Abstract：
:We show that the intermediate filament protein nestin is expressed in myogenic cells and that multiple mechanisms regulate nestin expression at different stages of myogenesis. Cultured embryonic, fetal, and neonatal mouse limb myoblasts initially expressed nestin in the absence of the four muscle regulatory factors (MRFs) of the MyoD family, whereas nestin and MRFs became coexpressed by myoblasts as culture duration was lengthened. Upon differentiation, nestin was commonly concentrated at the ends, and reduced or absent in the middles, of myotubes formed by mouse limb cells. Nestin was expressed by C2C12 and L6 myoblasts and was distributed throughout C2C12 myotubes, but was entirely absent in myotubes formed by L6 cells, suggesting that nestin is dispensable for fusion and terminal differentiation. Nestin was expressed in C3H10T1/2 cells, but was not expressed in 3T3-L1 cells until transfected with MyoD or myogenin. In mouse somites, nestin was found in both myotomal and dermatomal cells. Thus, nestin is expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis, and nestin expression can be activated upon MRF transfection. Additional MRF-independent mechanisms must, however, regulate nestin expression, because nestin is found in MRF-negative cells and, conversely, nestin is not uniformly distributed in MRF-expressing myotubes.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Kachinsky AM,Dominov JA,Miller JBdoi
10.1006/dbio.1994.1248subject
Has Abstractpub_date
1994-09-01 00:00:00pages
216-28issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(84)71248-6journal_volume
165pub_type
杂志文章abstract：:In embryos of Patella vulgata at the 32-cell stage, one of the four vegetally located macromeres makes contacts with overlying animal micromeres. As a result, this macromere (designated 3D) divides significantly later than the other macromeres and forms the mesodermal stem cell 4d. Shortly before and during this inter...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90239-x
更新日期：1989-04-01 00:00:00
abstract：:Ovarian germ cell survival is dependent upon the formation of primordial follicles, which occurs during fetal life in the human. Activin contributes to germ cell proliferation and survival at this time. SMADs2 and 3 are central elements in the activin signalling pathway and thus indicate sites of activin action. We ha...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.11.026
更新日期：2008-02-01 00:00:00
abstract：:Using optical imaging assays, we investigated the dynamics of acetylcholine receptors (AChRs) at laminin-associated clusters on cultured myotubes in the absence or presence of the nerve-derived clustering factor, agrin. Using fluorescence recovery after photobleaching (FRAP) on fluorescent bungarotoxin-labeled recepto...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.09.041
更新日期：2005-12-01 00:00:00
abstract：:The thisbe (ths) gene encodes a Drosophila fibroblast growth factor (FGF), and mutant females are viable but sterile suggesting a link between FGF signaling and fertility. Ovaries exhibit abnormal morphology including lack of epithelial sheaths and muscle tissues that surround ovarioles. Here we investigated how FGF i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.04.023
更新日期：2015-08-01 00:00:00
abstract：:Connexin 43 (Cx43) knockout mice and transgenic mice (CMV43) overexpressing the Cx43 gap junction gene exhibit heart defects involving the conotruncus and right ventricle. Based on the heart phenotype and Cx43 gene and transgene expression pattern, we previously proposed that the heart defects may reflect a role for g...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8891
更新日期：1998-06-01 00:00:00
abstract：:We report the patterns of migration of Sertoli cells plated on specific substrata, and the influences of testicular peritubular cells on these processes. Data presented indicate that while peritubular cells readily spread when explanted onto Type I collagen, Sertoli cells do not. A delay of 4 to 6 days occurs after Se...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90114-4
更新日期：1986-01-01 00:00:00
abstract：:Early mammalian embryogenesis is controlled by mechanisms governing the balance between pluripotency and differentiation. The expression of early lineage-specific genes can vary significantly between species, with implications for developmental control and stem cell derivation. However, the mechanisms involved in patt...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.12.008
更新日期：2013-03-01 00:00:00
