Abstract：
:Although a large superfamily of G-protein-coupled receptors serves multiple functions, little is known about their functional activation during ontogeny. To examine the functional activation of the mu-opioid receptor (MOR) and the delta-opioid receptor (DOR) during development, sections of mouse embryos and fetuses from e11.5 until birth were treated with DAMGO and DPDPE, respectively, and the ability of these drugs to induce G-protein coupling was assessed by using GTPgamma(35)S binding autoradiography. MOR activation was first detected in the caudate-putamen (CPU) at e12.5, and by e15.5, activity had not only increased in this region but also expanded to include the midbrain, medial habenula, hypothalamus, pons, and medulla. DOR activity first appeared at e17.5 in the hypothalamus, pons, medial habenula, and medulla and at p1 in the CPU at levels noticeably less than those of the MOR. In general, MOR and DOR activation lagged only slightly behind the appearance of MOR-1 and DOR-1 mRNA but delayed activation was particularly pronounced in the trigeminal ganglia, where MOR-1 gene expression was first detected at e13.5, but MOR activity was not observed even at birth. Thus, the data demonstrate temporal and often region-specific differences in the appearance and magnitude of functional activity in cell groups expressing either the MOR-1 or DOR-1 genes, suggesting that interaction between the opioid receptors, G-proteins, and other signaling cofactors is developmentally regulated.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Nitsche JF,Pintar JEdoi
10.1006/dbio.2002.0855subject
Has Abstractpub_date
2003-01-01 00:00:00pages
99-108issue
1eissn
0012-1606issn
1095-564Xpii
S0012160602908559journal_volume
253pub_type
杂志文章abstract：:An understanding of heart development is critical in any systems biology approach to cardiovascular disease. The interpretation of data generated from high-throughput technologies (such as microarray and proteomics) is also essential to this approach. However, characterizing the role of genes in the processes underlyi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.03.011
更新日期：2011-06-01 00:00:00
abstract：:Wnt-4, a member of the Wnt family of secreted signaling molecules, is essential for nephrogenesis, but its expression in the presumptive medulla suggests additional developmental roles in kidney organogenesis. We demonstrate here that Wnt-4 signaling plays also a role in the determination of the fate of smooth muscle ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.02.019
更新日期：2006-05-15 00:00:00
abstract：:A fundamental question in biology is how animal segmentation arose during evolution. One particular challenge is to clarify whether segmental ganglia of the nervous system evolved once, twice, or several times within the Bilateria. As close relatives of arthropods, Onychophora play an important role in this debate sin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.08.011
更新日期：2009-11-01 00:00:00
abstract：:Syndecan, a cell surface proteoglycan, binds multiple extracellular ligands, and is developmentally regulated in epithelial and mesenchymal tissues. The branching morphogenesis of embryonic lung is dependent on epithelial-mesenchymal interactions and, based on studies with inhibitors, on proteoglycan synthesis. To ass...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90286-c
更新日期：1991-10-01 00:00:00
abstract：:Developing vertebrate limbs are often utilized as a model for studying pattern formation and morphogenetic cell death. Herein, we report that conditional deletion of Rac1, a member of the Rho family of proteins, in mouse limb bud mesenchyme led to skeletal deformities in the autopod and soft tissue syndactyly, with th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.09.014
更新日期：2009-11-15 00:00:00
abstract：:Optimal maturation of oocytes and successful development of preimplantation embryos is essential for reproduction. We performed DNA microarray analyses of ovarian transcripts and identified glial cell line-derived neurotrophic factor (GDNF) secreted by cumulus, granulosa, and theca cells as an ovarian factor stimulate...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.12.029
更新日期：2008-03-01 00:00:00
abstract：:The hox genes play a central role in organogenesis and are implicated in the formation of hematopoietic stem cells (HSCs). The cdx genes encode homeodomain transcription factors that act as master regulators of the hox genes. In zebrafish, mutations in cdx4 cause a severe, but not complete, deficit in embryonic blood ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.01.003
更新日期：2006-04-15 00:00:00
