Abstract：
:The role of fibronectin in mesoderm cell migration and and the importance of mesoderm migration for gastrulation in Xenopus are examined. To allow for migration, a stable interface must exist between migrating mesoderm cells and the cells of the substrate layer, the blastocoel roof. We show that maintenance of this interface does not depend on fibronectin. We further demonstrate that fibronectin contributes to, but is not essential for, mesoderm cell adhesion to the blastocoel roof. However, interaction with fibronectin is necessary for cell spreading and the formation of lamelliform cytoplasmic protrusions. Apparently, the specific role of fibronectin in mesoderm migration is to control cell protrusive activity. Consequently, when fibronectin function is blocked by GRGDSP peptide or antibodies, mesoderm cell migration is inhibited. Nevertheless, gastrulation proceeds nearly normally in inhibitor-treated embryos. It appears that in Xenopus, mesoderm migration is not essential for gastrulation.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Winklbauer R,Keller REdoi
10.1006/dbio.1996.0174subject
Has Abstractpub_date
1996-08-01 00:00:00pages
413-26issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(96)90174-8journal_volume
177pub_type
杂志文章abstract：:Chondrocytes were isolated from the auricular cartilage of rabbits, aged 1 week to 30 months, and grown in short-term cell culture. The cells from the 1-week animals were small, polygonal, and mononucleated, while the chondrocytes from the older animals were larger, rounded, and frequently binucleated. The synthesis o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90311-1
更新日期：1983-03-01 00:00:00
abstract：:Myogenic precursors in adult skeletal muscle (satellite cells) are mitotically quiescent but can proliferate in response to a variety of stresses including muscle injury. To gain further understanding of adult myoblasts, we analyzed myogenesis of satellite cells on intact fibers isolated from adult rat muscle. In this...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1226
更新日期：1994-08-01 00:00:00
abstract：:The heart is the first organ to form and function in the vertebrate embryo. Furthermore, differences between the left and right sides of the embryo become first detectable during cardiac development. We observed strong cardiac laterality phenotypes in medaka embryos by manipulating Groucho protein activity. The phenot...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.11.020
更新日期：2007-03-01 00:00:00
abstract：:During endochondral ossification, the differentiation of proliferating into hypertrophic chondrocytes is a key step determining the pace of bone formation and the future length of the skeletal elements. A variety of transcription factors are expressed at the onset of hypertrophy coordinating the expression of differen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.09.012
更新日期：2020-01-01 00:00:00
abstract：:Rex1 (Zfp42), GeneID 132625, is a gene whose expression is closely associated with pluripotency/multipotency in both mouse and human embryonic stem cells. To study the function of the murine Rex1 gene in vivo, we have used cre/lox technology to create Rex1(floxed) mice and mice deficient in Rex1 gene function. Rex1(-/...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.05.664
更新日期：2011-08-15 00:00:00
abstract：:We report the patterns of migration of Sertoli cells plated on specific substrata, and the influences of testicular peritubular cells on these processes. Data presented indicate that while peritubular cells readily spread when explanted onto Type I collagen, Sertoli cells do not. A delay of 4 to 6 days occurs after Se...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90114-4
更新日期：1986-01-01 00:00:00
abstract：:Cyclin J is a cyclin family member that appears to have evolved before the metazoan radiation. Its evolutionary conservation argues for an important role but functional characterizations of Cyclin J have remained very limited. In Drosophila, Cyclin J is expressed only in females. Using transgenic Drosophila lines expr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.06.042
更新日期：2009-09-15 00:00:00
abstract：:Immunological approaches were used to characterize an antigen that is present within the cortical granules of mouse oocytes and eggs. Immunoelectron microscopy shows a specific localization of the antigen to the cortical granules in the cortex of mouse oocytes and eggs. Following in vitro fertilization, the antigen is...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90392-v
更新日期：1990-10-01 00:00:00
abstract：:The Xenopus helix-loop-helix transcription factor Hairy2 is essential for neural crest progenitor survival and maintains cells in a mitotic undifferentiated pre-neural crest state. However, its mode of action remains largely unknown. Here we show that a Hairy2 DNA-binding mutant is unable to promote cell survival and ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.07.026
更新日期：2008-10-15 00:00:00
abstract：:Hes3 belongs to the Hes basic helix-loop-helix family of transcriptional repressors that play central roles in maintaining progenitor cells and regulating binary cell fate decisions in the embryo. During Xenopus laevis development, hes3 is expressed in the embryonic ectoderm in a horseshoe shape domain at the edge of ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.07.011
更新日期：2018-10-01 00:00:00
abstract：:The lizards are evolutionarily the closest vertebrates to humans that demonstrate the ability to regenerate entire appendages containing cartilage, muscle, skin, and nervous tissue. We previously isolated PAX7-positive cells from muscle of the green anole lizard, Anolis carolinensis, that can differentiate into multin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.08.037
更新日期：2018-01-15 00:00:00
abstract：:One function of the Wingless signaling pathway is to determine the naked, cuticle cell fate choice in the trunk epidermis of Drosophila larvae. The zinc finger protein Teashirt binds to the transactivator domain of Armadillo to modulate Wingless signaling output in the embryonic trunk and contributes to the naked cell...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0498
更新日期：2002-01-01 00:00:00
abstract：:Changes in the intracellular free calcium levels in the cortical region of cleaving Xenopus eggs were examined using semi-synthetic aequorin. We detected periodic oscillations in the free calcium levels accompanying the cleavage cycle as early as the first cleavage onward. The calcium levels began to increase at early...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1325
更新日期：1993-12-01 00:00:00
abstract：:The mouse Otlx2 gene is a new member of the paired-like family of homeobox genes whose human homologue, RIEG, is involved in Rieger syndrome, an autosomal-dominant disorder. One of the cardinal features of Rieger syndrome is dental hypoplasia, indicating that Otlx2/RIEG activity is essential for normal tooth developme...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8672