abstract：:The regulation of Sertoli cell transferrin and sulfated glycoprotein-2 (SGP-2) mRNA levels by vitamin A was studied in vitamin A deficient rats. Vitamin A deficiency differentially affected the levels of these mRNAs. Sertoli cells from vitamin A deficient rats contained 3-fold less transferrin mRNA and 1.8-fold more S...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90167-9
更新日期：1987-06-01 00:00:00
abstract：:We investigated whether the inhibition of proteoglycan synthesis and salivary branching morphogenesis by beta-D-xyloside was related to the deposition and processing of newly synthesized glycosaminoglycans at the basal epithelial surface that correlates with normal branching activity. Forty eight-hour cultures of cont...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90358-6
更新日期：1985-05-01 00:00:00
abstract：:Primordial germ cells (PGCs) are the embryonic precursors of the adult gametes. Although restricted in developmental potency, PGCs express many of the same molecular markers as pluripotent embryonic stem (ES) cells and can give rise to embryonal carcinoma (EC) cells, the stem cells of testicular tumors, in vivo. Likew...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.08.029
更新日期：2007-11-15 00:00:00
abstract：:The segregation of founder cells from the somatic mesoderm is a prerequisite for the formation of body wall muscles in the Drosophila embryo. The myogenic basic helix-loop-helix protein, Nautilus (Nau), is expressed in a subset of these founder cells in medial and lateral positions in the somatic mesoderm. Mutations i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.9987
更新日期：1996-05-25 00:00:00
abstract：:Acute myeloid leukemia (AML) is a complex malignancy with poor prognosis. Several genetic lesions can lead to the disease. One of these corresponds to the NUP98-HOXA9 (NA9) translocation that fuses sequences encoding the N-terminal part of NUP98 to those encoding the DNA-binding domain of HOXA9. Despite several studie...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.11.003
更新日期：2017-01-01 00:00:00
abstract：:Epigenetic gene control is involved in mechanisms of development. Little is known about the cooperation of nuclear and chromatin events in programmed differentiation from mouse embryonic stem cells (ESC). To address this, Oct3/4-positive ESC and differentiated progenies, Sox1-positive neural precursor cells (NPC) and ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.04.450
更新日期：2006-10-15 00:00:00
abstract：:In the embryonic mouse retina, retinoic acid (RA) is unevenly distributed along the dorsoventral axis: RA-rich zones in dorsal and ventral retina are separated by a horizontal RA-poor stripe that contains the RA-inactivating enzyme CYP26A1. To explore the developmental role of this arrangement, we studied formation of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.08.032
更新日期：2004-12-01 00:00:00
abstract：:There is a natural curiosity about how organisms give rise to offspring like themselves through a series of reproducible developmental events and how, once mature, these offspring mate and continue the process giving rise the next generation. In the mid-1800s investigators started using gametes and embryos to explore ...
journal_title：Developmental biology
pub_type： 历史文章,杂志文章
doi：10.1016/j.ydbio.2011.06.021
更新日期：2011-10-15 00:00:00
abstract：:Pancreatic development depends on the transcription factor Pax6, which controls islet cell differentiation and hormone production. To understand the regulation of Pax6 pancreatic expression, we have identified a minimal Pax6 pancreatic enhancer and show that it contains a composite binding site for Meis and Pbx homeop...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.06.030
更新日期：2006-12-15 00:00:00
abstract：FOREWORD:The neural crest has been the main object of my investigations during my career in science, up to now. It is a fascinating topic for an embryologist because of its two unique characteristics: its large degree of multipotency and the fact that its development involves a phase during which its component cells mi...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2018.07.019
更新日期：2018-12-01 00:00:00
abstract：:The early mouse embryo undertakes two types of cell division: symmetric that gives rise to the trophectoderm and then placenta or asymmetric that gives rise to inner cells that generate the embryo proper. Although cell division orientation is important, the mechanism regulating it has remained unclear. Here, we identi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.05.009
更新日期：2014-08-15 00:00:00
abstract：:Effective transgenesis methods have been successfully employed in many organisms including zebrafish. However, accurate spatiotemporal control of transgene expression is still difficult to achieve. Here we describe a system for chemical-inducible gene expression and demonstrate its feasibility for generating transgeni...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.042
