera toxin (CTX) and pertussis toxin (PTX), which catalyze the specific ADP-ribosylation of G-proteins. Cell surface complex, consisting of plasma membranes and adhering cortical vesicles, was prepared from eggs of Lytechinus variegatus and incubated with 32P-labeled NAD in the presence of CTX or PTX. CTX catalyzed the ADP-ribosylation of a 47-kDa polypeptide, whereas PTX catalyzed the ADP-ribosylation of a 40-kDa polypeptide. Microinjection of approximately 30 micrograms/ml whole CTX or approximately 20 micrograms/ml CTX subunit A into intact eggs caused exocytosis of cortical vesicles. However, if the eggs were first injected with EGTA (0.6-1.4 mM), injection of CTX did not cause exocytosis. Eggs injected with 0.8-2.8 mM cAMP or 1.0-4.0 mM adenosine 3':5'-monophosphotioate cyclic Sp-isomer (cAMP-S), a hydrolysis-resistant analog of cAMP, did not undergo exocytosis. These results suggest that a CTX-sensitive G-protein is involved in regulating Ca2+ release and exocytosis of cortical vesicles in sea urchin eggs.

journal_name

Dev Biol

journal_title

Developmental biology

authors

Turner PR,Jaffe LA,Primakoff P

doi

10.1016/0012-1606(87)90260-0

subject

Has Abstract

pub_date

1987-04-01 00:00:00

pages

577-83

issue

2

eissn

0012-1606

issn

1095-564X

pii

0012-1606(87)90260-0

journal_volume

120

pub_type

杂志文章

相关文献

DEVELOPMENTAL BIOLOGY文献大全
  • Confocal and video imaging of cytoskeleton dynamics in the leech zygote.

    abstract：:Ooplasmic segregation in the late interphase zygote of the leech Theromyzon trizonare is accomplished by reorganization of an ectoplasmic cytoskeleton formed by polar rings and meridional bands. The dynamic properties of this cytoskeleton were explored by time-lapse confocal and video microscopy. Cytoskeleton assembly...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2004.03.023

    authors： Fernández J,Toro J,Ubilla A

    更新日期：2004-07-01 00:00:00

  • Mechanisms of mesendoderm internalization in the Xenopus gastrula: lessons from the ventral side.

    abstract：:Two main processes are involved in driving ventral mesendoderm internalization in the Xenopus gastrula. First, vegetal rotation, an active movement of the vegetal cell mass, initiates gastrulation by rolling the peripheral blastocoel floor against the blastocoel roof. In this way, the leading edge of the internalized ...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1006/dbio.2001.0459

    authors： Ibrahim H,Winklbauer R

    更新日期：2001-12-01 00:00:00

  • Developmentally regulated alternative splicing of Drosophila myosin heavy chain transcripts: in vivo analysis of an unusual 3' splice site.

    abstract：:The 3' penultimate exon (exon 18) of transcripts from the muscle myosin heavy chain (MHC) gene of Drosophila melanogaster is excluded from mRNAs of embryonic and larval muscle, while it is included in mRNAs of adult thoracic muscles. By transforming organisms with the MHC gene 5' end, linked in-frame to the MHC gene 3...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(91)90235-u

    authors： Hess NK,Bernstein SI

    更新日期：1991-08-01 00:00:00

  • Fertilization triggers localized activation of Src-family protein kinases in the zebrafish egg.

    abstract：:Fertilization triggers activation of Src-family kinases in eggs of various species including marine invertebrates and lower vertebrates. While immunofluorescence studies have localized Src-family kinases to the plasma membrane or cortical cytoplasm, no information is available regarding the extent to which these kinas...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2006.03.041

    authors： Sharma D,Kinsey WH

    更新日期：2006-07-15 00:00:00

  • The effect of back-transplants of the embryonic gut wall on growth of the neural tube.

    abstract：:Experiments in which the developing gut of avian embryos was back-transplanted to permit the bowel to interact with the developing neural tube were undertaken. Segments of intestine from 4-day quail embryos were implanted between the somites and neural tubes of chick embryos of 7 to 24 somites. The spinal cord respond...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(87)90486-6

    authors： Rothman TP,Gershon MD,Fontaine-Pérus JC,Chanconie M,Le Douarin NM

    更新日期：1987-12-01 00:00:00

  • Microinjection of lectins, hyaluronidase, and hyaluronate fragments interferes with cleavage delay and mesoderm induction in embryos of Patella vulgata.

