Opioid receptor-induced GTPgamma35S binding during mouse development.


:Although a large superfamily of G-protein-coupled receptors serves multiple functions, little is known about their functional activation during ontogeny. To examine the functional activation of the mu-opioid receptor (MOR) and the delta-opioid receptor (DOR) during development, sections of mouse embryos and fetuses from e11.5 until birth were treated with DAMGO and DPDPE, respectively, and the ability of these drugs to induce G-protein coupling was assessed by using GTPgamma(35)S binding autoradiography. MOR activation was first detected in the caudate-putamen (CPU) at e12.5, and by e15.5, activity had not only increased in this region but also expanded to include the midbrain, medial habenula, hypothalamus, pons, and medulla. DOR activity first appeared at e17.5 in the hypothalamus, pons, medial habenula, and medulla and at p1 in the CPU at levels noticeably less than those of the MOR. In general, MOR and DOR activation lagged only slightly behind the appearance of MOR-1 and DOR-1 mRNA but delayed activation was particularly pronounced in the trigeminal ganglia, where MOR-1 gene expression was first detected at e13.5, but MOR activity was not observed even at birth. Thus, the data demonstrate temporal and often region-specific differences in the appearance and magnitude of functional activity in cell groups expressing either the MOR-1 or DOR-1 genes, suggesting that interaction between the opioid receptors, G-proteins, and other signaling cofactors is developmentally regulated.


Dev Biol


Developmental biology


Nitsche JF,Pintar JE




Has Abstract


2003-01-01 00:00:00














  • The representation of heart development in the gene ontology.

    abstract::An understanding of heart development is critical in any systems biology approach to cardiovascular disease. The interpretation of data generated from high-throughput technologies (such as microarray and proteomics) is also essential to this approach. However, characterizing the role of genes in the processes underlyi...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Khodiyar VK,Hill DP,Howe D,Berardini TZ,Tweedie S,Talmud PJ,Breckenridge R,Bhattarcharya S,Riley P,Scambler P,Lovering RC

    更新日期:2011-06-01 00:00:00

  • Wnt-4 signaling is involved in the control of smooth muscle cell fate via Bmp-4 in the medullary stroma of the developing kidney.

    abstract::Wnt-4, a member of the Wnt family of secreted signaling molecules, is essential for nephrogenesis, but its expression in the presumptive medulla suggests additional developmental roles in kidney organogenesis. We demonstrate here that Wnt-4 signaling plays also a role in the determination of the fate of smooth muscle ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Itäranta P,Chi L,Seppänen T,Niku M,Tuukkanen J,Peltoketo H,Vainio S

    更新日期:2006-05-15 00:00:00

  • Neural development in Onychophora (velvet worms) suggests a step-wise evolution of segmentation in the nervous system of Panarthropoda.

    abstract::A fundamental question in biology is how animal segmentation arose during evolution. One particular challenge is to clarify whether segmental ganglia of the nervous system evolved once, twice, or several times within the Bilateria. As close relatives of arthropods, Onychophora play an important role in this debate sin...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Mayer G,Whitington PM

    更新日期:2009-11-01 00:00:00

  • Syndecan, a cell surface proteoglycan, exhibits a molecular polymorphism during lung development.

    abstract::Syndecan, a cell surface proteoglycan, binds multiple extracellular ligands, and is developmentally regulated in epithelial and mesenchymal tissues. The branching morphogenesis of embryonic lung is dependent on epithelial-mesenchymal interactions and, based on studies with inhibitors, on proteoglycan synthesis. To ass...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Brauker JH,Trautman MS,Bernfield M

    更新日期:1991-10-01 00:00:00

  • Essential mesenchymal role of small GTPase Rac1 in interdigital programmed cell death during limb development.

    abstract::Developing vertebrate limbs are often utilized as a model for studying pattern formation and morphogenetic cell death. Herein, we report that conditional deletion of Rac1, a member of the Rho family of proteins, in mouse limb bud mesenchyme led to skeletal deformities in the autopod and soft tissue syndactyly, with th...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Suzuki D,Yamada A,Amano T,Yasuhara R,Kimura A,Sakahara M,Tsumaki N,Takeda S,Tamura M,Nakamura M,Wada N,Nohno T,Shiroishi T,Aiba A,Kamijo R

    更新日期:2009-11-15 00:00:00

  • Completion of Meiosis I of preovulatory oocytes and facilitation of preimplantation embryo development by glial cell line-derived neurotrophic factor.

