Abstract：
:The distribution of microtubules and microtubule organizing centers in the events leading up to the establishment of the first asymmetric cleavage furrow in nematode embryos was followed using indirect immunofluorescence of antibodies to tubulin. Oocytes arrest in meiotic prophase then undergo two meiotic reduction divisions after fertilization. At both of these divisions barrel-shaped spindles were observed. Initially a single microtubule organizing center was seen adjacent to the sperm pronucleus following fertilization in Caenorhabditis elegans, but later two sperm asters were distinguished. These increased in size as the egg pronucleus migrated toward the sperm pronucleus and reached maximum size, with fascicles of microtubules extending to the cortex, once the pronuclei had become juxtaposed. The first cleavage spindle formed following rotation and migration of the juxtaposed pronuclei back toward the center of the embryo. The distribution of microtubules in a temperature-sensitive mutant that fails in both pronuclear migration and rotation was also examined. Asters in the mutant embryos at the nonpermissive temperature contained only short microtubules suggesting that the morphology of the asters is important for directing the movement of the pronuclei. In Panagrellus redivivus sperm asters were not detected by anti-tubulin staining until the female pronucleus had migrated to the centrally placed sperm pronucleus. Asters then increased in size and formed the first cleavage spindle.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Albertson DGdoi
10.1016/0012-1606(84)90117-9subject
Has Abstractpub_date
1984-01-01 00:00:00pages
61-72issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(84)90117-9journal_volume
101pub_type
杂志文章abstract：:Pioneer axons in insect legs are experimentally accessible model systems for the molecular identification and cellular localization of guidance cues regulating the path of axon growth. A detailed study of the Fe2 pioneer axons in the legs of the cockroach was performed to examine the diversity of guidance mechanisms. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8704
更新日期：1997-10-15 00:00:00
abstract：:Division of a female Drosophila stem cell produces a daughter stem cell and a cystoblast. The cystoblast produces a syncytial cluster of 16 cells by precisely four mitotic divisions and incomplete cytokinesis. Mutations in genes required for cystoblast differentiation, such as bag-of-marbles, block syncytial cluster f...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9346
更新日期：1999-08-15 00:00:00
abstract：:Developmental patterning and growth of the vertebrate digestive and respiratory tracts requires interactions between the epithelial endoderm and adjacent mesoderm. The esophagus is a specialized structure that connects the digestive and respiratory systems and its normal development is critical for both. Shh signaling...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.08.024
更新日期：2011-11-15 00:00:00
abstract：:The spe-9 gene is required for fertility in Caenorhabditis elegans and encodes a sperm transmembrane protein with an extracellular domain (ECD) that contains 10 epidermal growth factor (EGF) repeats. Deletion analysis reveals that the EGF repeats and the transmembrane domain are required for fertilization. In contrast...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.05.014
更新日期：2004-08-15 00:00:00
abstract：:Retinoic acid (RA) is a potent inducer of cell differentiation and plays an essential role in sex-specific germ cell development in the mammalian gonad. RA is essential for male gametogenesis and hence fertility. However, RA can also disrupt sexual cell fate in somatic cells of the testis, promoting transdifferentiati...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.02.015
更新日期：2017-04-15 00:00:00
abstract：:Development of the Drosophila embryonic midgut is dependent on a number of genes, including tinman and bagpipe, which are required for formation of the visceral mesoderm, and the homeotic genes and their targets, which act locally in the visceral mesoderm to direct formation of specific midgut constrictions. Here we r...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1171
更新日期：1995-06-01 00:00:00
abstract：:Developmentally regulated gene expression has been analyzed in the wild-type D. discoideum strain NC-4 and a series of temporally deranged mutants. The mutants include representatives from each class of rapid development mutation, Fr17(rdeA-) and HT506(rdeC-), and strain HIfm-1, which appears to be defective in the ti...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90260-5
更新日期：1986-06-01 00:00:00
