Abstract:
:Planarians are well-known for their exceptional regenerative abilities. This investigation focuses on the involvement of a Y-box protein, defined by the presence of a cold-shock domain, in regeneration-specific processes. Previous studies have shown that developmentally expressed Y-box proteins bind to mRNA molecules and regulate the timing of their translation. We have isolated and characterized a planarian Y-box gene, DjY1, which is specifically expressed at the site of regeneration, the blastema. DjY1 transcripts appear rapidly at the site of cutting and increase in number as the blastema grows. The timing and level of expression is similar irrespective of the orientation of the cut: in anterior, posterior, and lateral regenerative tissue. As regeneration nears completion, there is a general decrease in transcript level except in structures which are still differentiating, specifically in the auricles where new DjY1 transcripts are produced. A similarly modulated temporal pattern of expression throughout regeneration is seen in assaying the DjY1 protein. Within the population of blastemal cells, a subset of differentiating cells is specifically immunostained using antibodies to DjY1. The DjY1 protein contains a cold-shock domain and RG-repeat motifs, both of which are associated with RNA-binding properties: in vitro binding studies using recombinant DjY1 show that the preferred template is single-stranded RNA of heterogeneous sequence. These data provide the first direct evidence that a Y-box protein is involved in the regeneration process in planarians and implicate DjY1 in the translational regulation of differentiation-specific mRNAs.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Salvetti A,Batistoni R,Deri P,Rossi L,Sommerville Jdoi
10.1006/dbio.1998.8996subject
Has Abstractpub_date
1998-09-15 00:00:00pages
217-29issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(98)98996-5journal_volume
201pub_type
杂志文章abstract::Vertebrate voluntary muscles are composed of myotubes and connective tissue cells. These two cell types have different embryonic origins: myogenic cells arise from paraxial mesoderm, while in the head many of the connective tissues are formed by neural crest cells. The objective of this research was to study interacti...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90138-7
更新日期:1986-08-01 00:00:00
abstract::The genetic and developmental bases for trait expression and variation in adults are largely unknown. One system in which genes and cell behaviors underlying adult traits can be elucidated is the larval-to-adult transformation of zebrafish, Danio rerio. Metamorphosis in this and many other teleost fishes resembles amp...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00015-0
更新日期:2003-04-15 00:00:00
abstract::Sex in the nematode Caenorhabditis elegans is normally determined by the X chromosome to autosome (X:A) ratio, with XX hermaphrodites and XO males. Previous work has shown that a set of at least four autosomal genes (her-1, tra-2, tra-3, and tra-1) is signaled by the X:A ratio and appears to act in a regulatory pathwa...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90077-0
更新日期:1984-11-01 00:00:00
abstract::Thyroid hormone (TH) induces dramatic skeletal and tissue remodeling of the anuran head and body at metamorphosis. The expression pattern of TH up-regulated genes has been correlated with tissues that either grow or resorb at metamorphosis. Whereas the expression of the thyroid hormone receptors in Xenopus laevis tadp...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8975
更新日期:1998-11-01 00:00:00
abstract::The Toll signaling pathway functions in several Drosophila processes, including dorsal-ventral pattern formation and the immune response. Here, we demonstrate that this pathway is required in the epidermis for proper muscle development. Previously, we showed that the zygotic Toll protein is necessary for normal muscle...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8915
更新日期:1998-07-01 00:00:00
abstract::The gene nhr-6 encodes the Caenorhabditis elegans ortholog of the NR4A nuclear receptor. We determined the biological functions of NHR-6 through the isolation and characterization of a deletion allele of nhr-6, lg6001. We demonstrate that nhr-6 has an essential role in the development of the C. elegans somatic gonad. ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.11.014
更新日期:2008-01-15 00:00:00
abstract::Cranial placodes are specialized ectodermal regions in the developing vertebrate head that give rise to both neural and non-neural cell types of the neuroendocrine system and the sense organs of the visual, olfactory and acoustic systems. The cranial placodes develop from a panplacodal region which is specifically mar...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.10.024