    abstract::Optimal maturation of oocytes and successful development of preimplantation embryos is essential for reproduction. We performed DNA microarray analyses of ovarian transcripts and identified glial cell line-derived neurotrophic factor (GDNF) secreted by cumulus, granulosa, and theca cells as an ovarian factor stimulate...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kawamura K,Ye Y,Kawamura N,Jing L,Groenen P,Gelpke MS,Rauch R,Hsueh AJ,Tanaka T

    更新日期:2008-03-01 00:00:00

  • The caudal-related homeobox genes cdx1a and cdx4 act redundantly to regulate hox gene expression and the formation of putative hematopoietic stem cells during zebrafish embryogenesis.

    abstract::The hox genes play a central role in organogenesis and are implicated in the formation of hematopoietic stem cells (HSCs). The cdx genes encode homeodomain transcription factors that act as master regulators of the hox genes. In zebrafish, mutations in cdx4 cause a severe, but not complete, deficit in embryonic blood ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Davidson AJ,Zon LI

    更新日期:2006-04-15 00:00:00

  • Increase in intracellular cAMP is a prerequisite signal for initiation of physiological oocyte meiotic maturation in the hydrozoan Cytaeis uchidae.

    abstract::In medusae of the hydrozoan Cytaeis uchidae, oocyte meiotic maturation and spawning occur as a consequence of dark-light transition. In this study, we investigated the mechanism underlying the initiation of meiotic maturation using in vitro (isolated oocytes from ovaries) and in vivo (ovarian oocytes in medusae) syste...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Takeda N,Kyozuka K,Deguchi R

    更新日期:2006-10-01 00:00:00

  • Induction of acetylcholine receptor synthesis and aggregation: partial purification of low-molecular-weight activity.

    abstract::We have studied the effect of saline and acid extracts of chick brain on the total number of acetylcholine (ACh) receptors and the number of receptor clusters in cultured chick muscle cells. Myotubes in 7-day cultures responded more rapidly to brain extract than did myotubes in 4-day cultures, so the older cells were ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Buc-Caron MH,Nystrom P,Fischbach GD

    更新日期:1983-02-01 00:00:00

  • Erratic deposition of agrin during the formation of Xenopus neuromuscular junctions in culture.

    abstract::In order to disclose the mechanism that regulate synapse development we compared the distributions of agrin, acetylcholine receptors (AChR) and a basal lamina heparan sulfate proteoglycan (HSPG) in sections and cultures prepared from Xenopus laevis and Ambystoma mexicanum embryos. While agrin, AChR and HSPG may accumu...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Anderson MJ,Shi ZQ,Grawel R,Zackson SL

    更新日期:1995-07-01 00:00:00

  • Two distinct classes of prechondrogenic cell types in the embryonic limb bud.

    abstract::Differences are demonstrated in the chondrogenic potential of cells derived from the distal and proximal halves of chick wing buds from as early as stage 23, prior to the appearance of overt cartilage differentiation. In high cell density cultures, cells obtained from the distal portions of stage 23 or 24 limb buds ar...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Swalla BJ,Owens EM,Linsenmayer TF,Solursh M

    更新日期:1983-05-01 00:00:00

  • Idgenes are required for morphogenesis and cellular patterning in the developing mammalian cochlea.

    abstract::Inhibitor of differentiation and DNA-binding (Id) proteins, Id1 to Id4, function in the regulation of cellular proliferation and differentiation. Id proteins have been shown to interact with bHLH proteins and other proteins involved in regulating cellular proliferation and differentiation, suggesting a widespread regu...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Sakamoto S,Tateya T,Omori K,Kageyama R

    更新日期:2020-04-15 00:00:00

  • Ancestral Myf5 gene activity in periocular connective tissue identifies a subset of fibro/adipogenic progenitors but does not connote a myogenic origin.

    abstract::Extraocular muscles (EOM) represent a unique muscle group that controls eye movements and originates from head mesoderm, while the more typically studied body and limb muscles are somite-derived. Aiming to investigate myogenic progenitors (satellite cells) in EOM versus limb and diaphragm of adult mice, we have been u...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Stuelsatz P,Shearer A,Yablonka-Reuveni Z

    更新日期:2014-01-15 00:00:00

  • Ventralization of an indirect developing hemichordate by NiCl₂ suggests a conserved mechanism of dorso-ventral (D/V) patterning in Ambulacraria (hemichordates and echinoderms).