abstract：:In medusae of the hydrozoan Cytaeis uchidae, oocyte meiotic maturation and spawning occur as a consequence of dark-light transition. In this study, we investigated the mechanism underlying the initiation of meiotic maturation using in vitro (isolated oocytes from ovaries) and in vivo (ovarian oocytes in medusae) syste...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.06.034
更新日期：2006-10-01 00:00:00
abstract：:We have studied the effect of saline and acid extracts of chick brain on the total number of acetylcholine (ACh) receptors and the number of receptor clusters in cultured chick muscle cells. Myotubes in 7-day cultures responded more rapidly to brain extract than did myotubes in 4-day cultures, so the older cells were ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90039-8
更新日期：1983-02-01 00:00:00
abstract：:In order to disclose the mechanism that regulate synapse development we compared the distributions of agrin, acetylcholine receptors (AChR) and a basal lamina heparan sulfate proteoglycan (HSPG) in sections and cultures prepared from Xenopus laevis and Ambystoma mexicanum embryos. While agrin, AChR and HSPG may accumu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1191
更新日期：1995-07-01 00:00:00
abstract：:Differences are demonstrated in the chondrogenic potential of cells derived from the distal and proximal halves of chick wing buds from as early as stage 23, prior to the appearance of overt cartilage differentiation. In high cell density cultures, cells obtained from the distal portions of stage 23 or 24 limb buds ar...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90063-5
更新日期：1983-05-01 00:00:00
abstract：:Inhibitor of differentiation and DNA-binding (Id) proteins, Id1 to Id4, function in the regulation of cellular proliferation and differentiation. Id proteins have been shown to interact with bHLH proteins and other proteins involved in regulating cellular proliferation and differentiation, suggesting a widespread regu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.12.006
更新日期：2020-04-15 00:00:00
abstract：:Extraocular muscles (EOM) represent a unique muscle group that controls eye movements and originates from head mesoderm, while the more typically studied body and limb muscles are somite-derived. Aiming to investigate myogenic progenitors (satellite cells) in EOM versus limb and diaphragm of adult mice, we have been u...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.08.010
更新日期：2014-01-15 00:00:00
abstract：:One of the earliest steps in embryonic development is the establishment of the future body axes. Morphological and molecular data place the Ambulacraria (echinoderms and hemichordates) within the Deuterostomia and as the sister taxon to chordates. Extensive work over the last decades in echinoid (sea urchins) echinode...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.03.030
更新日期：2011-06-01 00:00:00
abstract：:We wish to identify genes involved in mediating early lineage decisions in the mouse embryo. F9 teratocarcinoma cells treated with retinoic acid (RA) in suspension culture develop into embryoid bodies (EBs) with an outer layer of visceral endoderm. In order to identify genes that are involved in establishing this extr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1236
更新日期：1993-09-01 00:00:00
abstract：:Activation and role of mitogen-activated protein (MAP) kinase (MAPK) during mitosis are still matters of controversy in early embryos. We report here that an ERK-like protein is present and highly phosphorylated in unfertilized sea urchin eggs. This MAPK becomes dephosphorylated after fertilization and a small pool of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.03.008
更新日期：2005-06-01 00:00:00
abstract：:The outgrowth of motor axons to the developing pectoral fin of the Japanese medaka fish (Oryzias latipes) was investigated both in wildtype embryos and in the pectoral finless (pl) mutants in which adults are missing pectoral fins. Late in embryogenesis the pectoral fin is a simple limb which contains two antagonist m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90445-9
更新日期：1991-07-01 00:00:00
abstract：:Cyclin J is a cyclin family member that appears to have evolved before the metazoan radiation. Its evolutionary conservation argues for an important role but functional characterizations of Cyclin J have remained very limited. In Drosophila, Cyclin J is expressed only in females. Using transgenic Drosophila lines expr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.06.042
更新日期：2009-09-15 00:00:00
abstract：:During mammalian spermiogenesis transitional proteins and protamines replace histones on the DNA as the chromatin condenses. While previous studies suggested that histone genes are inactive postmeiotically, we have shown both by steady-state RNA analysis and nuclear run-off transcription assays that histone 2b (H2b) t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90299-6
