Abstract：
:Ooplasmic segregation in the late interphase zygote of the leech Theromyzon trizonare is accomplished by reorganization of an ectoplasmic cytoskeleton formed by polar rings and meridional bands. The dynamic properties of this cytoskeleton were explored by time-lapse confocal and video microscopy. Cytoskeleton assembly was investigated in zygotes pulse-labeled with microinjected fluorophore-tagged or biotin-tagged dimeric tubulin and G-actin. Cytoskeleton disassembly was studied by comparing the linear dimensions of the cytoskeleton at different time points during late interphase. The relative distributions of F- and-G-actin were determined after microinjection of rhodamine-labeled actin and fluorescein-labeled DNase I. Results showed that labeled precursors were readily incorporated into a network of microtubules or actin filaments. Bipolar translocation of the rings and meridional bands was accompanied by the rapid assembly and disassembly of microtubules and actin filaments. Because labeled microtubules and microfilaments gradually decreased, the rate of cytoskeleton disassembly was greater than the rate of cytoskeleton assembly. Hence, ooplasmic segregation was accompanied by the rapid turnover of cytoskeletal components. Co-distribution of F- and-G-actin during mid and late interphase may favor polymer-monomer interchange. We conclude that cytoskeleton reorganization during foundation of cytoplasmic domains can be conveniently studied in the live leech zygote after microinjection of labeled precursors.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Fernández J,Toro J,Ubilla Adoi
10.1016/j.ydbio.2004.03.023subject
Has Abstractpub_date
2004-07-01 00:00:00pages
59-74issue
1eissn
0012-1606issn
1095-564Xpii
S001216060400212Xjournal_volume
271pub_type
杂志文章abstract：:The role of angiotensin II (Ang II) in the early embryonic development of the heart has not been examined. We have used RT-PCR to identify mRNA for angiotensinogen, angiotensin-converting enzyme, and the Ang II AT1 and AT2 receptors in embryonic day 10.25 Sprague-Dawley rats, and have used confocal microscopy to local...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8764
更新日期：1997-12-15 00:00:00
abstract：:Oligodendrocyte precursor cells (OPCs) can be differentiated in culture into either oligodendrocytes or type-2 astrocytes (2As), depending on the culture conditions. Whereas oligodendrocyte development can occur in the absence of inducing signals, 2A development apparently cannot. Fetal calf serum (FCS) and bone morph...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.11.013
更新日期：2004-03-01 00:00:00
abstract：:The cornea is one of the most densely innervated structures of the body. In the developing chicken embryo, nerves from the ophthalmic trigeminal ganglion (OTG) innervate the cornea in a series of spatially and temporally regulated events. However, little is known concerning the signals that regulate these events. Here...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.04.032
更新日期：2010-08-01 00:00:00
abstract：:Developmental pattern formation allows cells within a tissue or organ to coordinate their development and establish cell types in relationship to one another. To better characterize the developmental patterning events within one organ, the C. elegans hindgut, we have analyzed the expression pattern of several genes us...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00352-x
更新日期：2003-10-01 00:00:00
abstract：:Huntington disease (HD) is an adult-onset neurodegenerative disease characterized by motor, cognitive, and psychiatric symptoms that is caused by a CAG expansion in the HTT gene. Palmitoylation is the addition of saturated fatty acids to proteins by DHHC palmitoylacyl transferases. HTT is palmitoylated by huntingtin i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.11.018
更新日期：2015-01-15 00:00:00
abstract：:BMP-2 and BMP-4 are known to be involved in the early events which specify the cardiac lineage. Their later patterns of expression in the developing mouse and chick heart, in the myocardium overlying the atrioventricular canal (AV) and outflow tract (OFT) cushions, also suggest that they may play a role in valvoseptal...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0291
更新日期：2001-07-01 00:00:00
abstract：:Clotting is critical in limiting hemolymph loss and initiating wound healing in insects as in vertebrates. It is also an important immune defense, quickly forming a secondary barrier to infection, immobilizing bacteria and thereby promoting their killing. However, hemolymph clotting is one of the least understood immu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.03.019
更新日期：2006-07-01 00:00:00