    abstract::One of the earliest steps in embryonic development is the establishment of the future body axes. Morphological and molecular data place the Ambulacraria (echinoderms and hemichordates) within the Deuterostomia and as the sister taxon to chordates. Extensive work over the last decades in echinoid (sea urchins) echinode...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Röttinger E,Martindale MQ

    更新日期:2011-06-01 00:00:00

  • Homeobox-containing genes in teratocarcinoma embryoid bodies: a possible role for Hox-D12 (Hox-4.7) in establishing the extraembryonic endoderm lineage in the mouse.

    abstract::We wish to identify genes involved in mediating early lineage decisions in the mouse embryo. F9 teratocarcinoma cells treated with retinoic acid (RA) in suspension culture develop into embryoid bodies (EBs) with an outer layer of visceral endoderm. In order to identify genes that are involved in establishing this extr...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Labosky PA,Weir MP,Grabel LB

    更新日期:1993-09-01 00:00:00

  • A MAPK pathway is involved in the control of mitosis after fertilization of the sea urchin egg.

    abstract::Activation and role of mitogen-activated protein (MAP) kinase (MAPK) during mitosis are still matters of controversy in early embryos. We report here that an ERK-like protein is present and highly phosphorylated in unfertilized sea urchin eggs. This MAPK becomes dephosphorylated after fertilization and a small pool of...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Zhang WL,Huitorel P,Glass R,Fernandez-Serra M,Arnone MI,Chiri S,Picard A,Ciapa B

    更新日期:2005-06-01 00:00:00

  • Outgrowth by fin motor axons in wildtype and a finless mutant of the Japanese medaka fish.

    abstract::The outgrowth of motor axons to the developing pectoral fin of the Japanese medaka fish (Oryzias latipes) was investigated both in wildtype embryos and in the pectoral finless (pl) mutants in which adults are missing pectoral fins. Late in embryogenesis the pectoral fin is a simple limb which contains two antagonist m...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Okamoto H,Kuwada JY

    更新日期:1991-07-01 00:00:00

  • Drosophila Cyclin J is a mitotically stable Cdk1 partner without essential functions.

    abstract::Cyclin J is a cyclin family member that appears to have evolved before the metazoan radiation. Its evolutionary conservation argues for an important role but functional characterizations of Cyclin J have remained very limited. In Drosophila, Cyclin J is expressed only in females. Using transgenic Drosophila lines expr...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Althoff F,Viktorinová I,Kastl J,Lehner CF

    更新日期:2009-09-15 00:00:00

  • Expression of a novel histone 2B during mouse spermiogenesis.

    abstract::During mammalian spermiogenesis transitional proteins and protamines replace histones on the DNA as the chromatin condenses. While previous studies suggested that histone genes are inactive postmeiotically, we have shown both by steady-state RNA analysis and nuclear run-off transcription assays that histone 2b (H2b) t...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Moss SB,Challoner PB,Groudine M

    更新日期:1989-05-01 00:00:00

  • Emergence of the mature myosin phenotype in the rat diaphragm muscle.

    abstract::Immunohistochemical analysis of myosin heavy chain (MHC) isoform expression in perinatal and adult rat diaphragm muscles was performed with antibodies which permitted the identification of all known MHC isoforms found in typical rat muscles. Isoform switching, leading to the emergence of the adult phenotype, was more ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: LaFramboise WA,Daood MJ,Guthrie RD,Schiaffino S,Moretti P,Brozanski B,Ontell MP,Butler-Browne GS,Whalen RG,Ontell M

    更新日期:1991-03-01 00:00:00

  • The delayed entry of thoracic neural crest cells into the dorsolateral path is a consequence of the late emigration of melanogenic neural crest cells from the neural tube.

    abstract::Neural crest cells migrate along two pathways in the trunk: the ventral path, between the neural tube and somite, and the dorsolateral path, between the somite and overlying ectoderm. In avian embryos, ventral migration precedes dorsolateral migration by nearly 24 h, and the onset of dorsolateral migration coincides w...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Reedy MV,Faraco CD,Erickson CA

    更新日期:1998-08-15 00:00:00

  • Zebrafish chordin-like and chordin are functionally redundant in regulating patterning of the dorsoventral axis.

