Abstract：
:Acute myeloid leukemia (AML) is a complex malignancy with poor prognosis. Several genetic lesions can lead to the disease. One of these corresponds to the NUP98-HOXA9 (NA9) translocation that fuses sequences encoding the N-terminal part of NUP98 to those encoding the DNA-binding domain of HOXA9. Despite several studies, the mechanism underlying NA9 ability to induce leukemia is still unclear. To bridge this gap, we sought to functionally dissect NA9 activity using Drosophila. For this, we generated transgenic NA9 fly lines and expressed the oncoprotein during larval hematopoiesis. This markedly enhanced cell proliferation and tissue growth, but did not alter cell fate specification. Moreover, reminiscent to NA9 activity in mammals, strong cooperation was observed between NA9 and the MEIS homolog HTH. Genetic characterization of NA9-induced phenotypes suggested interference with PVR (Flt1-4 RTK homolog) signaling, which is similar to functional interactions observed in mammals between Flt3 and HOXA9 in leukemia. Finally, NA9 expression was also found to induce non-cell autonomous effects, raising the possibility that its leukemia-inducing activity also relies on this property. Together, our work suggests that NA9 ability to induce blood cell expansion is evolutionarily conserved. The amenability of NA9 activity to a genetically-tractable system should facilitate unraveling its molecular underpinnings.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Baril C,Gavory G,Bidla G,Knævelsrud H,Sauvageau G,Therrien Mdoi
10.1016/j.ydbio.2016.11.003subject
Has Abstractpub_date
2017-01-01 00:00:00pages
16-26issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(16)30503-6journal_volume
421pub_type
杂志文章abstract：:The mouse protamine mRNAs, Prm-1 and Prm-2, are translationally repressed for several days during male germ cell differentiation. The translational delay of mouse Prm-1 mRNA has previously been shown to be dependent upon cis-acting elements that reside in the last 62 nucleotides of the Prm-1 3' untranslated region (3'...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8705
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abstract：:HB-GAM (heparin-binding growth-associated molecule) is a secretory, extracellular matrix-associated protein that was isolated by screening for proteins that enhance neurite outgrowth in perinatal rat brain neurons. In the present study we have investigated the possible role of HB-GAM in cell proliferation in the devel...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0199
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pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
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abstract：:The acquisition of fertilizability in coelomic eggs of Xenopus laevis has been shown to be correlated with the physical, biochemical, and ultrastructural alterations of the egg envelope [coelomic envelope (CE)] induced during the passage of eggs through the pars recta portion of the oviduct. However, no direct evidenc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9285
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.07.035
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1236
更新日期：1993-09-01 00:00:00
abstract：:Malpighian tubules are the osmoregulatory and detoxifying organs of Drosophila and its proper development is critical for the survival of the organism. They are made up of two major cell types, the ectodermal principal cells and mesodermal stellate cells. The principal and stellate cells are structurally and physiolog...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.11.003
更新日期：2015-02-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.019
更新日期：2008-07-15 00:00:00
abstract：:An anterior-posterior concentration difference of fibronectin associated with the endoderm in early chick embryos has been implicated in the directional migration of precardiac mesoderm cells. We have examined the effect of increasing concentrations of an antibody to fibronectin (FN) to test the essentiality of FN to ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90378-8
更新日期：1988-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1101
更新日期：1993-04-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.06.035
更新日期：2017-09-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9618
更新日期：2000-03-15 00:00:00
abstract：:Molecular mechanisms regulating the remodeling of the lymphatic vasculature from an immature plexus of vessels to a hierarchal network of initial and collecting lymphatics are not well understood. One gene thought to be important for this process is Angiopoietin-2 (Ang-2). Ang2(-/-) mice have previously been reported ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.024
更新日期：2008-07-15 00:00:00
abstract：:Retinoic acid (RA) is a potent inducer of cell differentiation and plays an essential role in sex-specific germ cell development in the mammalian gonad. RA is essential for male gametogenesis and hence fertility. However, RA can also disrupt sexual cell fate in somatic cells of the testis, promoting transdifferentiati...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.02.015
更新日期：2017-04-15 00:00:00
abstract：:To gain an understanding of the genes and mechanisms that govern morphogenesis and its evolution, we have analyzed mutations that disrupt this process in a simple model structure, the male tail tip of the rhabditid nematode C. elegans. During the evolution of rhabditid male tails, there have been several independent c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.04.472
更新日期：2006-09-01 00:00:00
abstract：:Fibroblast growth factor (FGF) signalling has been implicated in the generation of mesoderm and neural fates in chordate embryos including ascidians and vertebrates. In Ciona, FGF9/16/20 has been implicated in both of these processes. However, in FGF9/16/20 knockdown embryos, notochord fate recovers during later devel...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.08.075
更新日期：2007-02-01 00:00:00
abstract：:The distribution and ontogeny of four neuropeptides in developing chick lumbosacral sensory and sympathetic ganglia were studied using immunohistochemical techniques. Antibodies to two of these peptides, substance P (SP) and calcitonin gene-related peptide (CGRP), stained small neurons in the medial part of the dorsal...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90137-5
更新日期：1986-08-01 00:00:00
abstract：:Planarians are well-known for their exceptional regenerative abilities. This investigation focuses on the involvement of a Y-box protein, defined by the presence of a cold-shock domain, in regeneration-specific processes. Previous studies have shown that developmentally expressed Y-box proteins bind to mRNA molecules ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8996
更新日期：1998-09-15 00:00:00
abstract：:Although mitogen-activated protein kinase (MAPK) is a well-known cell cycle regulator, emerging studies have also implicated its activity in the regulation of intracellular calcium concentration ([Ca2+](i)) and secretion. Those studies raise the hypothesis that MAPK activity during oocyte maturation and early fertiliz...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.03.029
更新日期：2007-06-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0653
更新日期：2002-06-15 00:00:00
abstract：:The members of the transforming growth factor-beta (TGF-beta) superfamily are secreted proteins that interact with cell-surface receptors to elicit signals that regulate a variety of biological processes during vertebrate embryogenesis. Alk2, also known as ActRIA, Tsk7L, and SKR1, encodes a type I TGF-beta family rece...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9378
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abstract：:Germ cells provide maternal mRNAs that are stored in the oocyte, and later translated at a specific time of development. In this context, gene regulation depends mainly on post-transcriptional mechanisms that contribute to keep maternal transcripts in a stable and translationally silent state. In recent years, small n...
journal_title：Developmental biology
pub_type： 杂志文章,评审
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更新日期：2016-08-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1994-02-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2015-09-01 00:00:00
abstract：:The Toll signaling pathway functions in several Drosophila processes, including dorsal-ventral pattern formation and the immune response. Here, we demonstrate that this pathway is required in the epidermis for proper muscle development. Previously, we showed that the zygotic Toll protein is necessary for normal muscle...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1998-07-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章,评审
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更新日期：2019-07-01 00:00:00
abstract：:Chondrocytes were isolated from the auricular cartilage of rabbits, aged 1 week to 30 months, and grown in short-term cell culture. The cells from the 1-week animals were small, polygonal, and mononucleated, while the chondrocytes from the older animals were larger, rounded, and frequently binucleated. The synthesis o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90311-1
更新日期：1983-03-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.12.015
更新日期：2006-05-15 00:00:00
