Abstract:
:The mouse protamine mRNAs, Prm-1 and Prm-2, are translationally repressed for several days during male germ cell differentiation. The translational delay of mouse Prm-1 mRNA has previously been shown to be dependent upon cis-acting elements that reside in the last 62 nucleotides of the Prm-1 3' untranslated region (3' UTR). We have previously identified a 48/50-kDa protein that binds the 3' UTRs of both Prm-1 and Prm-2 mRNAs in a sequence-specific manner, is present in cytoplasmic fractions of postmeiotic round spermatids where the protamine mRNAs are translationally silent, and is markedly reduced in elongated spermatids where the protamine mRNAs become activated for translation. Surprisingly, the binding site for this activity maps to a region of the Prm-1 3' UTR not contained within the functional 62 nucleotides described above. In this report we show that the binding site for the 48/50-kDa protein can also delay translation of a reporter RNA in vivo, suggesting that the 48/50-kDa protein can repress the translation of Prm-1 mRNA during murine spermatogenesis. This observation proves that two separate regions of the Prm-1 3' UTR are sufficient to repress Prm-1 translation. In addition, immunocytochemistry and polysome analysis have revealed that this transgenic reporter mRNA fails to undergo proper translational activation. These results suggest that an additional region of the Prm-1 3' UTR is required for proper translational activation and that Prm-1 translational repression elements can be separated from those involved in translational activation.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Fajardo MA,Haugen HS,Clegg CH,Braun REdoi
10.1006/dbio.1997.8705subject
Has Abstractpub_date
1997-11-01 00:00:00pages
42-52issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(97)98705-4journal_volume
191pub_type
杂志文章abstract::John W. Saunders, Jr. made seminal discoveries unveiling how chick embryos develop their limbs. He discovered the apical ectodermal ridge (AER), the zone of polarizing activity (ZPA), and the domains of interdigital cell death within the developing limb and determined their function through experimental analysis. Thes...
journal_title:Developmental biology
pub_type: 杂志文章,评审
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
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abstract::The mab-21 gene was first identified because of its requirement for ray identity specification in Caenorhabditis elegans. It is now known to constitute a family of genes that are highly conserved from vertebrates to invertebrates, and two homologues Mab21l1 and Mab21l2 have been identified in many species. Here we des...
journal_title:Developmental biology
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abstract::Fibronectin (Fn) plays an important part in the branching morphogenesis of salivary gland, lung, and kidney. Here, we examine the effect of the conditional knockout of Fn in the mammary epithelium [Fn(MEp-/-)] on postnatal mammary gland development, using Cre-loxP-mediated gene knockout technology. Our data show that ...
journal_title:Developmental biology
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journal_title:Developmental biology
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abstract::The embryonic vertebrate heart consists of two epithelia: the myocardium and endothelium, separated by the myocardial basement membrane (MBM). The myocardium has been shown to induce endothelial transformation into prevalvular mesenchyme in a temporally and site restricted manner. Previously, we hypothesized that the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90135-8
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abstract::In Hydra long-range interactions between head, bud, and foot formation take place in addition to short-range interactions which are recognized as induction phenomena, occurring when transplants are placed into an environment of disparate positional value. Here the long-range promotion of foot formation is analyzed. Th...
journal_title:Developmental biology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2016-11-15 00:00:00
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doi:10.1016/j.ydbio.2004.01.048
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00139-8
更新日期:2003-06-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.11.009
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.07.009
更新日期:2011-09-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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doi:10.1016/j.ydbio.2015.03.024
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.08.014
更新日期:2017-10-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1198
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.03.014
更新日期:2016-05-15 00:00:00
abstract::Bullous pemphigoid antisera and monoclonal antibodies to type VII collagen were used to localize hemidesmosomes and anchoring fibrils, respectively, in tissues of developing eyes and healing corneal wounds of New Zealand white rabbits. In the 17-day fetal rabbit eye, both antibodies colocalize to the epithelial-stroma...
journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2011.03.015
更新日期:2011-06-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90372-p
更新日期:1990-12-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.02.002
更新日期:2006-05-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0416
更新日期:2001-11-01 00:00:00
abstract::The nematode gonad is an exemplary system for the study of organogenesis and fundamental problems in developmental and cellular biology. Nematode gonads vary dramatically across species (Chitwood, B.G., Chitwood, M.B., 1950. Introduction to Nematology." University Park Press, Baltimore; Felix, M.A., Sternberg, P.W., 1...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.09.021
更新日期:2005-01-01 00:00:00
abstract::The dysgenetic lens (dyl) mouse mutant has mutations in Foxe3, which inactivate DNA binding by the encoded forkhead transcription factor. Here we confirm, by targeted inactivation, that Foxe3 mutations are responsible for the dyl phenotype, which include loss of lens epithelium; a small, cataractic lens; and failure o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.09.021
更新日期:2007-02-01 00:00:00