Abstract:
:Macronuclei of Tetrahymena thermophila contain a typical H1 which has been shown to be missing from micronuclei. Instead, micronuclei contain three unique polypeptides, alpha, beta, and gamma, which are associated with linker regions of micronuclear chromatin. In this report polyclonal antibodies raised against macronuclear H1 are shown to react with alpha, beta, and gamma by immunoblotting analyses. This result suggests that these polypeptides share some common structural feature(s). Also consistent with this result is the finding that both macro- and micronuclei in growing and mating cells stain positively with H1 antibodies by in situ indirect immunofluorescence. However, these analyses demonstrate that the level of linker histone is greatly reduced in the micronucleus of starved cells and in young macronuclear anlagen. These results are in agreement with earlier biochemical studies and together provide strong evidence that dramatic changes in linker histone accompany nuclear differentiation (and dedifferentiation) in Tetrahymena.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Chicoine LG,Wenkert D,Richman R,Wiggins JC,Allis CDdoi
10.1016/0012-1606(85)90339-2subject
Has Abstractpub_date
1985-05-01 00:00:00pages
1-8issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(85)90339-2journal_volume
109pub_type
杂志文章abstract::The organizer is an essential signaling center required for axial formation during vertebrate embryonic development. In the basal chordate amphioxus, the dorsal blastopore lip of the gastrula has been proposed to be homologous to the vertebrate organizer. Lefty is one of the first genes to be expressed in the organize...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.08.006
更新日期:2019-12-01 00:00:00
abstract::Aurora A is a mitotic kinase essential for cell proliferation. In mice, ablation of Aurora A results in mitotic arrest and pre-implantation lethality, preventing studies at later stages of development. Here we report the effects of Aurora A ablation on embryo patterning at early post-implantation stages. Inactivation ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.08.010
更新日期:2012-11-01 00:00:00
abstract::We recently demonstrated the requirement of two-pore channel type 2 (TPC2)-mediated Ca2+ release during slow muscle cell differentiation and motor circuit maturation in intact zebrafish embryos. However, the upstream trigger(s) of TPC2/Ca2+ signaling during these developmental processes remains unclear. Nicotinic acid...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.11.005
更新日期:2019-01-15 00:00:00
abstract::Vertebrate voluntary muscles are composed of myotubes and connective tissue cells. These two cell types have different embryonic origins: myogenic cells arise from paraxial mesoderm, while in the head many of the connective tissues are formed by neural crest cells. The objective of this research was to study interacti...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90138-7
更新日期:1986-08-01 00:00:00
abstract::This investigation provides the first systematic determination of the cellular and molecular progression of vocal fold (VF) epithelium development in a murine model. We define five principal developmental events that constitute the progression from VF initiation in the embryonic anterior foregut tube to fully differen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.12.037
更新日期:2015-03-15 00:00:00
abstract::Calcium influx is known to be prerequisite for membrane fusion of myoblasts. However, little is known about the channels that are responsible for the entry of calcium into the cells. Here we show that K(Ca) channels and stretch-activated channels are involved in the calcium influx. Upon analysis of single-channel reco...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0091
更新日期:1996-04-10 00:00:00
abstract::The formation and maintenance of the highly regular organization of membrane systems and proteins in striated muscle require specific membrane-membrane and membrane-cytoskeleton interactions. The development of T-tubules and sarcoplasmic reticulum (SR) was followed in gastrocnemius muscle fibers from chicken embryos b...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1292
更新日期:1993-11-01 00:00:00
abstract::Complex intrinsic and extrinsic mechanisms determine neural cell fate during development of the nervous system. Using Id4 deficient mice, we show that Id4 is required for normal development of the central nervous system (CNS), timing neural differentiation in the developing forebrain. In the absence of Id4, the ventri...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.02.001
更新日期:2005-04-15 00:00:00
abstract::A mouse mammary epithelial cell line with morphogenetic properties in vivo, Comma-Dbeta, was used to isolate and to characterize mammary progenitor cells. We found that a homogeneous cell population expressing high surface levels of stem cell antigen 1 (Sca-1) was able to give rise in vivo to ductal and alveolar struc...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.02.007
更新日期:2006-05-15 00:00:00
abstract::pmar1 is a transcription factor in the paired class homeodomain family that was identified and found to be transcribed in micromeres beginning at the fourth cleavage of sea urchin development [Dev. Biol. 246 (2002), 209]. Based on in situ data, molecular perturbation studies, and QPCR data, the recently published gene...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00108-8
更新日期:2003-06-01 00:00:00
abstract::The subdivision of the developing field by region-specific expression of genes encoding transcription factors is an essential step during appendage development in arthropod and vertebrates. In Drosophila leg development, the distal-most region (pretarsus) is specified by the expression of homeobox genes, aristaless an...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.12.005
更新日期:2005-03-15 00:00:00
abstract::Prophase-arrested oocytes of Ruditapes philippinarum are triggered to undergo germinal vesicle breakdown under the influence of the neurohormone serotonin (5HT) and then arrest in metaphase 1. Our data show that these oocytes possess a single class of original 5HT receptors. Their binding parameters have been determin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1222
更新日期:1994-08-01 00:00:00
abstract::Suppression of wing fate and specification of haltere fate in Drosophila by the homeotic gene Ultrabithorax is a classical example of Hox regulation of serial homology (Lewis, E.B. 1978. Nature 276, 565-570) and has served as a paradigm for understanding homeotic gene function. We have used DNA microarray analyses to ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.12.022
更新日期:2006-03-15 00:00:00
