Abstract：
:Trophoblast cells are characterized by an invasive behavior into the surrounding uterine tissue. In rodents, an early peri-/endovascular type of invasion exerted by trophoblast giant cells can be distinguished from a late interstitial type carried out by glycogen trophoblast cells. Analysis of the molecular mechanisms of trophoblast invasion has been hampered, however, by the complex temporal and spatial patterns of invasion. We utilized trophoblast stem (TS) cell lines to study trophoblast invasion in vitro and to establish a model that facilitates investigation of this process on the molecular level. Our results showed that trophoblast giant cells that differentiate from TS cells in vitro are capable of penetrating a reconstituted basement membrane matrix. Consequently, invasion rates were increased in various giant cell differentiation-promoting conditions. We also derived TS cell lines that are homozygous for a mutation of the Hand1 transcription factor. The Hand1-/- TS cells showed reduced levels of giant cell differentiation and exhibited an approximately 50% decrease in invasion rates. In summary, trophoblast giant cells that differentiate from TS cells in vitro recapitulate the invasive capacity of normal trophoblast cells in vivo. The TS cell system is a valuable tool to identify and quantitatively study regulators of trophoblast invasion.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Hemberger M,Hughes M,Cross JCdoi
10.1016/j.ydbio.2004.03.040subject
Has Abstractpub_date
2004-07-15 00:00:00pages
362-71issue
2eissn
0012-1606issn
1095-564Xpii
S0012160604002738journal_volume
271pub_type
杂志文章abstract：:The formation of primary mesenchyme cells in euechinoid sea urchin embryos involves the transformation of 32 epithelial cells into mesenchymal cells in a process referred to as ingression. The mechanism that drives this epithelial-mesenchymal transformation has yet to be identified. Previous studies (J. R. Gibbins, L....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(89)80058-2
更新日期：1989-01-01 00:00:00
abstract：:Sequence analysis of cDNA clones encoding fibronectin (FN) from Xenopus laevis reveals extensive amino acid identities with other vertebrate FNs, including the presence of the Arg-Gly-Asp (RGD) cell attachment site in type III-10 and of a second, cell-binding site (EILDV) in the alternative spliced V region of the pro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90291-n
更新日期：1992-02-01 00:00:00
abstract：:MHM is a chicken Z chromosome-linked locus that is methylated and transcriptionally silent in male cells, but is hypomethylated and transcribed into a long non-coding RNA in female cells. MHM has been implicated in both localised dosage compensation and sex determination in the chicken embryo, but direct evidence is l...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.03.025
更新日期：2012-06-15 00:00:00
abstract：:Clotting is critical in limiting hemolymph loss and initiating wound healing in insects as in vertebrates. It is also an important immune defense, quickly forming a secondary barrier to infection, immobilizing bacteria and thereby promoting their killing. However, hemolymph clotting is one of the least understood immu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.03.019
更新日期：2006-07-01 00:00:00
abstract：:The elongation of embryo and tissue is a key morphogenetic event in embryogenesis and organogenesis. Notochord, a typical chordate organ, undergoes elongation to perform its regulatory roles and to form the structural support in the embryo. Notochord elongation is morphologically similar across all chordates, but asci...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2018.11.009
更新日期：2019-04-15 00:00:00
abstract：:The cDNA encoding the TATA binding protein was isolated from 8- to 16-cell and morula-stage embryonic libraries of two distantly related species of sea urchin, Strongylocentrotus purpuratus and Lytechinus variegatus, respectively. The two proteins are 96% identical over both the N- and C-terminal domains, suggesting a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9052
更新日期：1998-12-01 00:00:00
abstract：:nautilus (nau), one member of the myogenic regulatory family of bHLH-encoding genes, is expressed in a subset of muscle precursors and differentiated fibers in the Drosophila embryo. To elucidate the role of nautilus in myogenesis, we have misexpressed it using the GAL4-targeted system. We find that ectopic expression...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.8434
更新日期：1997-01-15 00:00:00