abstract：:Thyroid hormone deficiency is known to deeply affect cerebellum post-natal development. We present here a detailed analysis of the phenotype of a recently generated mouse model, expressing a dominant-negative TRα1 mutation. Although hormonal level is not affected, the cerebellum of these mice displays profound alterat...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.05.657
更新日期：2011-08-15 00:00:00
abstract：:Complex intrinsic and extrinsic mechanisms determine neural cell fate during development of the nervous system. Using Id4 deficient mice, we show that Id4 is required for normal development of the central nervous system (CNS), timing neural differentiation in the developing forebrain. In the absence of Id4, the ventri...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.02.001
更新日期：2005-04-15 00:00:00
abstract：:The nervous system development of the sea cucumber Stichopus japonicus was investigated to explore the development of the bilateral larval nervous system into the pentaradial adult form typical of echinoderms. The first nerve cells were detected in the apical region of epidermis in the late gastrula. In the auriculari...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.12.038
更新日期：2006-04-01 00:00:00
abstract：:We previously isolated a p53 cDNA from a Xenopus oocyte library. To determine if p53 has a function in the developmental period, we have studied its expression at the RNA and protein levels during the early development of Xenopus laevis. Two p53 transcripts (3 and 2.2 kb) are expressed from the beginning of Xenopus oo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1230
更新日期：1993-09-01 00:00:00
abstract：:In the zebrafish embryo, cells fated to give rise to the rostral brain move in a concerted fashion and retain tissue coherence during morphogenesis. We demonstrate here that Otx proteins have a dramatic effect on cell-cell interactions when expressed ectopically in the zebrafish embryo. Injection of zebrafish Otx1 or ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9771
更新日期：2000-07-15 00:00:00
abstract：:Following their birth in olfactory placode, luteinizing hormone-releasing hormone (LHRH)-containing neurons migrate across the developing cribriform plate and form a dispersed population in the mammalian basal forebrain. The present study reveals the colocalization of unique glycoconjugate antigens (detected with mono...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1045
更新日期：1993-02-01 00:00:00
abstract：:Cadherin-11 (cad-11) is a novel member of the cadherin family of cell adhesion molecules, having recently been identified by means of the polymerase chain reaction. To study the function and expression of this molecule, we cloned mouse, cad-11 cDNA. Transfection of L cells with cDNA led them to acquire a typical cadhe...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1149
更新日期：1995-05-01 00:00:00
abstract：:The correct assembly of junction components, such as E-cadherin and beta-catenin, into the zonula adherens is fundamental for the function of epithelia, both in flies and in vertebrates. In C. elegans, however, the cadherin-catenin system is not essential for general adhesion, raising the question as to the genetic ba...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.0113
更新日期：2001-02-01 00:00:00
abstract：:Genetic interactions regulating intermediate stages of tubulogenesis in the developing kidney have been difficult to define. A systems biology strategy using microarray was combined with in vitro/ex vivo and genetic approaches to identify pathways regulating specific stages of tubulogenesis. Analysis of the progressio...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.01.014
更新日期：2012-04-01 00:00:00
abstract：:Myotendinous junctions (MTJs) are specialized sites at the surface of muscle fibers where force is transmitted between muscle and tendon. Morphogenesis of MTJs in the latter half of embryonic chick development is characterized by formation of basement membrane, appearance of subsarcolemmal densities, association of my...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1161
更新日期：1994-06-01 00:00:00
abstract：:Only a subset of cleavage stage blastomeres in the Xenopus embryo is competent to contribute cells to the retina; ventral vegetal blastomeres do not form retina even when provided with neuralizing factors or transplanted to the most retinogenic position of the embryo. These results suggest that endogenous maternal fac...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.01.040
更新日期：2007-05-01 00:00:00
abstract：:Drosophila N-cadherin (CadN) is an evolutionarily conserved, atypical classical cadherin, which has a large complex extracellular domain and a catenin-binding cytoplasmic domain. We have previously shown that CadN regulates target selection of R7 photoreceptor axons. To determine the functional domains of CadN, we con...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.01.030