    abstract::Chordin is the prototype of a group of cysteine-rich domain-containing proteins that bind and modulate signaling of various TGFbeta-like ligands. Chordin-like 1 and 2 (CHL1 and 2) are two members of this group that have been described in human, mouse, and chick. However, in vivo roles for CHL1 and 2 in early developme...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Branam AM,Hoffman GG,Pelegri F,Greenspan DS

    更新日期:2010-05-15 00:00:00

  • Retarded postimplantation development of X0 mouse embryos: impact of the parental origin of the monosomic X chromosome.

    abstract::About 12-17% of the embryos obtained by mating mice carrying the In(X)1H or Paf mutations are of the 39,X (X0) genotype. Depending on the mutant mice used for mating, the monosomic X chromosome can be inherited from the paternal (XP) or the maternal (XM) parent. The XP0 embryos display developmental retardation at gas...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Jamieson RV,Tan SS,Tam PP

    更新日期:1998-09-01 00:00:00

  • Co-expression of multiple neurotransmitter enzyme genes in normal and immortalized sympathoadrenal progenitor cells.

    abstract::We have examined the expression of mRNAs encoding five major neurotransmitter-synthesizing enzymes in MAH cells, a clonal cell line derived by retroviral immortalization of a rat embryonic sympathoadrenal progenitor cell. These mRNAs include tyrosine hydroxylase (TH), choline acetyltransferase (ChAT), tryptophan hydro...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Vandenbergh DJ,Mori N,Anderson DJ

    更新日期:1991-11-01 00:00:00

  • The forkhead/winged-helix gene, Mf1, is necessary for the normal development of the cornea and formation of the anterior chamber in the mouse eye.

    abstract::Mf1, which encodes a winged-helix/forkhead transcription factor, is the murine homolog of human FKHL7, mutated in individuals with autosomal dominant inherited dysgenesis of the anterior segment of the eye (Axenfeld-Reiger anomaly). Mouse embryos homozygous for null mutations in Mf1 (Mf1(lacZ) and Mf1(ch)) show severe...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kidson SH,Kume T,Deng K,Winfrey V,Hogan BL

    更新日期:1999-07-15 00:00:00

  • Remodeling of insulin producing beta-cells during Xenopus laevis metamorphosis.

    abstract::Insulin-producing beta-cells are present as single cells or in small clusters distributed throughout the pancreas of the Xenopus laevis tadpole. During metamorphic climax when the exocrine pancreas dedifferentiates to progenitor cells, the beta-cells undergo two changes. Insulin mRNA is down regulated at the beginning...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Mukhi S,Horb ME,Brown DD

    更新日期:2009-04-15 00:00:00

  • Zebrafish puma mutant decouples pigment pattern and somatic metamorphosis.

    abstract::The genetic and developmental bases for trait expression and variation in adults are largely unknown. One system in which genes and cell behaviors underlying adult traits can be elucidated is the larval-to-adult transformation of zebrafish, Danio rerio. Metamorphosis in this and many other teleost fishes resembles amp...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Parichy DM,Turner JM

    更新日期:2003-04-15 00:00:00

  • Rac1 deficiency in the forebrain results in neural progenitor reduction and microcephaly.

    abstract::The Rho family of small GTPases has been implicated in many neurological disorders including mental retardation, but whether they are involved in primary microcephaly (microcephalia vera) is unknown. Here, we examine the role of Rac1 in mammalian neural progenitors and forebrain development by a conditional gene-targe...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Chen L,Melendez J,Campbell K,Kuan CY,Zheng Y

    更新日期:2009-01-01 00:00:00

  • Regulation of locomotion and motoneuron trajectory selection and targeting by the Drosophila homolog of Olig family transcription factors.

    abstract::During the development of locomotion circuits it is essential that motoneurons with distinct subtype identities select the correct trajectories and target muscles. In vertebrates, the generation of motoneurons and myelinating glia depends on Olig2, one of the five Olig family bHLH transcription factors. We investigate...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Oyallon J,Apitz H,Miguel-Aliaga I,Timofeev K,Ferreira L,Salecker I

    更新日期:2012-09-15 00:00:00

  • Developmental acquisition of genome-wide DNA methylation occurs prior to meiosis in male germ cells.

    abstract::The development of germ cells is a highly ordered process that begins during fetal growth and is completed in the adult. Epigenetic modifications that occur in germ cells are important for germ cell function and for post-fertilization embryonic development. We have previously shown that male germ cells in the adult mo...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Oakes CC,La Salle S,Smiraglia DJ,Robaire B,Trasler JM

    更新日期:2007-07-15 00:00:00