    abstract：:In embryos of Patella vulgata at the 32-cell stage, one of the four vegetally located macromeres makes contacts with overlying animal micromeres. As a result, this macromere (designated 3D) divides significantly later than the other macromeres and forms the mesodermal stem cell 4d. Shortly before and during this inter...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(89)90239-x

    authors： Kühtreiber WM,van Dongen CA

    更新日期：1989-04-01 00:00:00

  • Dynamics of dendritic spines and their afferent terminals: spines are more motile than presynaptic boutons.

    abstract：:Previous work has established that dendritic spines, sites of excitatory input in CNS neurons, can be highly dynamic, in later development as well as in mature brain. Although spine motility has been proposed to facilitate the formation of new synaptic contacts, we have reported that spines continue to be dynamic even...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2004.09.028

    authors： Deng J,Dunaevsky A

    更新日期：2005-01-15 00:00:00

  • Gene regulation by the lysine demethylase KDM4A in Drosophila.

    abstract：:Lysine methylation of histones is associated with both transcriptionally active chromatin and with silent chromatin, depending on what residue is modified. Histone methyltransferases and demethylases ensure that histone methylations are dynamic and can vary depending on cell cycle- or developmental stage. KDM4A demeth...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2012.11.011

    authors： Crona F,Dahlberg O,Lundberg LE,Larsson J,Mannervik M

    更新日期：2013-01-15 00:00:00

  • Early amphibian (anuran) morphogenesis is sensitive to novel gravitational fields.

    abstract：:Anuran amphibian embryos (Xenopus laevis and Rana dybowskii) are sensitive to novel gravitational fields. Under simulated weightlessness, (i) the location of the first horizontal cleavage furrow was shifted toward the vegetal pole at the eight-cell stage; (ii) the position of the blastocoel was more centered, and the ...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1006/dbio.1993.1024

    authors： Neff AW,Yokota H,Chung HM,Wakahara M,Malacinski GM

    更新日期：1993-01-01 00:00:00

  • Genetic patterning in the adult capitate antenna of the beetle Tribolium castaneum.

    abstract：:Antenna structure varies widely among insects, in contrast to the well-conserved structure of legs. The adult capitate antenna of the red flour beetle, Tribolium castaneum, is composed of eleven articles, organized into four distinct morphological regions (scape, pedicel, funicle and club). Here, we report the use of ...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2008.10.047

    authors： Angelini DR,Kikuchi M,Jockusch EL

    更新日期：2009-03-01 00:00:00

  • Myf5 and MyoD activation define independent myogenic compartments during embryonic development.

    abstract：:Gene targeting has indicated that Myf5 and MyoD are required for myogenic determination because skeletal myoblasts and myofibers are missing in mouse embryos lacking both Myf5 and MyoD. To investigate the fate of Myf5:MyoD-deficient myogenic precursor cells during embryogenesis, we examined the sites of epaxial, hypax...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/s0012-1606(03)00139-8

    authors： Kablar B,Krastel K,Tajbakhsh S,Rudnicki MA

    更新日期：2003-06-15 00:00:00

  • Biophysical mechanisms for morphogenetic progressions at the shoot apex.

    abstract：:Leaf primordia, first visible as small bumps, are produced in a cyclical pattern at the edges of the shoot apex, a smooth region at the top of the stem. Their formation is a biomechanical process. This review first considers hypothetical construction mechanisms and then summarizes research that provides information ab...

    journal_title：Developmental biology

    pub_type： 杂志文章,评审

    doi：10.1016/0012-1606(92)90089-y

    authors： Selker JM,Steucek GL,Green PB

    更新日期：1992-09-01 00:00:00

  • Chicken Ovalbumin Upstream Promoter-Transcription Factor II (COUP-TFII) regulates growth and patterning of the postnatal mouse cerebellum.

    abstract：:COUP-TFII (also known as Nr2f2), a member of the nuclear orphan receptor superfamily, is expressed in several regions of the central nervous system (CNS), including the ventral thalamus, hypothalamus, midbrain, pons, and spinal cord. To address the function of COUP-TFII in the CNS, we generated conditional COUP-TFII k...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2008.11.001

    authors： Kim BJ,Takamoto N,Yan J,Tsai SY,Tsai MJ

    更新日期：2009-02-15 00:00:00

  • Functional equivalence and rescue among group 11 Hox gene products in vertebral patterning.