abstract::The development of the reproductive system in Caenorhabditis elegans is a well-established model system for patterning and organogenesis. We report the characterization of the cog-1 gene, mutations in which cause novel phenotypes in late patterning in vulval lineages, establishment of the vulva-uterine connection, dev...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0850
更新日期:2002-12-15 00:00:00
abstract::We show that the intermediate filament protein nestin is expressed in myogenic cells and that multiple mechanisms regulate nestin expression at different stages of myogenesis. Cultured embryonic, fetal, and neonatal mouse limb myoblasts initially expressed nestin in the absence of the four muscle regulatory factors (M...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1248
更新日期:1994-09-01 00:00:00
abstract::The role of fibronectin in mesoderm cell migration and and the importance of mesoderm migration for gastrulation in Xenopus are examined. To allow for migration, a stable interface must exist between migrating mesoderm cells and the cells of the substrate layer, the blastocoel roof. We show that maintenance of this in...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0174
更新日期:1996-08-01 00:00:00
abstract::Brain 4 (Brn4/Pou3f4) and Pax6 are POU-homeodomain and paired-homeodomain transcription factors, respectively, that are expressed in the brain and the glucagon-expressing cells in the pancreas. Brn4 expression begins at embryonic day 10 in the pancreas, just before pax6 and both appear in the glucagon immunoreactive c...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.12.008
更新日期:2004-04-01 00:00:00
abstract::NK genes are related pan-metazoan homeobox genes. In the fruitfly, NK genes are clustered and involved in patterning various mesodermal derivatives during embryogenesis. It was therefore suggested that the NK cluster emerged in evolution as an ancestral mesodermal patterning cluster. To test this hypothesis, we cloned...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.02.013
更新日期:2008-05-15 00:00:00
abstract::Planarians are well-known for their exceptional regenerative abilities. This investigation focuses on the involvement of a Y-box protein, defined by the presence of a cold-shock domain, in regeneration-specific processes. Previous studies have shown that developmentally expressed Y-box proteins bind to mRNA molecules ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8996
更新日期:1998-09-15 00:00:00
abstract::The oblique muscle layer in the leech body wall is built upon the processes of a unique identified embryonic cell, the Comb- or C-cell. Each C-cell is composed of a spindle-shaped soma that projects approximately 70 parallel processes through the developing body wall at an angle oblique to the long axis. The morphogen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90342-z
更新日期:1991-11-01 00:00:00
abstract::Previous studies from this laboratory (M. Bronner-Fraser (1985). J. Cell Biol. 101, 610) have demonstrated that an antibody to a cell surface receptor complex caused alterations in avian neural crest cell migration. Here, these observations are extended to examine the distribution and persistency of injected antibody,...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90320-9
更新日期:1986-10-01 00:00:00
abstract::Eight series of mouse chimeras were produced by aggregating 8-cell embryos that differed at the Gpi-1s locus, encoding glucose phosphate isomerase (GPI-1). Chimeric blastocysts (Gpi-1sa/Gpi-1sa <--> Gpi-1sb/Gpi-1sb) were transferred to pseudopregnant females, which produced only GPI-1C enzyme. Quantitative electrophor...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1062
更新日期:1995-03-01 00:00:00
abstract::Hair shafts are produced from stem cells located in the bulge. Our knowledge of the genetic pathways regulating cell fate acquisition in the immediate descendents of these stem cells, and fate maintenance in their committed progeny, is still incomplete. One pathway involved in fate maintenance within the hair matrix i...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.11.021
更新日期:2009-02-15 00:00:00
abstract::Hedgehog (Hh) proteins are secreted molecules that play an essential role in development and tumorigenesis. In Drosophila cultured cells, phosphorylation of the kinesin-like Costal2 (Cos2) protein at Ser572 is triggered by the kinase fused (Fu) upon Hh pathway activation. Here, we validate the first phospho-antibody f...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.04.021
更新日期:2010-08-01 00:00:00
abstract::Leaf primordia, first visible as small bumps, are produced in a cyclical pattern at the edges of the shoot apex, a smooth region at the top of the stem. Their formation is a biomechanical process. This review first considers hypothetical construction mechanisms and then summarizes research that provides information ab...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/0012-1606(92)90089-y
更新日期:1992-09-01 00:00:00
abstract::The mammalian kidney collecting ducts are critical for water, electrolyte and acid-base homeostasis and develop as a branched network of tubular structures composed of principal cells intermingled with intercalated cells. The intermingled nature of the different collecting duct cell types has made it challenging to id...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.02.007
更新日期:2017-04-01 00:00:00
abstract::To determine whether low oxygen is a stimulus for endothelial cell differentiation and vascular development in the kidney, we examined the effect of low oxygen on rat metanephric organ culture, a model known to recapitulate nephrogenesis in the absence of vessels. After 6 days in culture in standard (20% O2) or low ox...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8513
更新日期:1997-03-15 00:00:00
abstract::Septins are guanosine triphosphate-binding proteins that are evolutionally conserved in all eukaryotes other than plants. They function as multimeric complexes that interact with membrane lipids, actomyosin, and microtubules. Based on these interactions, septins play essential roles in the morphogenesis and physiologi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.09.012
更新日期:2016-11-15 00:00:00
abstract::The dorsal air sacs supply oxygen to the flight muscles of the Drosophila adult. This tracheal organ grows from an epithelial tube (the air sac primordium (ASP)) that arises during the third larval instar (L3) from a wing-disc-associated tracheal branch. Since the ASP is generated by a program of both morphogenesis an...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.09.005
更新日期:2005-11-01 00:00:00
abstract::The distribution of the extracellular matrix protein tenascin was studied in the developing chick visual system to determine its possible regulatory role in retinotectal development. Little tenascin was present in the retinal optic fiber layer, and the optic nerve and tract, but was abundant in the stratum opticum of ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1076
更新日期:1993-03-01 00:00:00