Abstract：
:CREB-2 (also called ATF4, TAXREB67, or C/ATF) is an evolutionarily conserved member of the CREB/ATF family of basic-leucine zipper transcription factors. CREB-2 is expressed ubiquitously in the adult mouse and can function as both a transcriptional activator and a repressor. However, little was understood about the normal function of CREB-2 in mammalian development or organ physiology. In this report we have used gene targeting to produce CREB-2-deficient (CREB-2-/-) mice. Adult CREB-2-/- mice displayed microphthalmia due to the complete absence of a lens. Early embryonic lens development including formation of the optic vesicle, primary lens fibers, and proliferating anterior epithelial cells occurred normally in these mice. However, beginning at ED 14.5 the CREB-2-deficient anterior epithelial lens cells underwent massive and synchronous apoptosis. This was followed by the complete resorption of the developing lens. Consistent with this defect in anterior epithelial cell survival, in situ hybridization studies showed that CREB-2 is expressed at high levels in wild-type anterior epithelial lens cells at ED 14.5. The defect in lens formation seen in the CREB-2-/- mice was not associated with qualitative defects in the expression of Pax-6, alphaA-crystallin, c-maf, or PDGF-R alpha. However, apoptosis of the anterior epithelial cells was mediated by a p53-dependent cell death pathway because ablation of the p53 gene rescued anterior epithelial cell death and allowed the formation of a lens in the absence of CREB-2. Taken together, these results identify CREB-2 as an important regulator of mammalian lens development.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Hettmann T,Barton K,Leiden JMdoi
10.1006/dbio.2000.9699subject
Has Abstractpub_date
2000-06-01 00:00:00pages
110-23issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(00)99699-4journal_volume
222pub_type
杂志文章abstract：:Primary cultures of cardiac myocytes from newborn normal and genetically cardiomyopathic (strain UM-X7.1) hamsters were analyzed by electron microscopy and immunofluorescent staining for myosin, actin, tropomyosin, and alpha-actinin. Antibody staining of these contractile proteins demonstrates that both normal and car...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90091-x
更新日期：1983-06-01 00:00:00
abstract：:Anuran amphibian embryos (Xenopus laevis and Rana dybowskii) are sensitive to novel gravitational fields. Under simulated weightlessness, (i) the location of the first horizontal cleavage furrow was shifted toward the vegetal pole at the eight-cell stage; (ii) the position of the blastocoel was more centered, and the ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1024
更新日期：1993-01-01 00:00:00
abstract：:The role of fibronectin in mesoderm cell migration and and the importance of mesoderm migration for gastrulation in Xenopus are examined. To allow for migration, a stable interface must exist between migrating mesoderm cells and the cells of the substrate layer, the blastocoel roof. We show that maintenance of this in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0174
更新日期：1996-08-01 00:00:00
abstract：:The neural cell adhesion molecule (NCAM) is thought to play a role in the formation of the vertebrate nervous system. In mammals and chicken, it is known that more than 100 different forms of the NCAM protein can be generated by alternative splicing of one primary transcript and it is possible that these different for...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90276-m
更新日期：1992-01-01 00:00:00
abstract：:Neural crest cells migrate along two pathways in the trunk: the ventral path, between the neural tube and somite, and the dorsolateral path, between the somite and overlying ectoderm. In avian embryos, ventral migration precedes dorsolateral migration by nearly 24 h, and the onset of dorsolateral migration coincides w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8963
更新日期：1998-08-15 00:00:00
abstract：:Unlike adult mammals, adult frogs regrow their optic nerve following a crush injury, making Xenopus laevis a compelling model for studying the molecular mechanisms that underlie neuronal regeneration. Using Translational Ribosome Affinity Purification (TRAP), a method to isolate ribosome-associated mRNAs from a target...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.06.003
更新日期：2017-06-15 00:00:00
abstract：:Sequence analysis of cDNA clones encoding fibronectin (FN) from Xenopus laevis reveals extensive amino acid identities with other vertebrate FNs, including the presence of the Arg-Gly-Asp (RGD) cell attachment site in type III-10 and of a second, cell-binding site (EILDV) in the alternative spliced V region of the pro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90291-n