更新日期：1989-05-01 00:00:00
abstract：:Immunohistochemical analysis of myosin heavy chain (MHC) isoform expression in perinatal and adult rat diaphragm muscles was performed with antibodies which permitted the identification of all known MHC isoforms found in typical rat muscles. Isoform switching, leading to the emergence of the adult phenotype, was more ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90473-g
更新日期：1991-03-01 00:00:00
abstract：:Neural crest cells migrate along two pathways in the trunk: the ventral path, between the neural tube and somite, and the dorsolateral path, between the somite and overlying ectoderm. In avian embryos, ventral migration precedes dorsolateral migration by nearly 24 h, and the onset of dorsolateral migration coincides w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8963
更新日期：1998-08-15 00:00:00
abstract：:Chordin is the prototype of a group of cysteine-rich domain-containing proteins that bind and modulate signaling of various TGFbeta-like ligands. Chordin-like 1 and 2 (CHL1 and 2) are two members of this group that have been described in human, mouse, and chick. However, in vivo roles for CHL1 and 2 in early developme...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.03.001
更新日期：2010-05-15 00:00:00
abstract：:About 12-17% of the embryos obtained by mating mice carrying the In(X)1H or Paf mutations are of the 39,X (X0) genotype. Depending on the mutant mice used for mating, the monosomic X chromosome can be inherited from the paternal (XP) or the maternal (XM) parent. The XP0 embryos display developmental retardation at gas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8972
更新日期：1998-09-01 00:00:00
abstract：:We have examined the expression of mRNAs encoding five major neurotransmitter-synthesizing enzymes in MAH cells, a clonal cell line derived by retroviral immortalization of a rat embryonic sympathoadrenal progenitor cell. These mRNAs include tyrosine hydroxylase (TH), choline acetyltransferase (ChAT), tryptophan hydro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90313-r
更新日期：1991-11-01 00:00:00
abstract：:Mf1, which encodes a winged-helix/forkhead transcription factor, is the murine homolog of human FKHL7, mutated in individuals with autosomal dominant inherited dysgenesis of the anterior segment of the eye (Axenfeld-Reiger anomaly). Mouse embryos homozygous for null mutations in Mf1 (Mf1(lacZ) and Mf1(ch)) show severe...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9314
更新日期：1999-07-15 00:00:00
abstract：:Insulin-producing beta-cells are present as single cells or in small clusters distributed throughout the pancreas of the Xenopus laevis tadpole. During metamorphic climax when the exocrine pancreas dedifferentiates to progenitor cells, the beta-cells undergo two changes. Insulin mRNA is down regulated at the beginning...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.01.038
更新日期：2009-04-15 00:00:00
abstract：:The genetic and developmental bases for trait expression and variation in adults are largely unknown. One system in which genes and cell behaviors underlying adult traits can be elucidated is the larval-to-adult transformation of zebrafish, Danio rerio. Metamorphosis in this and many other teleost fishes resembles amp...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00015-0
更新日期：2003-04-15 00:00:00
abstract：:The Rho family of small GTPases has been implicated in many neurological disorders including mental retardation, but whether they are involved in primary microcephaly (microcephalia vera) is unknown. Here, we examine the role of Rac1 in mammalian neural progenitors and forebrain development by a conditional gene-targe...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.10.023
更新日期：2009-01-01 00:00:00
abstract：:During the development of locomotion circuits it is essential that motoneurons with distinct subtype identities select the correct trajectories and target muscles. In vertebrates, the generation of motoneurons and myelinating glia depends on Olig2, one of the five Olig family bHLH transcription factors. We investigate...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.06.027
更新日期：2012-09-15 00:00:00
abstract：:The development of germ cells is a highly ordered process that begins during fetal growth and is completed in the adult. Epigenetic modifications that occur in germ cells are important for germ cell function and for post-fertilization embryonic development. We have previously shown that male germ cells in the adult mo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.05.002
更新日期：2007-07-15 00:00:00