abstract：:Connective tissue outgrowths of neonatal muscle onto a substratum of bone matrix differentiate into cartilage in response to a bone morphogenetic protein (BMP). The BMP can be separated from bone matrix by extraction with 4 M guanidine hydrochloride (GuHCl) or degraded in situ by endogenous proteolytic enzymes to deac...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90339-9
更新日期：1988-12-01 00:00:00
abstract：:The evolution of canalized traits is a central question in evolutionary biology. Natural variation in highly conserved traits can provide clues about their evolutionary potential. Here we investigate natural variation in a conserved trait-even-skipped (eve) expression at the cellular blastoderm stage of embryonic deve...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.06.019
更新日期：2015-09-01 00:00:00
abstract：:Using lineage tracers, we recently showed dorsal and ventral ectodermal compartments along the sides of the body in chick embryos. The compartments are formed both in presumptive limb-forming regions where they position the apical ridge and also in presumptive interlimb (flank). Here we show, using a novel technique c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9659
更新日期：2000-06-15 00:00:00
abstract：:Implantation represents a critical step for embryonic development and pregnancy. Its success depends on the complex interplay between a receptive endometrium and a competent embryo. Implantation-related events remain hardly accessible, making implantation a true "black box" in developmental biology. Improved in vitro ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2020.04.003
更新日期：2020-07-01 00:00:00
abstract：:Cells anchor to substrates by binding to extracellular matrix (ECM). In addition to this anchoring function however, cell-ECM binding is a mechanism for cells to sense their surroundings and to communicate and coordinate behaviour amongst themselves. Several ECM molecules and their receptors play essential roles in mu...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2011.03.015
更新日期：2011-06-15 00:00:00
abstract：:Chromatin regulators contribute to the developmental control of gene expression. In the nematode Caenorhabditis elegans, the roles of chromatin regulation in development have been explored in several contexts, including vulval differentiation. The synthetic multivulva (synMuv) genes are regulators of vulval developmen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.05.018
更新日期：2014-08-15 00:00:00
abstract：:The pattern of innervation in 13 chicken hindlimb muscles was studied at various stages of development in order to examine the mechanisms which regulate its formation. The pattern of innervation was visualized by examining the distribution of fiber types within each muscle. It was found that the fiber type which a myo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90080-5
更新日期：1983-05-01 00:00:00
abstract：:Using gene-specific probes derived from four mouse gamma-crystallin cDNAs, we have examined the regulation of different members of the mouse gamma-crystallin gene family during lens development. Our analysis revealed that, while the different gamma-crystallin genes appear to be coordinately activated during embryogene...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90227-2
更新日期：1987-01-01 00:00:00
abstract：:Chick sensory neurons grow to their correct targets in the hindlimb from the outset during normal development and following various experimental manipulations. This may result not because sensory neurons respond to specific limb-derived cues, but because they interact in some way with motoneurons which are responsive ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90023-0
更新日期：1986-12-01 00:00:00
abstract：:During early vertebrate development, members of the transforming growth factor beta (TGFbeta) family play important roles in a variety of processes, including germ layer specification, patterning, cell differentiation, migration, and organogenesis. The activities of TGFbetas need to be tightly controlled to ensure the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00075-1
更新日期：2003-03-01 00:00:00
abstract：:Meckel-Gruber syndrome (MKS) is an embryonic lethal ciliopathy resulting from mutations in genes encoding proteins localising to the primary cilium. Mutations in the basal body protein MKS1 account for 7% of cases of MKS. The condition affects the development of multiple organs, including brain, kidney and skeleton. H...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.02.015
更新日期：2013-05-01 00:00:00
abstract：:The distribution of microtubules and microtubule organizing centers in the events leading up to the establishment of the first asymmetric cleavage furrow in nematode embryos was followed using indirect immunofluorescence of antibodies to tubulin. Oocytes arrest in meiotic prophase then undergo two meiotic reduction di...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90117-9
