Abstract：
:The neural cell adhesion molecule (NCAM) is thought to play a role in the formation of the vertebrate nervous system. In mammals and chicken, it is known that more than 100 different forms of the NCAM protein can be generated by alternative splicing of one primary transcript and it is possible that these different forms have distinct biological functions. A large part of the diversity is generated by alternative mRNA splicing in two regions, called the pi and the muscle specific domain (MSD), that encode portions of the extracellular domain of the NCAM protein. In this report, we describe the tissue and developmental expression of the pi and MSD sequences in the amphibian, Xenopus laevis. Our experiments show that NCAM transcripts are present in all tissues examined including muscle, heart, liver, kidney, and brain. We have identified a 30-base exon, similar to the pi domain observed in mammals, that is not present in maternal NCAM RNA but appears in a subset of the NCAM mRNA population shortly after neural induction. At the predicted location of the MSD we have detected only two alternatively spliced exons, 3 bases and 15 bases in length. In no X. laevis tissue examined did we detect the two additional alternatively spliced exons which are present in the MSD region of mammalian and chicken NCAM RNAs. Finally, the analysis has revealed a dynamic and complex pattern of expression of alternatively spliced NCAM mRNAs during embryogenesis. High levels of expression of specific forms of NCAM RNA correlate with major morphogenic events such as neural tube formation and metamorphosis.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Zorn AM,Krieg PAdoi
10.1016/0012-1606(92)90276-msubject
Has Abstractpub_date
1992-01-01 00:00:00pages
197-205issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(92)90276-Mjournal_volume
149pub_type
杂志文章abstract：:Cell-cell fusion is a fundamental cellular process that is essential for development as well as fertilization. Myoblast fusion to form multinucleated skeletal muscle myotubes is a well studied, yet incompletely understood example of cell-cell fusion that is essential for formation of contractile skeletal muscle tissue...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.10.035
更新日期：2009-01-15 00:00:00
abstract：:Two distinct DNA ligases are expressed during early sea urchin embryogenesis. A light form (50 kDa) is found in unfertilized eggs (oocyte form) and a heavier enzyme (110 kDa) is observed at the two-cell stage (embryonic form). The chronology of the change reveals that the embryonic form is detected 90 min after fertil...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90479-9
更新日期：1987-11-01 00:00:00
abstract：:Here we describe a novel set of peptidergic neurons conserved throughout all developmental stages in the Drosophila central nervous system (CNS). We show that a small complement of 28 apterous-expressing cells (Ap-let neurons) in the ventral nerve cord (VNC) of Drosophila larvae co-express numerous gene products. The ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.015
更新日期：2004-05-01 00:00:00
abstract：:In developing limb buds, Msx2 transcripts are expressed in the apical ectodermal ridge (AER) and in various regions of the limb mesenchyme. To identify DNA sequences responsible for Msx2 expression in the AER, we characterized the expression of LacZ reporter constructs driven by chicken Msx2 regulatory sequences in tr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.03.005
更新日期：2004-06-15 00:00:00
abstract：:The release of intracellular calcium (Ca2+) via either inositol 1,4, 5-trisphosphate receptors (IP3R) or ryanodine receptors (RyR) activates a wide variety of signaling pathways in virtually every type of cell. In the present study we demonstrate that at early stages of development IP3R mRNA and functional IP3-gated C...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9120
更新日期：1999-02-15 00:00:00
abstract：:Zygotes and embryos of the fucoid brown alga Pelvetia compressa undergo a series of asymmetric cleavages. We are interested in the developmental role of these cleavages and the mechanism controlling their alignment. To assess the importance of division plane alignment, the orientation of the first asymmetric division ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8832
更新日期：1998-02-15 00:00:00
abstract：:The homebox gene Hlxb9, encoding Hb9, exhibits a dual expression profile during pancreatic development. The early expression in the dorsal and ventral pancreatic epithelium is transient and spans from embryonic day (e) 8 to e9-e10, whereas the later expression is confined to differentiating beta-cells as they appear. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0440
更新日期：2001-12-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90339-2
更新日期：1985-05-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9911
更新日期：2000-11-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.01.001
更新日期：2018-02-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90439-3
更新日期：1988-11-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90243-9