abstract：:The dorsal air sacs supply oxygen to the flight muscles of the Drosophila adult. This tracheal organ grows from an epithelial tube (the air sac primordium (ASP)) that arises during the third larval instar (L3) from a wing-disc-associated tracheal branch. Since the ASP is generated by a program of both morphogenesis an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.09.005
更新日期：2005-11-01 00:00:00
abstract：:Sex in the nematode Caenorhabditis elegans is normally determined by the X chromosome to autosome (X:A) ratio, with XX hermaphrodites and XO males. Previous work has shown that a set of at least four autosomal genes (her-1, tra-2, tra-3, and tra-1) is signaled by the X:A ratio and appears to act in a regulatory pathwa...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90077-0
更新日期：1984-11-01 00:00:00
abstract：:The vertebrate cranial base is a complex structure composed of bone, cartilage and other connective tissues underlying the brain; it is intimately connected with development of the face and cranial vault. Despite its central importance in craniofacial development, morphogenesis and tissue origins of the cranial base h...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.07.016
更新日期：2008-10-01 00:00:00
abstract：:Somatic embryogenesis is one of the best examples of the remarkable developmental plasticity of plants, in which committed somatic cells can dedifferentiate and acquire the ability to form an embryo and regenerate an entire plant. In Arabidopsis thaliana, the shoot apices of young seedlings have been reported as an al...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.04.023
更新日期：2018-10-01 00:00:00
abstract：:The development of the vertebrate inner ear is a complex process that has been investigated in several model organisms. In this work, we examined genetic interactions regulating early development of otic structures in medaka. We demonstrate that misexpression of Fgf8, Dlx3b and Foxi1 during early gastrulation is suffi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.04.022
更新日期：2007-07-15 00:00:00
abstract：:The murine cpk mouse develops a rapid-onset polycystic kidney disease (PKD) with many similarities to human PKD. During kidney development, the transcription factor Pax2 is required for the specification and differentiation of the renal epithelium. In humans, Pax2 is also expressed in juvenile cystic kidneys where it ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9618
更新日期：2000-03-15 00:00:00
abstract：:Skeletal muscles of the avian limb are derived from mononucleated myogenic precursor cells (myoblasts) that migrate into the somatopleural mesoderm of the developing limb bud from the ventrolateral dermomyotome of limb adjacent somites. In the present study, we utilized replication-deficient lacZ-encoding retroviruses...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00028-3
更新日期：2003-01-15 00:00:00
abstract：:The Drosophila melanogaster ventral nerve cord derives from neural progenitor cells called neuroblasts. Individual neuroblasts have unique gene expression profiles and give rise to distinct clones of neurons and glia. The specification of neuroblast identity provides a cell intrinsic mechanism which ultimately results...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.01.016
更新日期：2006-04-15 00:00:00
abstract：:BMP signaling responses are refined by distinct secreted and intracellular antagonists in different cellular and temporal contexts. Here, we show that the nuclear LEM-domain protein MAN1 is a tissue-specific antagonist of BMP signaling in Drosophila. MAN1 contains two potential Mad-binding sites. We generated MAN1Delt...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.11.036
更新日期：2010-03-01 00:00:00
abstract：:The effect of phenylephrine, an alpha-1 adrenergic agonist, on development of body asymmetry was studied using a rat whole embryo culture system. Embryos were explanted at the presomite stage, cultured in 100% rat serum containing various concentrations of phenylephrine, and examined at the 20-25 somite stage for side...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90067-d
更新日期：1991-01-01 00:00:00
abstract：:Although a large superfamily of G-protein-coupled receptors serves multiple functions, little is known about their functional activation during ontogeny. To examine the functional activation of the mu-opioid receptor (MOR) and the delta-opioid receptor (DOR) during development, sections of mouse embryos and fetuses fr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0855
更新日期：2003-01-01 00:00:00
abstract：:For both the intricate morphogenetic layout of the sensory cells in the ear and the elegantly radial arrangement of the sensory neurons in the nose, numerous signaling molecules and genetic determinants are required in concert to generate these specialized neuronal populations that help connect us to our environment. ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2014.01.023