    abstract：:Hoxa-11 and Hoxd-11 are paralogous genes required for proper development of the vertebral column, the limbs, and the urogenital system. To further explore the functional relationship between these genes, as well as the potential rescue of one function by the other, we have introduced a Hoxd-11-expressing transgene int...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1006/dbio.1996.0137

    authors： Zákány J,Gérard M,Favier B,Potter SS,Duboule D

    更新日期：1996-06-15 00:00:00

  • Distinct roles for retinoic acid receptors alpha and beta in early lung morphogenesis.

    abstract：:Retinoic acid (RA) signaling is required for normal development of multiple organs. However, little is known about how RA influences the initial stages of lung development. Here, we used a combination of genetic, pharmacological and explant culture approaches to address this issue, and to investigate how signaling by ...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2005.10.045

    authors： Desai TJ,Chen F,Lü J,Qian J,Niederreither K,Dollé P,Chambon P,Cardoso WV

    更新日期：2006-03-01 00:00:00

  • Role of the amnioserosa in germ band retraction of the Drosophila melanogaster embryo.

    abstract：:As the germ band shortens in Drosophila melanogaster embryos, cell shape changes cause segments to narrow anteroposteriorly and to lengthen dorsoventrally. One of the genes required for this retraction process is the hindsight (hnt) gene. hnt encodes a nuclear Zinc-finger protein that is expressed in the extraembryoni...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1006/dbio.1999.9409

    authors： Lamka ML,Lipshitz HD

    更新日期：1999-10-01 00:00:00

  • BMP signaling and stem cell regulation.

    abstract：:Stem cells play an essential role in cellular specialization and pattern formation during embryogenesis and in tissue regeneration in adults. This is mainly due to a stem cell's ability to replenish itself (self-renewal) and, at the same time, produce differentiated progeny. Realization of these special stem cell feat...

    journal_title：Developmental biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ydbio.2005.05.009

    authors： Zhang J,Li L

    更新日期：2005-08-01 00:00:00

  • The oblique muscle organizer in Hirudo medicinalis, an identified embryonic cell projecting multiple parallel growth cones in an orderly array.

    abstract：:The oblique muscle layer in the leech body wall is built upon the processes of a unique identified embryonic cell, the Comb- or C-cell. Each C-cell is composed of a spindle-shaped soma that projects approximately 70 parallel processes through the developing body wall at an angle oblique to the long axis. The morphogen...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(91)90342-z

    authors： Jellies J,Kristan WB Jr

    更新日期：1991-11-01 00:00:00

  • Roles of growth hormone and insulin-like growth factor 1 in mouse postnatal growth.

    abstract：:To examine the relationship between growth hormone (GH) and insulin-like growth factor 1 (IGF1) in controlling postnatal growth, we performed a comparative analysis of dwarfing phenotypes manifested in mouse mutants lacking GH receptor, IGF1, or both. This genetic study has provided conclusive evidence demonstrating t...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1006/dbio.2000.9975

    authors： Lupu F,Terwilliger JD,Lee K,Segre GV,Efstratiadis A

    更新日期：2001-01-01 00:00:00

  • Mechanisms of gonadal morphogenesis are not conserved between chick and mouse.

    abstract：:To understand mechanisms of sex determination, it is important to know the lineage relationships of cells comprising the gonads. For example, in mice, the Y-linked gene Sry triggers differentiation of Sertoli cells from a cell population originating in the coelomic epithelium overlying the nascent gonad that also give...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2006.09.007

    authors： Sekido R,Lovell-Badge R

    更新日期：2007-02-01 00:00:00

  • The patterns on chondrogenesis of cells from facial primordia of chick embryos in micromass culture.

    abstract：:Chondrogenesis of mesenchymal cells from the frontonasal mass, mandibles and maxillae of stage-24 chick embryos has been investigated in micromass (high-density) cultures. Distinct differences in the amount and pattern of cartilage differentiation are found. In cultures of frontonasal mass cells, a central sheet of ca...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(86)90349-0

    authors： Wedden SE,Lewin-Smith MR,Tickle C

    更新日期：1986-09-01 00:00:00

  • Analysis of mouse kreisler mutants reveals new roles of hindbrain-derived signals in the establishment of the otic neurogenic domain.

    abstract：:The inner ear, the sensory organ responsible for hearing and balance, contains specialized sensory and non-sensory epithelia arranged in a highly complex three-dimensional structure. To achieve this complexity, a tight coordination between morphogenesis and cell fate specification is essential during otic development....