更新日期：1983-12-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.07.023
更新日期：2007-10-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0310
更新日期：1996-11-25 00:00:00
abstract：:Recent studies with amoebae of the Myxomycetes Didymium iridis and Physarum polycephalum reveal that competence to undergo sexual fusion or to differentiate to plasmodia apogamically can be induced by a diffusible factor. Suspension cultures of Didymium amoebae have proven to be a good source of the inducer activity. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90337-3
更新日期：1984-06-01 00:00:00
abstract：:We present a genetic, developmental and molecular analysis of lethal(1)discs large-1[l(1)d.lg-1; Stewart et al., 1972], an oncogene of Drosophila. Mutations in this gene cause the imaginal discs to grow by cell proliferation beyond their normal final size, transform into solid tumors, fuse with one another and the bra...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90092-4
更新日期：1989-07-01 00:00:00
abstract：:Mammalian eggs remain arrested at metaphase of the second meiotic division (metII) for an indeterminate time before fertilization. During this period, which can last several hours, the continued attachment of sister chromatids is thought to be achieved by inhibition of the protease separase. Separase is known to be in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.06.036
更新日期：2008-09-15 00:00:00
abstract：:A key step in organogenesis of the Drosophila tracheal system is the integration of isolated tracheal metameres into a connected tubular network. The interaction of tracheal cells with surrounding mesodermal cells is crucial in this process. In particular, single mesodermal cells called bridge-cells are essential for ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.04.462
更新日期：2006-08-01 00:00:00
abstract：:We have investigated oocyte development in Hydra vulgaris, a member of one of the oldest metazoan phyla. We show that oocyte determination involves a mechanism that establishes a subset of precursor interstitial cells competent to differentiate into oocytes. The oocyte is singled out from this subset and the competenc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9790
更新日期：2000-08-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0199
更新日期：2001-05-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.03.018
更新日期：2007-06-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.03.021
更新日期：2008-06-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.07.032
更新日期：2006-11-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1068
更新日期：1994-03-01 00:00:00
abstract：:To gain an understanding of the genes and mechanisms that govern morphogenesis and its evolution, we have analyzed mutations that disrupt this process in a simple model structure, the male tail tip of the rhabditid nematode C. elegans. During the evolution of rhabditid male tails, there have been several independent c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.04.472
更新日期：2006-09-01 00:00:00
abstract：:The outstanding migration and differentiation capacities of neural crest cells (NCCs) have fascinated scientists since Wilhelm His described this cell population in 1868. Today, after intense research using vertebrate model organisms, we have gained considerable knowledge regarding the origin, migration and differenti...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.05.012
更新日期：2010-08-15 00:00:00
abstract：:Metaphase II arrest of Xenopus oocyte is characterized by the presence of M-phase-promoting factor (MPF) and of a microtubular spindle, both of which are stable in the presence of protein synthesis inhibitors. We studied in vivo this equilibrium state that is settled during meiotic maturation. At time of germinal vesi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8543
更新日期：1997-05-01 00:00:00
abstract：:In the early Drosophila embryo, Bone morphogenetic protein (BMP) activity is positively and negatively regulated by the BMP-binding proteins Short gastrulation (Sog) and Twisted gastrulation (Tsg). We show here that a similar mechanism operates during crossvein formation, utilizing Sog and a new member of the tsg gene...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.02.029
更新日期：2005-06-01 00:00:00
abstract：:We have investigated the sequential changes of acetylcholine receptor (AChR) distribution on identified Xenopus laevis muscle cells in culture before and after innervation. AChRs on muscle cells were stained with tetramethylrhodamine-conjugated alpha-bungarotoxin and the distribution of AChR clusters was examined on a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90006-x
更新日期：1984-05-01 00:00:00
abstract：:Retinoic acid (RA) signaling is required for normal development of multiple organs. However, little is known about how RA influences the initial stages of lung development. Here, we used a combination of genetic, pharmacological and explant culture approaches to address this issue, and to investigate how signaling by ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.10.045
更新日期：2006-03-01 00:00:00