更新日期：2014-05-01 00:00:00
abstract：:The cortex and subcortical regions of the developing follicles and eggs of silkmoths are rich in cytoskeletal elements, particularly actin. In situ analysis using [3H]-polyuridylic acid and biotinylated oligo d(T) reveals a pattern of changes in poly(A)+ RNA distribution during oogenesis. The developing pattern of dis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1031
更新日期：1993-02-01 00:00:00
abstract：:Auxin is implicated throughout plant growth and development. Although the effects of this plant hormone have been recognized for more than a century, it is only in the past two decades that light has been shed on the molecular mechanisms that regulate auxin homeostasis, signaling, transport, crosstalk with other hormo...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2012.05.039
更新日期：2012-09-01 00:00:00
abstract：:In many animals, the first cue for development is transcripts and/or proteins that are provided maternally and are localized at specific regions of fertilized eggs and early embryos. The ascidian is known to exhibit a mosaic mode of development, which is largely dependent on localized maternal factors. In early Ciona ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.05.018
更新日期：2006-08-15 00:00:00
abstract：:The expression of the neural cell adhesion molecules N-CAM and L1 was investigated in the olfactory system of the mouse using immunocytochemical and immunochemical techniques. In the olfactory epithelium, globose basal cells and olfactory neurons were stained by the polyclonal N-CAM antibody reacting with all three co...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90397-1
更新日期：1988-10-01 00:00:00
abstract：:The vertebrate intestinal epithelium is renewed continuously from stem cells at the base of the crypt in mammals or base of the fold in fish over the life of the organism. As stem cells divide, newly formed epithelial cells make an initial choice between a secretory or enterocyte fate. This choice has previously been ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.01.013
更新日期：2013-04-15 00:00:00
abstract：:The extracellular matrix of Xenopus laevis oocytes was analyzed before and after meiotic maturation using quick-freeze, deep-etch, rotary-shadow electron microscopy. The perivitelline space (PS) of the meiotically immature oocyte contains a filamentous network which connects microvilli (MV) and follicle cell macrovill...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(89)80044-2
更新日期：1989-01-01 00:00:00
abstract：:The early mouse embryo undertakes two types of cell division: symmetric that gives rise to the trophectoderm and then placenta or asymmetric that gives rise to inner cells that generate the embryo proper. Although cell division orientation is important, the mechanism regulating it has remained unclear. Here, we identi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.05.009
更新日期：2014-08-15 00:00:00
abstract：:GDF11, a new member of the TGF-beta gene superfamily, regulates anterior/posterior patterning in the axial skeleton during mouse embryogenesis. Gdf11 null mice display skeletal abnormalities that appear to represent anterior homeotic transformations of vertebrae consistent with high levels of Gdf11 expression in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9981
更新日期：2001-01-15 00:00:00
abstract：:The germinal vesicle (GV) of starfish oocytes stays just beneath the oocyte cortex at the presumptive animal pole during the long period of oogenesis. We subjected oocytes to a centrifugal force field to detach the GV from the cortex. The association between the cortex and the GV persisted and withstood a small amount...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9577
更新日期：2000-02-15 00:00:00
abstract：:The vertebrate liver and heart arise from adjacent cell layers in the anterior lateral (AL) endoderm and mesoderm of late gastrula embryos, and the earliest stages of liver and heart development are interrelated through reciprocal tissue interactions. Although classical embryological studies performed several decades ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.019
更新日期：2004-04-15 00:00:00
abstract：:Nucleostemin (NS), a member of a family of nucleolar GTP-binding proteins, is highly expressed in proliferating cells such as stem and cancer cells and is involved in the control of cell cycle progression. Both depletion and overexpression of NS result in stabilization of the tumor suppressor p53 protein in vitro. Alt...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.04.028
更新日期：2011-07-15 00:00:00
