Abstract:
:Cadherin-11 (cad-11) is a novel member of the cadherin family of cell adhesion molecules, having recently been identified by means of the polymerase chain reaction. To study the function and expression of this molecule, we cloned mouse, cad-11 cDNA. Transfection of L cells with cDNA led them to acquire a typical cadherin-dependent cell-cell adhesiveness, and the L cells expressing cad-11 did not coaggregate with L cells expressing E-, P-, N-, or R-cadherin when they were mixed, indicating that this novel cadherin has a homophilic binding specificity, as found for other cadherins. To determine the developmental expression pattern of this molecule, we performed in situ hybridization analysis on early mouse embryos. Cad-11 first appeared in mesodermal layers only in the head and tail regions at the mid-to-late primitive streak stages. In the head, this appearance was followed by strong expression in mesenchymal tissues including branchial arches. In the trunk, the paraxial mesoderm initially did not express cad-11. However, as the somites formed, they expressed cad-11, and this expression was strictly correlated with their initial condensation and segregation from the presomitic mesoderm. The cad-11 expression in the somites was eventually restricted to sclerotome cells. As the limb buds developed, cad-11 appeared in the distal portion of the limb mesenchyme, and, at later stages, its expression was most evident at the peripheral mesenchyme. Cad-11 was thus expressed by restricted populations of mesenchymal cells in early embryos, although it was also expressed in parts of the neural tube, such as the optic vesicle and dorsal midline, and in part of the otic vesicle. As a step to investigate the role of cad-11 in mesenchymal cell adhesion, we dissociated the limb bud mesenchyme into single cells, pelleted them, and cultured them as aggregates. In these cultures, cad-11-positive cells clearly sorted out of the negative cell population, suggesting that cad-11 might be involved in selective association of mesenchymal cells. For comparison, we studied the expression of N-cadherin and found that the expressions of these two cadherins were differential, and complementary in some tissues. These results suggest that cad-11 is involved in specific associations of subsets of mesenchymal cells and also of some neural cells during early embryogenesis.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Kimura Y,Matsunami H,Inoue T,Shimamura K,Uchida N,Ueno T,Miyazaki T,Takeichi Mdoi
10.1006/dbio.1995.1149subject
Has Abstractpub_date
1995-05-01 00:00:00pages
347-58issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(85)71149-9journal_volume
169pub_type
杂志文章abstract::Interleukin-6 is a member of a class of hormone-like molecules termed cytokines. The actions of IL-6 are highly pleiotropic. In adults IL-6 functions as a major mediator of inflammatory responses as well as inducing the synthesis of acute phase proteins by the liver following infection or injury. Based on in vitro and...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/0012-1606(92)90173-e
更新日期:1992-06-01 00:00:00
abstract::To investigate the spatiotemporal dynamics of skin pattern formation, we developed a simple method for artificially disarranging the placement of all three pigment cell types in the body trunk of zebrafish (Danio rerio). We generated transgenic fish with melanophores that ectopically expressed a variant of channelrhod...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.12.019
更新日期:2020-04-01 00:00:00
abstract::Extraocular muscles (EOM) represent a unique muscle group that controls eye movements and originates from head mesoderm, while the more typically studied body and limb muscles are somite-derived. Aiming to investigate myogenic progenitors (satellite cells) in EOM versus limb and diaphragm of adult mice, we have been u...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.08.010
更新日期:2014-01-15 00:00:00
abstract::The mechanism by which pluripotent progenitors give rise to distinct classes of mature neurons in vertebrates is not well understood. To address this issue we undertook a genetic screen for mutations which affect the commitment and differentiation of catecholaminergic (CA) [dopaminergic (DA), noradrenergic (NA), and a...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9204
更新日期:1999-04-15 00:00:00
abstract::Gr16 (chromosome 2, 6.9 cM) is a dominant, grey egg mutation of the domestic silkmoth, Bombyx mori, which produces an opaque instead of a translucent chorion. To determine the underlying cause of this defect, we examined the effects of the mutation on chorion ultrastructure, protein accumulation, and biosynthesis patt...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1112
更新日期:1993-05-01 00:00:00
abstract::The accumulation of total RNA transcripts of 18 late embryo-abundant (Lea) gene families, each encoding two closely related Lea mRNAs, was measured in cotyledon total RNA during embryogenesis and germination of Gossypium hirsutum L. by RNA dot hybridization. Transcript abundance of the three storage protein families w...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90442-8