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2008.07.025

    authors： Vázquez-Echeverría C,Dominguez-Frutos E,Charnay P,Schimmang T,Pujades C

    更新日期：2008-10-01 00:00:00

  • Binding of both acrosome-intact and acrosome-reacted guinea pig sperm to the zona pellucida during in vitro fertilization.

    abstract：:Mammalian sperm-egg adhesion occurs when sperm bind to the zona pellucida of the egg. In this study with guinea pig gametes, we have asked if sperm can initiate binding to the zona before and after the acrosome reaction and if the sperm surface protein PH-20 is involved in the binding at these two stages. Sperm bindin...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(87)90191-6

    authors： Myles DG,Hyatt H,Primakoff P

    更新日期：1987-06-01 00:00:00

  • Contacts between the commissural axons and the floor plate cells are mediated by nectins.

    abstract：:During development of the central nervous system (CNS), commissural axons grow toward the ventral midline. After crossing the floor plate, they abruptly change their trajectory from the circumferential to the longitudinal axis. The contacts between the commissural axons and the floor plate cells are involved in this a...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2004.05.034

    authors： Okabe N,Shimizu K,Ozaki-Kuroda K,Nakanishi H,Morimoto K,Takeuchi M,Katsumaru H,Murakami F,Takai Y

    更新日期：2004-09-15 00:00:00

  • Patterns of maternal messenger RNA accumulation and adenylation during oogenesis in Urechis caupo.

    abstract：:We have investigated the accumulation and adenylation of the maternal mRNA during oogenesis in the oocytes of the marine worm Urechis caupo. The analysis, using in vitro translation and cDNA probes to assay for specific mRNAs, demonstrates that different maternal mRNAs accumulate with different patterns during oogenes...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(86)90347-7

    authors： Rosenthal ET,Wilt FH

    更新日期：1986-09-01 00:00:00

  • EAST and Chromator control the destruction and remodeling of muscles during Drosophila metamorphosis.

    abstract：:Metamorphosis involves the destruction of larval, the formation of adult and the transformation of larval into adult tissues. In this study, we demonstrate the role of the Drosophila nuclear proteins EAST and Chromator in tissue destruction and remodeling. To better understand the function of east, we performed a yeas...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/j.ydbio.2007.05.001

    authors： Wasser M,Bte Osman Z,Chia W

    更新日期：2007-07-15 00:00:00

  • Growth regulation of the interstitial cell population in hydra. I. Evidence for global control by nerve cells in the head.

    abstract：:The interstitial cells of hydra form a multipotent stem cell system, producing terminally differentiated nerve cells and nematocytes during asexual growth. Under well-fed conditions the interstitial cell population doubles in size every 4 days. We have investigated the possible role of nerve cells in regulating this b...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(85)90089-2

    authors： Heimfeld S,Bode HR

    更新日期：1985-08-01 00:00:00

  • A gradient of gap junctional communication along the anterior-posterior axis of the developing chick limb bud.

    abstract：:A modification of the scrape-loading/dye transfer technique was used to study gap junctional communication along the anterior-posterior (A-P) axis of embryonic chick wing buds at an early stage of development (stage 20/21) when positional values along the A-P axis are being specified. Extensive intercellular transfer ...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(91)90271-4

    authors： Coelho CN,Kosher RA

    更新日期：1991-12-01 00:00:00

  • An antiserum (ES1) against a particulate form of extracellular matrix blocks the transition of cardiac endothelium into mesenchyme in culture.

    abstract：:The epithelial-mesenchymal transition of cardiac endothelium is a critical developmental event in the formation of valvular and septal anlagen. We have demonstrated previously that this event can be mimicked in culture by treating atrioventricular canal (AV) endothelium with EDTA-soluble proteins extracted from embryo...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1016/0012-1606(91)90121-i

    authors： Mjaatvedt CH,Krug EL,Markwald RR

    更新日期：1991-06-01 00:00:00

  • The cloche and spadetail genes differentially affect hematopoiesis and vasculogenesis.

    abstract：:In vertebrates, hematopoietic and vascular progenitors develop from ventral mesoderm. The first primitive wave of hematopoiesis yields embryonic red blood cells, whereas progenitor cells of subsequent definitive waves form all hematopoietic cell lineages. In this report we examine the development of hematopoietic and ...

    journal_title：Developmental biology

    pub_type： 杂志文章

    doi：10.1006/dbio.1998.8887

    authors： Thompson MA,Ransom DG,Pratt SJ,MacLennan H,Kieran MW,Detrich HW 3rd,Vail B,Huber TL,Paw B,Brownlie AJ,Oates AC,Fritz A,Gates MA,Amores A,Bahary N,Talbot WS,Her H,Beier DR,Postlethwait JH,Zon LI

    更新日期：1998-05-15 00:00:00