更新日期：1997-09-15 00:00:00
abstract：:Division of a female Drosophila stem cell produces a daughter stem cell and a cystoblast. The cystoblast produces a syncytial cluster of 16 cells by precisely four mitotic divisions and incomplete cytokinesis. Mutations in genes required for cystoblast differentiation, such as bag-of-marbles, block syncytial cluster f...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9346
更新日期：1999-08-15 00:00:00
abstract：:Caenorhabditis elegans embryonic elongation depends on both epidermal and muscle cells. The hemidesmosome-like junctions, commonly called fibrous organelles (FOs), that attach the epidermis to the extracellular matrix ensure muscle anchoring to the cuticular exoskeleton and play an essential role during elongation. To...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.11.025
更新日期：2011-02-15 00:00:00
abstract：:The facial primordia in the chick embryo begin as rounded swellings that surround the primitive mouth and these grow out to form the beak. The control of proximodistal outgrowth is not well understood but may involve similar mechanisms to the limb bud. In order to test this hypothesis, combinations were made between e...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90069-s
更新日期：1992-12-01 00:00:00
abstract：:The nematode gonad is an exemplary system for the study of organogenesis and fundamental problems in developmental and cellular biology. Nematode gonads vary dramatically across species (Chitwood, B.G., Chitwood, M.B., 1950. Introduction to Nematology." University Park Press, Baltimore; Felix, M.A., Sternberg, P.W., 1...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.09.021
更新日期：2005-01-01 00:00:00
abstract：:Tabby is a mouse mutant characterized by deficient development of the ectodermal organs: teeth, hair, and a subset of glands. Ectodysplasin, the protein encoded by the Tabby gene, was recently identified as a novel TNF-like transmembrane protein but little is known about its function. We have examined the Tabby tooth ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9514
更新日期：1999-12-15 00:00:00
abstract：:Global silencing of transcriptional activity in the oocyte genome occurs just before the resumption of meiosis and is a crucial developmental transition at the culmination of oogenesis. Transcriptionally quiescent oocytes rely on stored maternal transcripts to sustain the completion of meiosis, fertilization, and earl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.08.028
更新日期：2004-11-15 00:00:00
abstract：:To investigate the molecular regulation of embryonic somite formation and development, we have cloned the full-length cDNA and characterized the embryonic expression profile of chicken Paraxis, a member of a novel family of basic helix-loop-helix (bHLH) proteins, which has been suggested to play a role in paraxial mes...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8663
更新日期：1997-09-01 00:00:00
abstract：:Migration of Anterior Visceral Endoderm (AVE) is a critical symmetry breaking event in the early post-implantation embryo development and is essential for establishing the correct body plan. Despite much effort, cellular and molecular events influencing AVE migration are only partially understood. Here, using time-lap...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.03.016
更新日期：2019-06-15 00:00:00
abstract：:The N-ethyl-N-nitrosourea (ENU) ←forward genetic screen is a useful tool for the unbiased discovery of novel mechanisms regulating developmental processes. We recovered the dorothy mutation in such a screen designed to recover recessive mutations affecting craniofacial development in the mouse. Dorothy embryos die pre...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.10.039
更新日期：2020-02-15 00:00:00
abstract：:Cell signaling pathways, such as Wnt, Hedgehog (Hh), Notch, and Hippo, are essential for embryogenesis, organogenesis, and tissue homeostasis. In this study, we analyzed 415 genes involved in these pathways in the allotetraploid frog, Xenopus laevis. Most genes are retained in two subgenomes called L and S (193 homeol...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.12.006
更新日期：2017-06-15 00:00:00
abstract：:Auxin is implicated throughout plant growth and development. Although the effects of this plant hormone have been recognized for more than a century, it is only in the past two decades that light has been shed on the molecular mechanisms that regulate auxin homeostasis, signaling, transport, crosstalk with other hormo...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2012.05.039
更新日期：2012-09-01 00:00:00
abstract：:During primary neurogenesis in Xenopus, a cascade of helix--loop--helix (HLH) transcription factors regulates neuronal determination and differentiation. While XNeuroD functions at a late step in this cascade to regulate neuronal differentiation, the factors that carry out terminal differentiation are still unknown. W...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0230
更新日期：2001-05-15 00:00:00
abstract：:The contribution of cranial neural crest cells to the development and patterning of cranial muscles in amphibians was investigated in the phylogenetically basal and morphologically generalized frog, Bombina orientalis. Experimental methods included fluorescent marking of premigratory cranial neural crest and extirpati...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0377
更新日期：2001-09-15 00:00:00
abstract：:Eggs have developed their own strategies for early development. Amphibian, teleost fish, and ascidian eggs show cortical rotation and an accompanying structure, a cortical parallel microtubule (MT) array, during the one-cell embryonic stage. Cortical rotation is thought to relocate maternal deposits to a certain compa...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2018.11.019
更新日期：2019-04-15 00:00:00
abstract：:Sine Oculis (So), the founding member of the SIX family of homeobox transcription factors, binds to sequence specific DNA elements and regulates transcription of downstream target genes. It does so, in part, through the formation of distinct biochemical complexes with Eyes Absent (Eya) and Groucho (Gro). While these c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.03.012
更新日期：2014-06-15 00:00:00
abstract：:Rapidly growing human teratocarcinoma cells (Tera-2) can be induced to differentiate into quiescent, nontumorigenic cells expressing neuronal markers. To more closely mimic the in vivo conditions for tumor growth, we grew Tera-2 cells in three-dimensional collagen gel cultures. The undifferentiated cells proliferated ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1016
更新日期：1994-01-01 00:00:00