更新日期：1992-02-01 00:00:00
abstract：:Msx1 and Msx2 are highly conserved, Nk-related homeodomain transcription factors that are essential for a variety of tissue-tissue interactions during vertebrate organogenesis. Here we show that combined deficiencies of Msx1 and Msx2 cause conotruncal anomalies associated with malalignment of the cardiac outflow tract...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.05.037
更新日期：2007-08-15 00:00:00
abstract：:Satellite cells, also known as muscle stem cells, are responsible for skeletal muscle growth and repair in mammals. Pax7 and Pax3 transcription factors are established satellite cell markers required for muscle development and regeneration, and there is great interest in identifying additional factors that regulate sa...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.03.004
更新日期：2017-04-15 00:00:00
abstract：:Prophase-arrested oocytes of Ruditapes philippinarum are triggered to undergo germinal vesicle breakdown under the influence of the neurohormone serotonin (5HT) and then arrest in metaphase 1. Our data show that these oocytes possess a single class of original 5HT receptors. Their binding parameters have been determin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1222
更新日期：1994-08-01 00:00:00
abstract：:Midline defects account for approximately 5% of congenital abnormalities observed at birth. However, the molecular mechanisms underlying the formation of the ventral body wall are not well understood. Recent studies linked mutations in Porcupine-an O-acetyl transferase mediating Wnt ligand acylation-with defects in th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.02.014
更新日期：2015-05-15 00:00:00
abstract：:BMPRIA is a receptor for bone morphogenetic proteins with high affinity for BMP2 and BMP4. Mouse embryos lacking Bmpr1a fail to gastrulate, complicating studies on the requirements for BMP signaling in germ layer development. Recent work shows that BMP4 produced in extraembryonic tissues initiates gastrulation. Here w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.048
更新日期：2004-06-01 00:00:00
abstract：:The axonal functions that act in the formation of the neuronal network have been shown to occur in close interdependence with the tissue that surrounds the growing axons. However, little is known about the molecular building blocks underlying axonal functions, although more than 400 axonal proteins have been identifie...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90090-4
更新日期：1986-11-01 00:00:00
abstract：:The development of supernumerary bristle precursors induced by the mutation shaggy (sgg; also known as zeste-white 3) was examined in the developing wing blade of imaginal and pupal Drosophila. sgg clones were induced by mitotic recombination; clones were marked using enhancer-trap flies which express beta-galactosida...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90134-3
更新日期：1992-08-01 00:00:00
abstract：:Chromatin insulators orchestrate gene transcription during embryo development and cell differentiation by stabilizing interactions between distant genomic sites. Mutations in genes encoding insulator proteins are generally lethal, making in vivo functional analyses of insulator proteins difficult. In Drosophila, howev...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.03.024
更新日期：2015-07-01 00:00:00
abstract：:The developing chick limb bud contains several stage-specific populations of muscle colony-forming (MCF) cells. The cell lineage relationships of these populations are not completely understood, but previous studies have suggested that there may be at least two myogenic precursor populations present in the limb bud. T...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90237-9
更新日期：1984-12-01 00:00:00
abstract：:The insect head is composed of several segments. During embryonic development, the segments fuse to form a rigid head capsule where obvious segmental boundaries are lacking. Hence, the assignment of regions of the insect head to specific segments is hampered, especially with respect to dorsal (vertex) and lateral (gen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.11.010
更新日期：2010-02-01 00:00:00
abstract：:Despite undergoing normal development and acquiring normal morphology and motility, mutations in spe-38 or trp-3/spe-41 cause identical phenotypes in Caenorhabditis elegans-mutant sperm fail to fertilize oocytes despite direct contact. SPE-38 is a novel, four-pass transmembrane protein and TRP-3/SPE-41 is a Ca(2+)-per...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.02.037
更新日期：2012-05-15 00:00:00
abstract：:The role of angiotensin II (Ang II) in the early embryonic development of the heart has not been examined. We have used RT-PCR to identify mRNA for angiotensinogen, angiotensin-converting enzyme, and the Ang II AT1 and AT2 receptors in embryonic day 10.25 Sprague-Dawley rats, and have used confocal microscopy to local...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8764
