Abstract:
:Sequence analysis of cDNA clones encoding fibronectin (FN) from Xenopus laevis reveals extensive amino acid identities with other vertebrate FNs, including the presence of the Arg-Gly-Asp (RGD) cell attachment site in type III-10 and of a second, cell-binding site (EILDV) in the alternative spliced V region of the protein. These cDNAs have been used to study the expression of FN mRNAs during early development. Overall, levels of maternal FN mRNA remain constant until the mid- to late-gastrula stage when the accumulation of new FN transcripts is first apparent. RNase protection analyses reveal that the pattern of FN alternative splicing is similar to that reported for other species and does not change with the shift from maternal to zygotic mRNA expression. The cellular forms of the FN protein predominate in the early embryo with the EIIIA and EIIIB exons included in most mRNAs at this time. A comparison of V-region alternative splicing between embryonic and adult liver RNAs indicates a segment of 345 nucleotides that can be either completely excluded or included in mature FN transcripts but there is no evidence for additional V-region variants. Maternal mRNAs encoding alternatively spliced forms of FN can be specifically eliminated from Xenopus oocytes following the injection of antisense oligodeoxynucleotides into the cytoplasm, thereby making it possible to analyze the structure, composition, and function of FN mRNAs in early embryos.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
DeSimone DW,Norton PA,Hynes ROdoi
10.1016/0012-1606(92)90291-nsubject
Has Abstractpub_date
1992-02-01 00:00:00pages
357-69issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(92)90291-Njournal_volume
149pub_type
杂志文章abstract::Neuronal differentiation requires a coordinated intracellular response to diverse extracellular stimuli, but the role of specific signaling mechanisms in regulating this process is still poorly understood. Soluble guanylate cyclases (sGCs), which can be stimulated by diffusible free radical gasses such as nitric oxide...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9066
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journal_title:Developmental biology
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journal_title:Developmental biology
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2020.12.020
更新日期:2021-01-09 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90396-x
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2009-09-15 00:00:00
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.07.016
更新日期:2008-10-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.05.017
更新日期:2012-08-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.03.006
更新日期:2006-06-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.03.027
更新日期:2005-06-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.02.013
更新日期:2005-05-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章,评审
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更新日期:2019-04-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2019-11-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8833
更新日期:1998-03-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2009-11-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:1995-04-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.10.048
更新日期:2008-01-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:1999-04-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(05)80021-1
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