更新日期：2008-08-01 00:00:00
abstract：:Cloning and sequencing of Tripneustes gratilla genomic DNA and cDNA encoding a developmentally regulated, embryonic messenger RNA, referred to as Tg616, revealed an actin-encoding gene orthologous to the CyI actin gene described from Strongylocentrotus purpuratus. Tg616 and SpCyI share: (1) 150 nucleotides of highly c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1239
更新日期：1994-09-01 00:00:00
abstract：:In vertebrates, the segmented somites, which are the medial-most component in the paraxial mesoderm, are the entity giving rise to the axial bones and skeletal muscles. We previously demonstrated that the mechanism that distinguishes the somite from the more lateral mesoderm (lateral plate) involves different levels o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8895
更新日期：1998-10-15 00:00:00
abstract：:Cell proliferation, growth and survival are three different basic processes which converge at determining a fundamental property -the size of an organism. Scalloped (Sd) is the first characterised transcriptional partner to Yorkie (Yki), the downstream effector of the Hippo pathway which is a highly potential and evol...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.10.016
更新日期：2017-12-15 00:00:00
abstract：:Transcription factors are key orchestrators of the emergence of neuronal diversity within the developing spinal cord. As such, the two paralogous proteins Pax3 and Pax7 regulate the specification of progenitor cells within the intermediate neural tube, by defining a neat segregation between those fated to form motor c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.06.014
更新日期：2017-12-01 00:00:00
abstract：:In mammals, two spatially and temporally distinct waves of fiber cell differentiation are crucial steps for normal lens development. In between these phases, an anterior growth zone forms in which progenitor cells migrate circumferentially, terminally exit the cell cycle and initiate differentiation at the lens equato...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.01.015
更新日期：2009-04-01 00:00:00
abstract：:The organizer is an essential signaling center required for axial formation during vertebrate embryonic development. In the basal chordate amphioxus, the dorsal blastopore lip of the gastrula has been proposed to be homologous to the vertebrate organizer. Lefty is one of the first genes to be expressed in the organize...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.08.006
更新日期：2019-12-01 00:00:00
abstract：:Tyrosinase is the rate-limiting enzyme for the production of melanin pigmentation. In the mouse and other animals, homozygous null mutations in the Tyrosinase gene (Tyr) result in the absence of pigmentation, i.e. albinism. Here we used the CRISPR/Cas9 system to generate mono- and bi-allelic null mutations in the Tyr ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.06.017
更新日期：2014-09-01 00:00:00
abstract：:We have previously shown that the Caenorhabditis elegans gut-specific esterase gene (Ce-ges-1) has the unusual ability to be expressed in different modules of the embryonic digestive tract (anterior pharynx, posterior pharynx, and rectum) depending on sequence elements within the Ce-ges-1 promoter. In the present pape...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0442
更新日期：2001-11-15 00:00:00
abstract：:Urodeles begin gastrulation with much of their presumptive mesoderm in the superficial cell layer, all of which must move into the deep layers during development. We studied the morphogenesis of superficial mesoderm in the urodeles Ambystoma maculatum, Ambystoma mexicanum, and Taricha granulosa. In all three species, ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0718
更新日期：2002-08-15 00:00:00
abstract：:The mammalian prostate arises from the urogenital sinus and few factors have been identified to be important in the early stages of prostate development. In this study we show that the transcription factor Sox9 is expressed in the epithelia of all mouse prostatic lobes from the initial stages of their development. We ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.01.030
更新日期：2008-04-15 00:00:00
abstract：:We recently demonstrated the requirement of two-pore channel type 2 (TPC2)-mediated Ca2+ release during slow muscle cell differentiation and motor circuit maturation in intact zebrafish embryos. However, the upstream trigger(s) of TPC2/Ca2+ signaling during these developmental processes remains unclear. Nicotinic acid...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.11.005
更新日期：2019-01-15 00:00:00