更新日期:1987-09-01 00:00:00
abstract::The C. elegans Hox gene egl-5 (ortholog of Drosophila Abdominal-B) is expressed in multiple tissues in the tail region and is involved in tail patterning. In this study, we identify and clone the corresponding C. elegans orthologs of the components of the heterotrimeric transcription factor NFY, nfya-1, nfyb-1 and nfy...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.05.021
更新日期:2007-08-15 00:00:00
abstract::Pole cell formation in embryos of the parthenogenetic midge, Smittia sp., can be delayed or inhibited by irradiation of the posterior egg pole with ultraviolet light (uv). This leaves the schedule of nuclear divisions and chromosome eliminations virtually unaffected. However, uv irradiation delays the precocious migra...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90236-1
更新日期:1983-12-01 00:00:00
abstract::The distribution of the extracellular matrix protein tenascin was studied in the developing chick visual system to determine its possible regulatory role in retinotectal development. Little tenascin was present in the retinal optic fiber layer, and the optic nerve and tract, but was abundant in the stratum opticum of ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1076
更新日期:1993-03-01 00:00:00
abstract::A genetically complex mutant, two-faced (tfd), which causes the production of extra eye and antennal tissue on the dorsal head cuticle, has been analyzed for connectivity to and projection patterns in the central brain of nerves derived from these extra structures. It has been found that the extra antennal nerves freq...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90370-7
更新日期:1985-01-01 00:00:00
abstract::The smaller AB-cell of the 2-cell embryo of Tubifex divides 40 min later than the larger CD-cell. The present study was undertaken to elucidate those factors that are responsible for this cleavage asynchrony. An examination of chromosome cycles showed that the AB-cell entered M-phase 5 min later and spent about 35 min...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1123
更新日期:1993-05-01 00:00:00
abstract::Distal-less 3 (DLX3) gene mutations are etiologic for Tricho-Dento-Osseous syndrome. To investigate the in vivo impact of mutant DLX3 on bone development, we established transgenic (TG) mice expressing the c.571_574delGGGG DLX-3 gene mutation (MT-DLX3) driven by a mouse 2.3 Col1A1 promoter. Microcomputed tomographic a...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.10.014
更新日期:2009-01-01 00:00:00
abstract::The mature heart valves and septa are derived from the cardiac cushions which initially form as local outgrowths of mesenchymal cells within the outflow tract and atrioventricular regions. Endocardial cells respond to signals from the overlying myocardium and undergo an epithelial-to-mesenchymal transformation to inva...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0284
更新日期:2001-07-15 00:00:00
abstract::The HCO3(-) anion activates sperm motility, an important early step in capacitation, by increasing flagellar beat frequency through a pathway that requires the atypical adenylyl cyclase SACY and the sperm-specific C alpha2 catalytic subunit of PKA. Here we show that the accelerating action of HCO3(-) also requires the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.09.017
更新日期:2007-12-01 00:00:00
abstract::Bone Morphogenetic Proteins (BMPs) have multiple activities in the developing spinal cord: they specify the identity of the dorsal-most neuronal populations and then direct the trajectories of dorsal interneuron (dI) 1 commissural axons. How are these activities decoded by dorsal neurons to result in different cellula...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.05.014
更新日期:2012-07-15 00:00:00
abstract::In this study, we present evidence that neurogenic cells inhibit the differentiation of cardiogenic cells. When cells of the entire area pellucida at stage 5 were dissociated and reaggregated, the aggregates differentiated into neural tissues and other structures of any germ layer origin, except for heart tissues, des...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1266
更新日期:1995-09-01 00:00:00
abstract::Using an in vitro model of rat epiphyseal chondrocyte differentiation in which cells are maintained in a three-dimensional cell pellet, we show that exogenous TGF-beta 1 reversibly prevents terminal differentiation of epiphyseal chondrocytes into hypertrophic cells. Through maintenance of gene expression for the carti...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1200
更新日期:1993-08-01 00:00:00
abstract::Morphogenesis of the Drosophila tracheal system relies on different signalling pathways that have distinct roles in specifying both the migration of the tracheal cells and the particular morphological features of the primary branches. The current view is that the tracheal cells are initially specified as an equivalent...