更新日期：1997-12-15 00:00:00
abstract：:Monoclonal antibodies against the surface of embryonic osteogenic cells (SB-1, SB-2, SB-3, and SB-5) have been used to characterize the sequence of transitions involved in the osteogenic cell lineage. In the present study, immunohistochemical analyses of the expression of osteogenic cell surface antigens in organ cult...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90388-y
更新日期：1990-10-01 00:00:00
abstract：:Epi 1, a monoclonal antibody, was generated against an epidermal specific epithelial antigen; it does not stain neural epithelium. We have used Epi 1 as a marker to determine when the spatial patterns delineating neural from nonneural epithelium become established. We used ventral ectoderm in a sandwich assay to show ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90307-2
更新日期：1989-05-01 00:00:00
abstract：:The nematode gonad is an exemplary system for the study of organogenesis and fundamental problems in developmental and cellular biology. Nematode gonads vary dramatically across species (Chitwood, B.G., Chitwood, M.B., 1950. Introduction to Nematology." University Park Press, Baltimore; Felix, M.A., Sternberg, P.W., 1...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.09.021
更新日期：2005-01-01 00:00:00
abstract：:We previously demonstrated that male mice deficient in the soluble adenylyl cyclase (sAC) are sterile and produce spermatozoa with deficits in progressive motility and are unable to fertilize zona-intact eggs. Here, analyses of sAC(-/-) spermatozoa provide additional insights into the functions linked to cAMP signalin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.05.038
更新日期：2006-08-15 00:00:00
abstract：:Cell proliferation, growth and survival are three different basic processes which converge at determining a fundamental property -the size of an organism. Scalloped (Sd) is the first characterised transcriptional partner to Yorkie (Yki), the downstream effector of the Hippo pathway which is a highly potential and evol...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.10.016
更新日期：2017-12-15 00:00:00
abstract：:In the course of embryogenesis multicellular structures and organs are assembled from constituent cells. One structural component common to many organs is the tube, which consists most simply of a luminal space surrounded by a single layer of epithelial cells. The notochord of ascidian Ciona forms a tube consisting of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.03.015
更新日期：2009-06-15 00:00:00
abstract：:Primary sensory olfactory neurons reside in a neuroepithelium lining the nasal cavity and project topographically onto the surface of the olfactory bulb, a rostral extension of the telencephalon. Galectin-1, a bivalent galactose-binding vertebrate lectin, is expressed in the developing rodent olfactory system. In the ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0257
更新日期：1996-10-10 00:00:00
abstract：:The uterus undergoes a series of dramatic changes in response to an implanting conceptus that, in some mammalian species, includes differentiation of the endometrial stroma into decidual tissue. This process, called decidualization, can be induced artificially in rodents indicating that the conceptus may not be essent...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.03.006
更新日期：2006-06-15 00:00:00
abstract：:Division of a female Drosophila stem cell produces a daughter stem cell and a cystoblast. The cystoblast produces a syncytial cluster of 16 cells by precisely four mitotic divisions and incomplete cytokinesis. Mutations in genes required for cystoblast differentiation, such as bag-of-marbles, block syncytial cluster f...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9346
更新日期：1999-08-15 00:00:00
abstract：:In higher organisms, mononucleated myoblasts fuse to form multinucleated myotubes. During this process, myoblasts undergo specific changes in cell morphology and cytoarchitecture. Previously, we have shown that the actin regulator Kette (Hem-2/Nap-1) is essential for myoblast fusion. In this study, we describe the rol...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.01.015
更新日期：2007-04-15 00:00:00
abstract：:Horseradish peroxidase (HRP) was used as an intracellular lineage tracer in two experiments designed to reveal the sites of origin of cells that formed the duplicate embryo which developed in relation to an organizer grafted in the ventral marginal zone (VMZ) of Xenopus laevis embryos. In the first experiment a dorsal...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90180-5
更新日期：1984-03-01 00:00:00