更新日期：1984-01-01 00:00:00
abstract：:Cleft palate is one of the most common craniofacial congenital defects in humans. It is associated with multiple genetic and environmental risk factors, including mutations in the genes encoding signaling molecules in the sonic hedgehog (Shh) pathway, which are risk factors for cleft palate in both humans and mice. Ho...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.07.003
更新日期：2018-09-01 00:00:00
abstract：:The elongation of embryo and tissue is a key morphogenetic event in embryogenesis and organogenesis. Notochord, a typical chordate organ, undergoes elongation to perform its regulatory roles and to form the structural support in the embryo. Notochord elongation is morphologically similar across all chordates, but asci...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2018.11.009
更新日期：2019-04-15 00:00:00
abstract：:Intercellular communication mediated by gap junctions is important for tissue homeostasis in the avascular lens, and extensive areas of gap junctions form between fiber cells during fiber cell differentiation and lens development. We examined the role of the calcium-dependent cell adhesion molecule, N-cadherin, in the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0237
更新日期：1996-10-10 00:00:00
abstract：:Soluble N-ethylmalameide-sensitive factor attachment protein receptor (SNARE) proteins are present in mammalian sperm and could be involved in critical membrane fusion events during fertilization, namely the acrosome reaction. Vesicle-associated membrane protein/synaptobrevin, a SNARE on the membrane of a vesicular ca...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9745
更新日期：2000-07-01 00:00:00
abstract：:Alkaline phosphatase (AP) activity is expressed in the endodermal cell lineage of ascidian embryos beginning at gastrulation. AP expression is resistant to levels of actinomycin D which completely suppress the appearance of other tissue-specific enzyme and morphological markers including acetylcholinesterase (AchE), a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90043-1
更新日期：1987-02-01 00:00:00
abstract：:As the germ band shortens in Drosophila melanogaster embryos, cell shape changes cause segments to narrow anteroposteriorly and to lengthen dorsoventrally. One of the genes required for this retraction process is the hindsight (hnt) gene. hnt encodes a nuclear Zinc-finger protein that is expressed in the extraembryoni...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9409
更新日期：1999-10-01 00:00:00
abstract：:A newly defined genetic locus designated lethal (1) optic ganglion reduced (l(1)ogre: 1-18.8, 6E1/2-6E4/5) is characterized. Four alleles have been isolated, one organismal viable and three organismal lethals. Histological analyses of these mutants at the light microscopic level have detected defects only in the devel...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90009-0
更新日期：1985-03-01 00:00:00
abstract：:Early embryonic cells are capable of acquiring numerous developmental fates until they become irreversibly committed to specific lineages depending on intrinsic determinants and/or regional interactions. From fertilization to gastrulation, such pluripotent cells first increase in number and then turn to undergoing dif...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2019.04.014
更新日期：2019-08-15 00:00:00
abstract：:Most metazoans are able to grow beyond a few hundred cells and to support differentiated tissues because they elaborate multicellular, epithelial tubes that are indispensable for nutrient and gas exchange. To identify and characterize the cellular behaviors and molecular mechanisms required for the morphogenesis of ep...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.10.034
更新日期：2016-01-01 00:00:00
abstract：:The survival effects of brain-derived neurotrophic factor (BDNF) on the ganglion cells of the chick retina were studied in vitro at different embryonic ages. We found these effects to be strongly age-dependent: at E5, when the first ganglion cell axons have crossed the optic chiasm, but not yet reached the tectum, gan...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90256-x
更新日期：1989-12-01 00:00:00
abstract：:In the developing retina, as in other regions of the CNS, neural progenitors give rise to individual cell types during discrete temporal windows. Pax6 is expressed in retinal progenitor cells (RPCs) throughout the course of retinogenesis, and has been shown to be required during early retinogenesis for generation of m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.09.030
更新日期：2017-12-01 00:00:00