journal_title:Developmental biology
pub_type: 杂志文章
doi:
更新日期:2002-10-15 00:00:00
abstract::Expression of the c-abl proto-oncogene in the mouse testis is characterized by the production of a unique 4.7-kb transcript present in germ cells that have entered the haploid phase of spermatogenesis. A similar developmental stage specificity of expression is observed for a member of the 70-kDa heat-shock protein (hs...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90222-9
更新日期:1988-02-01 00:00:00
abstract::RNA localization is tightly coordinated with RNA stability and translation control. Bicaudal-D (Bic-D), Egalitarian (Egl), microtubules and their motors are part of a Drosophila transport machinery that localizes mRNAs to specific cellular regions during oogenesis and embryogenesis. We identified the Poly(A)-binding p...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.07.009
更新日期:2011-09-15 00:00:00
abstract::Growth and Differentiation Factor 1 (GDF-1) has been implicated in left-right patterning of the mouse embryo but has no other known function. Here, we demonstrate a genetic interaction between Gdf1 and Nodal during anterior axis development. Gdf1-/-;Nodal+/- mutants displayed several abnormalities that were not presen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.02.002
更新日期:2006-05-15 00:00:00
abstract::The PITX1 transcription factor is expressed during hindlimb development, where it plays a critical role in directing hindlimb growth and the specification of hindlimb morphology. While it is known that PITX1 regulates hindlimb formation, in part, through activation of the Tbx4 gene, other transcriptional targets remai...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.12.013
更新日期:2018-02-01 00:00:00
abstract::The surface of eggs of the ascidian Halocynthia roretzi, observed with SEM, is essentially smooth until immediately before cell division when numerous microvilli appear and remain during cytokinesis. As the dividing blastomeres become closely adherent, however, the microvilli disappear and the eggs recover their smoot...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90214-8
更新日期:1984-04-01 00:00:00
abstract::The four members of the albumin gene family encode the serum transport proteins albumin, alpha-fetoprotein, alpha-albumin, and vitamin D-binding protein. These genes are transcribed primarily in the liver with each having a different pattern of developmental expression. The tight linkage of these genes, particularly t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.09.026
更新日期:2009-12-15 00:00:00
abstract::In warm-blooded vertebrates it is generally accepted that after early stages of development new neurons are not added to the retina. Contrary to this belief, we show here that hatched chickens have a zone of proliferating cells at the peripheral margin of the retina, similar to that of fish and amphibians. We found th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9640
更新日期:2000-04-15 00:00:00
abstract::Integrin α5-null embryos die in mid-gestation from severe defects in cardiovascular morphogenesis, which stem from defective development of the neural crest, heart and vasculature. To investigate the role of integrin α5β1 in cardiovascular development, we used the Mesp1(Cre) knock-in strain of mice to ablate integrin ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.09.014
更新日期:2014-11-15 00:00:00
abstract::Tie1 is a receptor tyrosine kinase expressed in endothelial cells, where it modulates Angiopoietin/Tie2 signaling. Previous studies have shown that mouse Tie1 mutants exhibit severe cardiovascular defects; however, much remains to be learned about the role of Tie1, especially during cardiac development. To further und...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2020.09.008
更新日期:2021-01-01 00:00:00
abstract::Chick sensory neurons grow to their correct targets in the hindlimb from the outset during normal development and following various experimental manipulations. This may result not because sensory neurons respond to specific limb-derived cues, but because they interact in some way with motoneurons which are responsive ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90023-0
更新日期:1986-12-01 00:00:00
abstract::Activin is a potent inducer of axial mesoderm in vitro and may have a similar role in vivo. Xenopus laevis eggs contain significant amounts of activin or activin-like factors, but maternal activin transcripts have not been detected in Xenopus eggs. The maternal activin protein might be translated from activin beta A o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1265
更新日期:1993-10-01 00:00:00
abstract::We have isolated a nonneuronal cell line from Xenopus retinal neuroepithelium (XR1 cell line). On the basis of immunocytochemical characterization using monoclonal antibodies generated in our laboratory as well as several other glial-specific antibodies, we have established that the XR1 cells are derived from embryoni...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90086-9
更新日期:1989-07-01 00:00:00