更新日期:2011-02-01 00:00:00
abstract::Tyrosinase is the rate-limiting enzyme for the production of melanin pigmentation. In the mouse and other animals, homozygous null mutations in the Tyrosinase gene (Tyr) result in the absence of pigmentation, i.e. albinism. Here we used the CRISPR/Cas9 system to generate mono- and bi-allelic null mutations in the Tyr ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.06.017
更新日期:2014-09-01 00:00:00
abstract::The GRAM domain was found in glucosyltransferases, myotubularins and other membrane-associated proteins. So far, functions for majority of these proteins are yet to be uncovered. In order to address the evolutionary and functional significance of this family members, we have performed a comprehensive investigation on ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.11.031
更新日期:2008-02-15 00:00:00
abstract::Fertilization results in the tyrosine phosphorylation of several egg proteins within minutes of sperm-egg binding and inhibitor studies have shown that tyrosine kinase activity is required for many aspects of egg activation. The present study demonstrates the presence of p59c-fyn kinase in the sea urchin egg and exami...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0073
更新日期:1996-03-15 00:00:00
abstract::The Ci-Dll-B gene is an early regulator of ectodermal development in the ascidian Ciona intestinalis (Imai et al., 2006). Ci-Dll-B is located in a convergently transcribed bigene cluster with a tandem duplicate, Ci-Dll-A. This clustered genomic arrangement is the same as those of the homologous vertebrate Dlx genes, w...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.02.009
更新日期:2011-05-15 00:00:00
abstract::nautilus (nau), one member of the myogenic regulatory family of bHLH-encoding genes, is expressed in a subset of muscle precursors and differentiated fibers in the Drosophila embryo. To elucidate the role of nautilus in myogenesis, we have misexpressed it using the GAL4-targeted system. We find that ectopic expression...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.8434
更新日期:1997-01-15 00:00:00
abstract::During development of Dictyostelium discoideum, the cellular specific activity of beta-glucosidase increases before aggregation, declines to low levels during pseudoplasmodium formation, and increases rapidly during culmination. In addition, two electrophoretically distinct isozymes of beta-glucosidase are present at ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90407-6
更新日期:1987-10-01 00:00:00
abstract::The adrenal cortex has a complex vasculature that is essential for growth, tissue maintenance, and access of secreted steroids to the bloodstream. However, the interaction between vasculature and adrenal cortex during early organogenesis remains largely unclear. In this study, we focused on the zebrafish counterpart o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.04.464
更新日期:2006-09-01 00:00:00
abstract::Fertilization-related sperm mitochondrial movement occurs at a rate comparable to other actin-myosin-driven movements and is inhibited by cytochalasin B and N-ethyl maleimide in Ascidia ceratodes sperm. F-actin was demonstrated in the tails and mitochondria using NBD-phallacidin fluorescence. Both actin and myosin wer...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90229-x
更新日期:1984-12-01 00:00:00
abstract::The hallmark of the arthropods is their segmented body, although origin of segmentation, however, is unresolved. In order to shed light on the origin of segmentation we investigated orthologs of pair rule genes (PRGs) and segment polarity genes (SPGs) in a member of the closest related sister-group to the arthropods, ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.07.010
更新日期:2013-10-01 00:00:00
abstract::The four members of the albumin gene family encode the serum transport proteins albumin, alpha-fetoprotein, alpha-albumin, and vitamin D-binding protein. These genes are transcribed primarily in the liver with each having a different pattern of developmental expression. The tight linkage of these genes, particularly t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.09.026
更新日期:2009-12-15 00:00:00
abstract::A cell surface protein (3B11) is differentially expressed in the embryonic labial segment of Manduca as two circular monolayers of epithelial cells invaginate and segregate from surrounding epithelial cells. The cells that invaginate and preferentially express 3B11 represent the presumptive prothoracic glands. These c...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.8437
更新日期:1997-01-01 00:00:00