更新日期：2007-04-15 00:00:00
abstract：:Previous experiments in which vitamin A has been administered to developing or regenerating limbs have shown that different limb axes are affected. In regenerating axolotl limbs, serial reduplications in the proximodistal axis are produced. In the developing chick limb bud, mirror-imaged reduplications in the anteropo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90370-6
更新日期：1983-08-01 00:00:00
abstract：:Using two different models of pregnancy failure in mice, we show in this communication that preimplantation embryonic development can be controlled, in vivo, with cytokines. In one model, described by us previously, CSF-1 is shown to block gestations in plugged females due to its induction of defective early developme...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90236-v
更新日期：1991-08-01 00:00:00
abstract：:A rapid, gentle technique is described for the isolation of nuclei from sea urchin embryos. Using this technique, we have analyzed the synthesis and accumulation of nonhistone nuclear proteins during sea urchin development by two-dimensional gel electrophoresis. Most nuclear proteins fall into one of three patterns of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90445-3
更新日期：1987-09-01 00:00:00
abstract：:CREB-2 (also called ATF4, TAXREB67, or C/ATF) is an evolutionarily conserved member of the CREB/ATF family of basic-leucine zipper transcription factors. CREB-2 is expressed ubiquitously in the adult mouse and can function as both a transcriptional activator and a repressor. However, little was understood about the no...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9699
更新日期：2000-06-01 00:00:00
abstract：:Reproductive plasticity is the ability of an animal to modulate its reproductive functions in response to environmental changes. For example, Caenorhabditis elegans, a free-living nematode, can adjust the onsets of oogenesis and embryogenesis under harsh environmental conditions, including starvation. However, the mol...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.08.014
更新日期：2017-10-01 00:00:00
abstract：:This work describes unknown aspects of chordotonal organ (ChO) morphogenesis revealed in post-embryonic stages through the use of new fluorescently labeled markers. We show that towards the end of embryogenesis a hitherto unnoticed phase of cell migration commences in which the cap cells of the ventral ChOs elongate a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.01.009
更新日期：2016-03-01 00:00:00
abstract：:Type 1 protein phosphatases (PP1) are involved in diverse cellular activities, ranging from glycogen metabolism to chromatin structure modification, mitosis, and meiosis. The holoenzymes are composed of two or more subunits, including a catalytic subunit (PP1c) and one or more regulatory subunits. Many eukaryotes poss...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9100
更新日期：1999-01-01 00:00:00
abstract：:The formation and maintenance of the highly regular organization of membrane systems and proteins in striated muscle require specific membrane-membrane and membrane-cytoskeleton interactions. The development of T-tubules and sarcoplasmic reticulum (SR) was followed in gastrocnemius muscle fibers from chicken embryos b...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1292
更新日期：1993-11-01 00:00:00
abstract：:Maize leaves are divided into repeated longitudinal units consisting of vascular tissue, bundle sheath (BS), and mesophyll (M) cells. We have carried out a cell lineage analysis of these cell types using six spontaneous striping mutants of maize. We show that certain cell division patterns are preferentially utilized,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90304-7
更新日期：1989-05-01 00:00:00
abstract：:The nematode gonad is an exemplary system for the study of organogenesis and fundamental problems in developmental and cellular biology. Nematode gonads vary dramatically across species (Chitwood, B.G., Chitwood, M.B., 1950. Introduction to Nematology." University Park Press, Baltimore; Felix, M.A., Sternberg, P.W., 1...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.09.021
更新日期：2005-01-01 00:00:00
abstract：:The Id1 and Id3 genes play major roles during cardiac development, despite their expression being confined to non-myocardial layers (endocardium-endothelium-epicardium). We previously described that Id1Id3 double knockout (dKO) mouse embryos die at mid-gestation from multiple cardiac defects, but early lethality precl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.10.004
更新日期：2011-01-01 00:00:00