abstract::Connective tissue outgrowths of neonatal muscle onto a substratum of bone matrix differentiate into cartilage in response to a bone morphogenetic protein (BMP). The BMP can be separated from bone matrix by extraction with 4 M guanidine hydrochloride (GuHCl) or degraded in situ by endogenous proteolytic enzymes to deac...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90339-9
更新日期:1988-12-01 00:00:00
abstract::The epithelial-mesenchymal transition of cardiac endothelium is a critical developmental event in the formation of valvular and septal anlagen. We have demonstrated previously that this event can be mimicked in culture by treating atrioventricular canal (AV) endothelium with EDTA-soluble proteins extracted from embryo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90121-i
更新日期:1991-06-01 00:00:00
abstract::To investigate the mechanism by which the hormone 17 alpha,20 beta-dihydroxy-4-pregnen-3-one(17 alpha,20 beta-DP) acts on a receptor on the external surface of rainbow trout oocytes to induce maturation, the interaction between 17 alpha,20 beta-DP receptors and G-proteins was examined. Pertussis toxin (PT) catalyzed t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1341
更新日期:1994-12-01 00:00:00
abstract::Summary Sentence: Conditional ablation of AP-2 gamma results in a delay in skin development and abnormal expression of p63, K14, K1, filaggrin, repetin and secreted Ly6/Plaur domain containing 1, key genes required for epidermal development and differentiation. The development of the epidermis, a stratified squamous e...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.02.017
更新日期:2008-05-01 00:00:00
abstract::Lens growth involves the proliferation of epithelial cells, followed by their migration to the equator region and differentiation into secondary fiber cells. It is widely accepted that fibroblast growth factor (FGF) signaling is required for the differentiation of lens epithelial cells into crystallin-rich fibers, but...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.05.029
更新日期:2012-08-01 00:00:00
abstract::Sex-determination in Caenorhabditis elegans requires regulation of gene transcription and protein activity and stability. sel-10 encodes a WD40-repeat-containing F-box protein that likely mediates the ubiquitin-mediated degradation of important sex-determination factors. Loss of sel-10 results in a mild masculinizatio...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.07.035
更新日期:2008-10-15 00:00:00
abstract::The vertebrate liver and heart arise from adjacent cell layers in the anterior lateral (AL) endoderm and mesoderm of late gastrula embryos, and the earliest stages of liver and heart development are interrelated through reciprocal tissue interactions. Although classical embryological studies performed several decades ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.01.019
更新日期:2004-04-15 00:00:00
abstract::A fusion gene construct containing the bacterial chloramphenicol acetyltransferase (CAT) gene under the control of CyIIIa actin gene regulatory sequences was injected into unfertilized eggs of the urchin Strongylocentrotus purpuratus, and early pluteus stage embryos that developed from these eggs were fixed and sectio...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90193-x
更新日期:1987-06-01 00:00:00
abstract::Pluripotent stem cells, termed embryonic germ (EG) cells, have been generated from both human and mouse primordial germ cells (PGCs). Like embryonic stem (ES) cells, EG cells have the potential to differentiate into all germ layer derivatives and may also be important for any future clinical applications. The developm...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.11.007
更新日期:2008-01-15 00:00:00
abstract::Oligodendrocytes are produced from the same region of the ventral spinal cord that earlier generated motor neurons in bird and rodent embryos. Motor neuron and oligodendrocyte precursor cells express Olig genes, which encode basic helix-loop-helix transcription factors that play important roles in the development of b...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0738
更新日期:2002-08-15 00:00:00
abstract::The development of the Drosophila leg is a good model to study processes of pattern formation, cell death and segmentation. Such processes require the coordinate activity of different genes and signaling pathways that progressively subdivide the leg territory into smaller domains. One of the main pathways needed for l...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.10.011
更新日期:2014-01-15 00:00:00
abstract::Trophoblast cells are characterized by an invasive behavior into the surrounding uterine tissue. In rodents, an early peri-/endovascular type of invasion exerted by trophoblast giant cells can be distinguished from a late interstitial type carried out by glycogen trophoblast cells. Analysis of the molecular mechanisms...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.03.040
更新日期:2004-07-15 00:00:00